SK287706B6 - Production of recombinant blood clotting factors in human cell lines - Google Patents

Production of recombinant blood clotting factors in human cell lines Download PDF

Info

Publication number
SK287706B6
SK287706B6 SK1504-2002A SK15042002A SK287706B6 SK 287706 B6 SK287706 B6 SK 287706B6 SK 15042002 A SK15042002 A SK 15042002A SK 287706 B6 SK287706 B6 SK 287706B6
Authority
SK
Slovakia
Prior art keywords
factor viii
ser
leu
glu
vector
Prior art date
Application number
SK1504-2002A
Other languages
English (en)
Slovak (sk)
Other versions
SK15042002A3 (sk
Inventor
Charlotte Hauser
Andrea Horster
Carola Schroder
Michael Lehnerer
Original Assignee
Octapharma Biopharmaceuticals Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00106225A external-priority patent/EP1136553A1/en
Application filed by Octapharma Biopharmaceuticals Gmbh filed Critical Octapharma Biopharmaceuticals Gmbh
Publication of SK15042002A3 publication Critical patent/SK15042002A3/sk
Publication of SK287706B6 publication Critical patent/SK287706B6/sk

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SK1504-2002A 2000-03-22 2001-03-21 Production of recombinant blood clotting factors in human cell lines SK287706B6 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00106225A EP1136553A1 (en) 2000-03-22 2000-03-22 Production of recombinant blood clotting factors in human cell lines
US20324900P 2000-05-08 2000-05-08
PCT/EP2001/003220 WO2001070968A2 (en) 2000-03-22 2001-03-21 Production of recombinant blood clotting factors in human cell lines

Publications (2)

Publication Number Publication Date
SK15042002A3 SK15042002A3 (sk) 2003-09-11
SK287706B6 true SK287706B6 (en) 2011-07-06

Family

ID=26070708

Family Applications (1)

Application Number Title Priority Date Filing Date
SK1504-2002A SK287706B6 (en) 2000-03-22 2001-03-21 Production of recombinant blood clotting factors in human cell lines

Country Status (28)

