KR102243243B1 - 신규한 cho 통합 부위 및 이의 용도 - Google Patents

신규한 cho 통합 부위 및 이의 용도 Download PDF

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KR102243243B1
KR102243243B1 KR1020177012465A KR20177012465A KR102243243B1 KR 102243243 B1 KR102243243 B1 KR 102243243B1 KR 1020177012465 A KR1020177012465 A KR 1020177012465A KR 20177012465 A KR20177012465 A KR 20177012465A KR 102243243 B1 KR102243243 B1 KR 102243243B1
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KR20170096999A (ko
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잉 셴
다리야 부라코프
강 첸
제임스 피. 팬들
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리제너론 파마슈티칼스 인코포레이티드
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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KR1020177012465A 2014-10-23 2015-10-21 신규한 cho 통합 부위 및 이의 용도 Active KR102243243B1 (ko)

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Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
US62/067,774 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

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KR20170096999A KR20170096999A (ko) 2017-08-25
KR102243243B1 true KR102243243B1 (ko) 2021-04-22

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US (5) US9816110B2 (enExample)
EP (1) EP3209785B1 (enExample)
JP (1) JP6668340B2 (enExample)
KR (1) KR102243243B1 (enExample)
CN (1) CN107109434A (enExample)
AR (1) AR102420A1 (enExample)
AU (1) AU2015335921B2 (enExample)
BR (1) BR112017008022A2 (enExample)
CA (1) CA2965495C (enExample)
EA (1) EA037255B1 (enExample)
IL (1) IL251674B (enExample)
MX (1) MX2017005306A (enExample)
MY (1) MY184103A (enExample)
SG (1) SG11201703149RA (enExample)
TW (2) TWI747808B (enExample)
WO (1) WO2016064999A1 (enExample)

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US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US11512144B2 (en) 2016-04-20 2022-11-29 Regeneran Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing loci
IL262268B2 (en) * 2016-04-20 2024-05-01 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
EP3559236A4 (en) * 2016-12-20 2020-11-04 Development Center for Biotechnology TARGETED INTEGRATION SITES IN THE CHINESE HAMSTER OVARIAN CELL GENOME
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
EP3583205A1 (en) * 2017-02-17 2019-12-25 Lonza Ltd Mammalian cells for producing adeno-associated viruses
EP3601542A4 (en) * 2017-03-19 2021-01-13 Applied Stemcell, Inc. NEW INTEGRATION SITES AND THEIR USES
JP7087061B2 (ja) * 2017-08-11 2022-06-20 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Cho細胞内の組込み部位
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
TW201932600A (zh) * 2017-12-22 2019-08-16 美商建南德克公司 核酸之靶向整合
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
KR20210107057A (ko) * 2018-12-21 2021-08-31 제넨테크, 인크. 핵산의 표적화 통합
IL286630B2 (en) * 2019-04-02 2025-05-01 Chugai Pharmaceutical Co Ltd Method for introducing a target-specific foreign gene
CN114080454A (zh) 2019-06-26 2022-02-22 豪夫迈·罗氏有限公司 核酸的随机化构型靶向整合
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
EP4158041A4 (en) * 2020-06-02 2024-09-25 Catalent Pharma Solutions, LLC CELL LINES WITH MULTIPLE DOCKING ZONES FOR GENE INSERTION
TW202216991A (zh) * 2020-06-24 2022-05-01 美商建南德克公司 核酸之靶向整合
IL302046A (en) 2020-10-15 2023-06-01 Hoffmann La Roche Nucleic acid structures for va RNA transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
KR20240099242A (ko) 2021-10-18 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 안정적인 통합 부위를 생산하기 위한 통합된 cas9 유전자를 포함하는 포유동물 세포, 및 안정적인 통합 부위 및 기타 부위를 포함하는 포유동물 세포
MX2024004647A (es) 2021-10-18 2024-05-02 Regeneron Pharma Celulas eucariotas que comprenden polinucleotidos de virus asociados a adenovirus.
KR20240101596A (ko) 2021-10-18 2024-07-02 리제너론 파마슈티칼스 인코포레이티드 폴리뉴클레오티드의 제어된 전사
WO2023147462A2 (en) * 2022-01-28 2023-08-03 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
WO2024107451A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

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Publication number Publication date
KR20170096999A (ko) 2017-08-25
TWI747808B (zh) 2021-12-01
EP3209785B1 (en) 2019-07-31
EP3209785A1 (en) 2017-08-30
AU2015335921A1 (en) 2017-05-04
TW202144576A (zh) 2021-12-01
SG11201703149RA (en) 2018-02-27
JP6668340B2 (ja) 2020-03-18
CA2965495C (en) 2022-05-24
US20160115502A1 (en) 2016-04-28
MY184103A (en) 2021-03-18
US11268109B2 (en) 2022-03-08
CA2965495A1 (en) 2016-04-28
US20180030480A1 (en) 2018-02-01
EA037255B1 (ru) 2021-02-26
US20210171984A1 (en) 2021-06-10
US20240018553A1 (en) 2024-01-18
CN107109434A (zh) 2017-08-29
US9816110B2 (en) 2017-11-14
TW201629229A (zh) 2016-08-16
WO2016064999A1 (en) 2016-04-28
IL251674A0 (en) 2017-06-29
AU2015335921B2 (en) 2020-06-25
EA201790698A1 (ru) 2018-01-31
MX2017005306A (es) 2018-01-09
TWI780847B (zh) 2022-10-11
US20200002732A1 (en) 2020-01-02
JP2017535258A (ja) 2017-11-30
BR112017008022A2 (pt) 2018-02-20
IL251674B (en) 2020-10-29
US11788102B2 (en) 2023-10-17
AR102420A1 (es) 2017-03-01

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