AR066825A1 - Metodo para la deteccion y la discriminacion simultanea de infecciones bacterianas, fungicas, parasitarias y virales en los ojos y el sistema nervioso central por pcr - Google Patents

Metodo para la deteccion y la discriminacion simultanea de infecciones bacterianas, fungicas, parasitarias y virales en los ojos y el sistema nervioso central por pcr

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Publication number
AR066825A1
AR066825A1 ARP080102330A ARP080102330A AR066825A1 AR 066825 A1 AR066825 A1 AR 066825A1 AR P080102330 A ARP080102330 A AR P080102330A AR P080102330 A ARP080102330 A AR P080102330A AR 066825 A1 AR066825 A1 AR 066825A1
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seq
primer
direct
reverse primer
direct primer
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ARP080102330A
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English (en)
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Sharma Savitri
Satpathy Gita
Naraharira O Madhavan Hajib
Sridhar Rao Kunchala
Mohan Rao Chintalagiri
Ramchander Puppala
Kumar Venka Ravi
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Council Scient Ind Res
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract

Métodos de diagnostico para la identificacion de uno o más agentes causales de infecciones oculares y del sistema nervioso entre una multiplicidad de patogenos probables que podrían provocar la infeccion. Todos los patogenos que afectan un área discreta de los ojos o el sistema nervioso generalmente provocan las mismas manifestaciones clínicas o síndromes. Se relaciona con la deteccion y la discriminacion del patogeno entre un conjunto de patogenos probables en una sola prueba, sin recurrir a una batería de pruebas, cada una dirigida a la deteccion de un solo patogeno. Se centra en el diagnostico basado en el síndrome, que reemplaza al diagnostico basado en la deteccion de patogenos individuales. Reivindicacion 1: Conjuntos de cebadores utiles para la deteccion y la discriminacion de patogenos que causan síndromes, caracterizados porque: el conjunto 1 es cebador directo: 5' cgcttggtttcggatgggag 3' (SEQ ID N° 1) cebador inverso: 5' gcccccagagacttgttgtagg 3' (SEQ ID N° 2); el conjunto 2 es cebador directo: 5' ggcaatcgtgtacgtcgtccg 3' (SEQ ID N° 3) cebador inverso: 5' cgggggggtcttgcgttac 3' (SEQ ID N° 4); el conjunto 3 es cebador directo: 5' caagctgacggacatttacaagg 3' (SEQ ID N° 5) cebador inverso: 5' gtcccacacgcgaaacacg 3' (SEQ ID N° 6); el conjunto 4 es cebador directo: 5' ttccggctcatggcgttaacc 3' (SEQ ID N° 7) cebador inverso: 5' cgccctgcttttacgttacgc 3' (SEQ ID N° 8); el conjunto 5 es cebador directo: 5' cggcgacgacgacgataaag 3' (SEQ ID N° 9) cebador inverso: 5' caatctggtcgcgtaatcctctg 3' (SEQ ID N° 10); el conjunto 6 es cebador directo: 5' gggcacgtcctcgcagaag 3' (SEQ ID N° 11) cebador inverso: 5' ccaagatgcaggtgataggtgac 3' (SEQ ID N° 12); el conjunto 7 es cebador directo: 5' ggtcttgccggagctggtattac 3' (SEQ ID N° 13) cebador inverso: 5' tgcctccgtgaaagacaaagaca 3' (SEQ ID N° 14); el conjunto 8 es cebador directo: 5' tccatttaacgttgcatcattttgtg 3' (SEQ ID N° 15) cebador inverso: 5' acgttccggtagcgagttatctg 