NO333769B1 - Genetisk modifisert gjaerstamme som autonomt produserer steroider, fremgangsmate for fremstilling samt farmasoytisk preparat. - Google Patents
Genetisk modifisert gjaerstamme som autonomt produserer steroider, fremgangsmate for fremstilling samt farmasoytisk preparat. Download PDFInfo
- Publication number
- NO333769B1 NO333769B1 NO20033242A NO20033242A NO333769B1 NO 333769 B1 NO333769 B1 NO 333769B1 NO 20033242 A NO20033242 A NO 20033242A NO 20033242 A NO20033242 A NO 20033242A NO 333769 B1 NO333769 B1 NO 333769B1
- Authority
- NO
- Norway
- Prior art keywords
- plasmid
- yeast
- gene
- yeast strain
- strain according
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 165
- 150000003431 steroids Chemical class 0.000 title claims abstract description 95
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 35
- 239000000825 pharmaceutical preparation Substances 0.000 title claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 26
- 239000013612 plasmid Substances 0.000 claims description 306
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 160
- 108090000623 proteins and genes Proteins 0.000 claims description 139
- 230000014509 gene expression Effects 0.000 claims description 111
- 239000002299 complementary DNA Substances 0.000 claims description 70
- 102000004169 proteins and genes Human genes 0.000 claims description 70
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 58
- ORNBQBCIOKFOEO-YQUGOWONSA-N Pregnenolone Natural products O=C(C)[C@@H]1[C@@]2(C)[C@H]([C@H]3[C@@H]([C@]4(C)C(=CC3)C[C@@H](O)CC4)CC2)CC1 ORNBQBCIOKFOEO-YQUGOWONSA-N 0.000 claims description 32
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 32
- ORNBQBCIOKFOEO-QGVNFLHTSA-N pregnenolone Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 ORNBQBCIOKFOEO-QGVNFLHTSA-N 0.000 claims description 32
- 229960000249 pregnenolone Drugs 0.000 claims description 32
- 229960000890 hydrocortisone Drugs 0.000 claims description 29
- 101000579123 Homo sapiens Phosphoglycerate kinase 1 Proteins 0.000 claims description 27
- 239000000186 progesterone Substances 0.000 claims description 26
- 229960003387 progesterone Drugs 0.000 claims description 26
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 claims description 25
- KJWZYMMLVHIVSU-IYCNHOCDSA-N PGK1 Chemical compound CCCCC[C@H](O)\C=C\[C@@H]1[C@@H](CCCCCCC(O)=O)C(=O)CC1=O KJWZYMMLVHIVSU-IYCNHOCDSA-N 0.000 claims description 24
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- 101100480861 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) tdh gene Proteins 0.000 claims description 21
- 101100447466 Candida albicans (strain WO-1) TDH1 gene Proteins 0.000 claims description 21
- 101150088047 tdh3 gene Proteins 0.000 claims description 21
- 108090000854 Oxidoreductases Proteins 0.000 claims description 20
- 101100121588 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCY1 gene Proteins 0.000 claims description 19
- 210000003470 mitochondria Anatomy 0.000 claims description 18
- WHBHBVVOGNECLV-OBQKJFGGSA-N 11-deoxycortisol Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 WHBHBVVOGNECLV-OBQKJFGGSA-N 0.000 claims description 17
- 101000974934 Homo sapiens Cyclic AMP-dependent transcription factor ATF-2 Proteins 0.000 claims description 17
- 101000997829 Homo sapiens Glial cell line-derived neurotrophic factor Proteins 0.000 claims description 17
- 102000004316 Oxidoreductases Human genes 0.000 claims description 17
- 102100023033 Cyclic AMP-dependent transcription factor ATF-2 Human genes 0.000 claims description 16
- 108010046335 Ferredoxin-NADP Reductase Proteins 0.000 claims description 15
- 102100036777 NADPH:adrenodoxin oxidoreductase, mitochondrial Human genes 0.000 claims description 15
- 101150098080 ERG5 gene Proteins 0.000 claims description 14
- 101000780532 Homo sapiens ADP-ribosylhydrolase ARH1 Proteins 0.000 claims description 13
- 229930182558 Sterol Natural products 0.000 claims description 13
- 230000010354 integration Effects 0.000 claims description 13
- 230000010076 replication Effects 0.000 claims description 13
- 150000003432 sterols Chemical class 0.000 claims description 13
- 235000003702 sterols Nutrition 0.000 claims description 13
- 210000000349 chromosome Anatomy 0.000 claims description 12
- 230000002779 inactivation Effects 0.000 claims description 12
- 102000003804 Adrenodoxin Human genes 0.000 claims description 11
- 108090000187 Adrenodoxin Proteins 0.000 claims description 11
- 101100533888 Hypocrea jecorina (strain QM6a) sor4 gene Proteins 0.000 claims description 11
