RU2017144220A - Среда для культуры клеток, не содержащая олигопептидов - Google Patents
Среда для культуры клеток, не содержащая олигопептидов Download PDFInfo
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- RU2017144220A RU2017144220A RU2017144220A RU2017144220A RU2017144220A RU 2017144220 A RU2017144220 A RU 2017144220A RU 2017144220 A RU2017144220 A RU 2017144220A RU 2017144220 A RU2017144220 A RU 2017144220A RU 2017144220 A RU2017144220 A RU 2017144220A
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- 102000015636 Oligopeptides Human genes 0.000 title claims 2
- 108010038807 Oligopeptides Proteins 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 21
- 210000004027 cell Anatomy 0.000 claims 13
- 239000002609 medium Substances 0.000 claims 10
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 5
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 5
- 108010054218 Factor VIII Proteins 0.000 claims 4
- 102000001690 Factor VIII Human genes 0.000 claims 4
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 4
- 239000005700 Putrescine Substances 0.000 claims 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims 2
- 108091005670 ADAMTS13 Proteins 0.000 claims 2
- 102000043853 ADAMTS13 Human genes 0.000 claims 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- 108010023321 Factor VII Proteins 0.000 claims 2
- 108090001126 Furin Proteins 0.000 claims 2
- 102000004961 Furin Human genes 0.000 claims 2
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 claims 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 2
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 claims 2
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 claims 2
- VHRGRCVQAFMJIZ-UHFFFAOYSA-N cadaverine Chemical compound NCCCCCN VHRGRCVQAFMJIZ-UHFFFAOYSA-N 0.000 claims 2
- 229940105778 coagulation factor viii Drugs 0.000 claims 2
- 229960003104 ornithine Drugs 0.000 claims 2
- 230000000737 periodic effect Effects 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims 2
- 229940063675 spermine Drugs 0.000 claims 2
- 108010047303 von Willebrand Factor Proteins 0.000 claims 2
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 claims 1
- QYPPJABKJHAVHS-UHFFFAOYSA-N Agmatine Natural products NCCCCNC(N)=N QYPPJABKJHAVHS-UHFFFAOYSA-N 0.000 claims 1
- 241000271566 Aves Species 0.000 claims 1
- 102100022641 Coagulation factor IX Human genes 0.000 claims 1
- 102100023804 Coagulation factor VII Human genes 0.000 claims 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims 1
- 108010076282 Factor IX Proteins 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- 235000019393 L-cystine Nutrition 0.000 claims 1
- 239000004158 L-cystine Substances 0.000 claims 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 1
- 229930182816 L-glutamine Natural products 0.000 claims 1
- 229930182821 L-proline Natural products 0.000 claims 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims 1
- QYPPJABKJHAVHS-UHFFFAOYSA-P agmatinium(2+) Chemical compound NC(=[NH2+])NCCCC[NH3+] QYPPJABKJHAVHS-UHFFFAOYSA-P 0.000 claims 1
- 229940024606 amino acid Drugs 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 239000003963 antioxidant agent Substances 0.000 claims 1
- 235000006708 antioxidants Nutrition 0.000 claims 1
- 229960005070 ascorbic acid Drugs 0.000 claims 1
- 235000010323 ascorbic acid Nutrition 0.000 claims 1
- 239000011668 ascorbic acid Substances 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000023555 blood coagulation Effects 0.000 claims 1
- 239000012928 buffer substance Substances 0.000 claims 1
- 239000006172 buffering agent Substances 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 229940105774 coagulation factor ix Drugs 0.000 claims 1
- 229940105772 coagulation factor vii Drugs 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 claims 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims 1
- 229940031098 ethanolamine Drugs 0.000 claims 1
- 229960002743 glutamine Drugs 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims 1
- 230000010412 perfusion Effects 0.000 claims 1
- 229960002429 proline Drugs 0.000 claims 1
- 239000003531 protein hydrolysate Substances 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011781 sodium selenite Substances 0.000 claims 1
- 229960001471 sodium selenite Drugs 0.000 claims 1
- 235000015921 sodium selenite Nutrition 0.000 claims 1
- 229940063673 spermidine Drugs 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 229960004799 tryptophan Drugs 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 102100036537 von Willebrand factor Human genes 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Claims (20)
1. Способ увеличения экспрессии рекомбинантного белка в культуре клеток, включающий культивирование клеток, которые экспрессируют рекомбинантный белок, в безбелковой среде, которая не содержит олигопептиды, причем среда содержит аминокислоты, витамины, органические и неорганические соли и источников углеводов и дополнена по меньшей мере 2 мг/л путресцина.2HCl.