3' (SEQ ID N° 16); el conjunto 9 es cebador directo: 5' cgccgccaacatgctctacc 3' (SEQ ID N° 17) cebador inverso: 5' gttgcgggaggggatggata 3' (SEQ ID N° 18); el conjunto 10 es cebador directo: 5' tgggctacacacgtgctacaatgg 3' (SEQ ID N° 19) cebador inverso: 5' cggactacgatcggttttgtgaga 3' (SEQ ID N° 20); el conjunto 11 es cebador directo: 5' ggcctaacacatgcaagtcgagc 3' (SEQ ID N° 21) cebador inverso: 5' ggcagattcctaggcattactcacc 3' (SEQ ID N° 22); el conjunto 12 es cebador directo: 5' acgtcaaatcatcatgcccccttat 3' (SEQ ID N° 23) cebador inverso: 5' tgcagccctttgtaccgtccat 3' (SEQ ID N° 24); el conjunto 13 es cebador directo: 5' gcggaacgtgggaccaatac 3' (SEQ ID N° 25) cebador inverso: 5' cgacggggtgattttcttcttc 3' (SEQ ID N° 26); el conjunto 14 es cebador directo: 5' aacttttttgactgccagacacactattg 3' (SEQ ID N° 27) cebador inverso: 5' ggatgccaccccccaaaag 3' (SEQ ID N° 28); el conjunto 15 es cebador directo: 5' tggttactcgcttggtgaatatgt 3' (SEQ ID N° 29) cebador inverso: 5' gacgttttgccgactacctatcc 3' (SEQ ID N° 30); el conjunto 16 es cebador directo: 5' cccctctgctggcgaaaagtg 3' (SEQ ID N° 31) cebador inverso: 5' ggcgaccaatctgcgaatacac 3' (SEQ ID N° 32); el conjunto 17 es cebador directo: 5' aatcgtatctcgggttaatgttgc 3' (SEQ ID N° 33) cebador inverso: 5' tcgaggaaaaccgtatgagaaac 3' (SEQ ID N° 34); el conjunto 18 es cebador directo: 5' gctgggactgaggactgcgac 3' (SEQ ID N° 35) cebador inverso: 5' ttcaagacgggcggcatataac 3' (SEQ ID N° 36); el conjunto 19 es cebador directo: 5' tggcgaacgggtgagtaaca 3' (SEQ ID N° 37) cebador inverso: 5' ccggtattagccccagtttcc 3' (SEQ ID N° 38); el conjunto 20 es cebador directo: 5' cggcggcaagttcgacgac 3' (SEQ ID N° 39) cebador inverso: 5' ccaccgagacgcccacacc 3' (SEQ ID N° 40); el conjunto 21 es cebador directo: 5' ccaggtcggcggagaagc 3' (SEQ ID N° 41) cebador inverso: 5' ccaccggcccgatgacc 3' (SEQ ID N° 42); el conjunto 22 es cebador directo: 5' gccgccctgaccaccttc 3 (SEQ ID N° 43) cebador inverso: 5' gcgggttgttcggcatcag 3' (SEQ ID N° 44), donde dichos cebadores se usan solos o en combinacion. Reivindicacion 6: Un método para la deteccion y la discriminacion de patogenos que causan síndromes tales como endoftalmitis infecciosa, queratitis, uveítis, retinitis o meningitis, caracterizado porque comprende amplificar genes específicos de dichos patogenos con un ensayo de PCR (reaccion en cadena de la polimerasa) multiple en la muestra clínica, usando el conjunto de cebadores directos e inversos unicos reivindicados en la reivindicacion 1, y en pasos posteriores, hacer hibridizar el o los productos amplificados (sondas) con secuencias de ADN complementarias (blancos) inmovilizadas en una matriz de fase solida en un formato multiple, de acuerdo con métodos conocidos, y detectar el o los productos hibridizados de acuerdo con métodos conocidos, con el fin de detectar y discriminar el patogeno específico que causa el síndrome investigado.
ARP080102330A 2007-06-01 2008-06-02 Metodo para la deteccion y la discriminacion simultanea de infecciones bacterianas, fungicas, parasitarias y virales en los ojos y el sistema nervioso central por pcr AR066825A1 (es)

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