BR0212780A - Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas - Google Patents

Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas

Info

Publication number
BR0212780A
BR0212780A BR0212780-6A BR0212780A BR0212780A BR 0212780 A BR0212780 A BR 0212780A BR 0212780 A BR0212780 A BR 0212780A BR 0212780 A BR0212780 A BR 0212780A
Authority
BR
Brazil
Prior art keywords
polypeptides
eukaryotic cells
producing
methods
harvesting
Prior art date
Application number
BR0212780-6A
Other languages
English (en)
Inventor
Ida Molgaard Knudsen
Original Assignee
Novo Nordisk Healthcare Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/DK2001/000634 external-priority patent/WO2002029084A2/en
Application filed by Novo Nordisk Healthcare Ag filed Critical Novo Nordisk Healthcare Ag
Publication of BR0212780A publication Critical patent/BR0212780A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"MéTODOS PARA PRODUZIR POLIPEPTìDEOS EM CéLULAS EUCARIóTICAS, PARA CULTIVAR CéLULAS EUCARIóTICAS E PARA COLHER POLIPEPTìDEOS PRODUZIDOS POR CéLULAS EUCARIóTICAS". A invenção proporciona um método para produzir polipeptídeos em cultura de células eucarióticas em microveículos, sendo que o método compreende as etapas de (i) cultivar células que expressam referido polipeptídeo sobre microveículos em condições e a uma temperatura de ponto de ajuste apropriadas para expressão do referido polipeptídeo; (ii) resfriar a cultura a uma temperatura predeterminada abaixo de referido ponto de ajuste; (iii) sedimentar os microveículos; e (iv) colher todo ou parte do meio de cultura.
BR0212780-6A 2001-10-02 2002-09-20 Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas BR0212780A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/DK2001/000634 WO2002029084A2 (en) 2000-10-02 2001-10-02 Industrial-scale serum-free production of recombinant factor vii in mammalian cells
PCT/DK2001/000632 WO2002029083A2 (en) 2000-10-02 2001-10-02 Industrial-scale serum-free production of recombinant proteins in mammalian cells
DKPA200200460 2002-03-26
PCT/DK2002/000612 WO2003029442A1 (en) 2001-10-02 2002-09-20 Method for production of recombinant proteins in eukaryote cells

Publications (1)

Publication Number Publication Date
BR0212780A true BR0212780A (pt) 2004-10-13

Family

ID=33104004

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0212780-6A BR0212780A (pt) 2001-10-02 2002-09-20 Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas

Country Status (13)

Country Link
US (3) US20030096366A1 (pt)
EP (1) EP1434857B1 (pt)
JP (1) JP4351049B2 (pt)
KR (1) KR20040039472A (pt)
CN (1) CN1309825C (pt)
AT (1) ATE368730T1 (pt)
BR (1) BR0212780A (pt)
CA (1) CA2460206C (pt)
DE (1) DE60221553T2 (pt)
ES (1) ES2291534T3 (pt)
HU (1) HUP0402158A2 (pt)
PL (1) PL368119A1 (pt)
WO (1) WO2003029442A1 (pt)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
WO2005035748A1 (en) 2003-10-10 2005-04-21 Novo Nordisk Health Care Ag Method for large-scale production of a polypeptide in eukaryote cells and a culture vessel suitable therefor
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
ES2395544T3 (es) 2004-12-23 2013-02-13 Novo Nordisk Health Care Ag Reducción del contenido de contaminantes proteínicos en composiciones que comprenden una proteína dependiente de la vitamina K de interés
EP1851305B1 (en) * 2005-02-11 2012-01-18 Novo Nordisk Health Care AG Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
US8030034B2 (en) * 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix
PL2522717T3 (pl) 2006-01-04 2014-08-29 Baxalta Inc Pożywki do hodowli komórkowych wolne od oligopeptydów
BRPI0716762A2 (pt) 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
PL2147096T3 (pl) * 2007-04-13 2015-08-31 Catalyst Biosciences Inc Zmodyfikowane polipeptydy czynnika VII i ich zastosowania
KR20190140090A (ko) 2007-07-09 2019-12-18 제넨테크, 인크. 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지
EP3255152A1 (en) * 2007-12-27 2017-12-13 Baxalta GmbH Cell culture processes
TWI538916B (zh) 2008-04-11 2016-06-21 介控生化科技公司 經修飾的因子vii多肽和其用途
PL2307538T3 (pl) * 2008-07-15 2016-03-31 Crucell Holland Bv Skalowalny proces do hodowania komórek PER.C6 i wytwarzania z nich produktów
CA2738499A1 (en) 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
EP2921501A1 (en) 2008-10-20 2015-09-23 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
HUE026210T2 (en) * 2008-11-12 2016-05-30 Baxalta Inc Procedure for serum-free, insulin-free VII. factor
CA2769003C (en) * 2009-07-23 2018-10-16 Xcellerex, Inc. Drain down and re-feed of microcarrier bioreactor
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
CN102858953B (zh) 2010-04-26 2015-09-09 诺瓦提斯公司 改进的细胞培养基
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
AU2014218723B2 (en) * 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
CA2901940A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US10004767B2 (en) 2013-09-19 2018-06-26 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for producing hepatocyte-like cells
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
ES2803773T3 (es) 2015-12-02 2021-01-29 CSL Behring Lengnau AG Medios mejorados para la expresión de proteínas recombinantes dependientes de vitamina k
KR102134571B1 (ko) * 2018-06-15 2020-07-16 주식회사 알테오젠 혈액응고인자 VII/VIIa 융합단백질의 제조 방법
EP3833381B1 (en) 2019-08-15 2022-08-03 Catalyst Biosciences, Inc. Modified factor vii polypeptides for subcutaneous administration

