PL2459702T3 - Pożywka do hodowli komórkowej do ekspresji białka adamts - Google Patents

Pożywka do hodowli komórkowej do ekspresji białka adamts

Info

Publication number
PL2459702T3
PL2459702T3 PL10740810.6T PL10740810T PL2459702T3 PL 2459702 T3 PL2459702 T3 PL 2459702T3 PL 10740810 T PL10740810 T PL 10740810T PL 2459702 T3 PL2459702 T3 PL 2459702T3
Authority
PL
Poland
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
PL10740810.6T
Other languages
English (en)
Polish (pl)
Inventor
Leopold Grillberger
Alexandra Spenger
Meinhard Hasslacher
Rana Grillberger
Manfred Reiter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of PL2459702T3 publication Critical patent/PL2459702T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
PL10740810.6T 2009-07-31 2010-07-30 Pożywka do hodowli komórkowej do ekspresji białka adamts PL2459702T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31

Publications (1)

Publication Number Publication Date
PL2459702T3 true PL2459702T3 (pl) 2016-12-30

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
PL10740810.6T PL2459702T3 (pl) 2009-07-31 2010-07-30 Pożywka do hodowli komórkowej do ekspresji białka adamts

Country Status (21)

Country Link
US (7) US8313926B2 (env)
EP (1) EP2459702B1 (env)
JP (5) JP5451884B2 (env)
KR (5) KR101574056B1 (env)
CN (2) CN102549145B (env)
AU (1) AU2010278751B2 (env)
BR (2) BR112012002151B1 (env)
CA (1) CA2769361A1 (env)
CO (1) CO6501191A2 (env)
EA (1) EA023193B1 (env)
ES (1) ES2583258T3 (env)
HR (1) HRP20160783T1 (env)
HU (1) HUE029110T2 (env)
IN (1) IN2012DN00801A (env)
MX (2) MX2012001261A (env)
NZ (1) NZ597727A (env)
PL (1) PL2459702T3 (env)
PT (1) PT2459702T (env)
SG (1) SG178194A1 (env)
SI (1) SI2459702T1 (env)
WO (1) WO2011014838A1 (env)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101574056B1 (ko) * 2009-07-31 2015-12-02 백스터 인터내셔널 인코포레이티드 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
PT3626736T (pt) 2010-07-08 2025-08-11 Takeda Pharmaceuticals Co Método de produção de fvw recombinante de elevado peso molecular na cultura celular
KR101662412B1 (ko) 2011-07-01 2016-10-04 암젠 인크 포유동물 세포 배양
JP2015531244A (ja) 2012-10-15 2015-11-02 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ タンパク質を製造するための哺乳動物細胞培養方法
EP2970916B1 (en) 2013-03-13 2021-04-14 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
KR102418824B1 (ko) * 2013-10-14 2022-07-11 아레스 트레이딩 에스.아. 포유동물의 고성능 유가식 배양을 위한 신규 배지
SG10202113019XA (en) 2014-06-04 2021-12-30 Amgen Inc Methods for harvesting mammalian cell cultures
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
EP3298138B1 (en) * 2015-05-22 2022-08-10 DuPont Nutrition Biosciences ApS Acetolactate decarboxylase
TWI870789B (zh) 2015-08-04 2025-01-21 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
EA201990373A1 (ru) 2016-08-04 2019-07-31 Баксалта Инкорпорэйтед Применение adamts13 для лечения, уменьшения интенсивности и/или предупреждения вазоокклюзивного криза при серповидноклеточной анемии, остром повреждении легкого и/или остром респираторном дистресс-синдроме
CN110168081B (zh) * 2016-11-04 2024-08-16 武田药品工业株式会社 腺相关病毒纯化方法
BR112020000127A2 (pt) * 2017-07-06 2020-07-07 Regeneron Pharmaceuticals, Inc. método para selecionar um hidrolisado de soja, e, glicoproteína
CA3094076C (en) * 2018-03-16 2025-11-25 Genzyme Corporation Methods for improving cell viability in a production bioreactor
US20210189358A1 (en) * 2018-06-18 2021-06-24 Synthetic Biologics, Inc. Methods of making alkaline phosphatase agents
EP4037704B1 (en) 2019-10-04 2025-12-03 The Children's Medical Center Corporation Adamts13 treatment to enhance graft survival
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
KR20240007293A (ko) 2019-12-06 2024-01-16 리제너론 파아마슈티컬스, 인크. 항-vegf 단백질 조성물 및 이를 생산하는 방법
AU2021248679A1 (en) 2020-04-02 2022-10-27 Takeda Pharmaceutical Company Limited ADAMTS13 variant, compositions, and uses thereof
MX2022013812A (es) 2020-05-08 2022-12-15 Regeneron Pharma Trampas y mini-trampas de vegf y metodos para el tratamiento de trastornos oculares y cancer.
EP4153215A1 (en) 2020-05-22 2023-03-29 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
WO2021246557A1 (ko) * 2020-06-05 2021-12-09 주식회사 프로앱텍 특정한 갯수의 알부민이 연결된 요산산화효소-알부민 복합체 및 이의 제조 방법
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
ATE542891T1 (de) 1994-11-10 2012-02-15 Baxter Healthcare Sa Verfahren zur herstellung von biologischen produkten in protein-freier kultur
WO1996026266A1 (en) 1995-02-23 1996-08-29 Quest International B.V. Peptides for tissue and cell culture media
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
IT1285158B1 (it) 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
DE69717650T2 (de) 1996-10-07 2003-09-18 Solutia Inc., St. Louis Verfahren zur hydroxylierung von benzol
JP4543402B2 (ja) 1996-10-10 2010-09-15 ライフ テクノロジーズ コーポレーション 植物由来栄養素を含む動物細胞培養培地
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
US7670839B2 (en) 2002-12-23 2010-03-02 Fuji Film Manufacturing Europe B.V. Process for coating cell-culture support
AU2005248299A1 (en) * 2004-04-16 2005-12-08 Wyeth ADAMTS-8 proteins and uses thereof
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Ａｄａｍｔｓ１３の安定化方法
SI2522717T1 (sl) * 2006-01-04 2014-05-30 Baxter International Inc. Mediji celične kulture brez oligopeptidov
CN101611268B (zh) * 2006-12-22 2013-06-12 斯普灵格开利有限公司 用于空调单元的新鲜空气控制杆
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
CA2709890A1 (en) 2007-12-27 2009-07-09 Andre Giovagnoli Cell culture processes
KR101574056B1 (ko) * 2009-07-31 2015-12-02 백스터 인터내셔널 인코포레이티드 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지

