JP4831436B2 - クロマトグラフィーリガンド - Google Patents

クロマトグラフィーリガンド Download PDF

Info

Publication number
JP4831436B2
JP4831436B2 JP2007537851A JP2007537851A JP4831436B2 JP 4831436 B2 JP4831436 B2 JP 4831436B2 JP 2007537851 A JP2007537851 A JP 2007537851A JP 2007537851 A JP2007537851 A JP 2007537851A JP 4831436 B2 JP4831436 B2 JP 4831436B2
Authority
JP
Japan
Prior art keywords
matrix
ligand
antibody
protein
separation matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2007537851A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008518885A (ja
JP2008518885A5 (enExample
Inventor
イングストランド，カリーナ
フォース，アニカ
グラッド，ガンナー
ヨハンソン，ボー−レナート
ヨハンソン，ハンス・ジェイ
マロワゼル，ジャン−リュック
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0402558A external-priority patent/SE0402558D0/xx
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2008518885A publication Critical patent/JP2008518885A/ja
Publication of JP2008518885A5 publication Critical patent/JP2008518885A5/ja
Application granted granted Critical
Publication of JP4831436B2 publication Critical patent/JP4831436B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04—Processes using organic exchangers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2007537851A 2004-10-21 2005-10-21 クロマトグラフィーリガンド Expired - Lifetime JP4831436B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0402558A SE0402558D0 (sv) 2004-10-21 2004-10-21 A process for the purification of antibodies
SE0402558-1 2004-10-21
SE0402910-4 2004-11-26
SE0402910 2004-11-26
PCT/SE2005/001592 WO2006043896A1 (en) 2004-10-21 2005-10-21 Chromatography ligand

Publications (3)

Publication Number Publication Date
JP2008518885A JP2008518885A (ja) 2008-06-05
JP2008518885A5 JP2008518885A5 (enExample) 2008-11-27
JP4831436B2 true JP4831436B2 (ja) 2011-12-07

Family

ID=36203226

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2007537851A Expired - Lifetime JP4831436B2 (ja) 2004-10-21 2005-10-21 クロマトグラフィーリガンド
JP2007537850A Expired - Lifetime JP5064225B2 (ja) 2004-10-21 2005-10-21 抗体精製法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2007537850A Expired - Lifetime JP5064225B2 (ja) 2004-10-21 2005-10-21 抗体精製法

Country Status (12)