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4189534A (en) * 1976-11-11 1980-02-19 Massachusetts Institute Of Technology Cell culture microcarriers
US4357422A (en) * 1980-08-14 1982-11-02 Massachusetts Institute Of Technology Method of enhancing interferon production
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
US4357442A (en) * 1981-12-07 1982-11-02 The B. F. Goodrich Company Stable latexes of carboxyl containing polymers
US4664912A (en) * 1984-10-01 1987-05-12 Wiktor Tadeusz J Process for the large scale production of rabies vaccine
US4978616A (en) * 1985-02-28 1990-12-18 Verax Corporation Fluidized cell cultivation process
IL75994A0 (en) 1985-08-01 1985-12-31 Israel Inst Biolog Res Process for the production of a substance for monitoring cancer patients
SE464816B (sv) * 1985-10-15 1991-06-17 Nilsson Kjell Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning
GB2184759B (en) * 1985-12-28 1990-07-18 Shimizu Construction Co Ltd Concrete-filled tubular steel piece, concrete-filled steel tube column and method of constructing same.
US5576194A (en) * 1986-07-11 1996-11-19 Bayer Corporation Recombinant protein production
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
US5661008A (en) * 1991-03-15 1997-08-26 Kabi Pharmacia Ab Recombinant human factor VIII derivatives
IL104385A (en) * 1992-01-17 1995-12-31 Applied Research Systems Method and device for growing biomass particles
DK53792D0 (da) 1992-04-24 1992-04-24 Novo Nordisk As Fremgangsmaade til fremstilling af proteiner
WO1993022448A1 (en) 1992-05-01 1993-11-11 Teijin Limited Fed batch culture method for protein secreting cells
US5733776A (en) * 1993-11-09 1998-03-31 Genzyme Corporation Continuous settling apparatus
US5510328A (en) * 1994-04-28 1996-04-23 La Jolla Cancer Research Foundation Compositions that inhibit wound contraction and methods of using same
US5645197A (en) * 1996-07-01 1997-07-08 Chen; Guan-Zhon Adjustable volume dosing dispenser for fish food
WO1998004680A1 (en) 1996-07-26 1998-02-05 University Of Manitoba Serum-free medium for growth of anchorage-dependant mammalian cells
PT973544E (pt) * 1997-04-08 2001-12-28 Baxter Ag Preparacao de complexo de protrombina imunotolerante
US6475725B1 (en) * 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT407255B (de) * 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
SE514189C2 (sv) * 1998-04-29 2001-01-22 Emba Machinery Ab Slitsenhet för kartongämnestillverkning
ATE408020T1 (de) 1998-11-06 2008-09-15 Novo Nordisk Healthcare Ag Verfahren zur herstellung von faktor vii
JP2003506077A (ja) 1999-08-05 2003-02-18 バクスター アクチェンゲゼルシャフト 組換え安定細胞クローン、その産生およびその使用
UA74557C2 (en) * 1999-09-03 2006-01-16 Applied Research Systems A method for producing a heterologous secreted protein from chinese hamster ovaries cells grown on microcarriers
DE60137950D1 (de) 2000-10-02 2009-04-23 Novo Nordisk Healthcare Ag Verfahren zur herstellung vitamin-k-abhängiger proteine

Also Published As

Publication number Publication date
PL368119A1 (en) 2005-03-21
EP1434857B1 (en) 2007-08-01
US20060194289A1 (en) 2006-08-31
CA2460206A1 (en) 2003-04-10
JP2005518190A (ja) 2005-06-23
US7947471B2 (en) 2011-05-24
JP4351049B2 (ja) 2009-10-28
CN1309825C (zh) 2007-04-11
US20080064068A1 (en) 2008-03-13
ES2291534T3 (es) 2008-03-01
DE60221553T2 (de) 2008-04-10
HUP0402158A2 (hu) 2005-01-28
WO2003029442A1 (en) 2003-04-10
EP1434857A1 (en) 2004-07-07
KR20040039472A (ko) 2004-05-10
US20030096366A1 (en) 2003-05-22
CN1564863A (zh) 2005-01-12
DE60221553D1 (de) 2007-09-13
ATE368730T1 (de) 2007-08-15
CA2460206C (en) 2012-01-24

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