Country Link
US (1) US7572619B2 (pt)
EP (2) EP1460131A3 (pt)
JP (1) JP3894795B2 (pt)
KR (1) KR100581574B1 (pt)
CN (1) CN1454257B (pt)
AT (1) ATE312176T1 (pt)
AU (3) AU2001254715B2 (pt)
BE (1) BE2014C077I2 (pt)
BG (1) BG65930B1 (pt)
BR (1) BRPI0109494B8 (pt)
CA (1) CA2404163C (pt)
CZ (1) CZ303929B6 (pt)
DE (1) DE60115613T2 (pt)
DK (1) DK1266006T3 (pt)
EA (1) EA004317B1 (pt)
EE (1) EE200200538A (pt)
ES (1) ES2254403T3 (pt)
FR (1) FR15C0003I2 (pt)
HR (1) HRP20020767B1 (pt)
HU (1) HU228091B1 (pt)
IL (2) IL151857A0 (pt)
MX (1) MXPA02009221A (pt)
NO (1) NO330910B1 (pt)
NZ (1) NZ521732A (pt)
RS (1) RS50743B (pt)
SI (1) SI1266006T1 (pt)
SK (1) SK287706B6 (pt)
WO (1) WO2001070968A2 (pt)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA02009221A (es) * 2000-03-22 2005-07-25 Octagene Gmbh Produccion de factores de coagulacion sanguineo recombinantes en lineas celular humanas.
EP1319016A4 (en) * 2000-09-19 2006-05-10 Univ Emory MODIFIED FACTOR VIII
EP1469064A1 (en) 2003-04-15 2004-10-20 DRK-Blutspendedienst Baden-Württemberg-Hessen gGmbH Expression of proteins in endothelial cells derived from precursor cells from cord blood
EP1755652B1 (en) * 2004-03-19 2010-11-24 Baxter International Inc. Factor ixa for the treatment of bleeding disorders
KR100624013B1 (ko) * 2004-06-25 2006-09-19 주식회사 녹십자홀딩스 동결건조된 알부민 비함유 재조합 사람 혈액응고 제 8인자 제제
DK1804839T3 (da) 2004-09-22 2012-04-10 St Jude Childrens Res Hospital Forbedret ekspression af faktor ix i genterapivektorer
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
WO2006084826A1 (en) * 2005-02-11 2006-08-17 Novo Nordisk Health Care Ag Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
EP1739179A1 (en) 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
PT2495307T (pt) 2006-07-13 2018-05-10 Wyeth Llc Produção do fator ix de coagulação com padrão melhorado de glicosilação
AU2008245524A1 (en) * 2007-04-26 2008-11-06 Cnj Holdings, Inc. Recombinant vitamin K dependent proteins with high sialic acid content and methods of preparing same
US20110045535A1 (en) * 2007-08-27 2011-02-24 National University Corporation Nagoya University Activator for Blood Coagulation Factor VII Promoter and Utilization of the Same
RU2011110459A (ru) 2008-08-21 2012-09-27 Октафарма АГ (CH) Рекомбинантно полученный человеческий фактор viii и ix
EP3243835B1 (en) 2009-02-11 2024-04-10 Albumedix Ltd Albumin variants and conjugates
WO2011041770A1 (en) 2009-10-02 2011-04-07 The Children's Hospital Of Philadelphia Compositions and methods for enhancing coagulation factor viii function
CA2776241A1 (en) 2009-10-30 2011-05-05 Novozymes Biopharma Dk A/S Albumin variants
WO2011124718A1 (en) 2010-04-09 2011-10-13 Novozymes A/S Albumin derivatives and variants
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
MX352889B (es) 2011-02-25 2017-12-13 Chugai Pharmaceutical Co Ltd Anticuerpo de fc especifico para fcyriib.
CN102199607B (zh) * 2011-03-30 2012-11-14 山西大学 重组人凝血因子ix小基因及其ptc突变体稳定细胞株
CA2835053C (en) 2011-05-13 2020-07-07 Octapharma Ag A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii
TW201307563A (zh) * 2011-05-19 2013-02-16 Shire Human Genetic Therapies 純化乙醯肝素－ｎ－硫酸酯酶之方法
CN102321668A (zh) * 2011-07-06 2012-01-18 中国人民解放军军事医学科学院野战输血研究所 一种表达重组人凝血因子ⅶ的方法及其专用载体
US20150050269A1 (en) 2011-09-30 2015-02-19 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
WO2013057219A1 (en) 2011-10-18 2013-04-25 Csl Behring Gmbh Method for improving the stability of purified factor viii after reconstitution
EP2780364A2 (en) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteins with improved half-life and other properties
MX358220B (es) 2011-11-30 2018-08-10 Chugai Pharmaceutical Co Ltd Portador que contiene fármaco en la célula para formar el inmunocomplejo.
US9944691B2 (en) 2012-03-16 2018-04-17 Albumedix A/S Albumin variants
EP2830646B1 (en) 2012-03-27 2018-03-07 NGM Biopharmaceuticals, Inc. Compositions and methods of use for treating metabolic disorders
GB201210357D0 (en) 2012-06-12 2012-07-25 Ucl Business Plc Factor VIII sequences
US8986991B2 (en) * 2012-07-03 2015-03-24 Expression Therapeutics, Llc High yield suspension cell line, system and method for making same
EP3404105A1 (en) 2012-07-06 2018-11-21 Bioverativ Therapeutics Inc. Cell line expressing single chain factor viii polypeptides and uses thereof
US20140128326A1 (en) 2012-11-08 2014-05-08 Novozymes Biopharma Dk A/S Albumin variants
CA2898975C (en) * 2013-01-24 2021-06-15 Portola Pharmaceuticals, Inc. Inhibition of tissue factor pathway inhibitor with factor xa derivatives
AU2014250434B2 (en) 2013-04-02 2019-08-08 Chugai Seiyaku Kabushiki Kaisha Fc region variant
EP2853538A1 (en) 2013-09-27 2015-04-01 Université Pierre et Marie Curie (Paris 6) Analogues of temporin-SHa and uses thereof
SG11201700378PA (en) 2014-07-30 2017-02-27 Ngm Biopharmaceuticals Inc Compositions and methods of use for treating metabolic disorders
EP3701969A1 (en) 2014-10-31 2020-09-02 NGM Biopharmaceuticals, Inc. Compositions and methods of use for treating metabolic disorders
GB201420139D0 (en) 2014-11-12 2014-12-24 Ucl Business Plc Factor IX gene therapy
SG11201700841QA (en) 2014-12-19 2017-03-30 Chugai Pharmaceutical Co Ltd Anti-myostatin antibodies, polypeptides containing variant fc regions, and methods of use
BR102015012334A2 (pt) 2015-05-27 2016-11-29 Fundação Hemoct De Ribeirão Preto Fundherp processo de produção do fator vii de coagulação sanguínea e fator vii de coagulação sanguínea
AR104956A1 (es) 2015-06-09 2017-08-30 Glycotope Gmbh MÉTODO MEJORADO PARA LA PRODUCCIÓN DE POLIPÉPTIDOS g-CARBOXILADOS
BR112018003179A2 (pt) 2015-08-20 2018-09-25 Albumedix As conjugados e variantes de albumina
CN105219739A (zh) * 2015-09-21 2016-01-06 北京神源德生物科技有限公司 重组单纯疱疹病毒及它感染和制备它的宿主细胞以及它们的应用
CN109562191A (zh) 2016-04-15 2019-04-02 宾夕法尼亚州大学信托人 用于治疗血友病a的基因疗法
MX2019001448A (es) 2016-08-05 2019-09-13 Chugai Pharmaceutical Co Ltd Composicion para profilaxis o tratamiento de enfermedades relacionadas con interleucina 8 (il-8).
EP3625329A1 (en) 2017-05-17 2020-03-25 Octapharma AG Method for the production of a recombinant target protein
CN111556895B (zh) 2017-11-14 2024-09-13 中外制药株式会社 抗-c1s抗体及使用方法
WO2020020364A1 (zh) * 2018-07-26 2020-01-30 正大天晴药业集团南京顺欣制药有限公司 一种制备重组人凝血因子ⅷ的方法
US10842885B2 (en) 2018-08-20 2020-11-24 Ucl Business Ltd Factor IX encoding nucleotides
WO2022259007A1 (en) 2021-06-11 2022-12-15 Sorbonne Universite Short antimicrobial peptides

