RU2019138986A - Способы приготовления интенсивных подсластителей - Google Patents

Способы приготовления интенсивных подсластителей Download PDF

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RU2019138986A
RU2019138986A RU2019138986A RU2019138986A RU2019138986A RU 2019138986 A RU2019138986 A RU 2019138986A RU 2019138986 A RU2019138986 A RU 2019138986A RU 2019138986 A RU2019138986 A RU 2019138986A RU 2019138986 A RU2019138986 A RU 2019138986A
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mogroside
host cell
recombinant host
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compound
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RU2767792C2 (ru
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Эндрю П. ПЭТРОН
Крис Эдано НОРИГА
Рама Р. МАНАМ
Джастин КОЛКИТТ
Нейтан ФАБЕР
Хелдж ЗИЛЕР
Джастин СТИДЖ
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Фирмениш Инкорпорейтед
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
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    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99003—2-Acetolactate mutase (5.4.99.3)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

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Claims (18)

1. Способ получения Соединения 1, имеющего структуру:
Figure 00000001
(1),
где способ включает:
контакт могрозида IIIE с первым ферментом, способным катализировать получение соединения 1, из могрозида IIIE.
2. Способ по п. 1, в котором контакт могрозида IIIE с первым ферментом включает контакт могрозида IIIE с рекомбинантной клеткой-хозяином, которая включает первый ген, кодирующий первый фермент.
3. Способ по п. 2, в котором первый ген гетерологичен рекомбинантной клетке-хозяину.
4. Способ по любому из пп. 1-3, в котором первый фермент представляет собой одну или несколько из числа UDP-гликозилтрансфераз, цикломальтодекстрин-глюканотрансфераз (ЦГТаз), гликотрансфераз, декстрансукраз, целлюлаз, β-глюкозидаз, амилаз, трансглюкозидаз, пектиназ и декстраназ.
5. Способ по любому из пп. 1-4, включающий контакт могрозида IIA со вторым ферментом, способным катализировать образование могрозида IIIE из могрозида IIA.
6. Способ по любому из пп. 1-5, включающий контакт могрола с одним или несколькими ферментами, способными катализировать образование могрозида IIIE из могрола.
7. Способ по любому из пп. 1-6, включающий контакт соединения могрозида с одним или несколькими ферментами, способными катализировать образование могрозида IIIE из соединения могрозидов, с получением могрозида IIIE, где соединение могрозидов представляет собой один или несколько из числа могрозида IA1, могрозида IE1, могрозида IIA1, могрозида IIE, могрозида IIA, могрозида IIIA1, могрозида IIIA2, могрозида III, могрозида IV, могрозида IVA, могрозида V и сиаменозида.
8. Способ по п.7, в котором один или несколько ферментов, способных катализировать продуцирование могрозида IIIE из соединения могрозидов, включают одну или несколько из числа UDP-гликозилтрансфераз, ЦГТаз, гликотрансфераз, декстрансукраз, целлюлаз, β-глюкозидаз, амилаз, трансглюкозидаз, пектиназ и декстраназ.
9. Способ по любому из пп. 7 и 8, в котором соединение могрозида представляет собой моргрозид IIA или Могрозид IIE и где при контакте с одним или несколькими ферментами образуется один или несколько из числа могрозида IIIA, могрозида IVE и могрозида V.
10. Способ по любому из пп. 2-3, в котором способ включает контакт 11-гидрокси-24,25 эпоксикукурбитадиенола с рекомбинантной клеткой-хозяином, где рекомбинантная клетка-хозяин содержит третий ген, кодирующий эпоксидгидролазу, где 11-гидрокси-24,25 эпокси-кукурбитадиенол присутствует в и/или продуцируется рекомбинантной клеткой-хозяином, и где 11-гидрокси-кукурбитадиенол присутствует в и/или продуцируется рекомбинантной клеткой-хозяином.
11. Способ по п.10, в котором контакт приводит к продуцированию Могрола в рекомбинантной клетке-хозяине.
12. Способ по любому из пп. 2, 3, 10 и 11, в котором способ включает контакт кукурбитадиенола с рекомбинантной клеткой-хозяином, и где рекомбинантная клетка-хозяин содержит ген, кодирующий цитохром Р450, где контакт приводит к получению 11-гидроксикукурбитадиенола.
13. Способ по любому из пп. 2-3, и 10-12, в котором способ включает контакт одного или нескольких из числа 2,3-оксидосквалена, диоксидоксидвалена и диэпоксисквалена с рекомбинантной клеткой-хозяином, где рекомбинантная клетка-хозяин содержит седьмой ген, кодирующий полипептид, имеющий активность кукурбитадиенолсинтазы, в котором контакт приводит к получению кукурбитадиенола и/или 24,25-эпокси-кукурбитадиенола.
14. Способ по п.13, в котором один или несколько из 2,3-оксидосквалена, диоксидоксидвалена и диэпоксисквалена продуцируются и/или присутствуют в рекомбинантной клетке-хозяине.
15. Способ по любому из пп. 2, 3, и 10-14, в котором способ включает контакт фарнезилпирофосфата с рекомбинантной клеткой-хозяином, где рекомбинантная клетка-хозяин содержит девятый ген, кодирующий скваленсинтазу, где контакт приводит к получению сквалена.
RU2019138986A 2017-05-03 2018-05-02 Способы приготовления интенсивных подсластителей RU2767792C2 (ru)

