RU2390526C2 - Процесс и аппарат для отделения белка, представляющего интерес, от гетерогенной тканевой культуральной жидкой смеси и его очистки - Google Patents
Процесс и аппарат для отделения белка, представляющего интерес, от гетерогенной тканевой культуральной жидкой смеси и его очистки Download PDFInfo
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- RU2390526C2 RU2390526C2 RU2007115901/13A RU2007115901A RU2390526C2 RU 2390526 C2 RU2390526 C2 RU 2390526C2 RU 2007115901/13 A RU2007115901/13 A RU 2007115901/13A RU 2007115901 A RU2007115901 A RU 2007115901A RU 2390526 C2 RU2390526 C2 RU 2390526C2
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2679133C2 (ru) * | 2014-01-17 | 2019-02-06 | Джензим Корпорейшн | Способы стерильной хроматографии и производства |
| US10711034B2 (en) | 2013-03-08 | 2020-07-14 | Genzyme Corporation | Integrated continuous manufacturing of therapeutic protein drug substances |
| US10919021B2 (en) | 2014-01-17 | 2021-02-16 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| US11369703B2 (en) | 2018-08-31 | 2022-06-28 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| RU2786078C2 (ru) * | 2014-01-17 | 2022-12-16 | Джензим Корпорейшн | Способы стерильной хроматографии и производства |
Families Citing this family (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101238798B1 (ko) | 2004-09-30 | 2013-03-04 | 바이엘 헬스케어, 엘엘씨 | 생물학적 분자의 통합된 연속 제조를 위한 장치 및 방법 |
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| FR2920429B1 (fr) * | 2007-08-30 | 2012-10-05 | Lfb Biotechnologies | Procede de purification du facteur viii et du facteur von willebrand |
| KR20110091678A (ko) * | 2008-10-20 | 2011-08-12 | 아보트 러보러터리즈 | 단백질 ａ 친화성 크로마토그래피를 이용한 항체의 분리 및 정제 |
| NZ592097A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Viral inactivation during purification of il-12 and il-18 antibodies |
| US9527010B2 (en) | 2009-09-25 | 2016-12-27 | Ge Healthcare Bio-Sciences Corp. | Separation system and method |
| CN103298542A (zh) | 2010-10-15 | 2013-09-11 | 艾克塞勒雷克斯公司 | 大容量的一次性超滤系统和方法 |
| CN102154408B (zh) * | 2011-01-13 | 2013-06-05 | 天津市工业微生物研究所 | 小核菌硬葡聚糖在线发酵提取方法及系统 |
| US12227734B2 (en) | 2011-03-08 | 2025-02-18 | University Of Maryland, Baltimore County | Microscale bioprocessing system and method for protein manufacturing from human blood |
| WO2012122413A1 (en) * | 2011-03-08 | 2012-09-13 | University Of Maryland Baltimore County | Microscale bioprocessing system and method for protein manufacturing |
| US10774304B2 (en) * | 2011-03-08 | 2020-09-15 | University Of Maryland, Baltimore County | System and method for production of on-demand proteins in a portable unit for point of care delivery |
| US9982227B2 (en) | 2011-03-08 | 2018-05-29 | University Of Maryland, Baltimore County | System and method for production of on-demand proteins in a portable unit for point of care delivery |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| CN104025408A (zh) * | 2011-10-11 | 2014-09-03 | 森春减压系统有限公司 | 用于电力变压器的减压系统 |
| CN104160013B (zh) | 2012-01-18 | 2017-07-28 | 拜尔健康护理有限责任公司 | 灌流式生物反应器系统及操作其的方法 |
| US9997264B2 (en) * | 2012-02-14 | 2018-06-12 | Control Components, Inc. | Enhanced nuclear sump strainer system |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| BR112015004467A2 (pt) | 2012-09-02 | 2017-03-21 | Abbvie Inc | método para controlar a heterogeneidade de proteínas |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| MX369522B (es) | 2013-02-22 | 2019-11-11 | Genzyme Corp | Metodos de cultivo por perfusión con microportadores y usos de los mismos. |
