EA037255B1 - Новые сайты интеграции cho и их применения - Google Patents

Новые сайты интеграции cho и их применения Download PDF

Info

Publication number
EA037255B1
EA037255B1 EA201790698A EA201790698A EA037255B1 EA 037255 B1 EA037255 B1 EA 037255B1 EA 201790698 A EA201790698 A EA 201790698A EA 201790698 A EA201790698 A EA 201790698A EA 037255 B1 EA037255 B1 EA 037255B1
Authority
EA
Eurasian Patent Office
Prior art keywords
exogenous
site
goi
nucleic acid
seq
Prior art date
Application number
EA201790698A
Other languages
English (en)
Russian (ru)
Other versions
EA201790698A1 (ru
Inventor
Ин Шень
Дарья Бураков
Ган Чень
Джэймс П. Фендл
Original Assignee
Регенерон Фармасьютикалз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA037255(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Регенерон Фармасьютикалз, Инк. filed Critical Регенерон Фармасьютикалз, Инк.
Publication of EA201790698A1 publication Critical patent/EA201790698A1/ru
Publication of EA037255B1 publication Critical patent/EA037255B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
EA201790698A 2014-10-23 2015-10-21 Новые сайты интеграции cho и их применения EA037255B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (2)

Publication Number Publication Date
EA201790698A1 EA201790698A1 (ru) 2018-01-31
EA037255B1 true EA037255B1 (ru) 2021-02-26

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201790698A EA037255B1 (ru) 2014-10-23 2015-10-21 Новые сайты интеграции cho и их применения

Country Status (16)

Country Link
US (5) US9816110B2 (enExample)
EP (1) EP3209785B1 (enExample)
JP (1) JP6668340B2 (enExample)
KR (1) KR102243243B1 (enExample)
CN (1) CN107109434B (enExample)
AR (1) AR102420A1 (enExample)
AU (1) AU2015335921B2 (enExample)
BR (1) BR112017008022A2 (enExample)
CA (1) CA2965495C (enExample)
EA (1) EA037255B1 (enExample)
IL (1) IL251674B (enExample)
MX (1) MX2017005306A (enExample)
MY (1) MY184103A (enExample)
SG (1) SG11201703149RA (enExample)
TW (2) TWI747808B (enExample)
WO (1) WO2016064999A1 (enExample)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3209785B1 (en) 2014-10-23 2019-07-31 Regeneron Pharmaceuticals, Inc. Novel cho integration sites and uses thereof
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
BR112018071283A2 (pt) 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno.
US11530277B2 (en) * 2016-04-20 2022-12-20 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
JP2020501593A (ja) * 2016-12-20 2020-01-23 ディベロップメント センター フォー バイオテクノロジーＤｅｖｅｌｏｐｍｅｎｔ Ｃｅｎｔｅｒ Ｆｏｒ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 改変細胞、調製方法、及び構築物
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
WO2018150271A1 (en) * 2017-02-17 2018-08-23 Lonza Ltd. Mammalian cells for producing adeno-associated viruses
US11959093B2 (en) 2017-03-19 2024-04-16 Applied Stemcell, Inc. Integration sites and uses thereof
KR102531749B1 (ko) * 2017-08-11 2023-05-10 베링거 인겔하임 인터내셔날 게엠베하 Cho 세포 내 통합 부위
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
WO2019126562A1 (en) * 2017-12-22 2019-06-27 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B2 (en) * 2017-12-22 2026-01-01 Genentech Inc Targeted integration of nucleic acids
KR102559149B1 (ko) 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 재조합효소 매개 카세트 교환을 이용한 다중특이적 항체 스크리닝 방법
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
EP3980544A1 (en) * 2018-12-21 2022-04-13 F. Hoffmann-La Roche AG Targeted integration of nucleic acids
SG11202110076PA (en) * 2019-04-02 2021-10-28 Chugai Pharmaceutical Co Ltd Method of introducing target-specific foreign gene
EP3990649A1 (en) 2019-06-26 2022-05-04 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
JP7887833B2 (ja) * 2020-06-02 2026-07-10 キャタレント ファーマ ソリューションズ リミテッド ライアビリティ カンパニー 遺伝子挿入用の複数のドックを有している細胞株
KR20230027043A (ko) * 2020-06-24 2023-02-27 제넨테크, 인크. 핵산의 표적화 통합
WO2022079083A1 (en) 2020-10-15 2022-04-21 F. Hoffmann-La Roche Ag Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
AR127674A1 (es) 2021-10-18 2024-02-21 Regeneron Pharma Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
US20250098652A1 (en) * 2022-01-28 2025-03-27 The Trustees Of Columbia University In The City Of New York Genetic Modifications for Xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
US20240168018A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
CN121794393A (zh) 2023-09-07 2026-04-03 里珍纳龙药品有限公司 经共价表面修饰的腺相关病毒的生产和纯化
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production
WO2026162509A1 (en) 2025-01-28 2026-08-06 Selexis Sa Integration regions and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
WO2012138887A1 (en) * 2011-04-05 2012-10-11 The Scripps Research Institute Chromosomal landing pads and related uses