2. Способ по п.1, где среда дополнена L-глутамином, аскорбиновой кислотой, этаноламином, селенитом натрия и неионным поверхностно-активным веществом.
3. Способ по п.1 или 2, где среда дополнена Fe(II) или Cu(II) в количестве, большем, чем количество в основной DMEM/HAM's F12 (1:1).
4. Способ по любому из предшествующих пунктов, где среда дополнена L-аспарагином, L-цистеином, L-цистином, L-пролином и L-триптофаном.
5. Способ по любому из предшествующих пунктов, где среда содержит путресцин в концентрации от примерно 1,0 мг/л до примерно 20 мг/л.
6. Способ по любому из предшествующих пунктов, где среда дополнительно содержит один или более из группы, состоящей из кадаверина, спермидина, спермина, агматина и орнитина.
7. Способ по любому из предшествующих пунктов, где среда дополнительно содержит орнитин, спермин или их комбинацию.
8. Способ по любому из предшествующих пунктов, где среда содержит буферное вещество.
9. Способ по п.8, где буферное вещество выбрано из одного или нескольких из бикарбонатов натрия, антиоксидантов, стабилизаторов или ингибиторов протеазы.
10. Способ по любому из предшествующих пунктов, где рекомбинантный белок выбран из группы, состоящей из фактора свертывания крови VII, фактора свертывания крови VIII, фактора свертывания крови IX, vWF, ADAMTS13 и фурина.
11. Способ по п.10, где рекомбинантный белок представляет собой фактор свертывания крови VIII.
12. Способ по п.10, где рекомбинантный белок представляет собой vWF.
13. Способ по любому из предшествующих пунктов, где клетки выбраны из группы, состоящей из клеток млекопитающих, клеток насекомых, клеток птиц, бактериальных клеток и дрожжевых клеток.
14. Способ по любому из предшествующих пунктов, где клетки представляют собой клетки СНО, клетки 293 или клетки BHK.
15. Способ по любому из предшествующих пунктов, где комбинация клетка/белок выбрана из группы, состоящей из клетки СНО/фактор свертывания крови VIII, клетки СНО/фактор свертывания крови VII, клетки CHO/ADAMTS13, клетки СНО/фурин и клетки 293/фактор свертывания крови IX.
16. Способ по п.15, где комбинация клетка/белок представляет собой клетки CHO/фактор свертывания крови VIII.
17. Способ по любому из предшествующих пунктов, где путресцин получен синтетическим путем.
18. Способ по любому из предшествующих пунктов, где путресцин происходит от источника, отличного от гидролизата белка.
19. Способ по любому из предшествующих пунктов, где среда химически определена.
20. Способ по любому из предшествующих пунктов, где клетки культивируют способом, выбранным из группы, состоящей из периодического культивирования, периодического культивирования с подпиткой, перфузионного культивирования и хемостатического культивирования.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75641906P | 2006-01-04 | 2006-01-04 | |
| US60/756,419 | 2006-01-04 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013112019A Division RU2642269C9 (ru) | 2006-01-04 | 2013-03-18 | Среда для культуры клеток, не содержащая олигопептидов |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017144220A true RU2017144220A (ru) | 2019-06-18 |
| RU2017144220A3 RU2017144220A3 (ru) | 2021-03-01 |
| RU2758802C2 RU2758802C2 (ru) | 2021-11-02 |
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008131953/10A RU2486236C2 (ru) | 2006-01-04 | 2007-01-03 | Способ экспрессии белка |
| RU2013112019A RU2642269C9 (ru) | 2006-01-04 | 2013-03-18 | Среда для культуры клеток, не содержащая олигопептидов |
| RU2017144220A RU2758802C2 (ru) | 2006-01-04 | 2017-12-18 | Среда для культуры клеток, не содержащая олигопептидов |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008131953/10A RU2486236C2 (ru) | 2006-01-04 | 2007-01-03 | Способ экспрессии белка |
| RU2013112019A RU2642269C9 (ru) | 2006-01-04 | 2013-03-18 | Среда для культуры клеток, не содержащая олигопептидов |
Country Status (18)
| Country | Link |
|---|---|
| US (4) | US20070212770A1 (ru) |
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| PL3121266T3 (pl) | 2006-01-04 | 2020-07-13 | Baxalta Incorporated | Wolne od oligopeptydów pożywki do hodowli komórkowej |
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