| SG11201506343QA (en) | 2013-02-22 | 2015-09-29 | Genzyme Corp | Microcarrier perfusion culturing methods and uses thereof |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| CN106661083A (zh) * | 2014-05-02 | 2017-05-10 | 诺和诺德股份有限公司 | 集成的连续生物制造处理方法 |
| MX383879B (es) * | 2014-05-13 | 2025-03-14 | Amgen Inc | Sistema y métodos de control del proceso para usar con filtros y procesos de filtración. |
| US9975066B2 (en) * | 2014-05-27 | 2018-05-22 | Artificial Cell Technologies, Inc. | Automated layer by layer construction of multilayer coated cores by TFF |
| TWI743024B (zh) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
| TWI776235B (zh) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| US10550148B2 (en) | 2014-06-16 | 2020-02-04 | Emd Millipore Corporation | Methods for increasing the capacity of flow-through processes |
| WO2015195452A2 (en) | 2014-06-16 | 2015-12-23 | Emd Millipore Corporation | Single-pass filtration systems and processes |
| SG11201508171RA (en) | 2014-06-25 | 2016-01-28 | Emd Millipore Corp | Compact spiral-wound filter elements, modules and systems |
| US10350518B2 (en) | 2014-08-29 | 2019-07-16 | Emd Millipore Corporation | Processes for filtering liquids using single pass tangential flow filtration systems and tangential flow filtration systems with recirculation of retentate |
| KR102188947B1 (ko) | 2014-08-29 | 2020-12-09 | 이엠디 밀리포어 코포레이션 | 잔류물의 재순환을 이용한 싱글 패스 접선 유동 여과 시스템 및 접선 유동 여과 시스템 |
| USD761381S1 (en) | 2014-12-18 | 2016-07-12 | Emd Millipore Corporation | Retentate plate |
| USD762811S1 (en) | 2014-12-18 | 2016-08-02 | Emd Millipore Corporation | Diverter plate |
| EP3093335A1 (de) | 2015-05-13 | 2016-11-16 | Bayer Technology Services GmbH | Prozessleitsystem zur regelung und steuerung einer modular aufgebauten anlage zur produktion von biopharmazeutischen und biologischen makromolekularen produkten |
| ITUA20161506A1 (it) * | 2016-03-09 | 2017-09-09 | Della Toffola Spa | Metodo ed impianto perfezionato di filtrazione tangenziale |
| WO2017160739A1 (en) | 2016-03-14 | 2017-09-21 | Pendo TECH | Processing system for multiple tangential flow filtration stations in bioprocessing applications |
| EP4066927B1 (en) | 2016-06-09 | 2024-10-09 | EMD Millipore Corporation | Methods of using radial-path filter elements |
| JP7082453B2 (ja) * | 2016-07-21 | 2022-06-08 | エージェンシー フォー サイエンス，テクノロジー アンド リサーチ | 高体積分率粒子精密濾過のための外壁集束のための装置及びその製造方法 |
| AU2017358509A1 (en) * | 2016-11-11 | 2019-05-09 | Bayer Aktiengesellschaft | Method for sampling fluid streams for monitoring contaminants in a continuous flow |
| EP3141594A3 (en) * | 2016-11-11 | 2017-06-14 | Bayer Pharma Aktiengesellschaft | Method for sampling fluid streams for monitoring contaminants in a continuous flow |
| WO2018107099A1 (en) * | 2016-12-09 | 2018-06-14 | Lonza Ltd | Method for the reduction of viral titer in pharmaceuticals |
| EP3354330A1 (en) * | 2017-01-25 | 2018-08-01 | Sartorius Stedim Biotech GmbH | Automated modular filtration system |
| CA2994643A1 (en) * | 2017-02-14 | 2018-08-14 | Bart Lipkens | Bioreactor using acoustic standing waves |
| WO2018159847A1 (ja) * | 2017-03-03 | 2018-09-07 | 富士フイルム株式会社 | 細胞培養装置及び細胞培養方法 |