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PCT/US2018/030627 WO2018204483A2 (en) 2017-05-03 2018-05-02 Methods for making high intensity sweeteners

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Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY190296A (en) 2015-10-29 2022-04-12 Firmenich Incorporated High intensity sweeteners
GB201805576D0 (en) * 2018-04-04 2018-05-16 Optibiotix Ltd Sweeteners and methods of production thereof
MX2021005220A (es) * 2018-11-07 2021-06-18 Firmenich Incorporated Metodos para hacer edulcorantes de alta intensidad.
JP2022512932A (ja) * 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
WO2020097588A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
US20220186266A1 (en) 2019-04-02 2022-06-16 Novozymes A/S Process For Producing A Fermentation Product
WO2020200916A1 (en) * 2019-04-04 2020-10-08 Firmenich Sa Mogroside compounds and uses thereof
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
CN115335514A (zh) * 2019-10-25 2022-11-11 银杏生物制品公司 罗汉果甙的生物合成
CN110726787B (zh) * 2019-11-11 2022-10-11 广西中烟工业有限责任公司 一种分析七种罗汉果皂苷的lc-ms/ms负离子模式检测方法
CN110669809B (zh) * 2019-11-12 2022-12-23 广西师范大学 一种酶法制备罗汉果苷iv和苷v的方法
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
US20230061835A1 (en) * 2020-03-05 2023-03-02 Firmenich Sa 11-oxo cucurbitanes and their use as flavor modifiers
EP4120823A1 (en) * 2020-03-17 2023-01-25 The Coca-Cola Company Novel mogroside production system and methods
US20230174993A1 (en) * 2020-05-13 2023-06-08 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN112626094A (zh) * 2021-01-15 2021-04-09 中国水产科学研究院黄海水产研究所 环糊精葡萄糖基转移酶的基因、氨基酸序列和应用
CN113429451B (zh) * 2021-05-31 2022-02-08 湖南华诚生物资源股份有限公司 一种罗汉果酸酰化衍生物及其制备方法
EP4333645A1 (en) * 2021-06-29 2024-03-13 Firmenich Incorporated Mogroside compounds and their comestible use
CN113481275A (zh) * 2021-07-23 2021-10-08 湖南华诚生物资源股份有限公司 一种酶催化半合成制备罗汉果赛门苷的方法
CN113584110A (zh) * 2021-08-11 2021-11-02 河北维达康生物科技有限公司 一种以罗汉果醇为底物生物合成罗汉果糖苷v的工程菌株的构建及其应用
CN114946910A (zh) * 2022-03-31 2022-08-30 广西朗盛食品科技有限公司 一种低糖红豆蛋黄酥及其制备方法
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6468576B1 (en) 2000-06-23 2002-10-22 Nestec S.A. Frozen slush liquid concentrate and method of making same
US7939059B2 (en) 2001-12-10 2011-05-10 California Institute Of Technology Method for the generation of antigen-specific lymphocytes
NZ545747A (en) 2003-08-06 2010-06-25 Senomyx Inc T1R hetero-oligomeric taste receptors, cell lines that express said receptors, and taste compounds
CA2597134C (en) 2005-02-04 2015-05-26 Senomyx, Inc. Compounds comprising linked heteroaryl moieties and their use as novel umami flavor modifiers, tastants and taste enhancers for comestible compositions