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI86885C (fi) * 1984-04-20 1992-10-26 Genentech Inc Foerfarande foer framstaellning av human rekombinantfaktor viii och nukleinsyrasekvenser och vektorer anvaend daertill
JPS6171774A (ja) * 1984-09-14 1986-04-12 Sony Corp テレビジヨンカメラ装置のビ−ム電流制御装置
AU5864086A (en) 1985-04-22 1986-11-18 Genetics Institute Inc. High yield production of active factor ix
JP2525022B2 (ja) 1986-01-03 1996-08-14 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド ▲ＶＩＩＩ▼：ｃ因子型タンパク質の改良生産方法
US5451521A (en) * 1986-05-29 1995-09-19 Genetics Institute, Inc. Procoagulant proteins
US5422260A (en) * 1986-05-29 1995-06-06 Genetics Institute, Inc. -Legal Affairs Human factor VIII:c muteins
US5024939A (en) 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
FR2638643B1 (fr) * 1988-11-09 1991-04-12 Transgene Sa Sequence d'adn codant pour le facteur ix humain ou une proteine analogue, vecteur d'expression, cellules transformees, procede de preparation du facteur ix et produits obtenus correspondants
ES2112255T3 (es) * 1989-11-17 1998-04-01 Novo Nordisk As Complejos proteicos que tienen actividad del factor viii:c y su produccion.
SE465222C5 (sv) * 1989-12-15 1998-02-10 Pharmacia & Upjohn Ab Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
EP0512011B1 (en) * 1990-01-26 1994-04-06 IMMUNO Aktiengesellschaft Recombinantly produced blood factors and process for the expression of said blood factors, as well as vaccinia virus recombinants used in said process
US5445953A (en) * 1991-08-26 1995-08-29 Immuno Aktiengesellschaft Direct molecular cloning of a modified poxvirus genome
ATE322547T1 (de) * 1993-06-10 2006-04-15 Genetic Therapy Inc Adenovirale vektoren für die behandlung der hämophilie
EP0915975A2 (en) * 1996-07-03 1999-05-19 Chiron Corporation Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia
US6114148C1 (en) 1996-09-20 2012-05-01 Gen Hospital Corp High level expression of proteins
JP2002517180A (ja) * 1997-12-05 2002-06-18 ジ・イミユーン・リスポンス・コーポレーシヨン 増大された発現を示す新規ベクターおよび遺伝子
US6358703B1 (en) * 1998-12-10 2002-03-19 Bayer Corporation Expression system for factor VIII
EP1151099A1 (en) * 1999-02-19 2001-11-07 Octagene GmbH Hormone-hormone receptor complexes and nucleic acid constructs and their use in gene therapy
WO2001012836A1 (en) * 1999-08-13 2001-02-22 Fred Hutchinson Cancer Research Center Crystal of a truncated protein construct containing a coagulation factor viii c2 domain in the presence or absence of a bound ligand and methods of use thereof
MXPA02009221A (es) * 2000-03-22 2005-07-25 Octagene Gmbh Produccion de factores de coagulacion sanguineo recombinantes en lineas celular humanas.
WO2003009237A1 (en) * 2001-07-18 2003-01-30 Skaginn Hf. A method and apparatus for relating information of a processed object to an operator