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
ES2375266T3 (es) 1998-07-21 2012-02-28 Millipore Corporation Un polinucleótido que comprende un elemento de apertura de cromatina ubicuo (ucoe).
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
AU2002254529A1 (en) 2001-04-04 2002-10-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Nucleic acids for transgene expression
US20040045043A1 (en) * 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
EP3156494B8 (en) * 2008-12-04 2018-09-19 Sangamo Therapeutics, Inc. Genome editing in rats using zinc-finger nucleases
CA2833636A1 (en) 2011-04-20 2012-10-26 Amplimmune, Inc. Antibodies and other molecules that bind b7-h1 and pd-1
EP3683319A1 (en) * 2011-06-01 2020-07-22 Precision Biosciences, Inc. Methods and products for producing engineered mammalian cell lines with amplified transgenes
US9222105B2 (en) * 2011-10-27 2015-12-29 Sangamo Biosciences, Inc. Methods and compositions for modification of the HPRT locus
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
EP3209785B1 (en) 2014-10-23 2019-07-31 Regeneron Pharmaceuticals, Inc. Novel cho integration sites and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
WO2012138887A1 (en) * 2011-04-05 2012-10-11 The Scripps Research Institute Chromosomal landing pads and related uses

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHRISTINE LATTENMAYER ; MARTINA LOESCHEL ; WILLIBALD STEINFELLNER ; EVELYN TRUMMER ; DETHARDT MUELLER ; KORNELIA SCHRIEBL ; KAROLA: "Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 51, no. 3, 15 November 2006 (2006-11-15), Do, pages 171 - 182, XP019448503, ISSN: 1573-0778, DOI: 10.1007/s10616-006-9029-0 *
HONG ZHOU, ZHI-GANG LIU, ZHI-WEI SUN, YING HUANG, WEI-YUAN YU: "Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 147, no. 2, 17 May 2010 (2010-05-17), Amsterdam NL, pages 122 - 129, XP002711613, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2010.03.020 *
LEWIS N E, LIU X, LI Y, NAGARAJAN H, YERGANIAN G, O'BRIEN E, BORDBAR A, ROTH A M, ROSENBLOOM J, BIAN C, XIE M, CHEN W, LI N, BAYCI: "Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.", NATURE BIOTECHNOLOGY, vol. 31, no. 8, 1 August 2013 (2013-08-01), pages 759 - 765, XP002753785, ISSN: 1546-1696, DOI: 10.1038/nbt.2624 *
MICHEL SADELAIN, EIRINI P. PAPAPETROU, FREDERIC D. BUSHMAN: "Safe harbours for the integration of new DNA in the human genome", NATURE REVIEWS CANCER, NATURE PUB. GROUP, vol. 12, no. 1, 1 January 2011 (2011-01-01), pages 51 - 58, XP055018235, ISSN: 1474175X, DOI: 10.1038/nrc3179 *
SCOTT BAHR;LAURA CORTNER;SARA LADLEY;TRISSA BORGSCHULTE: "Evaluating the effect of chromosomal context on zinc finger nuclease efficiency", BMC PROCEEDINGS, BIOMED CENTRAL LTD, LONDON UK, vol. 7, no. Suppl 6, 4 December 2013 (2013-12-04), London UK, pages P3, XP021170326, ISSN: 1753-6561, DOI: 10.1186/1753-6561-7-S6-P3 *
XUN XU, HARISH NAGARAJAN, NATHAN E LEWIS, SHENGKAI PAN, ZHIMING CAI, XIN LIU, WENBIN CHEN, MIN XIE, WENLIANG WANG, STEPHANIE HAMMO: "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line", NATURE BIOTECHNOLOGY, GALE GROUP INC., NEW YORK, US, vol. 29, no. 8, us, pages 735 - 741, XP055223996, ISSN: 1087-0156, DOI: 10.1038/nbt.1932 *

