IL251674B - New cho integration sites and their uses - Google Patents
New cho integration sites and their usesInfo
- Publication number
- IL251674B IL251674B IL251674A IL25167417A IL251674B IL 251674 B IL251674 B IL 251674B IL 251674 A IL251674 A IL 251674A IL 25167417 A IL25167417 A IL 25167417A IL 251674 B IL251674 B IL 251674B
- Authority
- IL
- Israel
- Prior art keywords
- integration sites
- novel cho
- novel
- cho integration
- sites
- Prior art date
Links
- 230000010354 integration Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462067774P | 2014-10-23 | 2014-10-23 | |
| PCT/US2015/056653 WO2016064999A1 (en) | 2014-10-23 | 2015-10-21 | Novel cho integration sites and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL251674A0 IL251674A0 (en) | 2017-06-29 |
| IL251674B true IL251674B (en) | 2020-10-29 |
Family
ID=54602002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL251674A IL251674B (en) | 2014-10-23 | 2017-04-09 | New cho integration sites and their uses |
Country Status (16)
| Country | Link |
|---|---|
| US (5) | US9816110B2 (enExample) |
| EP (1) | EP3209785B1 (enExample) |
| JP (1) | JP6668340B2 (enExample) |
| KR (1) | KR102243243B1 (enExample) |
| CN (1) | CN107109434A (enExample) |
| AR (1) | AR102420A1 (enExample) |
| AU (1) | AU2015335921B2 (enExample) |
| BR (1) | BR112017008022A2 (enExample) |
| CA (1) | CA2965495C (enExample) |
| EA (1) | EA037255B1 (enExample) |
| IL (1) | IL251674B (enExample) |
| MX (1) | MX2017005306A (enExample) |
| MY (1) | MY184103A (enExample) |
| SG (1) | SG11201703149RA (enExample) |
| TW (2) | TWI780847B (enExample) |
| WO (1) | WO2016064999A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY184103A (en) | 2014-10-23 | 2021-03-18 | Regeneron Pharma | Novel cho integration sites and uses thereof |
| US10329594B1 (en) | 2015-09-03 | 2019-06-25 | CHO Plus, Inc. | Cell lines for high level production of protein-based pharmaceuticals |
| US11649449B2 (en) | 2015-09-03 | 2023-05-16 | CHO Plus, Inc. | Hybrid cell lines for high level production of a target protein |
| WO2017184831A1 (en) | 2016-04-20 | 2017-10-26 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| KR102906454B1 (ko) * | 2016-04-20 | 2026-01-02 | 리제너론 파마슈티칼스 인코포레이티드 | 발현 강화 유전자좌의 사용에 기초하여 항체를 만들기 위한 조성물 및 방법 |
| EP3504328A1 (en) | 2016-08-24 | 2019-07-03 | Regeneron Pharmaceuticals, Inc. | Host cell protein modification |
| EP4130280A3 (en) * | 2016-12-20 | 2023-04-19 | Development Center for Biotechnology | Targeted integration sites in chinese hamster ovary cell genome |
| US20210309988A1 (en) * | 2017-02-07 | 2021-10-07 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| KR102719587B1 (ko) * | 2017-02-17 | 2024-10-17 | 론자 리미티드 | 아데노-관련 바이러스 생산을 위한 포유동물 세포 |
| WO2018175153A1 (en) * | 2017-03-19 | 2018-09-27 | Applied Stemcell, Inc. | Novel integration sites and uses thereof |
| AU2018315428B2 (en) * | 2017-08-11 | 2024-11-14 | Boehringer Ingelheim International Gmbh | Integration sites in CHO cells |
| CN107723276B (zh) * | 2017-11-02 | 2021-08-13 | 上海交通大学 | 一种稳定高表达目标产物的细胞株的构建方法和试剂盒 |
| US20200318106A1 (en) * | 2017-12-22 | 2020-10-08 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| WO2019126634A2 (en) * | 2017-12-22 | 2019-06-27 | Genentech, Inc. | Targeted integration of nucleic acids |
| WO2020084034A1 (en) | 2018-10-26 | 2020-04-30 | F. Hoffmann-La Roche Ag | Multispecific antibody screening method using recombinase mediated cassette exchange |
| CN109295093B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用 |
| CN109207432B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用 |
| CN109295092B (zh) * | 2018-10-30 | 2021-06-29 | 江南大学 | 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用 |
| CN109321604B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用 |
| CN109337927B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用 |