WO2006102435A2 (en) 2005-03-22 2006-09-28 Water Sensations, Inc. Flavoring composition concentrates
AR055329A1 (es) 2005-06-15 2007-08-15 Senomyx Inc Amidas bis-aromaticas y sus usos como modificadores de sabor dulce, saborizantes, y realzadores de sabor
WO2016130609A1 (en) 2015-02-10 2016-08-18 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
KR101387563B1 (ko) 2006-04-21 2014-04-25 세노믹스, 인코포레이티드 고효능의 조미용 향료를 포함하는 식품 조성물 및 이의 제조 방법
DK2024504T4 (da) 2006-05-26 2023-02-27 Amyris Inc Fremstilling af isoprenoider
WO2008130372A2 (en) 2006-09-28 2008-10-30 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
US7928111B2 (en) 2007-06-08 2011-04-19 Senomyx, Inc. Compounds including substituted thienopyrimidinone derivatives as ligands for modulating chemosensory receptors
EP2188300B1 (en) 2007-08-17 2013-01-30 Givaudan SA Novel sweetener iso-mogroside v
MX302107B (es) 2007-09-20 2012-08-08 Amyris Biotechnologies Inc Produccion de isoprenoides.
CN104193723B (zh) 2008-02-06 2019-05-31 西诺米克斯公司 增甜剂组合物及其制备方法
TWI429404B (zh) 2008-03-03 2014-03-11 Senomyx Inc 異山梨醇衍生物及彼等作為風味改良劑、促味劑及促味增強劑之用途
CN102170797B (zh) 2008-07-31 2014-02-19 西诺米克斯公司 包含甜味增强剂的组合物和它们的制备方法
WO2010014666A2 (en) 2008-07-31 2010-02-04 Senomyx, Inc. Processes and intermediates for making sweet taste enhancers
US8481286B2 (en) 2008-08-12 2013-07-09 Allylix, Inc. Method for production of isoprenoid compounds
BR122018010309B1 (pt) 2009-04-02 2020-11-10 Amyris, Inc. métodos de estabilização e de hidrogenação para olefinas micróbio-derivadas
US8357527B2 (en) 2009-06-01 2013-01-22 Amyris, Inc. Method for generating a genetically modified microbe
JP5978132B2 (ja) 2009-11-23 2016-08-24 ニューセリス インコーポレイテッド 酵母を用いるスクアレン生成のための方法および組成物
WO2011112892A1 (en) 2010-03-12 2011-09-15 Purecircle Usa Inc. High-purity steviol glycosides
JP5988506B2 (ja) 2010-04-02 2016-09-07 セノミックス インコーポレイテッド 甘味修飾物質
BR112012028932B8 (pt) 2010-05-21 2019-01-15 Amyris Inc método para preparação de isoesqualeno, métodos para preparação de esqualeno e de uma composição, composição, óleo de base lubrificante, e, formulação lubrificante
WO2012021837A2 (en) 2010-08-12 2012-02-16 Senomyx, Inc. Method of improving stability of sweet enhancer and composition containing stabilized sweet enhancer
BR112013011188B1 (pt) 2010-11-05 2022-01-04 Senomyx, Inc Método não-médico para modular canal potencial de receptor transiente de melastina 8 (trpm8), método não-médico para modular a sensação de resfriamento de uma composição, método não-médico para induzir uma sensação de resfriamento em um humano ou animal e composto
US8962698B2 (en) * 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
EP2670846B1 (en) 2011-02-02 2015-08-19 Amyris, Inc. Methods of developing terpene synthase variants
