TWI747808B - 新穎之ｃｈｏ整合位點及其用途 - Google Patents

新穎之ｃｈｏ整合位點及其用途 Download PDF

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TWI747808B
TWI747808B TW104134716A TW104134716A TWI747808B TW I747808 B TWI747808 B TW I747808B TW 104134716 A TW104134716 A TW 104134716A TW 104134716 A TW104134716 A TW 104134716A TW I747808 B TWI747808 B TW I747808B
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exogenous
seq
lox
locus
goi
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TW104134716A
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TW201629229A (zh
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沈盈
達里亞 布拉科夫
剛 陳
詹姆斯 凡德爾
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美商再生元醫藥公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

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TW104134716A 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途 TWI747808B (zh)

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US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23

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TWI747808B true TWI747808B (zh) 2021-12-01

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TW104134716A TWI747808B (zh) 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途
TW110128495A TWI780847B (zh) 2014-10-23 2015-10-22 新穎之ｃｈｏ整合位點及其用途

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US (5) US9816110B2 (enExample)
EP (1) EP3209785B1 (enExample)
JP (1) JP6668340B2 (enExample)
KR (1) KR102243243B1 (enExample)
CN (1) CN107109434A (enExample)
AR (1) AR102420A1 (enExample)
AU (1) AU2015335921B2 (enExample)
BR (1) BR112017008022A2 (enExample)
CA (1) CA2965495C (enExample)
EA (1) EA037255B1 (enExample)
IL (1) IL251674B (enExample)
MX (1) MX2017005306A (enExample)
MY (1) MY184103A (enExample)
SG (1) SG11201703149RA (enExample)
TW (2) TWI747808B (enExample)
WO (1) WO2016064999A1 (enExample)

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SG10202010156XA (en) * 2016-04-20 2020-11-27 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
EP3559236A4 (en) * 2016-12-20 2020-11-04 Development Center for Biotechnology TARGETED INTEGRATION SITES IN THE CHINESE HAMSTER OVARIAN CELL GENOME
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
US11781116B2 (en) * 2017-02-17 2023-10-10 Lonza Ltd. Mammalian cells for producing adeno-associated viruses
WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
US11560549B2 (en) 2017-08-11 2023-01-24 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
WO2019126562A1 (en) * 2017-12-22 2019-06-27 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B2 (en) * 2017-12-22 2026-01-01 Genentech Inc Targeted integration of nucleic acids
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CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN113631711B (zh) * 2018-12-21 2025-03-04 豪夫迈·罗氏有限公司 核酸的靶向整合
EP3950938A4 (en) * 2019-04-02 2023-01-18 Chugai Seiyaku Kabushiki Kaisha PROCEDURE FOR INTRODUCING A TARGET-SPECIFIC ALIEN
AU2020308002A1 (en) 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
BR112022024644A2 (pt) * 2020-06-02 2023-02-23 Catalent Pharma Solutions Llc Linhagens celulares com múltiplas ancoragens para inserção de gene
EP4172346A1 (en) * 2020-06-24 2023-05-03 Genentech, Inc. Targeted integration of nucleic acids
KR20230085929A (ko) 2020-10-15 2023-06-14 에프. 호프만-라 로슈 아게 Va rna 전사를 위한 핵산 구조체
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
US20230304062A1 (en) 2021-10-18 2023-09-28 Regeneron Pharmaceuticals, Inc. Controlled transcription of polynucleotides
US12234472B2 (en) 2021-10-18 2025-02-25 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
TW202334402A (zh) 2021-10-18 2023-09-01 美商再生元醫藥公司 包含整合ｃａｓ９基因以產生穩定整合位點的哺乳動物細胞，以及包含穩定整合位點及其他位點的哺乳動物細胞
AU2023213996A1 (en) * 2022-01-28 2024-06-27 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
US20240168018A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
CN121794393A (zh) 2023-09-07 2026-04-03 里珍纳龙药品有限公司 经共价表面修饰的腺相关病毒的生产和纯化
TW202540433A (zh) 2023-11-21 2025-10-16 美商再生元醫藥公司 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化
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KR20170096999A (ko) 2017-08-25
US20160115502A1 (en) 2016-04-28
AR102420A1 (es) 2017-03-01
EA037255B1 (ru) 2021-02-26
US20180030480A1 (en) 2018-02-01
MX2017005306A (es) 2018-01-09
US9816110B2 (en) 2017-11-14
IL251674B (en) 2020-10-29
SG11201703149RA (en) 2018-02-27
US12565667B2 (en) 2026-03-03
EP3209785A1 (en) 2017-08-30
AU2015335921A1 (en) 2017-05-04
IL251674A0 (en) 2017-06-29
US20210171984A1 (en) 2021-06-10
MY184103A (en) 2021-03-18
US20200002732A1 (en) 2020-01-02
TWI780847B (zh) 2022-10-11
KR102243243B1 (ko) 2021-04-22
JP6668340B2 (ja) 2020-03-18
TW201629229A (zh) 2016-08-16
US20240018553A1 (en) 2024-01-18
CN107109434A (zh) 2017-08-29
BR112017008022A2 (pt) 2018-02-20
CA2965495A1 (en) 2016-04-28
US11788102B2 (en) 2023-10-17
TW202144576A (zh) 2021-12-01
WO2016064999A1 (en) 2016-04-28
CA2965495C (en) 2022-05-24
EA201790698A1 (ru) 2018-01-31
EP3209785B1 (en) 2019-07-31
JP2017535258A (ja) 2017-11-30
AU2015335921B2 (en) 2020-06-25
US11268109B2 (en) 2022-03-08

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