MX2018004650A - Metodo para producir toxina botulinica. - Google Patents
Metodo para producir toxina botulinica.Info
- Publication number
- MX2018004650A MX2018004650A MX2018004650A MX2018004650A MX2018004650A MX 2018004650 A MX2018004650 A MX 2018004650A MX 2018004650 A MX2018004650 A MX 2018004650A MX 2018004650 A MX2018004650 A MX 2018004650A MX 2018004650 A MX2018004650 A MX 2018004650A
- Authority
- MX
- Mexico
- Prior art keywords
- botulinum toxin
- precipitate
- buffer
- ammonium sulfate
- hydrochloric acid
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/58—Multistep processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
- B01D2315/16—Diafiltration
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método para producir una toxina botulínica que comprende: (a) tratar un cultivo de una cepa productora de toxina botulínica con ácido para formar un precipitado que contiene toxina botulínica; (b) añadir un tampón al precipitado que contiene toxina botulínica del paso (a), seguido de clarificación por al menos un método seleccionado del grupo que consiste en filtración en profundidad (DF), microfiltración (MF), ultrafiltración (UF), estéril filtración, cromatografía de membrana (MC) y centrifugación; (c) someter la solución que contiene toxina botulínica de la etapa (b) a diafiltración de UF, precipitación con sulfato de amonio o precipitación con ácido clorhídrico y luego diluir un retenido resultante de la diafiltración de UF en un tampón o disolver un precipitado resultante de la precipitación con sulfato de amonio o precipitado con ácido clorhídrico en un tampón; y (d) someter la disolución de producto retenido, la solución de precipitado con sulfato de amonio o la solución de precipitado con ácido clorhídrico de la etapa (c) a una cromatografía de intercambio aniónico (AEX) para purificar la toxina botulínica.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020150168196A KR101775682B1 (ko) | 2015-11-30 | 2015-11-30 | 보툴리눔 독소의 제조방법 |
PCT/KR2016/013506 WO2017095062A1 (en) | 2015-11-30 | 2016-11-23 | Method for producing botulinum toxin |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018004650A true MX2018004650A (es) | 2018-08-15 |
Family
ID=58797138
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018004650A MX2018004650A (es) | 2015-11-30 | 2016-11-23 | Metodo para producir toxina botulinica. |
Country Status (13)
Country | Link |
---|---|
US (1) | US10619143B2 (es) |
EP (1) | EP3383889A4 (es) |
JP (1) | JP6574528B2 (es) |
KR (1) | KR101775682B1 (es) |
CN (1) | CN108350043B (es) |
AR (1) | AR107218A1 (es) |
AU (2) | AU2016364597B2 (es) |
BR (1) | BR112018007679A2 (es) |
CA (1) | CA2997698C (es) |
MX (1) | MX2018004650A (es) |
RU (1) | RU2707255C1 (es) |
TW (1) | TWI623549B (es) |
WO (1) | WO2017095062A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT2490986T (pt) * | 2009-10-21 | 2018-11-13 | Revance Therapeutics Inc | Métodos e sistemas para purificar neurotoxina botulínica não complexada |
KR101723168B1 (ko) * | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
KR102463881B1 (ko) * | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
KR102516204B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 정제방법 |
KR102516203B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 제조방법 |
KR102287437B1 (ko) * | 2019-08-14 | 2021-08-09 | 주식회사 프로톡스 | 보툴리눔 독소의 제조방법 |
CN112480221A (zh) * | 2020-12-02 | 2021-03-12 | 湖北省农业科学院畜牧兽医研究所 | 产气荚膜梭菌毒素抗原浓缩工艺 |
