BR112015001316A2 - montagem de fragmento de dna de alta produção - Google Patents

montagem de fragmento de dna de alta produção

Info

Publication number
BR112015001316A2
BR112015001316A2 BR112015001316A BR112015001316A BR112015001316A2 BR 112015001316 A2 BR112015001316 A2 BR 112015001316A2 BR 112015001316 A BR112015001316 A BR 112015001316A BR 112015001316 A BR112015001316 A BR 112015001316A BR 112015001316 A2 BR112015001316 A2 BR 112015001316A2
Authority
BR
Brazil
Prior art keywords
dna fragment
high production
methods
fragment assembly
production dna
Prior art date
Application number
BR112015001316A
Other languages
English (en)
Inventor
Gupta Manju
Kumar Sandeep
L Evans Steven
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112015001316A2 publication Critical patent/BR112015001316A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

resumo patente de invenção: "montagem de fragmento de dna de alta produção". esta invenção está relacionada aos métodos e sistemas para montagem de vetor para plantas transgênicas. um processo modular uniforme é usado para reduzir o tempo de ciclo e os métodos e sistemas fornecidos aqui aumentar a produtividade de clonagem usando placas de múltiplos poços, por exemplo, placas de 96 poços. em algumas modalidades, os métodos e sistemas fornecidos aqui eliminam ou reduzem a necessidade de confirmação de sequenciamento porque nenhuma pcr é envolvida no processo de montagem de vetor.
BR112015001316A 2012-07-26 2013-07-23 montagem de fragmento de dna de alta produção BR112015001316A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261675929P 2012-07-26 2012-07-26
PCT/US2013/051641 WO2014018512A1 (en) 2012-07-26 2013-07-23 High-throughput dna fragment assembly

Publications (1)

Publication Number Publication Date
BR112015001316A2 true BR112015001316A2 (pt) 2017-08-01

Family

ID=48914467

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015001316A BR112015001316A2 (pt) 2012-07-26 2013-07-23 montagem de fragmento de dna de alta produção

Country Status (13)

Country Link
US (1) US9752154B2 (pt)
EP (1) EP2877581B1 (pt)
JP (1) JP2015525567A (pt)
KR (1) KR20150032306A (pt)
CN (1) CN104619847A (pt)
AU (1) AU2013293123B2 (pt)
BR (1) BR112015001316A2 (pt)
CA (1) CA2882143A1 (pt)
IL (1) IL236814A0 (pt)
IN (1) IN2014DN11250A (pt)
RU (1) RU2015106549A (pt)
WO (1) WO2014018512A1 (pt)
ZA (1) ZA201409509B (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002150B (zh) * 2015-01-09 2020-09-01 深圳华大基因研究院 一种dna合成产物的高通量检测方法
CN106318961B (zh) * 2015-06-30 2019-07-09 未名生物农业集团有限公司 一种多dna片段载体的构建方法及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
JPH0795952B2 (ja) 1986-01-08 1995-10-18 カルジ−ン・インコ−ポレイテツド ハロアリ−ルニトリル分解遺伝子、その使用および該遺伝子含有細胞
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
DE3765449D1 (de) 1986-03-11 1990-11-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
JPH05506578A (ja) 1990-04-18 1993-09-30 プラント・ジエネテイツク・システムズ・エヌ・ベー 変性ｂａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US8877916B2 (en) * 2000-04-26 2014-11-04 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
EP2105496B1 (en) * 2000-12-08 2013-02-20 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP2535414B1 (en) 2003-04-29 2017-12-13 Pioneer Hi-Bred International Inc. Novel glyphosate-n-acetyltransferase (gat) genes
EP1635772A4 (en) * 2003-05-05 2008-02-13 Dow Agrosciences Llc STERILE IMMUNOPROPHYLACTIC AND THERAPEUTIC COMPOSITIONS DERIVED FROM TRANSGENIC VEGETABLE CELLS AND METHODS OF PRODUCTION THEREOF
NZ550602A (en) 2004-04-30 2009-11-27 Dow Agrosciences Llc Plants comprising herbicide resistance gene encoding aryloxyalkanoate dioxygenase
EP1947926B1 (en) 2005-10-28 2014-11-26 Dow AgroSciences LLC Novel herbicide resistance genes

Also Published As

Publication number Publication date
KR20150032306A (ko) 2015-03-25
RU2015106549A (ru) 2016-09-20
EP2877581B1 (en) 2017-11-29
EP2877581A1 (en) 2015-06-03
JP2015525567A (ja) 2015-09-07
CN104619847A (zh) 2015-05-13
US9752154B2 (en) 2017-09-05
US20150176015A1 (en) 2015-06-25
IL236814A0 (en) 2015-03-31
AU2013293123B2 (en) 2018-04-05
WO2014018512A1 (en) 2014-01-30
IN2014DN11250A (pt) 2015-10-09
AU2013293123A1 (en) 2015-01-22
ZA201409509B (en) 2016-08-31
CA2882143A1 (en) 2014-01-30

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2524 DE 21-05-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.