Country Link
US (5) US7714112B2 (enExample)
EP (2) EP1827691B1 (enExample)
JP (2) JP4831436B2 (enExample)
KR (2) KR101243425B1 (enExample)
AU (2) AU2005296352B2 (enExample)
BR (2) BRPI0518207B1 (enExample)
CA (2) CA2582113C (enExample)
DK (1) DK1827691T3 (enExample)
ES (1) ES2612572T3 (enExample)
PL (1) PL1827691T3 (enExample)
RU (3) RU2389552C2 (enExample)
WO (2) WO2006043895A1 (enExample)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005073711A2 (en) 2004-01-20 2005-08-11 Pall Corporation Chromatographic material for the absorption of proteins at physiological ionic strength
JP4831436B2 (ja) 2004-10-21 2011-12-07 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィーリガンド
US9910341B2 (en) 2005-01-31 2018-03-06 The Invention Science Fund I, Llc Shared image device designation
US9489717B2 (en) 2005-01-31 2016-11-08 Invention Science Fund I, Llc Shared image device
US8606383B2 (en) 2005-01-31 2013-12-10 The Invention Science Fund I, Llc Audio sharing
US8902320B2 (en) 2005-01-31 2014-12-02 The Invention Science Fund I, Llc Shared image device synchronization or designation
US20060170956A1 (en) 2005-01-31 2006-08-03 Jung Edward K Shared image devices
US9325781B2 (en) 2005-01-31 2016-04-26 Invention Science Fund I, Llc Audio sharing
US9082456B2 (en) 2005-01-31 2015-07-14 The Invention Science Fund I Llc Shared image device designation
US20060174203A1 (en) 2005-01-31 2006-08-03 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Viewfinder for shared image device
US9124729B2 (en) 2005-01-31 2015-09-01 The Invention Science Fund I, Llc Shared image device synchronization or designation
TW200639190A (en) * 2005-02-04 2006-11-16 Showa Denko Kk Packing material for ion chromatography
US9967424B2 (en) 2005-06-02 2018-05-08 Invention Science Fund I, Llc Data storage usage protocol
US9001215B2 (en) 2005-06-02 2015-04-07 The Invention Science Fund I, Llc Estimating shared image device operational capabilities or resources
US9942511B2 (en) 2005-10-31 2018-04-10 Invention Science Fund I, Llc Preservation/degradation of video/audio aspects of a data stream
US8681225B2 (en) 2005-06-02 2014-03-25 Royce A. Levien Storage access technique for captured data
US9819490B2 (en) 2005-05-04 2017-11-14 Invention Science Fund I, Llc Regional proximity for shared image device(s)
US10003762B2 (en) 2005-04-26 2018-06-19 Invention Science Fund I, Llc Shared image devices
US9451200B2 (en) 2005-06-02 2016-09-20 Invention Science Fund I, Llc Storage access technique for captured data
US9093121B2 (en) 2006-02-28 2015-07-28 The Invention Science Fund I, Llc Data management of an audio data stream
NZ611859A (en) 2006-04-05 2014-12-24 Abbvie Biotechnology Ltd Antibody purification
US9375499B2 (en) 2006-07-14 2016-06-28 Wisconsin Alumni Research Foundation Adsorptive membranes for trapping viruses
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US8092683B2 (en) * 2007-01-10 2012-01-10 Ge Healthcare Bio-Sciences Ab Multi-modal ion exchange chromatography resins
AU2008279841B2 (en) 2007-07-25 2013-05-02 Cytiva Bioprocess R&D Ab Separation matrix
KR101163307B1 (ko) * 2007-10-26 2012-07-05 아사히 가세이 케미칼즈 가부시키가이샤 단백질의 정제 방법
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
EP2265129B1 (en) * 2008-04-08 2015-10-28 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
DE102008018734B4 (de) * 2008-04-14 2013-03-21 Sartorius Stedim Biotech Gmbh Hydrophobe Cellulose-Membran, Verfahren zu ihrer Herstellung und ihre Verwendung in der hydrophoben Interaktionschromatographie
DE102008018732B4 (de) * 2008-04-14 2022-06-09 Sartorius Stedim Biotech Gmbh Verfahren zur Stofftrennung unter Verwendung einer Cellulosehydrat-Membran in der Größenausschlusschromatographie
DE102008055821A1 (de) * 2008-04-14 2009-10-15 Sartorius Stedim Biotech Gmbh Cellulosehydrat-Membran, Verfahren zur ihrer Herstellung und Verwendung davon
FR2931838B1 (fr) 2008-06-02 2010-06-11 Millipore Corp Installation pour traiter un liquide biologique.
WO2010030222A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal with multimodal anion exchangers in the presence of protein-excluded zwitterions
WO2010071208A1 (ja) * 2008-12-19 2010-06-24 武田薬品工業株式会社 抗体の精製方法
FR2940145B1 (fr) 2008-12-24 2011-03-25 Millipore Corp Chariot et installation de traitement d'un liquide biologique
JP2010158624A (ja) * 2009-01-08 2010-07-22 Asahi Kasei Chemicals Corp 多孔質吸着膜、及び当該多孔質吸着膜を用いたたんぱく質の精製方法
FR2941385B1 (fr) 2009-01-23 2011-04-01 Millipore Corp Procede pour fournir un circuit pour liquide biologique et circuit obtenu.
US9441011B2 (en) 2009-07-03 2016-09-13 Asahi Kasei Chemicals Corporation Method for purification of antibody using porous membrane having amino group and alkyl group both bound to graft chain immobilized on porous substrate
EP2459308A1 (en) 2009-07-28 2012-06-06 instrAction GmbH Specific sorbent for binding proteins and peptides, and separation method using the same
US8277649B2 (en) 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
FR2955119B1 (fr) 2010-01-13 2012-12-28 Millipore Corp Circuit pour liquide biologique
EP4492053A3 (en) 2010-05-25 2025-03-19 F. Hoffmann-La Roche AG Methods of purifying polypeptides
FR2960796B1 (fr) 2010-06-08 2014-01-24 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2960795B1 (fr) 2010-06-08 2012-07-27 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2960794B1 (fr) 2010-06-08 2012-07-27 Millipore Corp Dispositif pour une installation de traitement de liquide biologique
FR2961713B1 (fr) 2010-06-23 2012-08-10 Millipore Corp Poche pour circuit d'une installation de traitement de liquide biologique
FR2961711B1 (fr) 2010-06-23 2012-08-17 Millipore Corp Poche pour circuit d'une installation de traitement de liquide biologique
KR101997543B1 (ko) 2010-07-30 2019-07-09 이엠디 밀리포어 코포레이션 크로마토그래피 매질 및 방법
FR2963573B1 (fr) 2010-08-03 2012-08-31 Millipore Corp Chariot de pompage pour une installation de traitement de liquide biologique
MX2013003182A (es) * 2010-09-20 2013-04-24 Abbvie Inc Purificacion de anticuerpos por cromatografia de lecho movil simulada.
EP2655412B1 (en) * 2010-12-21 2018-01-17 F. Hoffmann-La Roche AG Isoform enriched antibody preparation and method for obtaining it
FR2973396B1 (fr) 2011-03-28 2013-05-10 Millipore Corp Installation de traitement de liquide biologique
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2570184A1 (en) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbent comprising on its surface an aromatic ring system having an anionic or deprotonizable group for the purification of organic molecules
