JP6668340B2 - 新規ｃｈｏ組込み部位およびその使用 - Google Patents

新規ｃｈｏ組込み部位およびその使用 Download PDF

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JP6668340B2
JP6668340B2 JP2017519646A JP2017519646A JP6668340B2 JP 6668340 B2 JP6668340 B2 JP 6668340B2 JP 2017519646 A JP2017519646 A JP 2017519646A JP 2017519646 A JP2017519646 A JP 2017519646A JP 6668340 B2 JP6668340 B2 JP 6668340B2
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exogenous
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goi
nucleic acid
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JP2017535258A (ja
JP2017535258A5 (enExample
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イン シェン，
イン シェン，
ダリャ ブラコフ，
ダリャ ブラコフ，
ガン チェン，
ガン チェン，
ジェイムズ ピー． ファンデル，
ジェイムズ ピー． ファンデル，
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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JP2017519646A 2014-10-23 2015-10-21 新規ｃｈｏ組込み部位およびその使用 Active JP6668340B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

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JP2017535258A JP2017535258A (ja) 2017-11-30
JP2017535258A5 JP2017535258A5 (enExample) 2018-11-22
JP6668340B2 true JP6668340B2 (ja) 2020-03-18

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JP2017519646A Active JP6668340B2 (ja) 2014-10-23 2015-10-21 新規ｃｈｏ組込み部位およびその使用

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US (5) US9816110B2 (enExample)
EP (1) EP3209785B1 (enExample)
JP (1) JP6668340B2 (enExample)
KR (1) KR102243243B1 (enExample)
CN (1) CN107109434A (enExample)
AR (1) AR102420A1 (enExample)
AU (1) AU2015335921B2 (enExample)
BR (1) BR112017008022A2 (enExample)
CA (1) CA2965495C (enExample)
EA (1) EA037255B1 (enExample)
IL (1) IL251674B (enExample)
MX (1) MX2017005306A (enExample)
MY (1) MY184103A (enExample)
SG (1) SG11201703149RA (enExample)
TW (2) TWI780847B (enExample)
WO (1) WO2016064999A1 (enExample)

Cited By (1)

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US12344859B2 (en) 2019-04-02 2025-07-01 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene

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KR102243243B1 (ko) 2014-10-23 2021-04-22 리제너론 파마슈티칼스 인코포레이티드 신규한 cho 통합 부위 및 이의 용도
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
EP3445780A1 (en) * 2016-04-20 2019-02-27 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing locus
TWI827531B (zh) * 2016-04-20 2024-01-01 美商再生元醫藥公司 基於使用增強表現基因座之製備抗體的組成物及方法
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
US11499139B2 (en) * 2016-12-20 2022-11-15 Development Center For Biotechnology Targeted integration sites in Chinese hamster ovary cell genome
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
CA3053304A1 (en) * 2017-02-17 2018-08-23 Lonza Ltd. Mammalian cells for producing adeno-associated viruses
WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
EP3665291B1 (en) * 2017-08-11 2021-10-06 Boehringer Ingelheim International GmbH Integration sites in cho cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
IL275462B1 (en) * 2017-12-22 2025-09-01 Genentech Inc Targeted integration of nucleic acids
EP3728581B1 (en) * 2017-12-22 2024-10-09 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
AU2020308002A1 (en) 2019-06-26 2022-01-20 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
EP4158041A4 (en) * 2020-06-02 2024-09-25 Catalent Pharma Solutions, LLC CELL LINES WITH MULTIPLE DOCKING ZONES FOR GENE INSERTION
JP2023532665A (ja) * 2020-06-24 2023-07-31 ジェネンテック， インコーポレイテッド 核酸の標的化組み込み
CA3197730A1 (en) 2020-10-15 2022-04-21 F. Hoffman-La Roche Ag Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
EP4419698A1 (en) 2021-10-18 2024-08-28 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
AU2022373653A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
US20230304062A1 (en) 2021-10-18 2023-09-28 Regeneron Pharmaceuticals, Inc. Controlled transcription of polynucleotides
AU2023213996A1 (en) * 2022-01-28 2024-06-27 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
US20240167984A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and determining protein structures and stability in fluids, including biological fluids
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
US20250122482A1 (en) 2023-09-07 2025-04-17 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12344859B2 (en) 2019-04-02 2025-07-01 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene

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US9816110B2 (en) 2017-11-14
SG11201703149RA (en) 2018-02-27
CN107109434A (zh) 2017-08-29
US20240018553A1 (en) 2024-01-18
US20210171984A1 (en) 2021-06-10
EA201790698A1 (ru) 2018-01-31
AU2015335921B2 (en) 2020-06-25
CA2965495A1 (en) 2016-04-28
BR112017008022A2 (pt) 2018-02-20
TWI780847B (zh) 2022-10-11
CA2965495C (en) 2022-05-24
AU2015335921A1 (en) 2017-05-04
MY184103A (en) 2021-03-18
JP2017535258A (ja) 2017-11-30
TWI747808B (zh) 2021-12-01
TW201629229A (zh) 2016-08-16
EP3209785B1 (en) 2019-07-31
EA037255B1 (ru) 2021-02-26
TW202144576A (zh) 2021-12-01
US20160115502A1 (en) 2016-04-28
WO2016064999A1 (en) 2016-04-28
IL251674B (en) 2020-10-29
US11788102B2 (en) 2023-10-17
MX2017005306A (es) 2018-01-09
EP3209785A1 (en) 2017-08-30
US20200002732A1 (en) 2020-01-02
KR102243243B1 (ko) 2021-04-22
US20180030480A1 (en) 2018-02-01
IL251674A0 (en) 2017-06-29
US11268109B2 (en) 2022-03-08
KR20170096999A (ko) 2017-08-25
AR102420A1 (es) 2017-03-01

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