- 101100053441 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YPR1 gene Proteins 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 101150009006 HIS3 gene Proteins 0.000 claims description 10
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 claims description 10
- 210000004027 cell Anatomy 0.000 claims description 10
- 102100024637 Galectin-10 Human genes 0.000 claims description 9
- 101000749871 Mus musculus Contactin-associated protein 1 Proteins 0.000 claims description 9
- 239000002243 precursor Substances 0.000 claims description 9
- DBPWSSGDRRHUNT-CEGNMAFCSA-N 17α-hydroxyprogesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2 DBPWSSGDRRHUNT-CEGNMAFCSA-N 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- -1 LEU2 Proteins 0.000 claims description 6
- JERGUCIJOXJXHF-TVWVXWENSA-N 17alpha-hydroxypregnenolone Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2 JERGUCIJOXJXHF-TVWVXWENSA-N 0.000 claims description 5
- 101150069942 ATR2 gene Proteins 0.000 claims description 5
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims description 5
- 102100027341 Neutral and basic amino acid transport protein rBAT Human genes 0.000 claims description 5
- 108010011732 Steroid 21-Hydroxylase Proteins 0.000 claims description 5
- 102000014169 Steroid 21-Hydroxylase Human genes 0.000 claims description 5
- 230000002759 chromosomal effect Effects 0.000 claims description 5
- 210000000172 cytosol Anatomy 0.000 claims description 5
- 230000004060 metabolic process Effects 0.000 claims description 5
- 101150069620 ARE2 gene Proteins 0.000 claims description 4
- 101100459266 Arabidopsis thaliana MYC3 gene Proteins 0.000 claims description 4
- 101000981773 Arabidopsis thaliana Transcription factor MYB34 Proteins 0.000 claims description 4
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 claims description 4
- 101710088194 Dehydrogenase Proteins 0.000 claims description 4
- 101000651887 Homo sapiens Neutral and basic amino acid transport protein rBAT Proteins 0.000 claims description 4
- 101000955959 Homo sapiens Vacuolar protein sorting-associated protein 52 homolog Proteins 0.000 claims description 4
- 108090000769 Isomerases Proteins 0.000 claims description 4
- 102000004195 Isomerases Human genes 0.000 claims description 4
- 101100194350 Mus musculus Rere gene Proteins 0.000 claims description 4
- 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims description 4
- 102100038937 Vacuolar protein sorting-associated protein 52 homolog Human genes 0.000 claims description 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 4
- 108010080691 Alcohol O-acetyltransferase Proteins 0.000 claims description 3
- 108010084976 Cholesterol Side-Chain Cleavage Enzyme Proteins 0.000 claims description 3
- 102100027516 Cholesterol side-chain cleavage enzyme, mitochondrial Human genes 0.000 claims description 3
- 108010007167 Cytochromes b5 Proteins 0.000 claims description 3
- 101000861327 Homo sapiens Cholesterol side-chain cleavage enzyme, mitochondrial Proteins 0.000 claims description 3
- 210000002230 centromere Anatomy 0.000 claims description 3
- 101001011019 Gallus gallus Gallinacin-10 Proteins 0.000 claims description 2
- 101001011021 Gallus gallus Gallinacin-12 Proteins 0.000 claims description 2
- 208000020584 Polyploidy Diseases 0.000 claims description 2
- 101150044379 TIR1 gene Proteins 0.000 claims description 2
- 230000003322 aneuploid effect Effects 0.000 claims description 2
- 208000036878 aneuploidy Diseases 0.000 claims description 2
- 102000003849 Cytochrome P450 Human genes 0.000 claims 2
- 102100023026 Cyclic AMP-dependent transcription factor ATF-1 Human genes 0.000 claims 1
- 102000007605 Cytochromes b5 Human genes 0.000 claims 1
- 101100010928 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) tuf gene Proteins 0.000 claims 1
- 102100021719 Steroid 17-alpha-hydroxylase/17,20 lyase Human genes 0.000 claims 1
- 101710163849 Steroid 17-alpha-hydroxylase/17,20 lyase Proteins 0.000 claims 1
- 101150001810 TEAD1 gene Proteins 0.000 claims 1
- 101150074253 TEF1 gene Proteins 0.000 claims 1
- 102100029898 Transcriptional enhancer factor TEF-1 Human genes 0.000 claims 1
- 239000012634 fragment Substances 0.000 description 67
- 101150050575 URA3 gene Proteins 0.000 description 41
- 230000000694 effects Effects 0.000 description 41
- 238000010276 construction Methods 0.000 description 36
- 239000002609 medium Substances 0.000 description 30
- 108091034117 Oligonucleotide Proteins 0.000 description 28