EP2570185A1 (en) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbent comprising an aromatic ring system on its surface for the purification of organic molecules
US8802448B2 (en) 2011-07-27 2014-08-12 Pall Corporation Mixed mode ligands
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
DK2768857T3 (da) * 2011-10-19 2020-02-03 Novimmune Sa Fremgangsmåder til oprensning af antistoffer
US9309282B2 (en) 2011-10-19 2016-04-12 Bio-Rad Laboratories, Inc. Solid phase for mixed-mode chromatographic purification of proteins
EP2773439A4 (en) * 2011-10-31 2015-07-01 Merck Sharp & Dohme CHROMATOGRAPHY METHOD FOR RESOLVING HETEROGENIC ANTIBODY AGGREGATES
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
EP2782925B1 (en) * 2011-11-23 2024-03-06 Sanofi Protein purification using bis-tris buffer
CN104812491B (zh) 2012-03-08 2018-06-19 生物辐射实验室股份有限公司 阴离子交换-疏水性混合模式
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
RU2501008C1 (ru) * 2012-06-14 2013-12-10 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт клинической иммунологии" Сибирского отделения Российской академии медицинских наук (ФГБУ "НИИКИ" СОРАМН) СПОСОБ АФФИННОГО ВЫДЕЛЕНИЯ АУТОАНТИТЕЛ КЛАССА IgG К ИММУНОРЕГУЛЯТОРНОМУ ЦИТОКИНУ TNF
FR2993572B1 (fr) 2012-07-23 2016-04-15 Emd Millipore Corp Circuit pour liquide biologique comportant une vanne a pincement
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201501597VA (en) * 2012-09-03 2015-04-29 Kaneka Corp Mix-mode antibody affinity separation matrix and purification method using same, and target molecule
SG11201404681VA (en) 2012-09-17 2014-09-26 Grace W R & Co Chromatography media and devices
WO2014077762A1 (en) 2012-11-13 2014-05-22 Ge Healthcare Bio-Sciences Ab Multimodal anion exchange matrices
JP6639236B2 (ja) 2013-02-26 2020-02-05 ナトリックス セパレイションズ インコーポレーテッド 混合モードクロマトグラフィー膜
IL274646B (en) 2013-03-08 2022-09-01 Genzyme Corp Integrated continuous production of medicinal substances from medical protein
EP2830651A4 (en) 2013-03-12 2015-09-02 Abbvie Inc HUMAN ANTIBODIES THAT BIND TNF-ALPHA AND PREPARATION METHODS
US10023608B1 (en) * 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9994609B2 (en) 2013-03-15 2018-06-12 Biogen Ma Inc. Hydrophobic interaction protein chromatography under no-salt conditions
TWI631132B (zh) * 2013-05-06 2018-08-01 賽諾菲公司 用於純化抗體之連續多步驟方法
JPWO2015041218A1 (ja) 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
EP3698870A1 (en) 2013-12-12 2020-08-26 EMD Millipore Corporation Protein separations using an acrylamide containing filter
SG11201605712SA (en) 2014-01-16 2016-08-30 Grace W R & Co Affinity chromatography media and chromatography devices
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) * 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
EP3094391B1 (en) * 2014-01-17 2024-11-20 Repligen Corporation Sterilizing chromatography columns
US20240100450A9 (en) * 2014-01-17 2024-03-28 Repligen Corporation Sterilizing chromatography columns
WO2015138928A2 (en) 2014-03-14 2015-09-17 Bio-Rad Laboratories, Inc. Mixed mode ligands
CN107847907A (zh) 2014-05-02 2018-03-27 格雷斯公司 官能化载体材料以及制备和使用官能化载体材料的方法
US10155799B2 (en) * 2014-07-21 2018-12-18 Merck Sharp & Dohme Corp. Chromatography process for purification of insulin and insulin analogs
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
CA2966515C (en) 2014-12-08 2021-04-27 Emd Millipore Corporation Mixed bed ion exchange adsorber
US10695744B2 (en) 2015-06-05 2020-06-30 W. R. Grace & Co.-Conn. Adsorbent biprocessing clarification agents and methods of making and using the same
WO2017174422A1 (en) * 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
US11020686B2 (en) 2016-08-16 2021-06-01 Regeneron Pharmaceuticals, Inc. Methods for quantitating individual antibodies from a mixture
BR112019006689A2 (pt) 2016-10-25 2019-06-25 Regeneron Pharma métodos e sistemas para análise de dados de cromatografia
CN109964123A (zh) * 2016-11-18 2019-07-02 捷恩智株式会社 抗体的纯化方法
KR20240152951A (ko) 2017-01-30 2024-10-22 리제너론 파마슈티칼스 인코포레이티드 크로마토그래피에서 바이오버든을 감소시키기 위한 조성물 및 방법
US12042538B2 (en) 2017-09-19 2024-07-23 Regeneron Pharmaceuticals, Inc. Methods of reducing particle formation and compositions formed thereby
US11534780B2 (en) 2017-11-14 2022-12-27 General Electric Company Spray nozzle device for delivering a restorative coating through a hole in a case of a turbine engine
US11161128B2 (en) 2017-11-14 2021-11-02 General Electric Company Spray nozzle device for delivering a restorative coating through a hole in a case of a turbine engine
CN111683976B (zh) 2018-02-05 2022-11-18 生物辐射实验室股份有限公司 具有阴离子交换-疏水混合模式配体的色谱树脂
WO2019170635A1 (en) 2018-03-05 2019-09-12 Chiral Technologies Europe Sas Composite material for bioseparations
CN111818981B (zh) 2018-03-05 2022-07-05 欧洲手性技术股份公司 用于生物分离的复合材料
CN111818980B (zh) 2018-03-08 2022-10-11 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
EP3765481A2 (en) * 2018-03-15 2021-01-20 Klawego GmbH & Co. KG Composite materials for the depletion of contaminants from solutions
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
US10737259B2 (en) 2018-08-31 2020-08-11 Pall Corporation Salt tolerant anion exchange medium
US11045773B2 (en) 2018-08-31 2021-06-29 Pall Corporation Salt tolerant porous medium
EP3843869A1 (en) 2018-08-31 2021-07-07 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
WO2020193483A1 (en) 2019-03-27 2020-10-01 Cytiva Sweden Ab A method for separating biomolecules
CN113646630B (zh) * 2019-03-29 2024-05-07 百时美施贵宝公司 测量色谱树脂的疏水性的方法
CN114040815B (zh) 2019-09-05 2024-06-28 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
EP4019125A1 (en) 2020-12-22 2022-06-29 Sartorius Stedim Biotech GmbH Chromatographic material and method of producing same
GB202110014D0 (en) 2021-07-12 2021-08-25 Cytiva Bioprocess R & D Ab A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof
WO2023104770A1 (en) 2021-12-07 2023-06-15 Cytiva Bioprocess R&D Ab Separation matrix and methods for separating target molecules
GB202202777D0 (en) 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
GB202206123D0 (en) 2022-04-27 2022-06-08 Cytiva Bioprocess R & D Ab A pre-screening method and a method for separating adeno-associated virus capsids
GB202214280D0 (en) 2022-09-29 2022-11-16 Cytiva Vioprocess R&D Ab Chromatography ligand and chromatography material, and uses thereof
GB202215814D0 (en) 2022-10-26 2022-12-07 Cytiva Bioprocess R & D Ab A chromatography device, system, and use thereof for analytic separation

