DE69937735D1 - Phagen-display-selektionssystem für korrekt gefaltete proteine - Google Patents
Phagen-display-selektionssystem für korrekt gefaltete proteineInfo
- Publication number
- DE69937735D1 DE69937735D1 DE69937735T DE69937735T DE69937735D1 DE 69937735 D1 DE69937735 D1 DE 69937735D1 DE 69937735 T DE69937735 T DE 69937735T DE 69937735 T DE69937735 T DE 69937735T DE 69937735 D1 DE69937735 D1 DE 69937735D1
- Authority
- DE
- Germany
- Prior art keywords
- virus
- polypeptide
- phag
- selection system
- display selection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Control Of Indicators Other Than Cathode Ray Tubes (AREA)
- Saccharide Compounds (AREA)
- Vehicle Body Suspensions (AREA)
- Circuits Of Receivers In General (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Air Bags (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9810223.9A GB9810223D0 (en) | 1998-05-13 | 1998-05-13 | Selection system |
GBGB9810228.8A GB9810228D0 (en) | 1998-05-13 | 1998-05-13 | Selection method |
GB9810228 | 1998-05-13 | ||
GB9810223 | 1998-05-13 | ||
PCT/GB1999/001526 WO1999058655A2 (en) | 1998-05-13 | 1999-05-13 | Phage display selection system for folded proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69937735D1 true DE69937735D1 (de) | 2008-01-24 |
DE69937735T2 DE69937735T2 (de) | 2008-11-27 |
Family
ID=26313668
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69937735T Expired - Lifetime DE69937735T2 (de) | 1998-05-13 | 1999-05-13 | Phagen-display-selektionssystem für korrekt gefaltete proteine |
Country Status (14)
Country | Link |
---|---|
US (2) | US7442159B1 (de) |
EP (2) | EP1950295A1 (de) |
JP (2) | JP4493846B2 (de) |
AT (1) | ATE380865T1 (de) |
AU (1) | AU762814B2 (de) |
CA (1) | CA2328422A1 (de) |
CY (1) | CY1107293T1 (de) |
DE (1) | DE69937735T2 (de) |
DK (1) | DK1078051T3 (de) |
ES (1) | ES2299244T3 (de) |
GB (1) | GB2361237A (de) |
NO (1) | NO329172B1 (de) |
PT (1) | PT1078051E (de) |
WO (1) | WO1999058655A2 (de) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1100890A2 (de) | 1998-07-27 | 2001-05-23 | Genentech, Inc. | Verbesserung der transformationseffizienz in phagendisplay durch abänderung des hüllproteins |
PT1144607E (pt) | 1999-07-20 | 2009-04-22 | Morphosys Ag | Novos métodos para apresentar (poli)peptídeos/ proteínas em partículas bacteriofágicas através de ligações dissulfureto |
AU3040101A (en) * | 2000-02-03 | 2001-08-14 | Domantis Limited | Combinatorial protein domains |
GB0019049D0 (en) * | 2000-08-04 | 2000-09-27 | Neutec Pharma Plc | Combinatorial chemistry method |
WO2002012277A2 (en) * | 2000-08-07 | 2002-02-14 | Domantis Limited | Hybrid combinatorial proteins made from reshuffling of differently folded domains |
DE10135039C1 (de) * | 2001-07-18 | 2003-03-13 | Nemod Immuntherapie Ag | Verfahren zur Isolierung großer Varianzen spezifischer Moleküle für ein Zielmolekül aus Phagemid-Gen-Bibliotheken |
WO2004101790A1 (en) | 2003-05-14 | 2004-11-25 | Domantis Limited | A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire |
AU2006268780B2 (en) * | 2005-07-08 | 2011-03-24 | University Of Zurich | Phage display using cotranslational translocation of fusion polypeptides |