| JP2018153168A (ja) * | 2017-03-21 | 2018-10-04 | 国立大学法人九州大学 | 微生物収集装置 |
| DE102017110482A1 (de) * | 2017-05-15 | 2018-11-15 | Sartorius Stedim Biotech Gmbh | Filtrationsmodul bzw. Reinigungsmodul für modulares Filtrationssystem |
| DE102017111133A1 (de) | 2017-05-22 | 2018-11-22 | Sartorius Stedim Biotech Gmbh | Vorkonfigurierte Einweg-Filtrationsvorrichtung |
| TWI675696B (zh) * | 2017-06-01 | 2019-11-01 | 美商Ｅｍｄ密理博公司 | 用於灌注應用之切向流過濾裝置 |
| US11364469B2 (en) | 2017-06-20 | 2022-06-21 | Asahi Kasei Kabushiki Kaisha | Hollow fiber membrane module and filtering method |
| TWI846694B (zh) * | 2018-05-04 | 2024-07-01 | 美商健臻公司 | 具有過濾系統的灌注式生物反應器 |
| US12071607B2 (en) * | 2018-06-01 | 2024-08-27 | Lonza Ltd. | Midscale model for organic growth and phasing |
| GB201810772D0 (en) * | 2018-06-29 | 2018-08-15 | Ge Healthcare Bio Sciences Ab | Method in bioprocess purification system |
| GB201816871D0 (en) | 2018-10-17 | 2018-11-28 | Ge Healthcare Bio Sciences Ab | A bioprocessing fluid sensor arrangement |
| DE102019100209A1 (de) | 2019-01-07 | 2020-07-09 | Sartorius Stedim Biotech Gmbh | Vorrichtung und Verfahren zum Ausgleich von kurzfristigen Druck- oder Volumenschwankungen eines Mediums in einem kontinuierlich geführten biopharmazeutischen Prozess |
| SG11202107441RA (en) * | 2019-02-15 | 2021-08-30 | Just Evotec Biologics Inc | Automated biomanufacturing systems, facilities, and processes |
| CN111018968B (zh) * | 2019-12-16 | 2021-07-27 | 兴盟生物医药(苏州)有限公司 | 一种通过超滤浓缩制备高浓度抗体制剂的方法 |
| US12050448B2 (en) | 2021-01-29 | 2024-07-30 | Tata Consultancy Services Limited | Method and system to control a continuous bioprocessing |
| GB202109015D0 (en) * | 2021-06-23 | 2021-08-04 | Puridify Ltd | A method for purifying a feed in a chromatography system |
| CN118984710A (zh) * | 2022-03-30 | 2024-11-19 | 富士胶片株式会社 | 生物制药的原料药的制造方法、生物制药的原料药的制造系统及生物制药的原料药 |
| CN118973592A (zh) * | 2022-03-30 | 2024-11-15 | 富士胶片株式会社 | 生物制药的原料药的制造方法、生物制药的原料药的制造系统及生物制药的原料药 |
| WO2024072963A1 (en) * | 2022-09-28 | 2024-04-04 | Genzyme Corporation | Systems and devices for producing biological products and methods of using the same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5490937A (en) * | 1990-09-17 | 1996-02-13 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| US6350382B1 (en) * | 2000-06-23 | 2002-02-26 | Scilog, Inc. | Enhancing filtration yields in tangential flow filtration |
| US20040139864A1 (en) * | 2001-09-10 | 2004-07-22 | Kopf Henry B. | Method and apparatus for separation of milk, colostrum, and whey |
| US20040167320A1 (en) * | 2003-02-24 | 2004-08-26 | Couto Daniel E. | Methods of tangential flow filtration and an apparatus therefore |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5157113A (en) * | 1987-08-10 | 1992-10-20 | Miles Inc. | Removal of nucleic acids from monoclonal antibody preparations |
| US5463029A (en) | 1992-11-23 | 1995-10-31 | Immunex Corporation | Purification of fusion proteins comprising GM-CSF and IL-3 |
| BR9507657A (pt) * | 1994-05-16 | 1997-09-09 | Merck & Co Inc | Proteina isolada e purificada de papilomavirus recombinante capsideos particulas semelhantes a virus processos a virus processos para produzir a proteina para tratar ou prevenir a infecçao por papilomavirus para produzir uma proteina de capsideo de papilomavirus recombinante montada em um capsideo ou particula semelhante a virus e para induzir uma resposta imune em um animal vertebrado composiçoes farmacêuticas kit para a detecçao de papilomavirus ou anticorpos para papilomavirus e sistema sinte |
