CA2882143A1 - High-throughput dna fragment assembly - Google Patents
High-throughput dna fragment assembly Download PDFInfo
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- CA2882143A1 CA2882143A1 CA2882143A CA2882143A CA2882143A1 CA 2882143 A1 CA2882143 A1 CA 2882143A1 CA 2882143 A CA2882143 A CA 2882143A CA 2882143 A CA2882143 A CA 2882143A CA 2882143 A1 CA2882143 A1 CA 2882143A1
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- dna
- sequence
- vector
- dna molecule
- restriction enzyme
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| US201261675929P | 2012-07-26 | 2012-07-26 | |
| US61/675,929 | 2012-07-26 | ||
| PCT/US2013/051641 WO2014018512A1 (en) | 2012-07-26 | 2013-07-23 | High-throughput dna fragment assembly |
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| CA2882143A1 true CA2882143A1 (en) | 2014-01-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107002150A (zh) * | 2015-01-09 | 2017-08-01 | 深圳华大基因研究院 | 一种dna 合成产物的高通量检测方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106318961B (zh) * | 2015-06-30 | 2019-07-09 | 未名生物农业集团有限公司 | 一种多dna片段载体的构建方法及其应用 |
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| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| EP0290455A4 (en) | 1986-01-08 | 1989-01-18 | Rhone Poulenc Agrochimie | HALOARYLNITRILE DEGREASING GENE, THE USE THEREOF AND THE CELLS THAT CONTAIN IT. |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ATE57390T1 (de) | 1986-03-11 | 1990-10-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| JPH05506578A (ja) | 1990-04-18 | 1993-09-30 | プラント・ジエネテイツク・システムズ・エヌ・ベー | 変性ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現 |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| TW261517B (enExample) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8877916B2 (en) * | 2000-04-26 | 2014-11-04 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| CA2430947A1 (en) * | 2000-12-08 | 2002-06-13 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| CN1863914B (zh) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | 新的草甘膦-n-乙酰转移酶(gat)基因 |
| JP2007526215A (ja) * | 2003-05-05 | 2007-09-13 | ダウ アグロサイエンス リミテッド ライアビリティー カンパニー | トランスジェニック植物細胞に由来する安定な免疫予防的および治療的組成物ならびにその産生方法 |
| CA2897475C (en) | 2004-04-30 | 2018-07-10 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| EP3241430B1 (en) | 2005-10-28 | 2020-08-26 | Dow AgroSciences LLC | Novel herbicide resistance genes |
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2013
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107002150A (zh) * | 2015-01-09 | 2017-08-01 | 深圳华大基因研究院 | 一种dna 合成产物的高通量检测方法 |
| CN107002150B (zh) * | 2015-01-09 | 2020-09-01 | 深圳华大基因研究院 | 一种dna合成产物的高通量检测方法 |
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| Publication number | Publication date |
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| ZA201409509B (en) | 2016-08-31 |
| BR112015001316A2 (pt) | 2017-08-01 |
| JP2015525567A (ja) | 2015-09-07 |
| EP2877581A1 (en) | 2015-06-03 |
| US20150176015A1 (en) | 2015-06-25 |
| US9752154B2 (en) | 2017-09-05 |
| RU2015106549A (ru) | 2016-09-20 |
| AU2013293123B2 (en) | 2018-04-05 |
| EP2877581B1 (en) | 2017-11-29 |
| KR20150032306A (ko) | 2015-03-25 |
| IL236814A0 (en) | 2015-03-31 |
| IN2014DN11250A (enExample) | 2015-10-09 |
| AU2013293123A1 (en) | 2015-01-22 |
| WO2014018512A1 (en) | 2014-01-30 |
| CN104619847A (zh) | 2015-05-13 |
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Effective date: 20190723 |