GB2428293A (en) * | 2005-07-13 | 2007-01-24 | Domantis Ltd | Phage display libraries |
EP1915617B1 (de) | 2005-07-21 | 2011-09-14 | Aebi Ltd. | Verfahren und zusammensetzungen zur identifizierung eines peptids mit intermolekularer wechselwirkung mit einem interessierenden ziel |
GB0515115D0 (en) | 2005-07-22 | 2005-08-31 | Isogenica Ltd | Peptide characterisation |
EP2032701B1 (de) * | 2006-06-23 | 2013-11-27 | Alethia Biotherapeutics Inc. | An Krebs beteiligte Polynukleotide und Polypeptide |
CA2688433A1 (en) | 2007-06-06 | 2008-12-11 | Domantis Limited | Methods for selecting protease resistant polypeptides |
CN101772574B (zh) | 2007-08-21 | 2016-01-06 | 莫佛塞斯公司 | 改良的用于二硫键形成的方法 |
ES2614284T3 (es) | 2007-11-30 | 2017-05-30 | Glaxo Group Limited | Construcciones de unión a antígenos |
MX336869B (es) | 2008-11-03 | 2016-02-04 | Alethia Biotherapeutics Inc | Anticuerpos que bloquean espicificamente la actividad biologica de un antigeno de tumor. |
WO2010063818A2 (en) | 2008-12-05 | 2010-06-10 | Glaxo Group Limited | Methods for selecting protease resistant polypeptides |
EP2401297A1 (de) | 2009-02-24 | 2012-01-04 | Glaxo Group Limited | Antigenbindende konstrukte |
WO2010097394A1 (en) | 2009-02-24 | 2010-09-02 | Glaxo Group Limited | Multivalent and/or multispecific rankl-binding constructs |
CA2753287A1 (en) | 2009-02-24 | 2010-09-02 | Glaxo Group Limited | Antigen-binding constructs |
US20130236467A1 (en) | 2010-11-24 | 2013-09-12 | Jeremy Griggs | Multispecific antigen binding proteins targeting hgf |
MX352373B (es) | 2011-03-31 | 2017-11-22 | Adc Therapeutics Sa | Anticuerpos contra antigeno 1 asociado con riñon y sus fragmentos de union de antigeno. |
BR112014001855A2 (pt) | 2011-07-27 | 2017-02-21 | Glaxo Group Ltd | construto e proteína aglutinantes ao antígeno, dímero, composição farmacêutica, sequência de polinucleotídeo, célula hospedeira e, método para produção do construto |
AU2012327806B2 (en) | 2011-10-27 | 2018-07-19 | The Governors Of The University Of Alberta | Device and method for bacterial culture and assay |
CN111298119A (zh) | 2012-01-09 | 2020-06-19 | Adc治疗股份有限公司 | 用于通过使用肾相关抗原1(kaag1)的抑制剂来治疗三阴性乳癌和基底样乳癌的方法 |
CN106163556B (zh) | 2013-09-25 | 2024-04-09 | 西托姆克斯治疗公司 | 基质金属蛋白酶底物和其它可切割部分及其使用方法 |
NZ722401A (en) | 2014-01-31 | 2023-06-30 | Cytomx Therapeutics Inc | Matriptase and u-plasminogen activator substrates and other cleavable moieties and methods of use thereof |
EP3218479A1 (de) * | 2014-11-11 | 2017-09-20 | Nanocore ApS | Verfahren zur identifizierung von molekülen mit gewünschten eigenschaften |
MA41374A (fr) | 2015-01-20 | 2017-11-28 | Cytomx Therapeutics Inc | Substrats clivables par métalloprotéase matricielle et clivables par sérine protéase et procédés d'utilisation de ceux-ci |
SG11201706840WA (en) * | 2015-03-31 | 2017-09-28 | Vhsquared Ltd | Peptide construct having a protease-cleavable linker |
WO2020254644A1 (en) * | 2019-06-21 | 2020-12-24 | Boehringer Ingelheim International Gmbh | Novel mechanism to control rna virus replication and gene expression |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
