KR101775682B1 - 보툴리눔 독소의 제조방법 - Google Patents

보툴리눔 독소의 제조방법 Download PDF

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Publication number
KR101775682B1
KR101775682B1 KR1020150168196A KR20150168196A KR101775682B1 KR 101775682 B1 KR101775682 B1 KR 101775682B1 KR 1020150168196 A KR1020150168196 A KR 1020150168196A KR 20150168196 A KR20150168196 A KR 20150168196A KR 101775682 B1 KR101775682 B1 KR 101775682B1
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botulinum toxin
exchange chromatography
anion exchange
precipitate
buffer solution
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KR20170062694A (ko
Inventor
김경윤
김청세
김명섭
설혜영
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주식회사 대웅
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Application filed by 주식회사 대웅 filed Critical 주식회사 대웅
Priority to BR112018007679A priority patent/BR112018007679A2/pt
Priority to PCT/KR2016/013506 priority patent/WO2017095062A1/en
Priority to CA2997698A priority patent/CA2997698C/en
Priority to MX2018004650A priority patent/MX2018004650A/es
Priority to RU2018113925A priority patent/RU2707255C1/ru
Priority to US15/758,042 priority patent/US10619143B2/en
Priority to JP2018540360A priority patent/JP6574528B2/ja
Priority to CN201680061642.XA priority patent/CN108350043B/zh
Priority to AU2016364597A priority patent/AU2016364597B2/en
Priority to EP16870958.2A priority patent/EP3383889A4/en
Priority to ARP160103642A priority patent/AR107218A1/es
Priority to TW105139480A priority patent/TWI623549B/zh
Publication of KR20170062694A publication Critical patent/KR20170062694A/ko
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Publication of KR101775682B1 publication Critical patent/KR101775682B1/ko
Priority to AU2019201315A priority patent/AU2019201315B2/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/58—Multistep processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020150168196A 2015-11-30 2015-11-30 보툴리눔 독소의 제조방법 Active KR101775682B1 (ko)

Priority Applications (14)

Application Number Priority Date Filing Date Title
KR1020150168196A KR101775682B1 (ko) 2015-11-30 2015-11-30 보툴리눔 독소의 제조방법
EP16870958.2A EP3383889A4 (en) 2015-11-30 2016-11-23 PROCESS FOR THE PREPARATION OF BOTULINUM TOXIN
CA2997698A CA2997698C (en) 2015-11-30 2016-11-23 Method for producing botulinum toxin
MX2018004650A MX2018004650A (es) 2015-11-30 2016-11-23 Metodo para producir toxina botulinica.
RU2018113925A RU2707255C1 (ru) 2015-11-30 2016-11-23 Способ получения ботулотоксина
US15/758,042 US10619143B2 (en) 2015-11-30 2016-11-23 Method and producing botulinum toxin
JP2018540360A JP6574528B2 (ja) 2015-11-30 2016-11-23 ボツリヌス毒素の製造方法
CN201680061642.XA CN108350043B (zh) 2015-11-30 2016-11-23 用于产生肉毒杆菌毒素的方法
BR112018007679A BR112018007679A2 (pt) 2015-11-30 2016-11-23 método para produzir uma toxina botulínica
PCT/KR2016/013506 WO2017095062A1 (en) 2015-11-30 2016-11-23 Method for producing botulinum toxin
AU2016364597A AU2016364597B2 (en) 2015-11-30 2016-11-23 Method for producing botulinum toxin
ARP160103642A AR107218A1 (es) 2015-11-30 2016-11-29 Método para producir toxina botulínica
TW105139480A TWI623549B (zh) 2015-11-30 2016-11-30 肉毒桿菌毒素製造方法
AU2019201315A AU2019201315B2 (en) 2015-11-30 2019-02-26 Method for producing botulinum toxin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150168196A KR101775682B1 (ko) 2015-11-30 2015-11-30 보툴리눔 독소의 제조방법

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KR20170062694A KR20170062694A (ko) 2017-06-08
KR101775682B1 true KR101775682B1 (ko) 2017-09-06

