TWI410491B - 眼睛與中樞神經系統之細菌、黴菌、寄生蟲及病毒感染之新穎同步檢測及鑑別方法 - Google Patents
眼睛與中樞神經系統之細菌、黴菌、寄生蟲及病毒感染之新穎同步檢測及鑑別方法 Download PDFInfo
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| FR2661474B1 (fr) | 1990-04-25 | 1992-06-12 | Valeo | Double volant amortisseur de torsion pour moteur a combustion interne. |
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| CA2689306C (en) * | 2007-06-01 | 2019-01-15 | Council Of Scientific & Industrial Research | A novel method for simultaneous detection and discrimination of bacterial, fungal, parasitic and viral infections of eye and central nervous system |
| US20120040349A1 (en) * | 2009-05-12 | 2012-02-16 | Piia Von Lode | Method for detecting nucleic acids |
| EP2270202A1 (en) * | 2009-07-03 | 2011-01-05 | John Ikonomopoulos | Mycobacterial detection |
| EP3882633A1 (en) | 2012-02-09 | 2021-09-22 | MeMed Diagnostics Ltd. | Signatures and determinants for diagnosing infections and methods of use thereof |
| WO2013148868A1 (en) * | 2012-03-30 | 2013-10-03 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the binding, detection, differentiation, isolation and sequencing of herpes simplex virus |
| CN103045761B (zh) * | 2012-12-14 | 2016-01-20 | 山东省眼科研究所 | 用于检测感染性眼病致病微生物的试剂盒及检测方法 |
| WO2014186874A1 (en) | 2013-05-23 | 2014-11-27 | Yyz Pharmatech, Inc. | Methods and compositions for enzyme linked immuno and hybridization mass spectrometric assay |
| CN111624346A (zh) | 2014-08-14 | 2020-09-04 | 米密德诊断学有限公司 | 用于预测及/或决定预后的套组 |
| CN204050005U (zh) * | 2014-09-09 | 2014-12-31 | 赵潺 | 一次性负压前房穿刺针 |
| US20170234873A1 (en) | 2014-10-14 | 2017-08-17 | Memed Diagnostics Ltd. | Signatures and determinants for diagnosing infections in non-human subjects and methods of use thereof |
| US9828627B2 (en) * | 2014-11-05 | 2017-11-28 | Illumina Cambridge Limited | Reducing DNA damage during sample preparation and sequencing using siderophore chelators |
| EP3225696B1 (en) * | 2014-11-19 | 2020-06-03 | Otsuka Pharmaceutical Co., Ltd. | Primer set and method for amplifying exons of pkd1 gene and pkd2 gene |
| CA2968650A1 (en) | 2014-12-11 | 2016-06-16 | Memed Diagnostics Ltd. | Marker combinations for diagnosing infections and methods of use thereof |
| KR101521409B1 (ko) * | 2015-01-15 | 2015-05-18 | 경북대학교 산학협력단 | 진균성 및 아메바성 각막염의 원인체를 동시에 검출할 수 있는 다중 중합효소 연쇄반응 프라이머 세트 및 이를 이용한 각막염 유발균 검출 방법 |
| EP3154439A1 (en) * | 2015-05-19 | 2017-04-19 | Yaya Diagnostics GmbH | Means and methods for the enrichment of nucleic acids |
| US12338497B2 (en) | 2016-03-03 | 2025-06-24 | Memed Diagnostics Ltd. | Analyzing RNA for diagnosing infection type |
| CN108699583B (zh) | 2016-03-03 | 2022-11-01 | 米密德诊断学有限公司 | 用于区分细菌和病毒感染的rna决定子 |
| CN106167833B (zh) * | 2016-05-20 | 2019-09-20 | 南方医科大学 | 一种同时检测8种虫媒脑炎病毒的rt-pcr引物和探针组合及试剂盒 |
| EP3482201B1 (en) | 2016-07-10 | 2022-12-14 | Memed Diagnostics Ltd. | Early diagnosis of infections |
| WO2018011795A1 (en) | 2016-07-10 | 2018-01-18 | Memed Diagnostics Ltd. | Protein signatures for distinguishing between bacterial and viral infections |
| EP3519833B1 (en) | 2016-09-29 | 2024-08-14 | MeMed Diagnostics Ltd. | Methods of prognosis and treatment |
| US11353456B2 (en) | 2016-09-29 | 2022-06-07 | Memed Diagnostics Ltd. | Methods of risk assessment and disease classification for appendicitis |
| CN106834548B (zh) * | 2017-03-30 | 2020-03-27 | 广州基迪奥生物科技有限公司 | 一种常见眼内感染病毒多重实时荧光pcr检测试剂盒 |
| US10209260B2 (en) | 2017-07-05 | 2019-02-19 | Memed Diagnostics Ltd. | Signatures and determinants for diagnosing infections and methods of use thereof |
| US12195810B2 (en) | 2017-12-22 | 2025-01-14 | Massachusetts Eye And Ear Infirmary | Comprehensive microbial panel for molecular diagnosis of eye infections |
