MX2017000772A - Metodo para extraer proteinas solubles de biomasa de microalgas. - Google Patents

Metodo para extraer proteinas solubles de biomasa de microalgas.

Info

Publication number
MX2017000772A
MX2017000772A MX2017000772A MX2017000772A MX2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A MX 2017000772 A MX2017000772 A MX 2017000772A
Authority
MX
Mexico
Prior art keywords
biomass
soluble
protein isolate
soluble fraction
kda
Prior art date
Application number
MX2017000772A
Other languages
English (en)
Inventor
Patinier Samuel
Original Assignee
Roquette Freres
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roquette Freres filed Critical Roquette Freres
Publication of MX2017000772A publication Critical patent/MX2017000772A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/009—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Water Supply & Treatment (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención se refiere a un método para preparar un aislado de proteínas a partir de la biomasa de microalgas del género Chlorella, caracterizado por que comprende las siguientes etapas: suministro de una biomasa de microalgas producida por fermentación; opcionalmente, lavado de la biomasa de manera que se eliminen los compuestos solubles intersticiales, permeabilización térmica de la biomasa a una temperatura de entre 50 y 150 °C, preferentemente de entre 100 y 150 °C, durante entre 10 segundos y 5 minutos, preferentemente entre 10 segundos y 1 minuto; eliminación de la biomasa permeabilizada de esta manera mediante una técnica de separación sólido-líquido seleccionada a partir del grupo constituido por filtración frontal o tangencial, floculación y centrifugación, más específicamente centrifugación multietapa, con el fin de obtener una fracción soluble; opcionalmente, recuperación y clarificación de la fracción soluble obtenida de este modo mediante microfiltración de tal manera que se eliminen los elementos insolubles residuales de ella; ultrafiltración de la fracción soluble con una membrana con un umbral de corte inferior a 5 kDa, preferentemente de entre 1 y 5 kDa, con el fin de producir un aislado de proteínas solubles; a continuación, evaporación, pasteurización y atomización de dicho aislado de proteínas.
MX2017000772A 2014-07-18 2015-07-16 Metodo para extraer proteinas solubles de biomasa de microalgas. MX2017000772A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1456944 2014-07-18
PCT/FR2015/051940 WO2016009145A1 (fr) 2014-07-18 2015-07-16 Procede d'extraction des proteines solubles de biomasses de microalgues

Publications (1)

Publication Number Publication Date
MX2017000772A true MX2017000772A (es) 2017-04-27

Family

ID=53938354

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017000772A MX2017000772A (es) 2014-07-18 2015-07-16 Metodo para extraer proteinas solubles de biomasa de microalgas.

Country Status (8)

Country Link
US (1) US20170152294A1 (es)
EP (1) EP3169695B1 (es)
JP (1) JP2017521451A (es)
KR (1) KR20170028353A (es)
CN (1) CN106488927A (es)
BR (1) BR112017001039A2 (es)
MX (1) MX2017000772A (es)
WO (1) WO2016009145A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3169696B1 (fr) 2014-07-18 2019-06-12 Corbion Biotech, Inc. Procede d'extraction des proteines solubles de biomasses de microalgues
FR3031987B1 (fr) * 2015-01-26 2019-05-24 Corbion Biotech, Inc. Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines
WO2020225336A1 (en) 2019-05-07 2020-11-12 Roquette Freres Novel polysaccharide-based hydrogel scaffolds for wound care
CN111018069A (zh) * 2019-12-09 2020-04-17 河南大禹水处理有限公司 一种回收微生物菌体蛋白的方法
WO2023198689A1 (fr) * 2022-04-11 2023-10-19 Algama Procédé d'obtention d'un extrait protéique texturant à partir de microalgue

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1138073A1 (ru) * 1976-12-02 1985-02-07 Всесоюзный Научно-Исследовательский Биотехнический Институт Способ разрушени клеточной оболочки хлореллы
JPH01180832A (ja) * 1988-01-05 1989-07-18 Sanwa Kagaku Kenkyusho Co Ltd 高ヨウ素含有淡水クロレラからの熱水抽出物、その製法、およびその用途
JP3143636B2 (ja) 1991-09-11 2001-03-07 株式会社サン・クロレラ 細胞破裂によるクロレラ細胞壁の破砕方法
JP2000106826A (ja) * 1998-10-01 2000-04-18 Senmi Extract Kk クロレラペプチド含有組成物
CN1259335C (zh) * 2004-03-27 2006-06-14 中国科学院水生生物研究所 一种水华蓝藻制备藻蓝蛋白的方法
KR100657637B1 (ko) * 2005-03-08 2006-12-19 진현진 클로렐라로부터 액상 추출물을 추출하는 방법

Also Published As

Publication number Publication date
CN106488927A (zh) 2017-03-08
JP2017521451A (ja) 2017-08-03
BR112017001039A2 (pt) 2017-11-14
EP3169695B1 (fr) 2019-11-13
WO2016009145A1 (fr) 2016-01-21
KR20170028353A (ko) 2017-03-13
EP3169695A1 (fr) 2017-05-24
US20170152294A1 (en) 2017-06-01

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