Also Published As

Publication number Publication date
US7572619B2 (en) 2009-08-11
FR15C0003I2 (fr) 2015-11-20
IL151857A0 (en) 2003-04-10
MXPA02009221A (es) 2005-07-25
AU2006201848A1 (en) 2006-05-25
IL151857A (en) 2010-05-31
HU228091B1 (en) 2012-10-29
US20040023333A1 (en) 2004-02-05
NO20024475D0 (no) 2002-09-19
CZ20023166A3 (cs) 2003-02-12
EP1460131A3 (en) 2005-06-01
ES2254403T3 (es) 2006-06-16
WO2001070968A3 (en) 2001-12-13
EE200200538A (et) 2004-04-15
DE60115613D1 (de) 2006-01-12
EA004317B1 (ru) 2004-02-26
SK15042002A3 (sk) 2003-09-11
DE60115613T2 (de) 2006-08-24
KR20030011079A (ko) 2003-02-06
RS50743B (sr) 2010-08-31
BE2014C077I2 (pt) 2020-01-30
BG65930B1 (bg) 2010-05-31
ATE312176T1 (de) 2005-12-15
BRPI0109494B1 (pt) 2021-05-11
KR100581574B1 (ko) 2006-05-22
JP2003530093A (ja) 2003-10-14
NO330910B1 (no) 2011-08-15
HRP20020767A2 (en) 2005-02-28
JP3894795B2 (ja) 2007-03-22
CN1454257A (zh) 2003-11-05
CA2404163C (en) 2009-09-29
YU71002A (sh) 2005-11-28
NZ521732A (en) 2004-05-28
HUP0300588A3 (en) 2005-09-28
EP1266006A2 (en) 2002-12-18
AU2001254715B2 (en) 2006-02-02
HUP0300588A2 (hu) 2003-06-28
DK1266006T3 (da) 2006-01-16
HRP20020767B1 (en) 2011-09-30
CN1454257B (zh) 2013-01-16
FR15C0003I1 (fr) 2015-02-27
CZ303929B6 (cs) 2013-07-03
EA200201008A1 (ru) 2003-02-27
CA2404163A1 (en) 2001-09-27
WO2001070968A2 (en) 2001-09-27
EP1266006B1 (en) 2005-12-07
SI1266006T1 (sl) 2006-06-30
EP1460131A2 (en) 2004-09-22
BG107152A (bg) 2003-06-30
AU5471501A (en) 2001-10-03
BR0109494A (pt) 2002-12-10
BRPI0109494B8 (pt) 2021-05-25
NO20024475L (no) 2002-11-20