| KR102297545B1 (ko) * | 2020-02-06 | 2021-09-02 | 경희대학교 산학협력단 | 코리스메이트 뮤테이즈 단백질 항체를 포함하는 가시아메바성 각막염의 감별 진단용 조성물 및 이를 이용한 가시아메바성 각막염 진단 키트 |
| CN111763767A (zh) * | 2020-06-04 | 2020-10-13 | 上海捷诺生物科技有限公司 | 中枢神经系统感染病原体检测试剂盒及其应用 |
| CN113201599B (zh) * | 2021-06-03 | 2023-03-31 | 北京大学人民医院 | 一种基于PCR和nanopore测序检测脑脊液感染哪些病原体的方法 |
| CN114277167A (zh) * | 2021-12-31 | 2022-04-05 | 杭州千基生物科技有限公司 | 一种用于细菌性肠道病原体的量子点核酸检测的试剂盒 |
| CN114891870A (zh) * | 2022-06-26 | 2022-08-12 | 杭州奥明医学检验实验室有限公司 | 一种基于mNGS检测致癌病原体的方法、系统及装置 |
| CN116144811B (zh) * | 2022-12-21 | 2024-02-20 | 迪飞医学科技(南京)有限公司 | 用于检测脑脊液病原的多重引物组、方法和试剂盒 |
| CN116121428A (zh) * | 2023-03-17 | 2023-05-16 | 郑州安图生物工程股份有限公司 | 引物探针组合及试剂盒 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000073436A1 (en) * | 1999-05-29 | 2000-12-07 | Sj Hightech Co., Ltd. | Oligonucleotide for detection and identification of mycobacteria |
| WO2004099438A1 (en) * | 2003-05-07 | 2004-11-18 | Coris Bioconcept Sprl | One step oligochromatographic device and method of use |
| WO2006080501A1 (ja) * | 2005-01-31 | 2006-08-03 | Kabushiki Kaisha Yakult Honsha | ｒＲＮＡを標的とした微生物の定量的解析方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639871A (en) | 1988-09-09 | 1997-06-17 | Roche Molecular Systems, Inc. | Detection of human papillomavirus by the polymerase chain reaction |
| JPH10323189A (ja) * | 1997-05-23 | 1998-12-08 | Toyobo Co Ltd | 抗酸菌属細菌の検出または菌種同定用オリゴヌクレオチドおよびその用途 |
| US6514694B2 (en) * | 1997-12-19 | 2003-02-04 | Heska Corporation | Methods for the detection of encysted parasites |
| US20040121335A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents associated with host versus graft and graft versus host rejections |
| US20030027135A1 (en) | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
| JP2003144153A (ja) * | 2001-11-09 | 2003-05-20 | Gifu Univ | 遺伝子検出方法、遺伝子検出用プライマー、ｄｎａマイクロアレイ及び遺伝子検出用キット |
| CN1329523C (zh) * | 2002-08-09 | 2007-08-01 | 中国人民解放军基因工程研究所 | 一种限制性荧光标记方法 |
| CN1508264A (zh) * | 2002-12-18 | 2004-06-30 | 云南大学 | 一种临床感染性疾病基因芯片诊断方案 |
| CN1195070C (zh) * | 2003-05-09 | 2005-03-30 | 陶开华 | 一种检测多种传染病的检测型基因芯片及应用 |
| CA2562775C (en) * | 2004-04-07 | 2015-08-04 | Access Bio, Inc. | Nucleic acid detection system |
| JP2006129810A (ja) * | 2004-11-08 | 2006-05-25 | Canon Inc | 感染症起炎菌検出用プローブセット及び担体及び遺伝子検査方法 |
| EP1951888B1 (en) * | 2005-11-09 | 2012-09-05 | PrimeraDx, Inc. | Multiplexed quantitative detection of pathogens |
| CN1896284B (zh) * | 2006-06-30 | 2013-09-11 | 博奥生物有限公司 | 一种鉴别等位基因类型的方法 |
| CN1995387B (zh) * | 2006-11-07 | 2010-06-23 | 亚能生物技术（深圳）有限公司 | 分枝杆菌菌种鉴定基因芯片检测系统 |
| CA2689306C (en) * | 2007-06-01 | 2019-01-15 | Council Of Scientific & Industrial Research | A novel method for simultaneous detection and discrimination of bacterial, fungal, parasitic and viral infections of eye and central nervous system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000073436A1 (en) * | 1999-05-29 | 2000-12-07 | Sj Hightech Co., Ltd. | Oligonucleotide for detection and identification of mycobacteria |
| WO2004099438A1 (en) * | 2003-05-07 | 2004-11-18 | Coris Bioconcept Sprl | One step oligochromatographic device and method of use |
| WO2006080501A1 (ja) * | 2005-01-31 | 2006-08-03 | Kabushiki Kaisha Yakult Honsha | ｒＲＮＡを標的とした微生物の定量的解析方法 |
Non-Patent Citations (2)
| Title |
|---|
| L. L. Dworkin et al., "Real-time Quantitative Polymerase Chain Reaction Diagnosis of Infectious Posterior Uveitis", Archives of Ophthalmology, Nov. 2002, Vol. 120, page 1534~1539. * |
| T. Suzuki et al., "Application of Multiplex-PCR and DNA-microarray techniques for identifying conjunctivitis-causing pathogens", Invest. Ophthalmol. Vis. Sci., Apr. 2004, Vol. 45, E-Abstract, page 1656. * |
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