JP2015525567A - ハイスループットｄｎａフラグメントアセンブリ - Google Patents

ハイスループットｄｎａフラグメントアセンブリ Download PDF

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Publication number
JP2015525567A
JP2015525567A JP2015524385A JP2015524385A JP2015525567A JP 2015525567 A JP2015525567 A JP 2015525567A JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015524385 A JP2015524385 A JP 2015524385A JP 2015525567 A JP2015525567 A JP 2015525567A
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JP
Japan
Prior art keywords
sequence
dna
dna molecule
vector
restriction enzyme
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JP2015524385A
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English (en)
Japanese (ja)
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JP2015525567A5 (enExample
Inventor
クマール，サンディープ
エバンス，スティーブン，エル．
グプタ，マンジュ
Original Assignee
ダウ アグロサイエンシィズ エルエルシー
ダウ アグロサイエンシィズ エルエルシー
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Application filed by ダウ アグロサイエンシィズ エルエルシー, ダウ アグロサイエンシィズ エルエルシー filed Critical ダウ アグロサイエンシィズ エルエルシー
Publication of JP2015525567A publication Critical patent/JP2015525567A/ja
Publication of JP2015525567A5 publication Critical patent/JP2015525567A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015524385A 2012-07-26 2013-07-23 ハイスループットｄｎａフラグメントアセンブリ Pending JP2015525567A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261675929P 2012-07-26 2012-07-26
US61/675,929 2012-07-26
PCT/US2013/051641 WO2014018512A1 (en) 2012-07-26 2013-07-23 High-throughput dna fragment assembly

Publications (2)

Publication Number Publication Date
JP2015525567A true JP2015525567A (ja) 2015-09-07
JP2015525567A5 JP2015525567A5 (enExample) 2016-06-23

Family

ID=48914467

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015524385A Pending JP2015525567A (ja) 2012-07-26 2013-07-23 ハイスループットｄｎａフラグメントアセンブリ

Country Status (13)

Country Link
US (1) US9752154B2 (enExample)
EP (1) EP2877581B1 (enExample)
JP (1) JP2015525567A (enExample)
KR (1) KR20150032306A (enExample)
CN (1) CN104619847A (enExample)
AU (1) AU2013293123B2 (enExample)
BR (1) BR112015001316A2 (enExample)
CA (1) CA2882143A1 (enExample)
IL (1) IL236814A0 (enExample)
IN (1) IN2014DN11250A (enExample)
RU (1) RU2015106549A (enExample)
WO (1) WO2014018512A1 (enExample)
ZA (1) ZA201409509B (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002150B (zh) * 2015-01-09 2020-09-01 深圳华大基因研究院 一种dna合成产物的高通量检测方法
CN106318961B (zh) * 2015-06-30 2019-07-09 未名生物农业集团有限公司 一种多dna片段载体的构建方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004522428A (ja) * 2000-12-08 2004-07-29 インヴィトロジェン コーポレーション 複数の認識部位を用いた核酸分子合成のための方法および組成物

Family Cites Families (18)

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US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
EP0290455A4 (en) 1986-01-08 1989-01-18 Rhone Poulenc Agrochimie HALOARYLNITRILE DEGREASING GENE, THE USE THEREOF AND THE CELLS THAT CONTAIN IT.
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
JPH05506578A (ja) 1990-04-18 1993-09-30 プラント・ジエネテイツク・システムズ・エヌ・ベー 変性ｂａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
TW261517B (enExample) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US8877916B2 (en) * 2000-04-26 2014-11-04 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
JP2007526215A (ja) * 2003-05-05 2007-09-13 ダウ アグロサイエンス リミテッド ライアビリティー カンパニー トランスジェニック植物細胞に由来する安定な免疫予防的および治療的組成物ならびにその産生方法
CA2897475C (en) 2004-04-30 2018-07-10 Dow Agrosciences Llc Novel herbicide resistance genes
EP3241430B1 (en) 2005-10-28 2020-08-26 Dow AgroSciences LLC Novel herbicide resistance genes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004522428A (ja) * 2000-12-08 2004-07-29 インヴィトロジェン コーポレーション 複数の認識部位を用いた核酸分子合成のための方法および組成物

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BMC MOLECULAR BIOLOGY, 2006, 7:46, JPN6017014731, ISSN: 0003544905 *
TRENDS IN PLANT SCIENCE, 2005, VOL.10, P.103-105, JPN6017014732, ISSN: 0003544906 *

Also Published As

Publication number Publication date
ZA201409509B (en) 2016-08-31
BR112015001316A2 (pt) 2017-08-01
EP2877581A1 (en) 2015-06-03
CA2882143A1 (en) 2014-01-30
US20150176015A1 (en) 2015-06-25
US9752154B2 (en) 2017-09-05
RU2015106549A (ru) 2016-09-20
AU2013293123B2 (en) 2018-04-05
EP2877581B1 (en) 2017-11-29
KR20150032306A (ko) 2015-03-25
IL236814A0 (en) 2015-03-31
IN2014DN11250A (enExample) 2015-10-09
AU2013293123A1 (en) 2015-01-22
WO2014018512A1 (en) 2014-01-30
CN104619847A (zh) 2015-05-13

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