DE69937735D1 - Phagen-display-selektionssystem für korrekt gefaltete proteine - Google Patents

Phagen-display-selektionssystem für korrekt gefaltete proteine

Info

Publication number
DE69937735D1
DE69937735D1 DE69937735T DE69937735T DE69937735D1 DE 69937735 D1 DE69937735 D1 DE 69937735D1 DE 69937735 T DE69937735 T DE 69937735T DE 69937735 T DE69937735 T DE 69937735T DE 69937735 D1 DE69937735 D1 DE 69937735D1
Authority
DE
Germany
Prior art keywords
virus
polypeptide
phag
selection system
display selection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69937735T
Other languages
English (en)
Other versions
DE69937735T2 (de
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Domantis Ltd
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Application granted granted Critical
Publication of DE69937735D1 publication Critical patent/DE69937735D1/de
Publication of DE69937735T2 publication Critical patent/DE69937735T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Saccharide Compounds (AREA)
  • Vehicle Body Suspensions (AREA)
  • Circuits Of Receivers In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Air Bags (AREA)
DE69937735T 1998-05-13 1999-05-13 Phagen-display-selektionssystem für korrekt gefaltete proteine Expired - Lifetime DE69937735T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB9810223 1998-05-13
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method
GB9810228 1998-05-13
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system
PCT/GB1999/001526 WO1999058655A2 (en) 1998-05-13 1999-05-13 Phage display selection system for folded proteins

Publications (2)

Publication Number Publication Date
DE69937735D1 true DE69937735D1 (de) 2008-01-24
DE69937735T2 DE69937735T2 (de) 2008-11-27

Family

ID=26313668

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69937735T Expired - Lifetime DE69937735T2 (de) 1998-05-13 1999-05-13 Phagen-display-selektionssystem für korrekt gefaltete proteine

Country Status (14)

Country Link
US (2) US7442159B1 (de)
EP (2) EP1950295A1 (de)
JP (2) JP4493846B2 (de)
AT (1) ATE380865T1 (de)
AU (1) AU762814B2 (de)
CA (1) CA2328422A1 (de)
CY (1) CY1107293T1 (de)
DE (1) DE69937735T2 (de)
DK (1) DK1078051T3 (de)
ES (1) ES2299244T3 (de)
GB (1) GB2361237A (de)
NO (1) NO329172B1 (de)
PT (1) PT1078051E (de)
WO (1) WO1999058655A2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006717A2 (en) 1998-07-27 2000-02-10 Genentech, Inc. Improved transformation efficiency in phage display through modification of a coat protein
PT1144607E (pt) 1999-07-20 2009-04-22 Morphosys Ag Novos métodos para apresentar (poli)peptídeos/ proteínas em partículas bacteriofágicas através de ligações dissulfureto
WO2001057065A2 (en) * 2000-02-03 2001-08-09 Domantis Limited Combinatorial protein domains
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
WO2002012277A2 (en) * 2000-08-07 2002-02-14 Domantis Limited Hybrid combinatorial proteins made from reshuffling of differently folded domains
DE10135039C1 (de) * 2001-07-18 2003-03-13 Nemod Immuntherapie Ag Verfahren zur Isolierung großer Varianzen spezifischer Moleküle für ein Zielmolekül aus Phagemid-Gen-Bibliotheken
EP2357237A1 (de) 2003-05-14 2011-08-17 Domantis Limited Verfahren zur gewinnung aus einem polypeptid - repertoire von polypeptiden, die sich reversibel entfalten
US8846577B2 (en) * 2005-07-08 2014-09-30 University Of Zurich Phage display using cotranslational translocation of fusion polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
ATE524579T1 (de) 2005-07-21 2011-09-15 Aebi Ltd Verfahren und zusammensetzungen zur identifizierung eines peptids mit intermolekularer wechselwirkung mit einem interessierenden ziel
GB0515115D0 (en) 2005-07-22 2005-08-31 Isogenica Ltd Peptide characterisation
DK2032701T3 (da) * 2006-06-23 2014-02-10 Alethia Biotherapeutics Inc Polynukleotider og polypeptider, der er inddraget i cancer
EA200901494A1 (ru) 2007-06-06 2010-06-30 Домантис Лимитед Способы селекции протеазоустойчивых полипептидов
EP2190987B1 (de) 2007-08-21 2012-11-14 MorphoSys AG Verfahren zur Formung von Disulphid-Bindungen
CN104650235A (zh) 2007-11-30 2015-05-27 葛兰素集团有限公司 抗原结合构建体
KR20110084280A (ko) 2008-11-03 2011-07-21 알레시아 바이오쎄라퓨틱스 인코포레이티드 종양 항원의 생물 활성을 특이적으로 차단하는 항체
AU2009324037B2 (en) 2008-12-05 2015-07-30 Glaxo Group Limited Methods for selecting protease resistant polypeptides
WO2010097385A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
JP2012518400A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 多価および／または複数特異的ｒａｎｋｌ結合性構築物
WO2010097386A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
JP2014501725A (ja) 2010-11-24 2014-01-23 グラクソ グループ リミテッド Ｈｇｆを標的とする多特異的抗原結合タンパク質
KR101993259B1 (ko) 2011-03-31 2019-06-27 에이디씨 테라퓨틱스 에스에이 신장 결합 항원 １에 대한 항체 및 이의 항원 결합 단편
US9499612B2 (en) 2011-07-27 2016-11-22 Glaxo Group Limited Antigen binding constructs
US9624523B2 (en) 2011-10-27 2017-04-18 The Governors Of The University Of Alberta Device and method for bacterial culture and assay
EA201992513A1 (ru) 2012-01-09 2020-05-31 Адс Терапьютикс Са Способ лечения рака груди
US20150087810A1 (en) * 2013-09-25 2015-03-26 Cytomx Therapeutics, Inc. Matrix Metalloproteinase Substrates And Other Cleavable Moieties And Methods Of Use Thereof
EP4176896A1 (de) 2014-01-31 2023-05-10 Cytomx Therapeutics Inc. Matriptase- und u-plasminogenaktivator-substrate und andere spaltbare teile und verfahren zur verwendung davon
EP3218479A1 (de) * 2014-11-11 2017-09-20 Nanocore ApS Verfahren zur identifizierung von molekülen mit gewünschten eigenschaften
MA41374A (fr) 2015-01-20 2017-11-28 Cytomx Therapeutics Inc Substrats clivables par métalloprotéase matricielle et clivables par sérine protéase et procédés d'utilisation de ceux-ci
CA2981098A1 (en) * 2015-03-31 2016-10-06 Vhsquared Limited Peptide construct having a protease-cleavable linker
US12049505B2 (en) 2018-12-06 2024-07-30 Cytomx Therapeutics, Inc. Matrix metalloprotease-cleavable and serine or cysteine protease-cleavable substrates and methods of use thereof
US20220228172A1 (en) * 2019-06-21 2022-07-21 Boehringer Ingelheim International Gmbh Novel Mechanism to Control RNA Virus Replication and Gene Expression