| US5616595A (en) * | 1995-06-07 | 1997-04-01 | Abbott Laboratories | Process for recovering water insoluble compounds from a fermentation broth |
| JP3566461B2 (ja) | 1996-06-28 | 2004-09-15 | ユニ・チャーム株式会社 | 使い捨ての体液吸収性物品 |
| US7122344B2 (en) | 1997-05-28 | 2006-10-17 | Claragen, Inc. | Methods for the production of purified recombinant human uteroglobin for the treatment of inflammatory and fibrotic conditions |
| DE19837391A1 (de) * | 1997-08-22 | 1999-02-25 | Hoffmann La Roche | Immunologische Materialien und Verfahren zum Nachweis von Dihydropyrimidindehydrogenase |
| US6022477A (en) * | 1997-11-14 | 2000-02-08 | New Jersey Institute Of Technology | Method and apparatus for isolation purification of biomolecules |
| US7820796B2 (en) | 1998-03-12 | 2010-10-26 | Genetics Institute, Llc. | Methods for producing Factor VIII proteins |
| US6444223B1 (en) * | 1999-05-28 | 2002-09-03 | Alkermes Controlled Therapeutics, Inc. | Method of producing submicron particles of a labile agent and use thereof |
| US6544424B1 (en) * | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| JP2005520484A (ja) * | 2001-07-06 | 2005-07-14 | ４５４ コーポレイション | 多孔性フィルターを使用し、独立した並行する化学的微量反応を隔離するための方法 |
| DE60238863D1 (de) * | 2001-08-31 | 2011-02-17 | Bayer Schering Pharma Ag | Vorrichtung und verfahren zur fermentation in hohen zelldichten |
| US7078507B2 (en) * | 2001-11-09 | 2006-07-18 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Synthetic genes for malarial proteins and methods of use |
| WO2004072292A1 (fr) * | 2003-02-11 | 2004-08-26 | Centre National En Electrochimie Et En Technologies Environnementales Inc. | Procede et dispositif pour la production de biomolecules en continu |
| US20050197496A1 (en) * | 2004-03-04 | 2005-09-08 | Gtc Biotherapeutics, Inc. | Methods of protein fractionation using high performance tangential flow filtration |
| KR101238798B1 (ko) | 2004-09-30 | 2013-03-04 | 바이엘 헬스케어, 엘엘씨 | 생물학적 분자의 통합된 연속 제조를 위한 장치 및 방법 |
-
2005
- 2005-09-30 KR KR1020077008684A patent/KR101238798B1/ko not_active Expired - Lifetime
- 2005-09-30 UA UAA201202077A patent/UA107937C2/ru unknown
- 2005-09-30 KR KR1020127030406A patent/KR101335936B1/ko not_active Expired - Lifetime
- 2005-09-30 HU HUE12179336A patent/HUE032538T2/en unknown
- 2005-09-30 EP EP05800224.7A patent/EP1807101B1/en not_active Expired - Lifetime
- 2005-09-30 US US11/576,097 patent/US9045725B2/en active Active
- 2005-09-30 CA CA2579845A patent/CA2579845C/en not_active Expired - Lifetime
- 2005-09-30 WO PCT/US2005/035364 patent/WO2006039588A2/en not_active Ceased
- 2005-09-30 ES ES05800224.7T patent/ES2570006T3/es not_active Expired - Lifetime
- 2005-09-30 SI SI200532141T patent/SI2550971T1/sl unknown
- 2005-09-30 PT PT121793368T patent/PT2550971T/pt unknown
- 2005-09-30 DK DK12179336.8T patent/DK2550971T3/en active
- 2005-09-30 DK DK05800224.7T patent/DK1807101T3/en active
- 2005-09-30 PL PL05800224.7T patent/PL1807101T3/pl unknown
- 2005-09-30 AU AU2005292358A patent/AU2005292358B2/en not_active Ceased
- 2005-09-30 HU HUE05800224A patent/HUE027136T2/en unknown
- 2005-09-30 NZ NZ554307A patent/NZ554307A/en not_active IP Right Cessation