Also Published As

Publication number Publication date
JP2017535258A (ja) 2017-11-30
CN107109434B (zh) 2026-05-26
CA2965495A1 (en) 2016-04-28
EA201790698A1 (ru) 2018-01-31
TW202144576A (zh) 2021-12-01
MY184103A (en) 2021-03-18
BR112017008022A2 (pt) 2018-02-20
IL251674A0 (en) 2017-06-29
EP3209785A1 (en) 2017-08-30
JP6668340B2 (ja) 2020-03-18
MX2017005306A (es) 2018-01-09
SG11201703149RA (en) 2018-02-27
US20180030480A1 (en) 2018-02-01
US12565667B2 (en) 2026-03-03
WO2016064999A1 (en) 2016-04-28
EP3209785B1 (en) 2019-07-31
AR102420A1 (es) 2017-03-01
US20210171984A1 (en) 2021-06-10
TWI747808B (zh) 2021-12-01
KR102243243B1 (ko) 2021-04-22
KR20170096999A (ko) 2017-08-25
IL251674B (en) 2020-10-29
US11268109B2 (en) 2022-03-08
US20200002732A1 (en) 2020-01-02
US11788102B2 (en) 2023-10-17
US9816110B2 (en) 2017-11-14
US20240018553A1 (en) 2024-01-18
AU2015335921B2 (en) 2020-06-25
TWI780847B (zh) 2022-10-11
CA2965495C (en) 2022-05-24
AU2015335921A1 (en) 2017-05-04
CN107109434A (zh) 2017-08-29
TW201629229A (zh) 2016-08-16
US20160115502A1 (en) 2016-04-28

Similar Documents

Publication Publication Date Title
US12565667B2 (en) CHO integration sites and uses thereof
US11884917B2 (en) Conditional CRISPR sgRNA expression
EP2150617B1 (en) Enhanced expression and stability regions
CN106520829B (zh) 一种终止双等位基因转录的方法
EP1815001B1 (en) Gene trap cassettes for random and targeted conditional gene inactivation
US11254928B2 (en) Gene modification assays
WO2022123242A1 (en) Cho cell modification
CN117660352A (zh) Nfat5基因定点整合宿主细胞
JP5999602B2 (ja) 遺伝子ターゲティングベクター、その作製方法及び利用方法
Schnütgen et al. High throughput gene trapping and postinsertional modifications of gene trap alleles
HK1235432A1 (en) Novel cho integration sites and uses thereof
HK1235432B (en) Novel cho integration sites and uses thereof
EP1715053A1 (en) Gene trap cassettes for random and targeted conditional gene inactivation
TW201840849A (zh) 編輯核酸序列之組成物及方法
Roy λ-integrase mediated seamless vector transgenesis platform
JP2025522793A (ja) 編集可能な細胞株
WO2013089123A1 (ja) 遺伝子ターゲティングベクター及びその利用方法