| CN109136193B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用 |
| SG11202106523SA (en) * | 2018-12-21 | 2021-07-29 | Genentech Inc | Targeted integration of nucleic acids |
| MX2021011903A (es) | 2019-04-02 | 2021-10-26 | Chugai Pharmaceutical Co Ltd | Método para introducir un gen extraño específico del objetivo. |
| CA3141233A1 (en) | 2019-06-26 | 2020-12-30 | Shahram Misaghi | Randomized configuration targeted integration of nucleic acids |
| EP3901266A1 (en) * | 2020-04-22 | 2021-10-27 | LEK Pharmaceuticals d.d. | Super-enhancers for recombinant gene expression in cho cells |
| WO2021247672A1 (en) * | 2020-06-02 | 2021-12-09 | Catalent Pharma Solutions, Llc | Nucleic acid constructs for protein manufacture |
| CN116096907A (zh) * | 2020-06-24 | 2023-05-09 | 基因泰克公司 | 核酸的靶向整合 |
| AR123777A1 (es) | 2020-10-15 | 2023-01-11 | Hoffmann La Roche | Constructos de ácido nucleico para transcripción de arn va |
| CN113755447B (zh) * | 2021-09-23 | 2022-04-29 | 云舟生物科技(广州)股份有限公司 | 一种用于生产腺病毒的293a细胞株及其制备与应用 |
| US12234472B2 (en) | 2021-10-18 | 2025-02-25 | Regeneron Pharmaceuticals, Inc. | Eukaryotic cells comprising adenovirus-associated virus polynucleotides |
| EP4419691A1 (en) | 2021-10-18 | 2024-08-28 | Regeneron Pharmaceuticals, Inc. | Controlled transcription of polynucleotides |
| JP2024539076A (ja) | 2021-10-18 | 2024-10-28 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 安定した組み込み部位を産生するために組み込まれたＣａｓ９遺伝子を含む哺乳動物細胞、並びに安定した組み込み部位及び他の部位を含む哺乳動物細胞 |
| JP2025503925A (ja) * | 2022-01-28 | 2025-02-06 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 異種移植のための遺伝子改変 |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
| US20240167984A1 (en) | 2022-11-18 | 2024-05-23 | Regeneron Pharmaceuticals, Inc. | Methods for detecting and determining protein structures and stability in fluids, including biological fluids |
| CN118726475B (zh) * | 2023-03-28 | 2025-07-08 | 深圳太力生物技术有限责任公司 | 核酸、重组载体、靶向整合细胞、基因表达方法和应用 |
| WO2024211287A1 (en) * | 2023-04-03 | 2024-10-10 | Seagen Inc. | Production cell lines with targeted integration sites |
| WO2025054526A1 (en) | 2023-09-07 | 2025-03-13 | Regeneron Pharmaceuticals, Inc. | Production and purification of covalently surface modified adeno-associated virus |
| TW202540433A (zh) | 2023-11-21 | 2025-10-16 | 美商再生元醫藥公司 | 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| ATE539161T1 (de) | 1998-07-21 | 2012-01-15 | Millipore Corp | Ein ein allgegenwärtiges chromatin-öffnendes element (ucoe) enthaltendes polynucleotid |
| US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| AU2002254529A1 (en) | 2001-04-04 | 2002-10-21 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of | Nucleic acids for transgene expression |
| US20040045043A1 (en) * | 2002-05-20 | 2004-03-04 | Finney Robert E. | Compositions and methods for generating conditional knockouts |
| US8673589B2 (en) | 2002-05-29 | 2014-03-18 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
| US7771997B2 (en) | 2007-06-04 | 2010-08-10 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| CN102625655B (zh) * | 2008-12-04 | 2016-07-06 | 桑格摩生物科学股份有限公司 | 使用锌指核酸酶在大鼠中进行基因组编辑 |
| SG10201914098YA (en) | 2011-04-05 | 2020-02-27 | Scripps Research Inst | Chromosomal landing pads and related uses |
| RS57324B1 (sr) | 2011-04-20 | 2018-08-31 | Medimmune Llc | Antitela i drugi molekuli koji vezuju b7-h1 i pd-1 |
| EP2714936B1 (en) * | 2011-06-01 | 2018-12-12 | Precision Biosciences, Inc. | Methods and products for producing engineered mammalian cell lines with amplified transgenes |
| JP6188703B2 (ja) * | 2011-10-27 | 2017-08-30 | サンガモ セラピューティクス， インコーポレイテッド | Ｈｐｒｔ遺伝子座を修飾するための方法および組成物 |
| JP6559063B2 (ja) | 2012-05-07 | 2019-08-14 | サンガモ セラピューティクス， インコーポレイテッド | 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物 |
| MY184103A (en) | 2014-10-23 | 2021-03-18 | Regeneron Pharma | Novel cho integration sites and uses thereof |
