CN102154408B - 小核菌硬葡聚糖在线发酵提取方法及系统 - Google Patents
小核菌硬葡聚糖在线发酵提取方法及系统 Download PDFInfo
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- CN102154408B CN102154408B CN 201110006769 CN201110006769A CN102154408B CN 102154408 B CN102154408 B CN 102154408B CN 201110006769 CN201110006769 CN 201110006769 CN 201110006769 A CN201110006769 A CN 201110006769A CN 102154408 B CN102154408 B CN 102154408B
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- 238000000855 fermentation Methods 0.000 title claims abstract description 53
- 230000004151 fermentation Effects 0.000 title claims abstract description 53
- FEBUJFMRSBAMES-UHFFFAOYSA-N 2-[(2-{[3,5-dihydroxy-2-(hydroxymethyl)-6-phosphanyloxan-4-yl]oxy}-3,5-dihydroxy-6-({[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}methyl)oxan-4-yl)oxy]-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl phosphinite Chemical compound OC1C(O)C(O)C(CO)OC1OCC1C(O)C(OC2C(C(OP)C(O)C(CO)O2)O)C(O)C(OC2C(C(CO)OC(P)C2O)O)O1 FEBUJFMRSBAMES-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 229920002305 Schizophyllan Polymers 0.000 title claims abstract description 32
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- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
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- 230000003110 anti-inflammatory effect Effects 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000012262 fermentative production Methods 0.000 description 2
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- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
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- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
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- 230000000259 anti-tumor effect Effects 0.000 description 1
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- 235000012000 cholesterol Nutrition 0.000 description 1
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- 238000011033 desalting Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
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- 230000002194 synthesizing effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
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CN 201110006769 CN102154408B (zh) | 2011-01-13 | 2011-01-13 | 小核菌硬葡聚糖在线发酵提取方法及系统 |
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CN 201110006769 CN102154408B (zh) | 2011-01-13 | 2011-01-13 | 小核菌硬葡聚糖在线发酵提取方法及系统 |
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CN102154408A CN102154408A (zh) | 2011-08-17 |
CN102154408B true CN102154408B (zh) | 2013-06-05 |
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CN 201110006769 Active CN102154408B (zh) | 2011-01-13 | 2011-01-13 | 小核菌硬葡聚糖在线发酵提取方法及系统 |
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Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102586088B (zh) * | 2011-09-18 | 2013-04-24 | 淮北市三和诺生物工程有限责任公司 | 无菌超滤浓缩型酶制剂发酵液后提取生产线 |
CN102399685B (zh) * | 2011-09-18 | 2013-01-30 | 淮北市三和诺生物工程有限责任公司 | 成品残留液回收型酶制剂发酵液后提取生产线 |
CN102399686B (zh) * | 2011-09-18 | 2013-02-27 | 淮北市三和诺生物工程有限责任公司 | 酶制剂发酵液后提取生产线 |
CN102796205B (zh) * | 2012-08-24 | 2014-12-17 | 黄河三角洲京博化工研究院有限公司 | 一种高透明度小核菌葡聚糖的制备方法 |
CN102978257B (zh) * | 2012-12-20 | 2014-12-10 | 江苏久吾高科技股份有限公司 | 一种右旋糖酐的生产方法 |
CN103951762A (zh) * | 2014-05-23 | 2014-07-30 | 吉林农业大学 | 一种小核菌胞外多糖的提取分离方法 |
CN106337071A (zh) * | 2016-01-28 | 2017-01-18 | 通辽市黄河龙生物工程有限公司 | 小核菌硬葡聚糖工业量产发酵及提取工艺 |
CN108441429B (zh) * | 2018-03-22 | 2019-11-08 | 江南大学 | 一种小核菌及其发酵生产硬葡聚糖的方法 |
EP3549958A1 (en) * | 2018-04-04 | 2019-10-09 | Clariant International Ltd | Process for the purification of complex biocompositions |
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KR100594482B1 (ko) * | 1998-04-14 | 2006-07-03 | 디에스엠 아이피 어셋츠 비.브이. | 막 여과 |
CN1164506C (zh) * | 2001-04-09 | 2004-09-01 | 南京化工大学 | 陶瓷膜管生物反应分离系统 |
CN1245247C (zh) * | 2002-12-30 | 2006-03-15 | 北京迈胜普技术有限公司 | 利用陶瓷膜的生物发酵液分离方法 |
CN2622190Y (zh) * | 2003-05-13 | 2004-06-30 | 南京工业大学 | 无机膜生物反应装置 |
EP2550971B1 (en) * | 2004-09-30 | 2016-11-09 | Bayer HealthCare LLC | Devices and Methods for Integrated Continuous Manufacturing of Biological Molecules |
CN101392273B (zh) * | 2008-11-10 | 2013-02-06 | 南京工业大学 | 一种乳酸的清洁生产工艺 |
CN101544949A (zh) * | 2009-04-30 | 2009-09-30 | 南开大学 | 一种提高水溶性高聚物发酵产率的方法 |
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