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2405555A (en) * 1946-08-13 Manufacture of heterocyclic bases
US3467656A (en) * 1967-09-06 1969-09-16 Hoffmann La Roche 4-aryl-3,1-benzoxazine-2-thione
SE462046C (sv) 1982-08-23 1998-04-27 Pharmacia Biotech Ab Hybrid-DNA-vektor innehållande DNA-sekvens från Staphylococcus aureus, förfarande för dess framställning och bakterie innehållande densamma
US5151350A (en) * 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5322859A (en) * 1993-02-08 1994-06-21 University Of Iowa Research Foundation Antiglaucoma drug composition and method
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
SE9601368D0 (sv) * 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
AU729039C (en) * 1996-08-30 2001-08-02 Upfront Chromatography A/S Isolation of immunoglobulins
SE9700383D0 (sv) * 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US5908960A (en) * 1997-05-07 1999-06-01 Smithkline Beecham Corporation Compounds
ES2364086T3 (es) * 1999-07-07 2011-08-24 Zymogenetics, Inc. Receptor de citoquina humana.
SE9904197D0 (sv) * 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
EP1288198B1 (en) * 2000-06-08 2006-12-20 Kaneka Corporation Process for the production of sulfonic esters
SE0004932D0 (sv) 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
WO2002053256A1 (en) * 2001-01-05 2002-07-11 Pro-Chem, Inc. Devices and methods for purification
US6652853B2 (en) * 2001-03-08 2003-11-25 Ludwig Institute For Cancer Research Method for treating cancer using A33 specific antibodies and chemotherapeutic agents
DE10155984A1 (de) 2001-11-15 2003-05-28 Boehringer Ingelheim Pharma Verfahren zur Reduktion des Liganden-leakage von Affinitätschromatographie-Matrices
DK1601697T3 (da) * 2003-02-28 2007-10-01 Lonza Biologics Plc Oprensning af antistof ved protein A- og ionbytningskromatografi
CA2552639C (en) * 2004-02-27 2012-05-01 Ge Healthcare Bio-Sciences Ab A process for the purification of antibodies
EP1793926B1 (en) 2004-09-22 2016-11-23 GE Healthcare BioProcess R&D AB Method of preparing a chromatography matrix
US9266041B2 (en) * 2004-10-21 2016-02-23 Ge Healthcare Bio-Sciences Ab Chromatography ligand
JP4831436B2 (ja) * 2004-10-21 2011-12-07 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィーリガンド
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers