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP0368684B2 (de) 1988-11-11 2004-09-29 Medical Research Council Klonierung von Immunglobulin sequenzen aus den variabelen Domänen.
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
JPH06508511A (ja) * 1990-07-10 1994-09-29 ケンブリッジ　アンティボディー　テクノロジー　リミティド 特異的な結合ペアーの構成員の製造方法
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
DK1279731T3 (da) * 1991-03-01 2007-09-24 Dyax Corp Fremgangsmåde til udvikling af bindende miniproteiner
DE4122599C2 (de) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
ES2313867T3 (es) 1991-12-02 2009-03-16 Medical Research Council Produccion de anticuerpos anti-auto de repertorios de segmentos de anticuerpo expresados en la superficie de fagos.

Also Published As

Publication number Publication date
NO20005628D0 (no) 2000-11-08
EP1950295A1 (de) 2008-07-30
US7442159B1 (en) 2008-10-28
ATE380865T1 (de) 2007-12-15
GB0026143D0 (en) 2000-12-13
AU3941399A (en) 1999-11-29
CY1107293T1 (el) 2012-11-21
US20090203542A1 (en) 2009-08-13
WO1999058655A3 (en) 2000-01-27
EP1078051B1 (de) 2007-12-12
JP2010148507A (ja) 2010-07-08
ES2299244T3 (es) 2008-05-16
JP2002514413A (ja) 2002-05-21
NO329172B1 (no) 2010-09-06
AU762814B2 (en) 2003-07-03
PT1078051E (pt) 2008-03-17
EP1078051A2 (de) 2001-02-28
JP5161865B2 (ja) 2013-03-13
DE69937735T2 (de) 2008-11-27
GB2361237A (en) 2001-10-17
WO1999058655A2 (en) 1999-11-18
JP4493846B2 (ja) 2010-06-30
US8680018B2 (en) 2014-03-25
NO20005628L (no) 2001-01-12
CA2328422A1 (en) 1999-11-18
DK1078051T3 (da) 2008-04-07

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