BR112017008022A2 - célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse. - Google Patents
célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse.Info
- Publication number
- BR112017008022A2 BR112017008022A2 BR112017008022-2A BR112017008022A BR112017008022A2 BR 112017008022 A2 BR112017008022 A2 BR 112017008022A2 BR 112017008022 A BR112017008022 A BR 112017008022A BR 112017008022 A2 BR112017008022 A2 BR 112017008022A2
- Authority
- BR
- Brazil
- Prior art keywords
- expression
- interest
- methods
- cell
- polynucleotide
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 230000014509 gene expression Effects 0.000 abstract 5
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000002759 chromosomal effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 230000010473 stable expression Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462067774P | 2014-10-23 | 2014-10-23 | |
| US62/067774 | 2014-10-23 | ||
| PCT/US2015/056653 WO2016064999A1 (en) | 2014-10-23 | 2015-10-21 | Novel cho integration sites and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017008022A2 true BR112017008022A2 (pt) | 2018-02-20 |
Family
ID=54602002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017008022-2A BR112017008022A2 (pt) | 2014-10-23 | 2015-10-21 | célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse. |
Country Status (16)
| Country | Link |
|---|---|
| US (5) | US9816110B2 (enExample) |
| EP (1) | EP3209785B1 (enExample) |
| JP (1) | JP6668340B2 (enExample) |
| KR (1) | KR102243243B1 (enExample) |
| CN (1) | CN107109434A (enExample) |
| AR (1) | AR102420A1 (enExample) |
| AU (1) | AU2015335921B2 (enExample) |
| BR (1) | BR112017008022A2 (enExample) |
| CA (1) | CA2965495C (enExample) |
| EA (1) | EA037255B1 (enExample) |
| IL (1) | IL251674B (enExample) |
| MX (1) | MX2017005306A (enExample) |
| MY (1) | MY184103A (enExample) |
| SG (1) | SG11201703149RA (enExample) |
| TW (2) | TWI780847B (enExample) |
| WO (1) | WO2016064999A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102243243B1 (ko) | 2014-10-23 | 2021-04-22 | 리제너론 파마슈티칼스 인코포레이티드 | 신규한 cho 통합 부위 및 이의 용도 |
| US10329594B1 (en) | 2015-09-03 | 2019-06-25 | CHO Plus, Inc. | Cell lines for high level production of protein-based pharmaceuticals |
| US11649449B2 (en) | 2015-09-03 | 2023-05-16 | CHO Plus, Inc. | Hybrid cell lines for high level production of a target protein |
| EP3445780A1 (en) * | 2016-04-20 | 2019-02-27 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| TWI827531B (zh) * | 2016-04-20 | 2024-01-01 | 美商再生元醫藥公司 | 基於使用增強表現基因座之製備抗體的組成物及方法 |
| WO2018039499A1 (en) | 2016-08-24 | 2018-03-01 | Regeneron Pharmaceuticals, Inc. | Host cell protein modification |
| US11499139B2 (en) * | 2016-12-20 | 2022-11-15 | Development Center For Biotechnology | Targeted integration sites in Chinese hamster ovary cell genome |
| WO2018148196A1 (en) * | 2017-02-07 | 2018-08-16 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| CA3053304A1 (en) * | 2017-02-17 | 2018-08-23 | Lonza Ltd. | Mammalian cells for producing adeno-associated viruses |
| WO2018175153A1 (en) * | 2017-03-19 | 2018-09-27 | Applied Stemcell, Inc. | Novel integration sites and uses thereof |
| EP3665291B1 (en) * | 2017-08-11 | 2021-10-06 | Boehringer Ingelheim International GmbH | Integration sites in cho cells |
| CN107723276B (zh) * | 2017-11-02 | 2021-08-13 | 上海交通大学 | 一种稳定高表达目标产物的细胞株的构建方法和试剂盒 |
| IL275462B1 (en) * | 2017-12-22 | 2025-09-01 | Genentech Inc | Targeted integration of nucleic acids |