-
2015
- 2015-10-21 MY MYPI2017701254A patent/MY184103A/en unknown
- 2015-10-21 CN CN201580069889.1A patent/CN107109434A/zh active Pending
- 2015-10-21 KR KR1020177012465A patent/KR102243243B1/ko active Active
- 2015-10-21 JP JP2017519646A patent/JP6668340B2/ja active Active
- 2015-10-21 WO PCT/US2015/056653 patent/WO2016064999A1/en not_active Ceased
- 2015-10-21 MX MX2017005306A patent/MX2017005306A/es unknown
- 2015-10-21 AU AU2015335921A patent/AU2015335921B2/en active Active
- 2015-10-21 CA CA2965495A patent/CA2965495C/en active Active
- 2015-10-21 EA EA201790698A patent/EA037255B1/ru unknown
- 2015-10-21 EP EP15797495.7A patent/EP3209785B1/en active Active
- 2015-10-21 BR BR112017008022-2A patent/BR112017008022A2/pt not_active Application Discontinuation
- 2015-10-21 SG SG11201703149RA patent/SG11201703149RA/en unknown
- 2015-10-21 US US14/919,300 patent/US9816110B2/en active Active
- 2015-10-22 TW TW110128495A patent/TWI780847B/zh active
- 2015-10-22 TW TW104134716A patent/TWI747808B/zh active
- 2015-10-23 AR ARP150103445A patent/AR102420A1/es unknown
-
2017
- 2017-04-09 IL IL251674A patent/IL251674B/en active IP Right Grant
- 2017-10-13 US US15/783,525 patent/US20180030480A1/en not_active Abandoned
-
2019
- 2019-09-19 US US16/575,506 patent/US11788102B2/en active Active
-
2021
- 2021-02-12 US US17/175,025 patent/US11268109B2/en active Active
-
2023
- 2023-09-06 US US18/461,798 patent/US20240018553A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20180030480A1 (en) | 2018-02-01 |
| EP3209785A1 (en) | 2017-08-30 |
| CA2965495C (en) | 2022-05-24 |
| US20240018553A1 (en) | 2024-01-18 |
| TWI747808B (zh) | 2021-12-01 |
| EA037255B1 (ru) | 2021-02-26 |
| AU2015335921B2 (en) | 2020-06-25 |
| CN107109434A (zh) | 2017-08-29 |
| MX2017005306A (es) | 2018-01-09 |
| KR102243243B1 (ko) | 2021-04-22 |
| US20210171984A1 (en) | 2021-06-10 |
| WO2016064999A1 (en) | 2016-04-28 |
| US20160115502A1 (en) | 2016-04-28 |
| IL251674A0 (en) | 2017-06-29 |
| CA2965495A1 (en) | 2016-04-28 |
| JP2017535258A (ja) | 2017-11-30 |
| MY184103A (en) | 2021-03-18 |
| AR102420A1 (es) | 2017-03-01 |
| SG11201703149RA (en) | 2018-02-27 |
| US11788102B2 (en) | 2023-10-17 |
| BR112017008022A2 (pt) | 2018-02-20 |
| KR20170096999A (ko) | 2017-08-25 |
| US20200002732A1 (en) | 2020-01-02 |
| EA201790698A1 (ru) | 2018-01-31 |
| AU2015335921A1 (en) | 2017-05-04 |
| EP3209785B1 (en) | 2019-07-31 |
| JP6668340B2 (ja) | 2020-03-18 |
| US11268109B2 (en) | 2022-03-08 |
| TWI780847B (zh) | 2022-10-11 |
| US9816110B2 (en) | 2017-11-14 |
| TW201629229A (zh) | 2016-08-16 |
| TW202144576A (zh) | 2021-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL251674B (en) | New cho integration sites and their uses | |
| IL247014A0 (en) | 6-heteroaryloxy and 6-aryloxy-quinoline-2-carboxamides and their use | |
| PL3180060T3 (pl) | Urządzenie | |
| SG11201606340WA (en) | Device | |
| SG11201701129PA (en) | Electronic-component-mounting device | |
| TWI563230B (en) | Air-exchanging device | |
| SI3013201T1 (sl) | Naprava za rezanje živil | |
| SG11201606421RA (en) | Intermittent-bubbling device | |
| SG11201607462YA (en) | Device | |
| GB2525615B (en) | Adjustable shootbolt assembly | |
| GB2532862B (en) | Animal-trapping device | |
| GB201420436D0 (en) | Device | |
| GB201411628D0 (en) | Device | |
| SG11201702864QA (en) | Cell-capturing device | |
| PL3045637T3 (pl) | System okuciowy | |
| GB201419857D0 (en) | Device | |
| PT3092081T (pt) | Dispositivo de aplicação | |
| LU92619B1 (en) | Sinterstrand-charging device | |
| GB2534344B (en) | Frenotomy device | |
| GB201420463D0 (en) | Griptape letters | |
| HU4496U (en) | Compactors | |
| PL2899341T3 (pl) | System okuciowy | |
| AU358007S (en) | Extrusion | |
| GB201416454D0 (en) | Device | |
| GB201416453D0 (en) | Device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |