RU2541429C2 - Хроматографический лиганд - Google Patents

Хроматографический лиганд Download PDF

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Publication number
RU2541429C2
RU2541429C2 RU2010110437/04A RU2010110437A RU2541429C2 RU 2541429 C2 RU2541429 C2 RU 2541429C2 RU 2010110437/04 A RU2010110437/04 A RU 2010110437/04A RU 2010110437 A RU2010110437 A RU 2010110437A RU 2541429 C2 RU2541429 C2 RU 2541429C2
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RU
Russia
Prior art keywords
matrix
antibodies
ligand
separation matrix
column
Prior art date
Application number
RU2010110437/04A
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English (en)
Russian (ru)
Other versions
RU2010110437A (ru
Inventor
Карина ЭНГСТРАНД
Анника ФОРСС
Гуннар ГЛАД
Бо-Леннарт ЙОХАНССОН
Ханс Й. ЙОХАНССОН
Жан-Люк МАЛУАЗЕЛЬ
Original Assignee
Джи-И Хелткер Байо-Сайенсиз АБ
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Priority claimed from SE0402558A external-priority patent/SE0402558D0/xx
Application filed by Джи-И Хелткер Байо-Сайенсиз АБ filed Critical Джи-И Хелткер Байо-Сайенсиз АБ
Publication of RU2010110437A publication Critical patent/RU2010110437A/ru
Application granted granted Critical
Publication of RU2541429C2 publication Critical patent/RU2541429C2/ru

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04—Processes using organic exchangers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2010110437/04A 2004-10-21 2005-10-21 Хроматографический лиганд RU2541429C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0402558A SE0402558D0 (sv) 2004-10-21 2004-10-21 A process for the purification of antibodies
SE0402558-1 2004-10-21
SE0402910 2004-11-26
SE0402910-4 2004-11-26

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU2007112990/04A Division RU2396246C2 (ru) 2004-10-21 2005-10-21 Хроматографический лиганд

Publications (2)

Publication Number Publication Date
RU2010110437A RU2010110437A (ru) 2011-09-27
RU2541429C2 true RU2541429C2 (ru) 2015-02-10

Family

ID=36203226

Family Applications (3)

Application Number Title Priority Date Filing Date
RU2010110437/04A RU2541429C2 (ru) 2004-10-21 2005-10-21 Хроматографический лиганд
RU2007112991/15A RU2389552C2 (ru) 2004-10-21 2005-10-21 Способ очистки антител
RU2007112990/04A RU2396246C2 (ru) 2004-10-21 2005-10-21 Хроматографический лиганд

Family Applications After (2)

Application Number Title Priority Date Filing Date
RU2007112991/15A RU2389552C2 (ru) 2004-10-21 2005-10-21 Способ очистки антител
RU2007112990/04A RU2396246C2 (ru) 2004-10-21 2005-10-21 Хроматографический лиганд

Country Status (12)

Country Link
US (5) US7867784B2 (enExample)
EP (2) EP1807205B1 (enExample)
JP (2) JP5064225B2 (enExample)
KR (2) KR101243425B1 (enExample)
AU (2) AU2005296352B2 (enExample)
BR (2) BRPI0518216C8 (enExample)
CA (2) CA2582113C (enExample)
DK (1) DK1827691T3 (enExample)
ES (1) ES2612572T3 (enExample)
PL (1) PL1827691T3 (enExample)
RU (3) RU2541429C2 (enExample)
WO (2) WO2006043895A1 (enExample)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1715948B1 (en) 2004-01-20 2016-11-23 Pall Corporation Chromatographic material for the absorption of proteins at physiological ionic strength
WO2006043895A1 (en) 2004-10-21 2006-04-27 Ge Healthcare Bio-Sciences Ab A method of antibody purification
US9082456B2 (en) 2005-01-31 2015-07-14 The Invention Science Fund I Llc Shared image device designation
US20060170956A1 (en) 2005-01-31 2006-08-03 Jung Edward K Shared image devices
US9124729B2 (en) 2005-01-31 2015-09-01 The Invention Science Fund I, Llc Shared image device synchronization or designation
US9325781B2 (en) 2005-01-31 2016-04-26 Invention Science Fund I, Llc Audio sharing
US8606383B2 (en) 2005-01-31 2013-12-10 The Invention Science Fund I, Llc Audio sharing
US20060174203A1 (en) 2005-01-31 2006-08-03 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Viewfinder for shared image device
US8902320B2 (en) 2005-01-31 2014-12-02 The Invention Science Fund I, Llc Shared image device synchronization or designation
US9910341B2 (en) 2005-01-31 2018-03-06 The Invention Science Fund I, Llc Shared image device designation
US9489717B2 (en) 2005-01-31 2016-11-08 Invention Science Fund I, Llc Shared image device
TW200639190A (en) * 2005-02-04 2006-11-16 Showa Denko Kk Packing material for ion chromatography
US9967424B2 (en) 2005-06-02 2018-05-08 Invention Science Fund I, Llc Data storage usage protocol
US9819490B2 (en) 2005-05-04 2017-11-14 Invention Science Fund I, Llc Regional proximity for shared image device(s)
US8681225B2 (en) 2005-06-02 2014-03-25 Royce A. Levien Storage access technique for captured data
US9093121B2 (en) 2006-02-28 2015-07-28 The Invention Science Fund I, Llc Data management of an audio data stream
US9001215B2 (en) 2005-06-02 2015-04-07 The Invention Science Fund I, Llc Estimating shared image device operational capabilities or resources
US9942511B2 (en) 2005-10-31 2018-04-10 Invention Science Fund I, Llc Preservation/degradation of video/audio aspects of a data stream
US10003762B2 (en) 2005-04-26 2018-06-19 Invention Science Fund I, Llc Shared image devices
US9451200B2 (en) 2005-06-02 2016-09-20 Invention Science Fund I, Llc Storage access technique for captured data
EP2738178A1 (en) 2006-04-05 2014-06-04 AbbVie Biotechnology Ltd Antibody purification
EP2043767B1 (en) 2006-07-14 2020-03-25 Wisconsin Alumni Research Foundation Adsorptive membranes for trapping viruses
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
JP2010515917A (ja) * 2007-01-10 2010-05-13 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー クロマトグラフィーレジン
CN101765458B (zh) 2007-07-25 2014-05-28 通用电气健康护理生物科学股份公司 分离基质
US8653246B2 (en) * 2007-10-26 2014-02-18 Asahi Kasei Chemicals Corporation Method for purifying protein
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
US8188242B2 (en) * 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
DE102008018734B4 (de) * 2008-04-14 2013-03-21 Sartorius Stedim Biotech Gmbh Hydrophobe Cellulose-Membran, Verfahren zu ihrer Herstellung und ihre Verwendung in der hydrophoben Interaktionschromatographie
DE102008018732B4 (de) * 2008-04-14 2022-06-09 Sartorius Stedim Biotech Gmbh Verfahren zur Stofftrennung unter Verwendung einer Cellulosehydrat-Membran in der Größenausschlusschromatographie
DE102008055821A1 (de) * 2008-04-14 2009-10-15 Sartorius Stedim Biotech Gmbh Cellulosehydrat-Membran, Verfahren zur ihrer Herstellung und Verwendung davon
FR2931838B1 (fr) 2008-06-02 2010-06-11 Millipore Corp Installation pour traiter un liquide biologique.
EP2326658A4 (en) * 2008-09-12 2013-04-10 Ge Healthcare Bio Sciences Ab REMOVAL OF REINFORCED PROTEIN AGGREGATES WITH MULTIMODAL ANION DERIVATIVES IN THE PRESENCE OF TWITTERS WITHOUT PROTEINS
EP2360183B1 (en) * 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antibody purification method by mixed mode chromatography utilizing an arginine-containing loading solution
FR2940145B1 (fr) 2008-12-24 2011-03-25 Millipore Corp Chariot et installation de traitement d'un liquide biologique
JP2010158624A (ja) * 2009-01-08 2010-07-22 Asahi Kasei Chemicals Corp 多孔質吸着膜、及び当該多孔質吸着膜を用いたたんぱく質の精製方法
FR2941385B1 (fr) 2009-01-23 2011-04-01 Millipore Corp Procede pour fournir un circuit pour liquide biologique et circuit obtenu.
US9441011B2 (en) 2009-07-03 2016-09-13 Asahi Kasei Chemicals Corporation Method for purification of antibody using porous membrane having amino group and alkyl group both bound to graft chain immobilized on porous substrate
EP2459308A1 (en) 2009-07-28 2012-06-06 instrAction GmbH Specific sorbent for binding proteins and peptides, and separation method using the same
US8277649B2 (en) * 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
FR2955119B1 (fr) 2010-01-13 2012-12-28 Millipore Corp Circuit pour liquide biologique
MY161534A (en) 2010-05-25 2017-04-28 Genentech Inc Methods of purifying polypeptides
FR2960795B1 (fr) 2010-06-08 2012-07-27 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2960796B1 (fr) 2010-06-08 2014-01-24 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2960794B1 (fr) 2010-06-08 2012-07-27 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2961711B1 (fr) 2010-06-23 2012-08-17 Millipore Corp Poche pour circuit d'une installation de traitement de liquide biologique
FR2961713B1 (fr) 2010-06-23 2012-08-10 Millipore Corp Poche pour circuit d'une installation de traitement de liquide biologique
EP2598223B1 (en) 2010-07-30 2022-04-06 EMD Millipore Corporation Chromatography media and method
FR2963573B1 (fr) 2010-08-03 2012-08-31 Millipore Corp Chariot de pompage pour une installation de traitement de liquide biologique
BR112013006403A2 (pt) * 2010-09-20 2015-09-29 Abbvie Inc purificação de anticorpos usando cromatografia de leito móvel simulado
MX357821B (es) 2010-12-21 2018-07-25 Hoffmann La Roche Preparación de anticuerpo enriquecida con isoforma y método para obtenerla.
FR2973396B1 (fr) 2011-03-28 2013-05-10 Millipore Corp Installation de traitement de liquide biologique
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2570185A1 (en) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbent comprising an aromatic ring system on its surface for the purification of organic molecules
EP2570184A1 (en) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbent comprising on its surface an aromatic ring system having an anionic or deprotonizable group for the purification of organic molecules
US8802448B2 (en) 2011-07-27 2014-08-12 Pall Corporation Mixed mode ligands
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
AU2012351751B2 (en) * 2011-10-19 2017-09-07 Novimmune S.A. Methods of purifying antibodies
US9309282B2 (en) 2011-10-19 2016-04-12 Bio-Rad Laboratories, Inc. Solid phase for mixed-mode chromatographic purification of proteins
EP2773439A4 (en) * 2011-10-31 2015-07-01 Merck Sharp & Dohme CHROMATOGRAPHY METHOD FOR RESOLVING HETEROGENIC ANTIBODY AGGREGATES
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
EP2782925B1 (en) * 2011-11-23 2024-03-06 Sanofi Protein purification using bis-tris buffer
CN104812491B (zh) 2012-03-08 2018-06-19 生物辐射实验室股份有限公司 阴离子交换-疏水性混合模式
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
RU2501008C1 (ru) * 2012-06-14 2013-12-10 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт клинической иммунологии" Сибирского отделения Российской академии медицинских наук (ФГБУ "НИИКИ" СОРАМН) СПОСОБ АФФИННОГО ВЫДЕЛЕНИЯ АУТОАНТИТЕЛ КЛАССА IgG К ИММУНОРЕГУЛЯТОРНОМУ ЦИТОКИНУ TNF
FR2993572B1 (fr) 2012-07-23 2016-04-15 Emd Millipore Corp Circuit pour liquide biologique comportant une vanne a pincement
BR112015004467A2 (pt) 2012-09-02 2017-03-21 Abbvie Inc método para controlar a heterogeneidade de proteínas
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014034457A1 (ja) * 2012-09-03 2014-03-06 株式会社カネカ ミックスモード抗体アフィニティー分離マトリックスとそれを用いた精製方法および標的分子
BR112015005937A2 (pt) 2012-09-17 2017-07-04 Grace W R & Co meios e dispositivos cromatográficos
US11718643B2 (en) 2012-11-13 2023-08-08 Cytiva Bioprocess R&D Ab Multimodal anion exchange matrices
EP4089101A1 (en) 2013-02-26 2022-11-16 Merck Millipore Ltd Mixed-mode chromatography membranes
HK1219964A1 (zh) 2013-03-08 2017-04-21 Genzyme Corporation 治疗性蛋白药物物质的整合连续制造
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) * 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
