MX2021012522A - Metodo de purificacion de la toxina botulinica. - Google Patents

Metodo de purificacion de la toxina botulinica.

Info

Publication number
MX2021012522A
MX2021012522A MX2021012522A MX2021012522A MX2021012522A MX 2021012522 A MX2021012522 A MX 2021012522A MX 2021012522 A MX2021012522 A MX 2021012522A MX 2021012522 A MX2021012522 A MX 2021012522A MX 2021012522 A MX2021012522 A MX 2021012522A
Authority
MX
Mexico
Prior art keywords
botulinum toxin
purifying
exchange chromatography
present
cation exchange
Prior art date
Application number
MX2021012522A
Other languages
English (en)
Inventor
Jae Young Kim
Jeong Sun Nam
Seungho Kim
Bum Jin Yun
Seung Kwan Choi
Jin Hee Choi
Original Assignee
Jetema Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jetema Co Ltd filed Critical Jetema Co Ltd
Publication of MX2021012522A publication Critical patent/MX2021012522A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describe un método de purificación de una toxina botulínica que incluye (a) pretratar una solución de cultivo que contiene una toxina botulínica, (b) purificar la toxina botulínica pretratada mediante el uso de cromatografía de intercambio aniónico y (c) purificar la toxina botulínica mediante el uso de cromatografía de intercambio catiónico. El método es capaz de purificar una toxina botulínica con alta pureza y actividad mediante el uso de un proceso simple que incluye cromatografía de intercambio aniónico y cromatografía de intercambio catiónico, y por tanto es útil para la producción de toxina botulínica.
MX2021012522A 2019-04-15 2020-04-14 Metodo de purificacion de la toxina botulinica. MX2021012522A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20190043868 2019-04-15
PCT/KR2020/005041 WO2020213928A1 (ko) 2019-04-15 2020-04-14 보툴리눔 독소의 정제방법

Publications (1)

Publication Number Publication Date
MX2021012522A true MX2021012522A (es) 2022-01-24

Family

ID=72837488

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021012522A MX2021012522A (es) 2019-04-15 2020-04-14 Metodo de purificacion de la toxina botulinica.

Country Status (8)

Country Link
US (1) US20220186201A1 (es)
EP (1) EP3957647A4 (es)
KR (2) KR102485146B1 (es)
CN (1) CN114341167A (es)
BR (1) BR112021020486A2 (es)
MX (1) MX2021012522A (es)
TW (1) TWI797442B (es)
WO (1) WO2020213928A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102512757B1 (ko) 2021-07-22 2023-03-22 (주)이니바이오 정제 수율이 향상된 보툴리눔 독소 복합체 정제방법
WO2024101624A1 (ko) * 2022-11-08 2024-05-16 (주)제테마 보툴리눔 독소의 정제방법

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI241195B (en) 2000-04-10 2005-10-11 Shionogi & Co Preventive agent for bile acidic diarrhea
JP2003009897A (ja) 2001-07-03 2003-01-14 Keiji Oguma ボツリヌス毒素の分離・精製法
US7452697B2 (en) 2003-09-25 2008-11-18 Allergan, Inc. Chromatographic method and system for purifying a botulinum toxin
WO2006042542A2 (en) * 2004-10-19 2006-04-27 Statens Serum Institut Production of tetanus, diphtheria, and pertussis toxins and toxoids using fermentation media containing no components of animal or soy origin
JP5006803B2 (ja) * 2005-03-03 2012-08-22 アラーガン、インコーポレイテッド クロストリジウム・バクテリアの培地およびクロストリジウム毒素を得るための方法
KR20090120222A (ko) * 2008-05-19 2009-11-24 (주)메디톡스 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법
US8440204B2 (en) * 2009-04-30 2013-05-14 Wisconsin Alumni Research Foundation Subtype of Closteridium botulinum neurotoxin type A and uses thereof
US8129139B2 (en) * 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
JP2011074025A (ja) * 2009-09-30 2011-04-14 Chemo-Sero-Therapeutic Research Inst ボツリヌス毒素の精製方法
PT2490986T (pt) * 2009-10-21 2018-11-13 Revance Therapeutics Inc Métodos e sistemas para purificar neurotoxina botulínica não complexada
KR20130092024A (ko) 2012-02-09 2013-08-20 권지성 멜로디 유아용 소변기
KR101339349B1 (ko) * 2013-08-02 2013-12-09 주식회사 대웅 보툴리눔 독소의 제조방법
GB201407525D0 (en) 2014-04-29 2014-06-11 Syntaxin Ltd Manufacture of recombinant clostridium botulinum neurotoxins
KR101775682B1 (ko) * 2015-11-30 2017-09-06 주식회사 대웅 보툴리눔 독소의 제조방법
KR102463881B1 (ko) * 2016-10-04 2022-11-07 (주)메디톡스 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법

Also Published As

Publication number Publication date
KR20200121245A (ko) 2020-10-23
KR20200121247A (ko) 2020-10-23
KR102485146B1 (ko) 2023-01-06
CN114341167A (zh) 2022-04-12
WO2020213928A1 (ko) 2020-10-22
KR102516204B1 (ko) 2023-03-30
TWI797442B (zh) 2023-04-01
US20220186201A1 (en) 2022-06-16
EP3957647A1 (en) 2022-02-23
TW202104243A (zh) 2021-02-01
BR112021020486A2 (pt) 2021-12-14
EP3957647A4 (en) 2023-01-18

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