PT1078051E - Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas - Google Patents

Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas Download PDF

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Publication number
PT1078051E
PT1078051E PT99922306T PT99922306T PT1078051E PT 1078051 E PT1078051 E PT 1078051E PT 99922306 T PT99922306 T PT 99922306T PT 99922306 T PT99922306 T PT 99922306T PT 1078051 E PT1078051 E PT 1078051E
Authority
PT
Portugal
Prior art keywords
virus
selection
polypeptide
phage
display system
Prior art date
Application number
PT99922306T
Other languages
English (en)
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of PT1078051E publication Critical patent/PT1078051E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Circuits Of Receivers In General (AREA)
  • Vehicle Body Suspensions (AREA)
  • Air Bags (AREA)
PT99922306T 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas PT1078051E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method

Publications (1)

Publication Number Publication Date
PT1078051E true PT1078051E (pt) 2008-03-17

Family

ID=26313668

Family Applications (1)

Application Number Title Priority Date Filing Date
PT99922306T PT1078051E (pt) 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas

Country Status (14)

Country Link
US (2) US7442159B1 (pt)
EP (2) EP1078051B1 (pt)
JP (2) JP4493846B2 (pt)
AT (1) ATE380865T1 (pt)
AU (1) AU762814B2 (pt)
CA (1) CA2328422A1 (pt)
CY (1) CY1107293T1 (pt)
DE (1) DE69937735T2 (pt)
DK (1) DK1078051T3 (pt)
ES (1) ES2299244T3 (pt)
GB (1) GB2361237A (pt)
NO (1) NO329172B1 (pt)
PT (1) PT1078051E (pt)
WO (1) WO1999058655A2 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002522029A (ja) 1998-07-27 2002-07-23 ジェネンテック・インコーポレーテッド コートタンパク質の改変によるファージ提示における改良した形質転換効率
EP1990409A3 (en) 1999-07-20 2011-05-18 MorphoSys AG Bacteriophage
WO2001057065A2 (en) * 2000-02-03 2001-08-09 Domantis Limited Combinatorial protein domains
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
AU2001277614A1 (en) * 2000-08-07 2002-02-18 Domantis Limited Novel proteins
DE10135039C1 (de) * 2001-07-18 2003-03-13 Nemod Immuntherapie Ag Verfahren zur Isolierung großer Varianzen spezifischer Moleküle für ein Zielmolekül aus Phagemid-Gen-Bibliotheken
EP1627062A1 (en) 2003-05-14 2006-02-22 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
CA2614512C (en) 2005-07-08 2014-01-07 University Of Zuerich Phage display using cotranslational translocation of fusion polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
DK1915617T3 (da) * 2005-07-21 2011-11-21 Aebi Ltd Fremgangsmåder og sammensætninger til identifikation af et peptid, der har en intermolekylær interaktion med et interessemål
GB0515115D0 (en) 2005-07-22 2005-08-31 Isogenica Ltd Peptide characterisation
ES2444695T3 (es) * 2006-06-23 2014-02-26 Alethia Biotherapeutics Inc. Polinucleótidos y polipéptidos implicados en el cáncer
MX2009013137A (es) 2007-06-06 2010-04-30 Domantis Ltd Metodos para seleccionar polipeptidos resistentes a la proteasa.
JP2011504722A (ja) 2007-08-21 2011-02-17 モルフォシス・アー・ゲー ジスルフィド結合形成のための改良された方法
US20110008345A1 (en) 2007-11-30 2011-01-13 Claire Ashman Antigen-binding constructs
CN104829714A (zh) 2008-11-03 2015-08-12 阿莱斯亚生物疗法股份有限公司 特异性地阻滞肿瘤抗原的生物活性的抗体
MX2011005874A (es) 2008-12-05 2011-06-27 Glaxo Group Ltd Metodos para seleccionar polipeptidos resistentes a proteasa.
JP2012518398A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
JP2012518399A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
EP2643351A1 (en) 2010-11-24 2013-10-02 Glaxo Group Limited Multispecific antigen binding proteins targeting hgf
DK3173427T3 (da) 2011-03-31 2019-08-05 Adc Therapeutics Sa Antistoffer mod nyre-associeret antigen 1 og antigen-bindende fragmenter deraf
US9499612B2 (en) 2011-07-27 2016-11-22 Glaxo Group Limited Antigen binding constructs
EP3431589A1 (en) 2011-10-27 2019-01-23 The Governors of the University of Alberta Device and method for bacterial culture and assay
ES2731665T3 (es) 2012-01-09 2019-11-18 Adc Therapeutics Sa Agentes para tratar cáncer de mama triple negativo
AU2014324884B2 (en) * 2013-09-25 2020-03-26 Cytomx Therapeutics, Inc Matrix metalloproteinase substrates and other cleavable moieties and methods of use thereof
WO2015116933A2 (en) 2014-01-31 2015-08-06 Cytomx Therapeutics, Inc. Matriptase and u-plasminogen activator substrates and other cleavable moieties and methods of use thereof
US20180340174A1 (en) * 2014-11-11 2018-11-29 Nanocore Aps Method for identification of molecules with desired characteristics
MA41374A (fr) 2015-01-20 2017-11-28 Cytomx Therapeutics Inc Substrats clivables par métalloprotéase matricielle et clivables par sérine protéase et procédés d'utilisation de ceux-ci
IL254589B2 (en) * 2015-03-31 2023-11-01 Vhsquared Ltd A peptide structure with a protease-cleavable linker
US20220228172A1 (en) * 2019-06-21 2022-07-21 Boehringer Ingelheim International Gmbh Novel Mechanism to Control RNA Virus Replication and Gene Expression

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
AU634186B2 (en) 1988-11-11 1993-02-18 Medical Research Council Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
WO1992020791A1 (en) 1990-07-10 1992-11-26 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
ES2330052T3 (es) * 1991-03-01 2009-12-03 Dyax Corporation Proteina quimerica que comprende micro-proteinas que tienen dos o mas puentes disulfuro y relaizaciones de las mismas.
DE4122599C2 (de) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
ES2227512T3 (es) 1991-12-02 2005-04-01 Medical Research Council Produccion de anticuerpos contra auto-antigenos a partir de repertorios de segmentos de anticuerpos fijados en un fago.

Also Published As

Publication number Publication date
AU3941399A (en) 1999-11-29
EP1078051B1 (en) 2007-12-12
CY1107293T1 (el) 2012-11-21
EP1078051A2 (en) 2001-02-28
JP2002514413A (ja) 2002-05-21
GB2361237A (en) 2001-10-17
US8680018B2 (en) 2014-03-25
ATE380865T1 (de) 2007-12-15
WO1999058655A3 (en) 2000-01-27
AU762814B2 (en) 2003-07-03
JP4493846B2 (ja) 2010-06-30
GB0026143D0 (en) 2000-12-13
NO20005628L (no) 2001-01-12
JP2010148507A (ja) 2010-07-08
DK1078051T3 (da) 2008-04-07
WO1999058655A2 (en) 1999-11-18
EP1950295A1 (en) 2008-07-30
DE69937735T2 (de) 2008-11-27
US7442159B1 (en) 2008-10-28
CA2328422A1 (en) 1999-11-18
NO329172B1 (no) 2010-09-06
US20090203542A1 (en) 2009-08-13
DE69937735D1 (de) 2008-01-24
ES2299244T3 (es) 2008-05-16
JP5161865B2 (ja) 2013-03-13
NO20005628D0 (no) 2000-11-08

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