PL2970378T3 (pl) 2013-03-15 2021-12-06 Biogen Ma Inc. Chromatografia oddziaływań hydrofobowych białek w warunkach bez soli
HUE038741T2 (hu) 2013-05-06 2018-11-28 Sanofi Sa Folyamatos többlépéses eljárás antitestek tisztítására
EP3048109A4 (en) 2013-09-17 2017-04-19 Kaneka Corporation Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN110054661A (zh) 2013-12-12 2019-07-26 Emd密理博公司 使用含丙烯酰胺的过滤器分离蛋白
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
EP3094391B1 (en) * 2014-01-17 2024-11-20 Repligen Corporation Sterilizing chromatography columns
US20240100450A9 (en) * 2014-01-17 2024-03-28 Repligen Corporation Sterilizing chromatography columns
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
CN105579463B (zh) 2014-03-14 2020-01-31 生物辐射实验室股份有限公司 混合模式配体
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
WO2016014325A1 (en) * 2014-07-21 2016-01-28 Merck Sharp & Dohme Corp. Chromatography process for purification of inlsulin and insulin analogs
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
US20170298091A1 (en) 2014-12-08 2017-10-19 Emd Millipore Corporation Mixed Bed Ion Exchange Adsorber
PL3302784T3 (pl) 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
EP4371663A3 (en) * 2016-04-06 2024-08-14 Cytiva BioProcess R&D AB Chromatography matrix
KR102369014B1 (ko) 2016-08-16 2022-03-02 리제너론 파아마슈티컬스, 인크. 혼합물로부터 개별 항체들을 정량하는 방법
ES3032778T3 (en) 2016-10-25 2025-07-24 Regeneron Pharma Methods for chromatography data analysis
CN109964123A (zh) * 2016-11-18 2019-07-02 捷恩智株式会社 抗体的纯化方法
CN110267721B (zh) 2017-01-30 2022-03-01 瑞泽恩制药公司 用于降低色谱中生物负载的组合物和方法
MY203415A (en) 2017-09-19 2024-06-27 Regeneron Pharma Methods of reducing particle formation and compositions formed thereby
US11534780B2 (en) 2017-11-14 2022-12-27 General Electric Company Spray nozzle device for delivering a restorative coating through a hole in a case of a turbine engine
US11161128B2 (en) 2017-11-14 2021-11-02 General Electric Company Spray nozzle device for delivering a restorative coating through a hole in a case of a turbine engine
EP3749697A4 (en) 2018-02-05 2021-11-03 Bio-Rad Laboratories, Inc. CHROMATOGRAPHY RESIN WITH LIGAND MIXED MODE ANIONIC / HYDROPHOBIC EXCHANGE
WO2019170635A1 (en) 2018-03-05 2019-09-12 Chiral Technologies Europe Sas Composite material for bioseparations
WO2019170634A1 (en) 2018-03-05 2019-09-12 Chiral Technologies Europe Sas Composite material for bioseparations
CN111818980B (zh) * 2018-03-08 2022-10-11 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
EP3765481A2 (en) * 2018-03-15 2021-01-20 Klawego GmbH & Co. KG Composite materials for the depletion of contaminants from solutions
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
TW202448568A (zh) 2018-07-02 2024-12-16 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
US11045773B2 (en) 2018-08-31 2021-06-29 Pall Corporation Salt tolerant porous medium
US10737259B2 (en) 2018-08-31 2020-08-11 Pall Corporation Salt tolerant anion exchange medium
AU2019332764B2 (en) 2018-08-31 2025-05-29 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
SG11202108951TA (en) * 2019-03-27 2021-09-29 Cytiva Sweden Ab A method for separating biomolecules
JP7570344B2 (ja) * 2019-03-29 2024-10-21 ブリストル－マイヤーズ スクイブ カンパニー クロマトグラフィー樹脂の疎水性を測定する方法
CN114040815B (zh) 2019-09-05 2024-06-28 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
EP4019125A1 (en) 2020-12-22 2022-06-29 Sartorius Stedim Biotech GmbH Chromatographic material and method of producing same
GB202110014D0 (en) 2021-07-12 2021-08-25 Cytiva Bioprocess R & D Ab A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof
CN118382498A (zh) 2021-12-07 2024-07-23 思拓凡生物工艺研发有限公司 用于分离靶分子的分离基质和方法
GB202202777D0 (en) 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
GB202206123D0 (en) 2022-04-27 2022-06-08 Cytiva Bioprocess R & D Ab A pre-screening method and a method for separating adeno-associated virus capsids
GB202214280D0 (en) 2022-09-29 2022-11-16 Cytiva Vioprocess R&D Ab Chromatography ligand and chromatography material, and uses thereof
GB202215814D0 (en) 2022-10-26 2022-12-07 Cytiva Bioprocess R & D Ab A chromatography device, system, and use thereof for analytic separation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2175261C2 (ru) * 1995-09-20 2001-10-27 Ново Нордиск А/С Новые аффинные лиганды и их применение
RU2389552C2 (ru) * 2004-10-21 2010-05-20 Джи-И Хелткер Байо-Сайенсиз АБ Способ очистки антител