- 2005-09-30 UA UAA201304498A patent/UA112529C2/uk unknown
- 2005-09-30 EP EP12179336.8A patent/EP2550971B1/en not_active Expired - Lifetime
- 2005-09-30 UA UAA200704547A patent/UA99249C2/ru unknown
- 2005-09-30 HR HRP20070097AA patent/HRP20070097B1/hr not_active IP Right Cessation
- 2005-09-30 CN CN201210289781.1A patent/CN102794109B/zh not_active Expired - Lifetime
- 2005-09-30 ES ES12179336.8T patent/ES2634323T3/es not_active Expired - Lifetime
- 2005-09-30 LT LTEP12179336.8T patent/LT2550971T/lt unknown
- 2005-09-30 RU RU2007115901/13A patent/RU2390526C2/ru active
- 2005-09-30 CN CN2005800331770A patent/CN101076347B/zh not_active Expired - Lifetime
- 2005-09-30 JP JP2007534832A patent/JP5579966B2/ja not_active Expired - Lifetime
- 2005-09-30 NZ NZ588616A patent/NZ588616A/en not_active IP Right Cessation
- 2005-09-30 BR BRPI0515849-4A patent/BRPI0515849B1/pt not_active IP Right Cessation
-
2007
- 2007-03-07 IL IL181775A patent/IL181775A/en active IP Right Grant
- 2007-03-28 ZA ZA2007/02578A patent/ZA200702578B/en unknown
- 2007-04-26 NO NO20072174A patent/NO341439B1/no unknown
-
2010
- 2010-04-21 IL IL205228A patent/IL205228A/en active IP Right Grant
-
2011
- 2011-12-22 IL IL217157A patent/IL217157A/en active IP Right Grant
- 2011-12-22 IL IL217158A patent/IL217158A/en active IP Right Grant
- 2011-12-22 IL IL217159A patent/IL217159A/en active IP Right Grant
-
2012
- 2012-06-07 JP JP2012129422A patent/JP5762360B2/ja not_active Expired - Lifetime
-
2013
- 2013-03-12 US US13/795,298 patent/US9133433B2/en active Active
- 2013-03-12 US US13/795,346 patent/US20130345402A1/en not_active Abandoned
-
2016
- 2016-05-11 CY CY20161100396T patent/CY1117699T1/el unknown
-
2017
- 2017-07-10 NO NO20171138A patent/NO341885B1/no unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5490937A (en) * | 1990-09-17 | 1996-02-13 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| US6350382B1 (en) * | 2000-06-23 | 2002-02-26 | Scilog, Inc. | Enhancing filtration yields in tangential flow filtration |
| US20040139864A1 (en) * | 2001-09-10 | 2004-07-22 | Kopf Henry B. | Method and apparatus for separation of milk, colostrum, and whey |
| US20040167320A1 (en) * | 2003-02-24 | 2004-08-26 | Couto Daniel E. | Methods of tangential flow filtration and an apparatus therefore |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10711034B2 (en) | 2013-03-08 | 2020-07-14 | Genzyme Corporation | Integrated continuous manufacturing of therapeutic protein drug substances |
| US12110312B2 (en) | 2013-03-08 | 2024-10-08 | Genzyme Corporation | Integrated continuous manufacturing of therapeutic protein drug substances |
| RU2679133C2 (ru) * | 2014-01-17 | 2019-02-06 | Джензим Корпорейшн | Способы стерильной хроматографии и производства |
| US10919021B2 (en) | 2014-01-17 | 2021-02-16 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| RU2786078C2 (ru) * | 2014-01-17 | 2022-12-16 | Джензим Корпорейшн | Способы стерильной хроматографии и производства |
| US11839861B2 (en) | 2014-01-17 | 2023-12-12 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| US11912739B2 (en) | 2014-01-17 | 2024-02-27 | Genzyme Corporation | Sterile chromatography and manufacturing processes |
| US11369703B2 (en) | 2018-08-31 | 2022-06-28 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
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