RU2015106549A - Высокопроизводительная сборка фрагментов днк - Google Patents
Высокопроизводительная сборка фрагментов днк Download PDFInfo
- Publication number
- RU2015106549A RU2015106549A RU2015106549A RU2015106549A RU2015106549A RU 2015106549 A RU2015106549 A RU 2015106549A RU 2015106549 A RU2015106549 A RU 2015106549A RU 2015106549 A RU2015106549 A RU 2015106549A RU 2015106549 A RU2015106549 A RU 2015106549A
- Authority
- RU
- Russia
- Prior art keywords
- dna
- dna molecule
- sequence
- restriction enzyme
- type
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title claims abstract 22
- 108020004414 DNA Proteins 0.000 claims abstract 51
- 102000053602 DNA Human genes 0.000 claims abstract 29
- 238000000034 method Methods 0.000 claims abstract 20
- 238000003776 cleavage reaction Methods 0.000 claims abstract 14
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract 14
- 230000007017 scission Effects 0.000 claims abstract 14
- 108010091086 Recombinases Proteins 0.000 claims abstract 7
- 102000018120 Recombinases Human genes 0.000 claims abstract 7
- 108090000790 Enzymes Proteins 0.000 claims abstract 3
- 102000004190 Enzymes Human genes 0.000 claims abstract 3
- 230000006820 DNA synthesis Effects 0.000 claims abstract 2
- 238000012408 PCR amplification Methods 0.000 claims abstract 2
- 239000003550 marker Substances 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 3
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 108700005090 Lethal Genes Proteins 0.000 claims 2
- 108010010574 Tn3 resolvase Proteins 0.000 claims 2
- 229960000723 ampicillin Drugs 0.000 claims 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 229930027917 kanamycin Natural products 0.000 claims 2
- 229960000318 kanamycin Drugs 0.000 claims 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims 2
- 229930182823 kanamycin A Natural products 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 230000004544 DNA amplification Effects 0.000 claims 1
- 101150102092 ccdB gene Proteins 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261675929P | 2012-07-26 | 2012-07-26 | |
| US61/675,929 | 2012-07-26 | ||
| PCT/US2013/051641 WO2014018512A1 (en) | 2012-07-26 | 2013-07-23 | High-throughput dna fragment assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015106549A true RU2015106549A (ru) | 2016-09-20 |
Family
ID=48914467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015106549A RU2015106549A (ru) | 2012-07-26 | 2013-07-23 | Высокопроизводительная сборка фрагментов днк |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9752154B2 (enExample) |
| EP (1) | EP2877581B1 (enExample) |
| JP (1) | JP2015525567A (enExample) |
| KR (1) | KR20150032306A (enExample) |
| CN (1) | CN104619847A (enExample) |
| AU (1) | AU2013293123B2 (enExample) |
| BR (1) | BR112015001316A2 (enExample) |
| CA (1) | CA2882143A1 (enExample) |
| IL (1) | IL236814A0 (enExample) |
| IN (1) | IN2014DN11250A (enExample) |
| RU (1) | RU2015106549A (enExample) |
| WO (1) | WO2014018512A1 (enExample) |
| ZA (1) | ZA201409509B (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107002150B (zh) * | 2015-01-09 | 2020-09-01 | 深圳华大基因研究院 | 一种dna合成产物的高通量检测方法 |
| CN106318961B (zh) * | 2015-06-30 | 2019-07-09 | 未名生物农业集团有限公司 | 一种多dna片段载体的构建方法及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| EP0290455A4 (en) | 1986-01-08 | 1989-01-18 | Rhone Poulenc Agrochimie | HALOARYLNITRILE DEGREASING GENE, THE USE THEREOF AND THE CELLS THAT CONTAIN IT. |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ATE57390T1 (de) | 1986-03-11 | 1990-10-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| JPH05506578A (ja) | 1990-04-18 | 1993-09-30 | プラント・ジエネテイツク・システムズ・エヌ・ベー | 変性ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現 |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| TW261517B (enExample) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8877916B2 (en) * | 2000-04-26 | 2014-11-04 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| CA2430947A1 (en) * | 2000-12-08 | 2002-06-13 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| CN1863914B (zh) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | 新的草甘膦-n-乙酰转移酶(gat)基因 |
| JP2007526215A (ja) * | 2003-05-05 | 2007-09-13 | ダウ アグロサイエンス リミテッド ライアビリティー カンパニー | トランスジェニック植物細胞に由来する安定な免疫予防的および治療的組成物ならびにその産生方法 |
| CA2897475C (en) | 2004-04-30 | 2018-07-10 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| EP3241430B1 (en) | 2005-10-28 | 2020-08-26 | Dow AgroSciences LLC | Novel herbicide resistance genes |
-
2013
- 2013-07-23 CA CA2882143A patent/CA2882143A1/en not_active Abandoned
- 2013-07-23 JP JP2015524385A patent/JP2015525567A/ja active Pending
- 2013-07-23 AU AU2013293123A patent/AU2013293123B2/en active Active
- 2013-07-23 IN IN11250DEN2014 patent/IN2014DN11250A/en unknown
- 2013-07-23 US US14/416,799 patent/US9752154B2/en active Active
- 2013-07-23 RU RU2015106549A patent/RU2015106549A/ru not_active Application Discontinuation
- 2013-07-23 WO PCT/US2013/051641 patent/WO2014018512A1/en not_active Ceased
- 2013-07-23 CN CN201380039369.7A patent/CN104619847A/zh active Pending
- 2013-07-23 KR KR1020157001623A patent/KR20150032306A/ko not_active Withdrawn
- 2013-07-23 BR BR112015001316A patent/BR112015001316A2/pt not_active IP Right Cessation
- 2013-07-23 EP EP13744922.9A patent/EP2877581B1/en active Active
-
2014
- 2014-12-23 ZA ZA2014/09509A patent/ZA201409509B/en unknown
-
2015
- 2015-01-20 IL IL236814A patent/IL236814A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ZA201409509B (en) | 2016-08-31 |
| BR112015001316A2 (pt) | 2017-08-01 |
| JP2015525567A (ja) | 2015-09-07 |
| EP2877581A1 (en) | 2015-06-03 |
| CA2882143A1 (en) | 2014-01-30 |
| US20150176015A1 (en) | 2015-06-25 |
| US9752154B2 (en) | 2017-09-05 |
| AU2013293123B2 (en) | 2018-04-05 |
| EP2877581B1 (en) | 2017-11-29 |
| KR20150032306A (ko) | 2015-03-25 |
| IL236814A0 (en) | 2015-03-31 |
| IN2014DN11250A (enExample) | 2015-10-09 |
| AU2013293123A1 (en) | 2015-01-22 |
| WO2014018512A1 (en) | 2014-01-30 |
| CN104619847A (zh) | 2015-05-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20171018 |