- 101710118918 Transcription elongation factor 1 Proteins 0.000 description 27
- 101150003525 atf-2 gene Proteins 0.000 description 27
- 241000283690 Bos taurus Species 0.000 description 25
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 25
- 229960000988 nystatin Drugs 0.000 description 21
- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 21
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 20
- 239000000047 product Substances 0.000 description 19
- 239000003550 marker Substances 0.000 description 18
- 102000004190 Enzymes Human genes 0.000 description 17
- 108090000790 Enzymes Proteins 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 16
- 230000009466 transformation Effects 0.000 description 16
- 108091026890 Coding region Proteins 0.000 description 15
- 101150093622 GCY1 gene Proteins 0.000 description 14
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 14
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 14
- 230000015572 biosynthetic process Effects 0.000 description 13
- 239000000758 substrate Substances 0.000 description 13
- 102100034119 ADP-ribosylhydrolase ARH1 Human genes 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 12
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 12
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- WHBHBVVOGNECLV-UHFFFAOYSA-N 11-deoxy-17-hydroxy-corticosterone Natural products O=C1CCC2(C)C3CCC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 WHBHBVVOGNECLV-UHFFFAOYSA-N 0.000 description 11
- 235000012000 cholesterol Nutrition 0.000 description 11
- 102000018832 Cytochromes Human genes 0.000 description 10
- 108010052832 Cytochromes Proteins 0.000 description 10
- 230000006798 recombination Effects 0.000 description 10
- 238000005215 recombination Methods 0.000 description 10
- 238000003786 synthesis reaction Methods 0.000 description 10
- 229940035893 uracil Drugs 0.000 description 10
- 101150084750 1 gene Proteins 0.000 description 9
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 9
- 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 9
- 101150108183 ARH1 gene Proteins 0.000 description 9
- 108020004635 Complementary DNA Proteins 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 9
- 101100434456 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ARH1 gene Proteins 0.000 description 9
- 239000011159 matrix material Substances 0.000 description 9
- SGNBVLSWZMBQTH-FGAXOLDCSA-N Campesterol Natural products O[C@@H]1CC=2[C@@](C)([C@@H]3[C@H]([C@H]4[C@@](C)([C@H]([C@H](CC[C@H](C(C)C)C)C)CC4)CC3)CC=2)CC1 SGNBVLSWZMBQTH-FGAXOLDCSA-N 0.000 description 8
- BTEISVKTSQLKST-UHFFFAOYSA-N Haliclonasterol Natural products CC(C=CC(C)C(C)(C)C)C1CCC2C3=CC=C4CC(O)CCC4(C)C3CCC12C BTEISVKTSQLKST-UHFFFAOYSA-N 0.000 description 8
- 101150102471 YPR1 gene Proteins 0.000 description 8
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 8
- SGNBVLSWZMBQTH-PODYLUTMSA-N campesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](C)C(C)C)[C@@]1(C)CC2 SGNBVLSWZMBQTH-PODYLUTMSA-N 0.000 description 8
- 235000000431 campesterol Nutrition 0.000 description 8
- 229930182817 methionine Natural products 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 101150096273 ADE2 gene Proteins 0.000 description 7
- 229930024421 Adenine Natural products 0.000 description 7
- 102100032566 Carbonic anhydrase-related protein 10 Human genes 0.000 description 7
- 101150038242 GAL10 gene Proteins 0.000 description 7
- 101000867836 Homo sapiens Carbonic anhydrase-related protein 10 Proteins 0.000 description 7
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 7
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 7
- CRRKVZVYZQXICQ-RJJCNJEVSA-N Pregnenolone acetate Chemical compound C([C@@H]12)C[C@]3(C)[C@@H](C(C)=O)CC[C@H]3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)C)C1 CRRKVZVYZQXICQ-RJJCNJEVSA-N 0.000 description 7
- 229960000643 adenine Drugs 0.000 description 7
- 230000003321 amplification Effects 0.000 description 7
- 230000001965 increasing effect Effects 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 230000003071 parasitic effect Effects 0.000 description 7
- 239000007320 rich medium Substances 0.000 description 7
- 230000002103 transcriptional effect Effects 0.000 description 7
- 101100084595 Caenorhabditis elegans pam-1 gene Proteins 0.000 description 6
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 238000006640 acetylation reaction Methods 0.000 description 6
- 102000005421 acetyltransferase Human genes 0.000 description 6