Also Published As

Publication number Publication date
US10751645B2 (en) 2020-08-25
AU2005296351B2 (en) 2010-12-02
EP1827691A1 (en) 2007-09-05
CA2582113A1 (en) 2006-04-27
BRPI0518207A (pt) 2008-11-04
US20070259453A1 (en) 2007-11-08
PL1827691T3 (pl) 2017-07-31
WO2006043895A1 (en) 2006-04-27
ES2612572T3 (es) 2017-05-17
EP1827691B1 (en) 2016-12-07
KR20070072532A (ko) 2007-07-04
RU2541429C2 (ru) 2015-02-10
RU2007112990A (ru) 2008-11-27
RU2396246C2 (ru) 2010-08-10
JP2008518885A (ja) 2008-06-05
US20180154281A1 (en) 2018-06-07
US20110118442A1 (en) 2011-05-19
AU2005296351A1 (en) 2006-04-27
CA2583578A1 (en) 2006-04-27
BRPI0518207B1 (pt) 2023-05-16
BRPI0518216C1 (enExample) 2017-03-21
BRPI0518216B8 (pt) 2016-10-25
JP2008517906A (ja) 2008-05-29
WO2006043896A1 (en) 2006-04-27
DK1827691T3 (en) 2017-02-13
JP5064225B2 (ja) 2012-10-31
RU2010110437A (ru) 2011-09-27
US8895710B2 (en) 2014-11-25
CA2583578C (en) 2014-04-01
RU2007112991A (ru) 2008-11-27
EP1807205B1 (en) 2012-09-26
KR20070067159A (ko) 2007-06-27
KR101243601B1 (ko) 2013-03-20
BRPI0518216A (pt) 2008-11-04
EP1807205A1 (en) 2007-07-18
US9878266B2 (en) 2018-01-30
RU2389552C2 (ru) 2010-05-20
US20070244307A1 (en) 2007-10-18
CA2582113C (en) 2013-07-30
US7714112B2 (en) 2010-05-11
AU2005296352B2 (en) 2011-01-06
US7867784B2 (en) 2011-01-11
BRPI0518216C8 (pt) 2017-03-21
KR101243425B1 (ko) 2013-03-13
AU2005296352A1 (en) 2006-04-27
US20150073128A1 (en) 2015-03-12
BRPI0518216B1 (pt) 2016-04-05

Similar Documents

Publication Publication Date Title
JP4831436B2 (ja) クロマトグラフィーリガンド
JP4776615B2 (ja) 抗体精製
JP4848356B2 (ja) 抗体精製法
US9266041B2 (en) Chromatography ligand
CN101060931B (zh) 色谱配体

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20081007

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20081007

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20110114

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20110114

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110419

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110705

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110809

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110907

R150 Certificate of patent or registration of utility model

Ref document number: 4831436

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140930

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250