TWI623549B - 肉毒桿菌毒素製造方法 - Google Patents
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- TWI623549B TWI623549B TW105139480A TW105139480A TWI623549B TW I623549 B TWI623549 B TW I623549B TW 105139480 A TW105139480 A TW 105139480A TW 105139480 A TW105139480 A TW 105139480A TW I623549 B TWI623549 B TW I623549B
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- botulinum toxin
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
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- B01D—SEPARATION
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
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- B01D61/14—Ultrafiltration; Microfiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
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- B01D61/58—Multistep processes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
- B01D2315/16—Diafiltration
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- Health & Medical Sciences (AREA)
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- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| KR10-2015-0168196 | 2015-11-30 | ||
| KR1020150168196A KR101775682B1 (ko) | 2015-11-30 | 2015-11-30 | 보툴리눔 독소의 제조방법 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| JP5951490B2 (ja) * | 2009-10-21 | 2016-07-13 | ルバンス セラピュティックス インク．Ｒｅｖａｎｃｅ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 非複合ボツリヌス神経毒素を精製するための方法およびシステム |
| KR101723168B1 (ko) * | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR102463881B1 (ko) * | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
| KR102516203B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 제조방법 |
| KR102516204B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 정제방법 |
| EP3962924A1 (en) * | 2019-05-03 | 2022-03-09 | Genentech, Inc. | Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform |
| KR102287437B1 (ko) * | 2019-08-14 | 2021-08-09 | 주식회사 프로톡스 | 보툴리눔 독소의 제조방법 |
| TW202136286A (zh) * | 2019-12-20 | 2021-10-01 | 瑞士商葛德瑪控股公司 | 純化肉毒桿菌毒素的方法 |
| CN112480221A (zh) * | 2020-12-02 | 2021-03-12 | 湖北省农业科学院畜牧兽医研究所 | 产气荚膜梭菌毒素抗原浓缩工艺 |
| KR102724651B1 (ko) * | 2021-07-08 | 2024-11-01 | 주식회사 파마리서치바이오 | 비-독소 단백질이 제거된 클로스트리디움 보툴리눔 신경독소 단백질의 정제방법 |
| CO2023018742A1 (es) * | 2023-12-29 | 2025-07-07 | Lifefactors S A S | Proceso de purificación de macromoléculas con etapas de precipitación selectiva, concentración y diálisis combinadas en condiciones constantes de estabilidad con el cual se obtienen formulaciones estables y de alta pureza |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060228780A1 (en) * | 2003-09-25 | 2006-10-12 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
| CN102089438A (zh) * | 2008-05-19 | 2011-06-08 | 玫帝托克斯股份有限公司 | 使用含有植物源性组分的培养基和挠性密闭容器来制备肉毒梭菌毒素的方法 |
| US20150337281A1 (en) * | 2013-08-02 | 2015-11-26 | Daewoong Co., Ltd. | Method for production of botulinum toxin |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH06192296A (ja) | 1992-10-28 | 1994-07-12 | Chiba Pref Gov | 治療用医薬品としての結晶ａ型ボツリヌス毒素の製造法。 |
| JPH06199296A (ja) | 1992-12-29 | 1994-07-19 | Toyota Motor Corp | ガスタービンエンジンを推進力とする水上飛行機 |
| DE19925739A1 (de) * | 1999-06-07 | 2000-12-21 | Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh | Therapeutikum mit einem Botulinum-Neurotoxin |
| GB9914743D0 (en) * | 1999-06-24 | 1999-08-25 | Knoll Ag | Therapeutic agents |
| KR20030060150A (ko) * | 2002-01-07 | 2003-07-16 | (주)메디톡스 | 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법 |
| CN1930186A (zh) * | 2005-03-03 | 2007-03-14 | 阿勒根公司 | 为获得梭菌毒素的梭菌细菌培养基及方法 |
| KR101025617B1 (ko) | 2008-02-25 | 2011-03-30 | 메덱스젠 주식회사 | 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법 |
| US8129139B2 (en) * | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| JP2011074025A (ja) * | 2009-09-30 | 2011-04-14 | Chemo-Sero-Therapeutic Research Inst | ボツリヌス毒素の精製方法 |
| JP5951490B2 (ja) | 2009-10-21 | 2016-07-13 | ルバンス セラピュティックス インク．Ｒｅｖａｎｃｅ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 非複合ボツリヌス神経毒素を精製するための方法およびシステム |
| KR101134146B1 (ko) * | 2010-05-31 | 2012-04-19 | 메덱스젠 주식회사 | 국소 근마비 효과를 갖는 비확산형 보툴리눔 독소와 그의 정제방법 |
| UY33452A (es) | 2010-06-16 | 2012-01-31 | Bayer Schering Pharma Ag | Triazolopiridinas sustituidas |
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2015
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2016
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060228780A1 (en) * | 2003-09-25 | 2006-10-12 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
| CN102089438A (zh) * | 2008-05-19 | 2011-06-08 | 玫帝托克斯股份有限公司 | 使用含有植物源性组分的培养基和挠性密闭容器来制备肉毒梭菌毒素的方法 |
| US20150337281A1 (en) * | 2013-08-02 | 2015-11-26 | Daewoong Co., Ltd. | Method for production of botulinum toxin |
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| CA2997698C (en) | 2021-04-20 |
| AU2019201315B2 (en) | 2020-10-15 |
| TW201726703A (zh) | 2017-08-01 |
| CN108350043A (zh) | 2018-07-31 |
| AU2016364597B2 (en) | 2019-01-17 |
| MX2018004650A (es) | 2018-08-15 |
| BR112018007679A2 (pt) | 2018-11-06 |
| US10619143B2 (en) | 2020-04-14 |
| RU2707255C1 (ru) | 2019-11-25 |
| KR101775682B1 (ko) | 2017-09-06 |
| JP6574528B2 (ja) | 2019-09-11 |
| WO2017095062A1 (en) | 2017-06-08 |
| US20180251741A1 (en) | 2018-09-06 |
| AU2019201315A1 (en) | 2019-03-21 |
| CA2997698A1 (en) | 2017-06-08 |
| KR20170062694A (ko) | 2017-06-08 |
| AR107218A1 (es) | 2018-04-11 |
| AU2016364597A1 (en) | 2018-04-12 |
| EP3383889A1 (en) | 2018-10-10 |
| EP3383889A4 (en) | 2019-07-17 |
| CN108350043B (zh) | 2022-06-14 |
| JP2018533977A (ja) | 2018-11-22 |
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