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2405555A (en) * 1946-08-13 Manufacture of heterocyclic bases
US3467656A (en) * 1967-09-06 1969-09-16 Hoffmann La Roche 4-aryl-3,1-benzoxazine-2-thione
SE462046C (sv) 1982-08-23 1998-04-27 Pharmacia Biotech Ab Hybrid-DNA-vektor innehållande DNA-sekvens från Staphylococcus aureus, förfarande för dess framställning och bakterie innehållande densamma
US5151350A (en) * 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5322859A (en) * 1993-02-08 1994-06-21 University Of Iowa Research Foundation Antiglaucoma drug composition and method
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
SE9601368D0 (sv) * 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
JP4425994B2 (ja) * 1996-08-30 2010-03-03 ウプフロント クロマトグラフィー アクティーゼルスカブ 免疫グロブリンの単離
SE9700383D0 (sv) * 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US5908960A (en) * 1997-05-07 1999-06-01 Smithkline Beecham Corporation Compounds
CA2378519C (en) * 1999-07-07 2011-01-25 Zymogenetics, Inc. Human cytokine receptor
SE9904197D0 (sv) * 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
JP4750337B2 (ja) * 2000-06-08 2011-08-17 株式会社カネカ スルホン酸エステルの製造方法
SE0004932D0 (sv) 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
EP1355710A4 (en) * 2001-01-05 2005-01-26 Pro Chem Inc DEVICES AND METHODS FOR PURIFICATION
US6652853B2 (en) * 2001-03-08 2003-11-25 Ludwig Institute For Cancer Research Method for treating cancer using A33 specific antibodies and chemotherapeutic agents
DE10155984A1 (de) 2001-11-15 2003-05-28 Boehringer Ingelheim Pharma Verfahren zur Reduktion des Liganden-leakage von Affinitätschromatographie-Matrices
EP1601697B1 (en) * 2003-02-28 2007-05-30 Lonza Biologics plc Antibody purification by Protein A and ion exchange chromatography
WO2005082483A1 (en) * 2004-02-27 2005-09-09 Ge Healthcare Bio-Sciences Ab A process for the purification of antibodies
ES2608005T3 (es) 2004-09-22 2017-04-05 Ge Healthcare Bioprocess R&D Ab Método para preparar una matriz cromatográfica
US9266041B2 (en) * 2004-10-21 2016-02-23 Ge Healthcare Bio-Sciences Ab Chromatography ligand
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2175261C2 (ru) * 1995-09-20 2001-10-27 Ново Нордиск А/С Новые аффинные лиганды и их применение
RU2389552C2 (ru) * 2004-10-21 2010-05-20 Джи-И Хелткер Байо-Сайенсиз АБ Способ очистки антител

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HABTEMARIAM A; ET AL, Controil oa aminophosphine chelate ring-opening in Pt(II) and Pd(II) complex potential dualmode anticancer agents, J. Chem. Soc., Dalton. Trans., 2001, 1306-1318. *

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