| EP3728581B1 (en) * | 2017-12-22 | 2024-10-09 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| CN109207432B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用 |
| CN109295092B (zh) * | 2018-10-30 | 2021-06-29 | 江南大学 | 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用 |
| CN109136193B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用 |
| CN109337927B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用 |
| CN109321604B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用 |
| CN109295093B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用 |
| AU2019403279A1 (en) * | 2018-12-21 | 2021-07-01 | Genentech, Inc. | Targeted integration of nucleic acids |
| EP3950938A4 (en) | 2019-04-02 | 2023-01-18 | Chugai Seiyaku Kabushiki Kaisha | METHOD FOR INTRODUCING A FOREIGN TARGET-SPECIFIC GENE |
| AU2020308002A1 (en) | 2019-06-26 | 2022-01-20 | Genentech, Inc. | Randomized configuration targeted integration of nucleic acids |
| EP3901266A1 (en) * | 2020-04-22 | 2021-10-27 | LEK Pharmaceuticals d.d. | Super-enhancers for recombinant gene expression in cho cells |
| EP4158041A4 (en) * | 2020-06-02 | 2024-09-25 | Catalent Pharma Solutions, LLC | CELL LINES WITH MULTIPLE DOCKING ZONES FOR GENE INSERTION |
| JP2023532665A (ja) * | 2020-06-24 | 2023-07-31 | ジェネンテック， インコーポレイテッド | 核酸の標的化組み込み |
| CA3197730A1 (en) | 2020-10-15 | 2022-04-21 | F. Hoffman-La Roche Ag | Nucleic acid constructs for va rna transcription |
| CN113755447B (zh) * | 2021-09-23 | 2022-04-29 | 云舟生物科技(广州)股份有限公司 | 一种用于生产腺病毒的293a细胞株及其制备与应用 |
| EP4419698A1 (en) | 2021-10-18 | 2024-08-28 | Regeneron Pharmaceuticals, Inc. | Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites |
| AU2022373653A1 (en) | 2021-10-18 | 2024-05-02 | Regeneron Pharmaceuticals, Inc. | Eukaryotic cells comprising adenovirus-associated virus polynucleotides |
| US20230304062A1 (en) | 2021-10-18 | 2023-09-28 | Regeneron Pharmaceuticals, Inc. | Controlled transcription of polynucleotides |
| AU2023213996A1 (en) * | 2022-01-28 | 2024-06-27 | The Trustees Of Columbia University In The City Of New York | Genetic modifications for xenotransplantation |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
| US20240167984A1 (en) | 2022-11-18 | 2024-05-23 | Regeneron Pharmaceuticals, Inc. | Methods for detecting and determining protein structures and stability in fluids, including biological fluids |
| CN118726475B (zh) * | 2023-03-28 | 2025-07-08 | 深圳太力生物技术有限责任公司 | 核酸、重组载体、靶向整合细胞、基因表达方法和应用 |
| WO2024211287A1 (en) * | 2023-04-03 | 2024-10-10 | Seagen Inc. | Production cell lines with targeted integration sites |
| US20250122482A1 (en) | 2023-09-07 | 2025-04-17 | Regeneron Pharmaceuticals, Inc. | Production and purification of covalently surface modified adeno-associated virus |
| WO2025111473A1 (en) | 2023-11-21 | 2025-05-30 | Regeneron Pharmaceuticals, Inc. | Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| US6689606B2 (en) | 1998-07-21 | 2004-02-10 | M.L. Laboratories Plc | Polynucleotide |
| US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| AU2002254529A1 (en) | 2001-04-04 | 2002-10-21 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of | Nucleic acids for transgene expression |
| US20040045043A1 (en) * | 2002-05-20 | 2004-03-04 | Finney Robert E. | Compositions and methods for generating conditional knockouts |
| US8673589B2 (en) | 2002-05-29 | 2014-03-18 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
| WO2008151219A1 (en) | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| CN102625655B (zh) * | 2008-12-04 | 2016-07-06 | 桑格摩生物科学股份有限公司 | 使用锌指核酸酶在大鼠中进行基因组编辑 |