WO1990005144A1 (en) | 1988-11-11 | 1990-05-17 | Medical Research Council | Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors |
CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
US5723286A (en) * | 1990-06-20 | 1998-03-03 | Affymax Technologies N.V. | Peptide library and screening systems |
GB9015198D0 (en) * | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
ES2139598T3 (es) | 1990-07-10 | 2000-02-16 | Medical Res Council | Procedimientos para la produccion de miembros de parejas de union especifica. |
JP4146512B2 (ja) * | 1991-03-01 | 2008-09-10 | ダイアックス コープ. | 小型タンパク質 |
DE4122599C2 (de) * | 1991-07-08 | 1993-11-11 | Deutsches Krebsforsch | Phagemid zum Screenen von Antikörpern |
ES2227512T3 (es) | 1991-12-02 | 2005-04-01 | Medical Research Council | Produccion de anticuerpos contra auto-antigenos a partir de repertorios de segmentos de anticuerpos fijados en un fago. |
-
1999
- 1999-05-13 CA CA002328422A patent/CA2328422A1/en not_active Abandoned
- 1999-05-13 GB GB0026143A patent/GB2361237A/en not_active Withdrawn
- 1999-05-13 EP EP07023393A patent/EP1950295A1/de not_active Ceased
- 1999-05-13 AT AT99922306T patent/ATE380865T1/de active
- 1999-05-13 EP EP99922306A patent/EP1078051B1/de not_active Expired - Lifetime
- 1999-05-13 JP JP2000548446A patent/JP4493846B2/ja not_active Expired - Lifetime
- 1999-05-13 AU AU39413/99A patent/AU762814B2/en not_active Expired
- 1999-05-13 WO PCT/GB1999/001526 patent/WO1999058655A2/en active IP Right Grant
- 1999-05-13 ES ES99922306T patent/ES2299244T3/es not_active Expired - Lifetime
- 1999-05-13 DE DE69937735T patent/DE69937735T2/de not_active Expired - Lifetime
- 1999-05-13 PT PT99922306T patent/PT1078051E/pt unknown
- 1999-05-13 DK DK99922306T patent/DK1078051T3/da active
-
2000
- 2000-11-08 NO NO20005628A patent/NO329172B1/no not_active IP Right Cessation
- 2000-11-10 US US09/710,444 patent/US7442159B1/en not_active Expired - Fee Related
-
2008
- 2008-02-29 CY CY20081100242T patent/CY1107293T1/el unknown
- 2008-10-07 US US12/246,741 patent/US8680018B2/en not_active Expired - Fee Related
-
2009
- 2009-12-25 JP JP2009294773A patent/JP5161865B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20090203542A1 (en) | 2009-08-13 |
CY1107293T1 (el) | 2012-11-21 |
ES2299244T3 (es) | 2008-05-16 |
ATE380865T1 (de) | 2007-12-15 |
EP1078051A2 (de) | 2001-02-28 |
JP2002514413A (ja) | 2002-05-21 |
US7442159B1 (en) | 2008-10-28 |
EP1078051B1 (de) | 2007-12-12 |
GB2361237A (en) | 2001-10-17 |
GB0026143D0 (en) | 2000-12-13 |
JP5161865B2 (ja) | 2013-03-13 |
US8680018B2 (en) | 2014-03-25 |
JP2010148507A (ja) | 2010-07-08 |
JP4493846B2 (ja) | 2010-06-30 |
EP1950295A1 (de) | 2008-07-30 |
NO20005628D0 (no) | 2000-11-08 |
WO1999058655A2 (en) | 1999-11-18 |
NO20005628L (no) | 2001-01-12 |
CA2328422A1 (en) | 1999-11-18 |
DK1078051T3 (da) | 2008-04-07 |
PT1078051E (pt) | 2008-03-17 |
WO1999058655A3 (en) | 2000-01-27 |
NO329172B1 (no) | 2010-09-06 |
DE69937735T2 (de) | 2008-11-27 |
AU3941399A (en) | 1999-11-29 |
AU762814B2 (en) | 2003-07-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |