JP6668340B2 - 新規ｃｈｏ組込み部位およびその使用 - Google Patents

新規ｃｈｏ組込み部位およびその使用 Download PDF

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JP6668340B2
JP6668340B2 JP2017519646A JP2017519646A JP6668340B2 JP 6668340 B2 JP6668340 B2 JP 6668340B2 JP 2017519646 A JP2017519646 A JP 2017519646A JP 2017519646 A JP2017519646 A JP 2017519646A JP 6668340 B2 JP6668340 B2 JP 6668340B2
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exogenous
seq
goi
nucleic acid
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JP2017535258A (ja
JP2017535258A5 (enExample
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イン シェン，
イン シェン，
ダリャ ブラコフ，
ダリャ ブラコフ，
ガン チェン，
ガン チェン，
ジェイムズ ピー． ファンデル，
ジェイムズ ピー． ファンデル，
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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JP2017519646A 2014-10-23 2015-10-21 新規ｃｈｏ組込み部位およびその使用 Active JP6668340B2 (ja)

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Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

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JP2017535258A JP2017535258A (ja) 2017-11-30
JP2017535258A5 JP2017535258A5 (enExample) 2018-11-22
JP6668340B2 true JP6668340B2 (ja) 2020-03-18

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US (5) US9816110B2 (enExample)
EP (1) EP3209785B1 (enExample)
JP (1) JP6668340B2 (enExample)
KR (1) KR102243243B1 (enExample)
CN (1) CN107109434A (enExample)
AR (1) AR102420A1 (enExample)
AU (1) AU2015335921B2 (enExample)
BR (1) BR112017008022A2 (enExample)
CA (1) CA2965495C (enExample)
EA (1) EA037255B1 (enExample)
IL (1) IL251674B (enExample)
MX (1) MX2017005306A (enExample)
MY (1) MY184103A (enExample)
SG (1) SG11201703149RA (enExample)
TW (2) TWI747808B (enExample)
WO (1) WO2016064999A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12344859B2 (en) 2019-04-02 2025-07-01 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene

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US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US11512144B2 (en) 2016-04-20 2022-11-29 Regeneran Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing loci
IL262268B2 (en) * 2016-04-20 2024-05-01 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
EP3559236A4 (en) * 2016-12-20 2020-11-04 Development Center for Biotechnology TARGETED INTEGRATION SITES IN THE CHINESE HAMSTER OVARIAN CELL GENOME
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
EP3583205A1 (en) * 2017-02-17 2019-12-25 Lonza Ltd Mammalian cells for producing adeno-associated viruses
EP3601542A4 (en) * 2017-03-19 2021-01-13 Applied Stemcell, Inc. NEW INTEGRATION SITES AND THEIR USES
JP7087061B2 (ja) * 2017-08-11 2022-06-20 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Ｃｈｏ細胞内の組込み部位
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
TW201932600A (zh) * 2017-12-22 2019-08-16 美商建南德克公司 核酸之靶向整合
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
KR20210107057A (ko) * 2018-12-21 2021-08-31 제넨테크, 인크. 핵산의 표적화 통합
CN114080454A (zh) 2019-06-26 2022-02-22 豪夫迈·罗氏有限公司 核酸的随机化构型靶向整合
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
EP4158041A4 (en) * 2020-06-02 2024-09-25 Catalent Pharma Solutions, LLC CELL LINES WITH MULTIPLE DOCKING ZONES FOR GENE INSERTION
TW202216991A (zh) * 2020-06-24 2022-05-01 美商建南德克公司 核酸之靶向整合
IL302046A (en) 2020-10-15 2023-06-01 Hoffmann La Roche Nucleic acid structures for va RNA transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
KR20240099242A (ko) 2021-10-18 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 안정적인 통합 부위를 생산하기 위한 통합된 cas9 유전자를 포함하는 포유동물 세포, 및 안정적인 통합 부위 및 기타 부위를 포함하는 포유동물 세포
MX2024004647A (es) 2021-10-18 2024-05-02 Regeneron Pharma Celulas eucariotas que comprenden polinucleotidos de virus asociados a adenovirus.
KR20240101596A (ko) 2021-10-18 2024-07-02 리제너론 파마슈티칼스 인코포레이티드 폴리뉴클레오티드의 제어된 전사
WO2023147462A2 (en) * 2022-01-28 2023-08-03 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
WO2024107451A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12344859B2 (en) 2019-04-02 2025-07-01 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene

Also Published As

Publication number Publication date
KR20170096999A (ko) 2017-08-25
KR102243243B1 (ko) 2021-04-22
TWI747808B (zh) 2021-12-01
EP3209785B1 (en) 2019-07-31
EP3209785A1 (en) 2017-08-30
AU2015335921A1 (en) 2017-05-04
TW202144576A (zh) 2021-12-01
SG11201703149RA (en) 2018-02-27
CA2965495C (en) 2022-05-24
US20160115502A1 (en) 2016-04-28
MY184103A (en) 2021-03-18
US11268109B2 (en) 2022-03-08
CA2965495A1 (en) 2016-04-28
US20180030480A1 (en) 2018-02-01
EA037255B1 (ru) 2021-02-26
US20210171984A1 (en) 2021-06-10
US20240018553A1 (en) 2024-01-18
CN107109434A (zh) 2017-08-29
US9816110B2 (en) 2017-11-14
TW201629229A (zh) 2016-08-16
WO2016064999A1 (en) 2016-04-28
IL251674A0 (en) 2017-06-29
AU2015335921B2 (en) 2020-06-25
EA201790698A1 (ru) 2018-01-31
MX2017005306A (es) 2018-01-09
TWI780847B (zh) 2022-10-11
US20200002732A1 (en) 2020-01-02
JP2017535258A (ja) 2017-11-30
BR112017008022A2 (pt) 2018-02-20
IL251674B (en) 2020-10-29
US11788102B2 (en) 2023-10-17
AR102420A1 (es) 2017-03-01

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