AU2013293123B2 - High-throughput DNA fragment assembly - Google Patents

High-throughput DNA fragment assembly Download PDF

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Publication number
AU2013293123B2
AU2013293123B2 AU2013293123A AU2013293123A AU2013293123B2 AU 2013293123 B2 AU2013293123 B2 AU 2013293123B2 AU 2013293123 A AU2013293123 A AU 2013293123A AU 2013293123 A AU2013293123 A AU 2013293123A AU 2013293123 B2 AU2013293123 B2 AU 2013293123B2
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AU
Australia
Prior art keywords
dna
sequence
seq
artificial sequence
kozak
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2013293123A
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English (en)
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AU2013293123A1 (en
Inventor
Steven L. Evans
Manju Gupta
Sandeep Kumar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corteva Agriscience LLC
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Corteva Agriscience LLC
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Publication of AU2013293123A1 publication Critical patent/AU2013293123A1/en
Assigned to DOW AGROSCIENCES LLC reassignment DOW AGROSCIENCES LLC Amend patent request/document other than specification (104) Assignors: DOW AGROSCIENCES LLC, MANJU GUPTA, SANDEEP KUMAR, STEVEN L. EVANS
Application granted granted Critical
Publication of AU2013293123B2 publication Critical patent/AU2013293123B2/en
Assigned to CORTEVA AGRISCIENCE LLC reassignment CORTEVA AGRISCIENCE LLC Request to Amend Deed and Register Assignors: DOW AGROSCIENCES, LLC
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2013293123A 2012-07-26 2013-07-23 High-throughput DNA fragment assembly Ceased AU2013293123B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261675929P 2012-07-26 2012-07-26
US61/675,929 2012-07-26
PCT/US2013/051641 WO2014018512A1 (en) 2012-07-26 2013-07-23 High-throughput dna fragment assembly

Publications (2)

Publication Number Publication Date
AU2013293123A1 AU2013293123A1 (en) 2015-01-22
AU2013293123B2 true AU2013293123B2 (en) 2018-04-05

Family

ID=48914467

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013293123A Ceased AU2013293123B2 (en) 2012-07-26 2013-07-23 High-throughput DNA fragment assembly

Country Status (13)

Country Link
US (1) US9752154B2 (enExample)
EP (1) EP2877581B1 (enExample)
JP (1) JP2015525567A (enExample)
KR (1) KR20150032306A (enExample)
CN (1) CN104619847A (enExample)
AU (1) AU2013293123B2 (enExample)
BR (1) BR112015001316A2 (enExample)
CA (1) CA2882143A1 (enExample)
IL (1) IL236814A0 (enExample)
IN (1) IN2014DN11250A (enExample)
RU (1) RU2015106549A (enExample)
WO (1) WO2014018512A1 (enExample)
ZA (1) ZA201409509B (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002150B (zh) * 2015-01-09 2020-09-01 深圳华大基因研究院 一种dna合成产物的高通量检测方法
CN106318961B (zh) * 2015-06-30 2019-07-09 未名生物农业集团有限公司 一种多dna片段载体的构建方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229229A1 (en) * 1999-12-10 2004-11-18 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US20120084885A1 (en) * 2000-04-26 2012-04-05 Nickolai Alexandrov Promoter, promoter control elements, and combinations, and uses thereof

Family Cites Families (17)

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US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
WO1987004181A1 (en) 1986-01-08 1987-07-16 Rhone-Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the same
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
EP0528819A1 (en) 1990-04-18 1993-03-03 Plant Genetic Systems, N.V. Modified bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
TW261517B (enExample) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
EP1349917B1 (en) * 2000-12-08 2010-03-10 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2007500514A (ja) 2003-04-29 2007-01-18 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド 新規なグリホセートｎ−アセチルトランスフェラーゼ（ｇａｔ）遺伝子
NZ542933A (en) * 2003-05-05 2009-08-28 Dow Agrosciences Llc Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
CN1984558B (zh) 2004-04-30 2011-01-12 美国陶氏益农公司 新除草剂抗性基因
DK1947926T3 (en) 2005-10-28 2015-01-26 Dow Agrosciences Llc new herbicide resistant genes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229229A1 (en) * 1999-12-10 2004-11-18 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US20120084885A1 (en) * 2000-04-26 2012-04-05 Nickolai Alexandrov Promoter, promoter control elements, and combinations, and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Li et al., Nature Methods, 2007, vol 4, pages 251-256 *

Also Published As

Publication number Publication date
IL236814A0 (en) 2015-03-31
EP2877581B1 (en) 2017-11-29
EP2877581A1 (en) 2015-06-03
IN2014DN11250A (enExample) 2015-10-09
US20150176015A1 (en) 2015-06-25
CN104619847A (zh) 2015-05-13
WO2014018512A1 (en) 2014-01-30
KR20150032306A (ko) 2015-03-25
CA2882143A1 (en) 2014-01-30
US9752154B2 (en) 2017-09-05
AU2013293123A1 (en) 2015-01-22
JP2015525567A (ja) 2015-09-07
ZA201409509B (en) 2016-08-31
BR112015001316A2 (pt) 2017-08-01
RU2015106549A (ru) 2016-09-20

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