AU2012295255B2 (en) 2011-08-12 2016-04-21 Senomyx, Inc. Sweet flavor modifier
US8415136B1 (en) 2011-11-09 2013-04-09 Amyris, Inc. Production of acetyl-coenzyme a derived isoprenoids
KR101791597B1 (ko) * 2011-11-23 2017-10-30 에볼바 에스아 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료
PL3009010T3 (pl) 2011-12-19 2020-10-19 Purecircle Sdn Bhd Sposoby oczyszczania glikozydów stewiolowych
PE20191204A1 (es) 2012-08-06 2019-09-10 Firmenich Incorporated Modificador del sabor dulce
BR112015003329A2 (pt) 2012-08-17 2017-09-26 Evolva Sa produção aumentada de terpenos e terpenoides
US10633685B2 (en) * 2012-12-04 2020-04-28 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
EP2929043A1 (en) * 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
ES2717941T3 (es) 2013-02-19 2019-06-26 Senomyx Inc Compuestos útiles como moduladores de TRPM8
JO3155B1 (ar) 2013-02-19 2017-09-20 Senomyx Inc معدِّل نكهة حلوة
MX364748B (es) 2013-03-15 2019-05-06 Amyris Inc Uso de fosfoquetolasa y fosfotransacetilasa para la produccion de compuestos derivados de acetil-coenzima a.
GB201309077D0 (en) 2013-03-15 2013-07-03 Tate & Lyle Ingredients Improved sweetener
BR112016012682B1 (pt) * 2013-12-05 2021-12-07 Analyticon Discovery Gmbh Composto adoçante
US20180020709A1 (en) 2014-04-16 2018-01-25 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
CN103923152B (zh) 2014-05-08 2015-11-04 青岛润德生物科技有限公司 一种罗汉果甙v的提取方法
IL287789B2 (en) * 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
WO2016060276A1 (ja) * 2014-10-17 2016-04-21 サントリーホールディングス株式会社 モグロール配糖体化酵素およびそれをコードする遺伝子
CA2963901A1 (en) 2014-11-07 2016-05-12 Senomyx, Inc. Substituted 4-amino-5-(cyclohexyloxy)quinoline-3-carboxylic acids as sweet flavor modifiers
CN105039274A (zh) 2015-07-13 2015-11-11 中国农业科学院蔬菜花卉研究所 参与西瓜葫芦素e合成的基因簇及其应用
US20180250313A1 (en) 2015-09-08 2018-09-06 Viewpoint Therapeutics, Inc. Compounds and formulations for treating ophthalmic diseases
MY190296A (en) * 2015-10-29 2022-04-12 Firmenich Incorporated High intensity sweeteners
WO2017172766A1 (en) 2016-03-28 2017-10-05 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside or mogroside sweeteners with flavonids
EP3439488B1 (en) 2016-04-06 2020-12-02 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside or mogroside sweeteners
JP6845244B2 (ja) 2016-07-19 2021-03-17 サントリーホールディングス株式会社 モグロールまたはモグロール配糖体の生産方法
CA3061045A1 (en) * 2017-04-25 2018-11-01 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside and mogroside sweeteners with dihydrochalcones
US11248248B2 (en) 2017-06-15 2022-02-15 Evolva Sa Production of mogroside compounds in recombinant hosts

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