Similar Documents

Publication Publication Date Title
CA2404163C (en) Production of recombinant blood clotting factors in human cell lines
JP6909203B2 (ja) 第ｉｘ因子融合タンパク質及びそれらの製造方法及び使用方法
JP2888518B2 (ja) 改良型の組換え発現
AU2016337408A1 (en) Inducible modification of a cell genome
AU2021204620A1 (en) Central nervous system targeting polynucleotides
CN105960413B (zh) 人工dna-结合蛋白及其用途
JP2749083B2 (ja) 活性化されたヒトプロテインｃの直接発現のためのベクターおよび化合物
PT1863920E (pt) Método para isolamento melhorado de proteínas recombinantemente produzidas
AU2001254715A1 (en) Production of recombinant blood clotting factors in human cell lines
AU2016343979A1 (en) Delivery of central nervous system targeting polynucleotides
CN107460201A (zh) 编码gpc‑3嵌合抗原受体蛋白的核酸及表达gpc‑3嵌合抗原受体蛋白的t淋巴细胞
JPH0640942A (ja) 機能的な第ｖｉｉｉ因子の製造方法
NO173741B (no) Dna-sekvens for modifisert protein c, vektor inneholdendedenne, pattedyrceller transfektert med vektorer, og fremgangsmaate for fremstilling av nevnte protein
CN111733174B (zh) 一种分离的核酸分子及其用途
CA2407881A1 (en) Vectors for ocular transduction and use thereof for genetic therapy
KR101921698B1 (ko) 재조합에 의해 제조된 인간 인자 ⅷ 및 ⅸ
CN107849583B (zh) 使用细胞分裂基因座控制细胞增殖的工具和方法
CN112912112A (zh) 肝特异性核酸调节元件以及其方法及用途
CN113817775A (zh) 修饰的阿柏西普、组合物、方法及其在基因治疗中的应用
TW202317753A (zh) 經武裝之嵌合受體及其使用方法
PL207713B1 (pl) Muteina czynnika VIII, kodująca ją sekwencja DNA, wektor obejmujący sekwencję DNA, komórka gospodarza stransformowana tym wektorem, sposób wytwarzania muteiny czynnika VIII, kompozycja farmaceutyczna, zastosowanie muteiny czynnika VIII lub wektora i linia unieśmiertelnionych komórek ludzkich
JP3422963B2 (ja) ペプチドおよびその用途
TWI541252B (zh) 因子ｖｉｉ／ｖｉｉａ之量產方法
CN113260704B (zh) CYP4V2和RdCVF在制备药物中的用途
KR20230117177A (ko) 진행성 골화성 섬유이형성증을 위한 신규 유전자 치료제의개발

Legal Events

Date Code Title Description
TC4A Change of owner's name

Owner name: OCTAPHARMA BIOPHARMACEUTICALS GMBH, MUNICH, DE

Effective date: 20101108

TE4A Change of owner's address

Owner name: OCTAPHARMA BIOPHARMACEUTICALS GMBH, MARTINSRIE, DE

Effective date: 20101108

PC4A Assignment and transfer of rights

Owner name: OCTAPHARMA AG, LACHEN, CH

Free format text: FORMER OWNER: OCTAPHARMA BIOPHARMACEUTICALS GMBH, MARTINSRIED, DE

Effective date: 20140819

MK4A Patent expired

Expiry date: 20210321