| CN103597083B (zh) | 2011-04-05 | 2016-05-18 | 斯克利普斯研究所 | 染色体着陆垫及相关用途 |
| PL2699264T3 (pl) | 2011-04-20 | 2018-08-31 | Medimmune, Llc | Przeciwciała i inne cząsteczki wiążące B7-H1 i PD-1 |
| DK3489366T3 (da) * | 2011-06-01 | 2020-02-24 | Prec Biosciences Inc | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
| CA2852955C (en) * | 2011-10-27 | 2021-02-16 | Sangamo Biosciences, Inc. | Methods and compositions for modification of the hprt locus |
| BR112014027813A2 (pt) | 2012-05-07 | 2017-08-08 | Dow Agrosciences Llc | métodos e composições para integração de transgenes direcionada mediada por nuclease |
| KR102243243B1 (ko) | 2014-10-23 | 2021-04-22 | 리제너론 파마슈티칼스 인코포레이티드 | 신규한 cho 통합 부위 및 이의 용도 |
-
2015
- 2015-10-21 KR KR1020177012465A patent/KR102243243B1/ko active Active
- 2015-10-21 JP JP2017519646A patent/JP6668340B2/ja active Active
- 2015-10-21 MY MYPI2017701254A patent/MY184103A/en unknown
- 2015-10-21 CN CN201580069889.1A patent/CN107109434A/zh active Pending
- 2015-10-21 SG SG11201703149RA patent/SG11201703149RA/en unknown
- 2015-10-21 CA CA2965495A patent/CA2965495C/en active Active
- 2015-10-21 MX MX2017005306A patent/MX2017005306A/es unknown
- 2015-10-21 US US14/919,300 patent/US9816110B2/en active Active
- 2015-10-21 WO PCT/US2015/056653 patent/WO2016064999A1/en not_active Ceased
- 2015-10-21 BR BR112017008022-2A patent/BR112017008022A2/pt not_active Application Discontinuation
- 2015-10-21 EA EA201790698A patent/EA037255B1/ru unknown
- 2015-10-21 AU AU2015335921A patent/AU2015335921B2/en active Active
- 2015-10-21 EP EP15797495.7A patent/EP3209785B1/en active Active
- 2015-10-22 TW TW110128495A patent/TWI780847B/zh active
- 2015-10-22 TW TW104134716A patent/TWI747808B/zh active
- 2015-10-23 AR ARP150103445A patent/AR102420A1/es unknown
-
2017
- 2017-04-09 IL IL251674A patent/IL251674B/en active IP Right Grant
- 2017-10-13 US US15/783,525 patent/US20180030480A1/en not_active Abandoned
-
2019
- 2019-09-19 US US16/575,506 patent/US11788102B2/en active Active
-
2021
- 2021-02-12 US US17/175,025 patent/US11268109B2/en active Active
-
2023
- 2023-09-06 US US18/461,798 patent/US20240018553A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US9816110B2 (en) | 2017-11-14 |
| SG11201703149RA (en) | 2018-02-27 |
| CN107109434A (zh) | 2017-08-29 |
| US20240018553A1 (en) | 2024-01-18 |
| US20210171984A1 (en) | 2021-06-10 |
| EA201790698A1 (ru) | 2018-01-31 |
| AU2015335921B2 (en) | 2020-06-25 |
| CA2965495A1 (en) | 2016-04-28 |
| TWI780847B (zh) | 2022-10-11 |
| CA2965495C (en) | 2022-05-24 |
| AU2015335921A1 (en) | 2017-05-04 |
| MY184103A (en) | 2021-03-18 |
| JP2017535258A (ja) | 2017-11-30 |
| TWI747808B (zh) | 2021-12-01 |
| TW201629229A (zh) | 2016-08-16 |
| EP3209785B1 (en) | 2019-07-31 |
| EA037255B1 (ru) | 2021-02-26 |
| TW202144576A (zh) | 2021-12-01 |
| US20160115502A1 (en) | 2016-04-28 |
| WO2016064999A1 (en) | 2016-04-28 |
| IL251674B (en) | 2020-10-29 |
| US11788102B2 (en) | 2023-10-17 |
| MX2017005306A (es) | 2018-01-09 |
| EP3209785A1 (en) | 2017-08-30 |
| JP6668340B2 (ja) | 2020-03-18 |
| US20200002732A1 (en) | 2020-01-02 |
| KR102243243B1 (ko) | 2021-04-22 |
| US20180030480A1 (en) | 2018-02-01 |
| IL251674A0 (en) | 2017-06-29 |
| US11268109B2 (en) | 2022-03-08 |
| KR20170096999A (ko) | 2017-08-25 |
| AR102420A1 (es) | 2017-03-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B12B | Appeal against refusal [chapter 12.2 patent gazette] |