EA037255B1 - Новые сайты интеграции cho и их применения - Google Patents
Новые сайты интеграции cho и их применения Download PDFInfo
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- EA037255B1 EA037255B1 EA201790698A EA201790698A EA037255B1 EA 037255 B1 EA037255 B1 EA 037255B1 EA 201790698 A EA201790698 A EA 201790698A EA 201790698 A EA201790698 A EA 201790698A EA 037255 B1 EA037255 B1 EA 037255B1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462067774P | 2014-10-23 | 2014-10-23 | |
| PCT/US2015/056653 WO2016064999A1 (en) | 2014-10-23 | 2015-10-21 | Novel cho integration sites and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201790698A1 EA201790698A1 (ru) | 2018-01-31 |
| EA037255B1 true EA037255B1 (ru) | 2021-02-26 |
Family
ID=54602002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201790698A EA037255B1 (ru) | 2014-10-23 | 2015-10-21 | Новые сайты интеграции cho и их применения |
Country Status (16)
| Country | Link |
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| US (5) | US9816110B2 (enExample) |
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| KR102906454B1 (ko) * | 2016-04-20 | 2026-01-02 | 리제너론 파마슈티칼스 인코포레이티드 | 발현 강화 유전자좌의 사용에 기초하여 항체를 만들기 위한 조성물 및 방법 |
| EP3504328A1 (en) | 2016-08-24 | 2019-07-03 | Regeneron Pharmaceuticals, Inc. | Host cell protein modification |
| EP4130280A3 (en) * | 2016-12-20 | 2023-04-19 | Development Center for Biotechnology | Targeted integration sites in chinese hamster ovary cell genome |
| US20210309988A1 (en) * | 2017-02-07 | 2021-10-07 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| KR102719587B1 (ko) * | 2017-02-17 | 2024-10-17 | 론자 리미티드 | 아데노-관련 바이러스 생산을 위한 포유동물 세포 |
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| CN109337927B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用 |
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| JP2025503925A (ja) * | 2022-01-28 | 2025-02-06 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 異種移植のための遺伝子改変 |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
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| US20180030480A1 (en) | 2018-02-01 |
| EP3209785A1 (en) | 2017-08-30 |
| CA2965495C (en) | 2022-05-24 |
| US20240018553A1 (en) | 2024-01-18 |
| TWI747808B (zh) | 2021-12-01 |
| AU2015335921B2 (en) | 2020-06-25 |
| CN107109434A (zh) | 2017-08-29 |
| MX2017005306A (es) | 2018-01-09 |
| KR102243243B1 (ko) | 2021-04-22 |
| US20210171984A1 (en) | 2021-06-10 |
| WO2016064999A1 (en) | 2016-04-28 |
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| US20160115502A1 (en) | 2016-04-28 |
| IL251674A0 (en) | 2017-06-29 |
| CA2965495A1 (en) | 2016-04-28 |
| JP2017535258A (ja) | 2017-11-30 |
| MY184103A (en) | 2021-03-18 |
| AR102420A1 (es) | 2017-03-01 |
| SG11201703149RA (en) | 2018-02-27 |
| US11788102B2 (en) | 2023-10-17 |
| BR112017008022A2 (pt) | 2018-02-20 |
| KR20170096999A (ko) | 2017-08-25 |
| US20200002732A1 (en) | 2020-01-02 |
| EA201790698A1 (ru) | 2018-01-31 |
| AU2015335921A1 (en) | 2017-05-04 |
| EP3209785B1 (en) | 2019-07-31 |
| JP6668340B2 (ja) | 2020-03-18 |
| US11268109B2 (en) | 2022-03-08 |
| TWI780847B (zh) | 2022-10-11 |
| US9816110B2 (en) | 2017-11-14 |
| TW201629229A (zh) | 2016-08-16 |
| TW202144576A (zh) | 2021-12-01 |
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