Also Published As

Publication number Publication date
JP5451884B2 (ja) 2014-03-26
CO6501191A2 (es) 2012-08-15
CN104962539B (zh) 2019-09-17
EP2459702A1 (en) 2012-06-06
KR20170140411A (ko) 2017-12-20
US9127265B2 (en) 2015-09-08
KR101808496B1 (ko) 2017-12-12
JP2016144471A (ja) 2016-08-12
KR101756354B1 (ko) 2017-07-26
US20170051270A1 (en) 2017-02-23
CA2769361A1 (en) 2011-02-03
JP2021112210A (ja) 2021-08-05
US8759026B2 (en) 2014-06-24
JP2014061005A (ja) 2014-04-10
US20110086413A1 (en) 2011-04-14
CN102549145A (zh) 2012-07-04
JP2013500724A (ja) 2013-01-10
EA023193B1 (ru) 2016-05-31
HUE029110T2 (en) 2017-02-28
US9441216B2 (en) 2016-09-13
AU2010278751B2 (en) 2014-05-15
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
JP2018093881A (ja) 2018-06-21
US20190071660A1 (en) 2019-03-07
US20130071906A1 (en) 2013-03-21
JP7083059B2 (ja) 2022-06-09
IN2012DN00801A (env) 2015-06-26
KR20150044981A (ko) 2015-04-27
US11254921B2 (en) 2022-02-22
JP6883071B2 (ja) 2021-06-09
MX344970B (es) 2017-01-12
US20200308566A1 (en) 2020-10-01
KR20170081747A (ko) 2017-07-12
KR20120046301A (ko) 2012-05-09
BR112012002151B1 (pt) 2021-07-06
BR122021005965B1 (pt) 2022-01-25
BR112012002151A2 (pt) 2020-03-03
JP2019201661A (ja) 2019-11-28
CN104962539A (zh) 2015-10-07
AU2010278751A1 (en) 2012-02-09
JP6511007B2 (ja) 2019-05-08
ES2583258T3 (es) 2016-09-20
KR20150138425A (ko) 2015-12-09
WO2011014838A1 (en) 2011-02-03
JP6567707B2 (ja) 2019-08-28
JP5936232B2 (ja) 2016-06-22
SG178194A1 (en) 2012-03-29
US20150329848A1 (en) 2015-11-19
HK1171790A1 (zh) 2013-04-05
CN102549145B (zh) 2015-05-20
KR101574056B1 (ko) 2015-12-02
HRP20160783T1 (hr) 2016-08-12
US8313926B2 (en) 2012-11-20
US20140329294A1 (en) 2014-11-06
US10072254B2 (en) 2018-09-11
EA201270224A1 (ru) 2012-09-28
PT2459702T (pt) 2016-08-02
EP2459702B1 (en) 2016-05-04
KR101573972B1 (ko) 2015-12-02
MX2012001261A (es) 2012-05-22
NZ597727A (en) 2013-08-30
KR101912552B1 (ko) 2018-10-26

Similar Documents

Publication Publication Date Title
PT2459702T (pt) Meio de cultura de células para a expressão da proteína adamts
IL222452A0 (en) Improved cell culture medium
IL226971B (en) Cell culture medium
EP2398896A4 (en) CELL CULTURE MEDIUM CONTAINING COMBINATIONS OF PROTEINS
SG10201503196VA (en) Cell Culture Medium Comprising Small Peptides
PL2393917T3 (pl) Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających komórki macierzyste
GB2458863B (en) Improved culture of stem cells
IL222453A0 (en) Improved cell culture medium
PL2366021T3 (pl) Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach
GB0613756D0 (en) Cell culture medium
ZA201000715B (en) Single pluripotent stem cell culture
GB0914195D0 (en) Vessel for culturing cells
GB2460552B (en) Stem cell culture media and methods
EP2511364A4 (en) METHOD FOR CULTURING CELLS OR MICRO-ORGANISMS
GB0800524D0 (en) Cell culture system
GB0817990D0 (en) Cell culture vessel
IL215602A0 (en) Method for the cultivation of cell population in a serum free medium
PL2480655T3 (pl) Podłoże do hodowli komórkowych
GB0808778D0 (en) Cell culture technique
GB0922428D0 (en) Cell culture
GB0915268D0 (en) Cell culture
AU2009901852A0 (en) Media and methods for cell culture
HUE050984T2 (hu) Tenyészközeg epiteliális õssejtekhez és az õssejteket tartalmazó organoidokhoz
PT3061808T (pt) Meio de cultura para células estaminais epiteliais e organóides compreeendendo as referidas células estaminais
GB0901069D0 (en) Stem cell culture methods