IN2014DN11250A - - Google Patents

Info

Publication number
IN2014DN11250A
IN2014DN11250A IN11250DEN2014A IN2014DN11250A IN 2014DN11250 A IN2014DN11250 A IN 2014DN11250A IN 11250DEN2014 A IN11250DEN2014 A IN 11250DEN2014A IN 2014DN11250 A IN2014DN11250 A IN 2014DN11250A
Authority
IN
India
Prior art keywords
methods
well plates
systems
reduce
systems provided
Prior art date
Application number
Inventor
Sandeep Kumar
Steven L Evans
Manju Gupta
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of IN2014DN11250A publication Critical patent/IN2014DN11250A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple well plates for example 96 well plates. In some embodiments the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.
IN11250DEN2014 2012-07-26 2013-07-23 IN2014DN11250A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261675929P 2012-07-26 2012-07-26
PCT/US2013/051641 WO2014018512A1 (en) 2012-07-26 2013-07-23 High-throughput dna fragment assembly

Publications (1)

Publication Number Publication Date
IN2014DN11250A true IN2014DN11250A (en) 2015-10-09

Family

ID=48914467

Family Applications (1)

Application Number Title Priority Date Filing Date
IN11250DEN2014 IN2014DN11250A (en) 2012-07-26 2013-07-23

Country Status (13)

Country Link
US (1) US9752154B2 (en)
EP (1) EP2877581B1 (en)
JP (1) JP2015525567A (en)
KR (1) KR20150032306A (en)
CN (1) CN104619847A (en)
AU (1) AU2013293123B2 (en)
BR (1) BR112015001316A2 (en)
CA (1) CA2882143A1 (en)
IL (1) IL236814A0 (en)
IN (1) IN2014DN11250A (en)
RU (1) RU2015106549A (en)
WO (1) WO2014018512A1 (en)
ZA (1) ZA201409509B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002150B (en) * 2015-01-09 2020-09-01 深圳华大基因研究院 High-throughput detection method for DNA synthesis product
CN106318961B (en) * 2015-06-30 2019-07-09 未名生物农业集团有限公司 A kind of construction method of more DNA fragmentation carriers and its application

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
WO1987004181A1 (en) 1986-01-08 1987-07-16 Rhone-Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the same
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ES2018274T5 (en) 1986-03-11 1996-12-16 Plant Genetic Systems Nv VEGETABLE CELLS RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS, PREPARED BY GENETIC ENGINEERING.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
EP0528819A1 (en) 1990-04-18 1993-03-03 Plant Genetic Systems, N.V. Modified bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
TW261517B (en) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US8877916B2 (en) * 2000-04-26 2014-11-04 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
EP1349917B1 (en) * 2000-12-08 2010-03-10 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2007500514A (en) 2003-04-29 2007-01-18 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド Novel glyphosate N-acetyltransferase (GAT) gene
NZ542933A (en) * 2003-05-05 2009-08-28 Dow Agrosciences Llc Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
CN1984558B (en) 2004-04-30 2011-01-12 美国陶氏益农公司 New herbicide resistance gene
DK1947926T3 (en) 2005-10-28 2015-01-26 Dow Agrosciences Llc new herbicide resistant genes

Also Published As

Publication number Publication date
IL236814A0 (en) 2015-03-31
EP2877581B1 (en) 2017-11-29
EP2877581A1 (en) 2015-06-03
US20150176015A1 (en) 2015-06-25
CN104619847A (en) 2015-05-13
WO2014018512A1 (en) 2014-01-30
KR20150032306A (en) 2015-03-25
CA2882143A1 (en) 2014-01-30
US9752154B2 (en) 2017-09-05
AU2013293123A1 (en) 2015-01-22
AU2013293123B2 (en) 2018-04-05
JP2015525567A (en) 2015-09-07
ZA201409509B (en) 2016-08-31
BR112015001316A2 (en) 2017-08-01
RU2015106549A (en) 2016-09-20

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