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US (1) US10619143B2 (enExample)
EP (1) EP3383889A4 (enExample)
JP (1) JP6574528B2 (enExample)
KR (1) KR101775682B1 (enExample)
CN (1) CN108350043B (enExample)
AR (1) AR107218A1 (enExample)
AU (2) AU2016364597B2 (enExample)
BR (1) BR112018007679A2 (enExample)
CA (1) CA2997698C (enExample)
MX (1) MX2018004650A (enExample)
RU (1) RU2707255C1 (enExample)
TW (1) TWI623549B (enExample)
WO (1) WO2017095062A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
JP5951490B2 (ja) * 2009-10-21 2016-07-13 ルバンス セラピュティックス インク．Ｒｅｖａｎｃｅ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 非複合ボツリヌス神経毒素を精製するための方法およびシステム
KR101723168B1 (ko) * 2015-04-28 2017-04-05 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물
KR102463881B1 (ko) * 2016-10-04 2022-11-07 (주)메디톡스 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법
KR102516203B1 (ko) * 2019-04-15 2023-03-30 (주)제테마 보툴리눔 독소의 제조방법
KR102516204B1 (ko) * 2019-04-15 2023-03-30 (주)제테마 보툴리눔 독소의 정제방법
EP3962924A1 (en) * 2019-05-03 2022-03-09 Genentech, Inc. Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform
KR102287437B1 (ko) * 2019-08-14 2021-08-09 주식회사 프로톡스 보툴리눔 독소의 제조방법
TW202136286A (zh) * 2019-12-20 2021-10-01 瑞士商葛德瑪控股公司 純化肉毒桿菌毒素的方法
CN112480221A (zh) * 2020-12-02 2021-03-12 湖北省农业科学院畜牧兽医研究所 产气荚膜梭菌毒素抗原浓缩工艺
KR102724651B1 (ko) * 2021-07-08 2024-11-01 주식회사 파마리서치바이오 비-독소 단백질이 제거된 클로스트리디움 보툴리눔 신경독소 단백질의 정제방법
CO2023018742A1 (es) * 2023-12-29 2025-07-07 Lifefactors S A S Proceso de purificación de macromoléculas con etapas de precipitación selectiva, concentración y diálisis combinadas en condiciones constantes de estabilidad con el cual se obtienen formulaciones estables y de alta pureza

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060228780A1 (en) * 2003-09-25 2006-10-12 Allergan, Inc. Chromatographic method and system for purifying a botulinum toxin
JP2011074025A (ja) * 2009-09-30 2011-04-14 Chemo-Sero-Therapeutic Research Inst ボツリヌス毒素の精製方法
US20120245324A1 (en) * 2009-07-13 2012-09-27 Allergan, Inc. Process for obtaining botulinum neurotoxin
KR101339349B1 (ko) * 2013-08-02 2013-12-09 주식회사 대웅 보툴리눔 독소의 제조방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06192296A (ja) 1992-10-28 1994-07-12 Chiba Pref Gov 治療用医薬品としての結晶ａ型ボツリヌス毒素の製造法。
JPH06199296A (ja) 1992-12-29 1994-07-19 Toyota Motor Corp ガスタービンエンジンを推進力とする水上飛行機
DE19925739A1 (de) * 1999-06-07 2000-12-21 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Therapeutikum mit einem Botulinum-Neurotoxin
GB9914743D0 (en) * 1999-06-24 1999-08-25 Knoll Ag Therapeutic agents
KR20030060150A (ko) * 2002-01-07 2003-07-16 (주)메디톡스 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법
CN1930186A (zh) * 2005-03-03 2007-03-14 阿勒根公司 为获得梭菌毒素的梭菌细菌培养基及方法
KR101025617B1 (ko) 2008-02-25 2011-03-30 메덱스젠 주식회사 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법
KR20090120222A (ko) * 2008-05-19 2009-11-24 (주)메디톡스 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법
JP5951490B2 (ja) 2009-10-21 2016-07-13 ルバンス セラピュティックス インク．Ｒｅｖａｎｃｅ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 非複合ボツリヌス神経毒素を精製するための方法およびシステム
KR101134146B1 (ko) * 2010-05-31 2012-04-19 메덱스젠 주식회사 국소 근마비 효과를 갖는 비확산형 보툴리눔 독소와 그의 정제방법
UY33452A (es) 2010-06-16 2012-01-31 Bayer Schering Pharma Ag Triazolopiridinas sustituidas

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060228780A1 (en) * 2003-09-25 2006-10-12 Allergan, Inc. Chromatographic method and system for purifying a botulinum toxin
US20120245324A1 (en) * 2009-07-13 2012-09-27 Allergan, Inc. Process for obtaining botulinum neurotoxin
JP2011074025A (ja) * 2009-09-30 2011-04-14 Chemo-Sero-Therapeutic Research Inst ボツリヌス毒素の精製方法
KR101339349B1 (ko) * 2013-08-02 2013-12-09 주식회사 대웅 보툴리눔 독소의 제조방법

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CA2997698C (en) 2021-04-20
AU2019201315B2 (en) 2020-10-15
TW201726703A (zh) 2017-08-01
CN108350043A (zh) 2018-07-31
TWI623549B (zh) 2018-05-11
AU2016364597B2 (en) 2019-01-17
MX2018004650A (es) 2018-08-15
BR112018007679A2 (pt) 2018-11-06
US10619143B2 (en) 2020-04-14
RU2707255C1 (ru) 2019-11-25
JP6574528B2 (ja) 2019-09-11
WO2017095062A1 (en) 2017-06-08
US20180251741A1 (en) 2018-09-06
AU2019201315A1 (en) 2019-03-21
CA2997698A1 (en) 2017-06-08
KR20170062694A (ko) 2017-06-08
AR107218A1 (es) 2018-04-11
AU2016364597A1 (en) 2018-04-12
EP3383889A1 (en) 2018-10-10
EP3383889A4 (en) 2019-07-17
CN108350043B (zh) 2022-06-14
JP2018533977A (ja) 2018-11-22

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