KR20230009178A (ko) * | 2021-07-08 | 2023-01-17 | 주식회사 파마리서치바이오 | 비-독소 단백질이 제거된 클로스트리디움 보툴리눔 신경독소 단백질의 정제방법 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06192296A (ja) | 1992-10-28 | 1994-07-12 | Chiba Pref Gov | 治療用医薬品としての結晶a型ボツリヌス毒素の製造法。 |
JPH06199296A (ja) | 1992-12-29 | 1994-07-19 | Toyota Motor Corp | ガスタービンエンジンを推進力とする水上飛行機 |
DE19925739A1 (de) * | 1999-06-07 | 2000-12-21 | Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh | Therapeutikum mit einem Botulinum-Neurotoxin |
GB9914743D0 (en) | 1999-06-24 | 1999-08-25 | Knoll Ag | Therapeutic agents |
US7354740B2 (en) | 2003-09-25 | 2008-04-08 | Allergan, Inc. | Animal product free system and process for purifying a botulinum toxin |
US7452697B2 (en) * | 2003-09-25 | 2008-11-18 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
KR101025617B1 (ko) | 2008-02-25 | 2011-03-30 | 메덱스젠 주식회사 | 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법 |
KR20090120222A (ko) * | 2008-05-19 | 2009-11-24 | (주)메디톡스 | 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법 |
US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
JP2011074025A (ja) * | 2009-09-30 | 2011-04-14 | Chemo-Sero-Therapeutic Research Inst | ボツリヌス毒素の精製方法 |
PT2490986T (pt) | 2009-10-21 | 2018-11-13 | Revance Therapeutics Inc | Métodos e sistemas para purificar neurotoxina botulínica não complexada |
KR101134146B1 (ko) | 2010-05-31 | 2012-04-19 | 메덱스젠 주식회사 | 국소 근마비 효과를 갖는 비확산형 보툴리눔 독소와 그의 정제방법 |
UY33452A (es) | 2010-06-16 | 2012-01-31 | Bayer Schering Pharma Ag | Triazolopiridinas sustituidas |
KR101339349B1 (ko) | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
-
2015
- 2015-11-30 KR KR1020150168196A patent/KR101775682B1/ko not_active Application Discontinuation
-
2016
- 2016-11-23 RU RU2018113925A patent/RU2707255C1/ru active
- 2016-11-23 JP JP2018540360A patent/JP6574528B2/ja active Active
- 2016-11-23 AU AU2016364597A patent/AU2016364597B2/en active Active
- 2016-11-23 CA CA2997698A patent/CA2997698C/en active Active
- 2016-11-23 EP EP16870958.2A patent/EP3383889A4/en active Pending
- 2016-11-23 WO PCT/KR2016/013506 patent/WO2017095062A1/en active Application Filing
- 2016-11-23 CN CN201680061642.XA patent/CN108350043B/zh active Active
- 2016-11-23 US US15/758,042 patent/US10619143B2/en active Active
- 2016-11-23 BR BR112018007679A patent/BR112018007679A2/pt active Search and Examination
- 2016-11-23 MX MX2018004650A patent/MX2018004650A/es unknown
- 2016-11-29 AR ARP160103642A patent/AR107218A1/es unknown
- 2016-11-30 TW TW105139480A patent/TWI623549B/zh active
-
2019
- 2019-02-26 AU AU2019201315A patent/AU2019201315B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
KR101775682B1 (ko) | 2017-09-06 |
CN108350043A (zh) | 2018-07-31 |
EP3383889A1 (en) | 2018-10-10 |
EP3383889A4 (en) | 2019-07-17 |
AU2019201315B2 (en) | 2020-10-15 |
AU2016364597A1 (en) | 2018-04-12 |
KR20170062694A (ko) | 2017-06-08 |
RU2707255C1 (ru) | 2019-11-25 |
BR112018007679A2 (pt) | 2018-11-06 |
AU2019201315A1 (en) | 2019-03-21 |
CN108350043B (zh) | 2022-06-14 |
CA2997698C (en) | 2021-04-20 |
US20180251741A1 (en) | 2018-09-06 |
TW201726703A (zh) | 2017-08-01 |
CA2997698A1 (en) | 2017-06-08 |
AR107218A1 (es) | 2018-04-11 |
JP6574528B2 (ja) | 2019-09-11 |
US10619143B2 (en) | 2020-04-14 |
JP2018533977A (ja) | 2018-11-22 |
AU2016364597B2 (en) | 2019-01-17 |
WO2017095062A1 (en) | 2017-06-08 |
TWI623549B (zh) | 2018-05-11 |
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