JP2015525567A5 - - Google Patents
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- Publication number
- JP2015525567A5 JP2015525567A5 JP2015524385A JP2015524385A JP2015525567A5 JP 2015525567 A5 JP2015525567 A5 JP 2015525567A5 JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015525567 A5 JP2015525567 A5 JP 2015525567A5
- Authority
- JP
- Japan
- Prior art keywords
- dna molecule
- dna
- sequence
- restriction enzyme
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004414 DNA Proteins 0.000 claims description 57
- 102000053602 DNA Human genes 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 28
- 239000012634 fragment Substances 0.000 claims description 24
- 108091008146 restriction endonucleases Proteins 0.000 claims description 21
- 239000013598 vector Substances 0.000 claims description 16
- 108010091086 Recombinases Proteins 0.000 claims description 8
- 102000018120 Recombinases Human genes 0.000 claims description 8
- 239000003550 marker Substances 0.000 claims description 8
- 230000029087 digestion Effects 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108700005090 Lethal Genes Proteins 0.000 claims description 4
- 108010010677 Phosphodiesterase I Proteins 0.000 claims description 4
- 229960000723 ampicillin Drugs 0.000 claims description 4
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 229930027917 kanamycin Natural products 0.000 claims description 4
- 229960000318 kanamycin Drugs 0.000 claims description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims description 4
- 229930182823 kanamycin A Natural products 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 230000004544 DNA amplification Effects 0.000 claims description 2
- 230000006820 DNA synthesis Effects 0.000 claims description 2
- 238000012408 PCR amplification Methods 0.000 claims description 2
- 101150102092 ccdB gene Proteins 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 claims description 2
- 108020005345 3' Untranslated Regions Proteins 0.000 description 2
- 108010010574 Tn3 resolvase Proteins 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261675929P | 2012-07-26 | 2012-07-26 | |
| US61/675,929 | 2012-07-26 | ||
| PCT/US2013/051641 WO2014018512A1 (en) | 2012-07-26 | 2013-07-23 | High-throughput dna fragment assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015525567A JP2015525567A (ja) | 2015-09-07 |
| JP2015525567A5 true JP2015525567A5 (enExample) | 2016-06-23 |
Family
ID=48914467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015524385A Pending JP2015525567A (ja) | 2012-07-26 | 2013-07-23 | ハイスループットｄｎａフラグメントアセンブリ |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9752154B2 (enExample) |
| EP (1) | EP2877581B1 (enExample) |
| JP (1) | JP2015525567A (enExample) |
| KR (1) | KR20150032306A (enExample) |
| CN (1) | CN104619847A (enExample) |
| AU (1) | AU2013293123B2 (enExample) |
| BR (1) | BR112015001316A2 (enExample) |
| CA (1) | CA2882143A1 (enExample) |
| IL (1) | IL236814A0 (enExample) |
| IN (1) | IN2014DN11250A (enExample) |
| RU (1) | RU2015106549A (enExample) |
| WO (1) | WO2014018512A1 (enExample) |
| ZA (1) | ZA201409509B (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107002150B (zh) * | 2015-01-09 | 2020-09-01 | 深圳华大基因研究院 | 一种dna合成产物的高通量检测方法 |
| CN106318961B (zh) * | 2015-06-30 | 2019-07-09 | 未名生物农业集团有限公司 | 一种多dna片段载体的构建方法及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| EP0290455A4 (en) | 1986-01-08 | 1989-01-18 | Rhone Poulenc Agrochimie | HALOARYLNITRILE DEGREASING GENE, THE USE THEREOF AND THE CELLS THAT CONTAIN IT. |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ATE57390T1 (de) | 1986-03-11 | 1990-10-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| JPH05506578A (ja) | 1990-04-18 | 1993-09-30 | プラント・ジエネテイツク・システムズ・エヌ・ベー | 変性ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現 |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| TW261517B (enExample) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8877916B2 (en) * | 2000-04-26 | 2014-11-04 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| CA2430947A1 (en) * | 2000-12-08 | 2002-06-13 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| CN1863914B (zh) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | 新的草甘膦-n-乙酰转移酶(gat)基因 |
| JP2007526215A (ja) * | 2003-05-05 | 2007-09-13 | ダウ アグロサイエンス リミテッド ライアビリティー カンパニー | トランスジェニック植物細胞に由来する安定な免疫予防的および治療的組成物ならびにその産生方法 |
| CA2897475C (en) | 2004-04-30 | 2018-07-10 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| EP3241430B1 (en) | 2005-10-28 | 2020-08-26 | Dow AgroSciences LLC | Novel herbicide resistance genes |
-
2013
- 2013-07-23 CA CA2882143A patent/CA2882143A1/en not_active Abandoned
- 2013-07-23 JP JP2015524385A patent/JP2015525567A/ja active Pending
- 2013-07-23 AU AU2013293123A patent/AU2013293123B2/en active Active
- 2013-07-23 IN IN11250DEN2014 patent/IN2014DN11250A/en unknown
- 2013-07-23 US US14/416,799 patent/US9752154B2/en active Active
- 2013-07-23 RU RU2015106549A patent/RU2015106549A/ru not_active Application Discontinuation
- 2013-07-23 WO PCT/US2013/051641 patent/WO2014018512A1/en not_active Ceased
- 2013-07-23 CN CN201380039369.7A patent/CN104619847A/zh active Pending
- 2013-07-23 KR KR1020157001623A patent/KR20150032306A/ko not_active Withdrawn
- 2013-07-23 BR BR112015001316A patent/BR112015001316A2/pt not_active IP Right Cessation
- 2013-07-23 EP EP13744922.9A patent/EP2877581B1/en active Active
-
2014
- 2014-12-23 ZA ZA2014/09509A patent/ZA201409509B/en unknown
-
2015
- 2015-01-20 IL IL236814A patent/IL236814A0/en unknown
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