KR20150032306A - 고 처리량 dna 단편 조립 - Google Patents
고 처리량 dna 단편 조립 Download PDFInfo
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- KR20150032306A KR20150032306A KR1020157001623A KR20157001623A KR20150032306A KR 20150032306 A KR20150032306 A KR 20150032306A KR 1020157001623 A KR1020157001623 A KR 1020157001623A KR 20157001623 A KR20157001623 A KR 20157001623A KR 20150032306 A KR20150032306 A KR 20150032306A
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
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- C12N15/67—General methods for enhancing the expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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| CN106318961B (zh) * | 2015-06-30 | 2019-07-09 | 未名生物农业集团有限公司 | 一种多dna片段载体的构建方法及其应用 |
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| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| IL81168A0 (en) | 1986-01-08 | 1987-08-31 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene,its use,and cells containing the same |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ES2018274T5 (es) | 1986-03-11 | 1996-12-16 | Plant Genetic Systems Nv | Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| EP0528819A1 (en) | 1990-04-18 | 1993-03-03 | Plant Genetic Systems, N.V. | Modified bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| TW261517B (enExample) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8877916B2 (en) * | 2000-04-26 | 2014-11-04 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| WO2005012515A2 (en) | 2003-04-29 | 2005-02-10 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
| WO2002046372A1 (en) * | 2000-12-08 | 2002-06-13 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| JP2007526215A (ja) * | 2003-05-05 | 2007-09-13 | ダウ アグロサイエンス リミテッド ライアビリティー カンパニー | トランスジェニック植物細胞に由来する安定な免疫予防的および治療的組成物ならびにその産生方法 |
| SI2308976T1 (sl) | 2004-04-30 | 2013-05-31 | Dow Agrosciences Llc | Novi geni z rezistenco na herbicide |
| EP2484202B1 (en) | 2005-10-28 | 2017-06-07 | Dow AgroSciences LLC | Novel herbicide resistance genes |
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| US9752154B2 (en) | 2017-09-05 |
| AU2013293123A1 (en) | 2015-01-22 |
| CA2882143A1 (en) | 2014-01-30 |
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| EP2877581B1 (en) | 2017-11-29 |
| CN104619847A (zh) | 2015-05-13 |
| RU2015106549A (ru) | 2016-09-20 |
| WO2014018512A1 (en) | 2014-01-30 |
| BR112015001316A2 (pt) | 2017-08-01 |
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| JP2015525567A (ja) | 2015-09-07 |
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| AU2013293123B2 (en) | 2018-04-05 |
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