CN107002150A - 一种dna 合成产物的高通量检测方法 - Google Patents

一种dna 合成产物的高通量检测方法 Download PDF

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CN107002150A
CN107002150A CN201580068286.XA CN201580068286A CN107002150A CN 107002150 A CN107002150 A CN 107002150A CN 201580068286 A CN201580068286 A CN 201580068286A CN 107002150 A CN107002150 A CN 107002150A
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dna
clone
sequencing
mixing
sequence
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CN107002150B (zh
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康康
陈世宏
沈玥
王云
徐讯
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
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  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了一种DNA合成产物的高通量检测方法，包括如下步骤：将多个DNA组件分别连入克隆载体并转入筛选菌株中，筛选出连接有DNA组件的克隆；分别对克隆进行编号，并培养出相应克隆菌液；选取编号相同的克隆菌液混合并提取质粒；连接测序标签，构建多个混合测序文库，其中不同混合测序文库的测序标签不同；进行混样高通量测序，得到DNA组件的序列；与参考序列进行比对，得到DNA合成产物中具有设定正确率的目标DNA组件。本发明的方法通过混合文库构建策略，将高通量测序技术运用于DNA芯片合成产物的检测，降低产物检测和正确产物筛选成本。

Description

PCT国内申请，说明书已公开。

Claims (12)

  1. PCT国内申请，权利要求书已公开。
CN201580068286.XA 2015-01-09 2015-01-09 一种dna合成产物的高通量检测方法 Active CN107002150B (zh)

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PCT/CN2015/070459 WO2016109981A1 (zh) 2015-01-09 2015-01-09 一种dna合成产物的高通量检测方法

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108866171A (zh) * 2017-05-10 2018-11-23 深圳华大基因研究院 一种基于新一代测序的古生物物种鉴定方法
CN109517882B (zh) * 2018-11-09 2021-08-17 广州燃石医学检验所有限公司 一种用于检测独特双端文库标签组合的质控方法及应用
CN111926393A (zh) * 2019-05-13 2020-11-13 苏州金唯智生物科技有限公司 一种测序文库的构建方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1251615A (zh) * 1997-02-14 2000-04-26 乔治华盛顿大学 测量dna合成率的分析方法
CA2882143A1 (en) * 2012-07-26 2014-01-30 Dow Agrosciences Llc High-throughput dna fragment assembly

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1251615A (zh) * 1997-02-14 2000-04-26 乔治华盛顿大学 测量dna合成率的分析方法
CA2882143A1 (en) * 2012-07-26 2014-01-30 Dow Agrosciences Llc High-throughput dna fragment assembly

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US10179934B2 (en) 2019-01-15
WO2016109981A1 (zh) 2016-07-14
US20170349945A1 (en) 2017-12-07
CN107002150B (zh) 2020-09-01

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