- 108020002494 acetyltransferase Proteins 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 239000013613 expression plasmid Substances 0.000 description 6
- 238000004817 gas chromatography Methods 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 230000002018 overexpression Effects 0.000 description 6
- 230000037361 pathway Effects 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 101100269301 Bos taurus FDX1 gene Proteins 0.000 description 5
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 description 5
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 5
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000012239 gene modification Methods 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 238000013518 transcription Methods 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 229930182830 galactose Natural products 0.000 description 4
- 230000005017 genetic modification Effects 0.000 description 4
- 235000013617 genetically modified food Nutrition 0.000 description 4
- 230000033444 hydroxylation Effects 0.000 description 4
- 238000005805 hydroxylation reaction Methods 0.000 description 4
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 230000037353 metabolic pathway Effects 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000000844 transformation Methods 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 3
- 102000009878 3-Hydroxysteroid Dehydrogenases Human genes 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 102100024630 Asc-type amino acid transporter 1 Human genes 0.000 description 3
- VLWMWRUVVUBMRS-GDFOZHQMSA-N C1C=C2CCCC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)C=C[C@H](C)C(CO)C)[C@@]1(C)CC2 Chemical compound C1C=C2CCCC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)C=C[C@H](C)C(CO)C)[C@@]1(C)CC2 VLWMWRUVVUBMRS-GDFOZHQMSA-N 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 108010000521 Human Growth Hormone Proteins 0.000 description 3
- 108091029795 Intergenic region Proteins 0.000 description 3
- 101150007280 LEU2 gene Proteins 0.000 description 3
- 108090000856 Lyases Proteins 0.000 description 3
- 102000004317 Lyases Human genes 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 101150053185 P450 gene Proteins 0.000 description 3
- 101000744001 Ruminococcus gnavus (strain ATCC 29149 / VPI C7-9) 3beta-hydroxysteroid dehydrogenase Proteins 0.000 description 3
- 101150081875 Slc7a10 gene Proteins 0.000 description 3
- 230000021736 acetylation Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 150000001841 cholesterols Chemical class 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 230000002438 mitochondrial effect Effects 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000028070 sporulation Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 2
- SEHFUALWMUWDKS-UHFFFAOYSA-N 5-fluoroorotic acid Chemical compound OC(=O)C=1NC(=O)NC(=O)C=1F SEHFUALWMUWDKS-UHFFFAOYSA-N 0.000 description 2
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 108010084469 Aldo-Keto Reductases Proteins 0.000 description 2
- 102000005602 Aldo-Keto Reductases Human genes 0.000 description 2
- 101100519158 Arabidopsis thaliana PCR2 gene Proteins 0.000 description 2
- 101100519159 Arabidopsis thaliana PCR3 gene Proteins 0.000 description 2
- 101100519161 Arabidopsis thaliana PCR5 gene Proteins 0.000 description 2
- 101100519163 Arabidopsis thaliana PCR7 gene Proteins 0.000 description 2
- 101100519164 Arabidopsis thaliana PCR8 gene Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101000761630 Bos taurus Steroid Delta-isomerase Proteins 0.000 description 2
- OILXMJHPFNGGTO-NRHJOKMGSA-N Brassicasterol Natural products O[C@@H]1CC=2[C@@](C)([C@@H]3[C@H]([C@H]4[C@](C)([C@H]([C@@H](/C=C/[C@H](C(C)C)C)C)CC4)CC3)CC=2)CC1 OILXMJHPFNGGTO-NRHJOKMGSA-N 0.000 description 2
- 101100026251 Caenorhabditis elegans atf-2 gene Proteins 0.000 description 2
- OMFXVFTZEKFJBZ-UHFFFAOYSA-N Corticosterone Natural products O=C1CCC2(C)C3C(O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 OMFXVFTZEKFJBZ-UHFFFAOYSA-N 0.000 description 2
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 2
- 102100031655 Cytochrome b5 Human genes 0.000 description 2
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 2
- 101150037782 GAL2 gene Proteins 0.000 description 2
- 102100021735 Galectin-2 Human genes 0.000 description 2
- 102100033295 Glial cell line-derived neurotrophic factor Human genes 0.000 description 2
- 101000701405 Homo sapiens Serine/threonine-protein kinase 36 Proteins 0.000 description 2
- 101000861263 Homo sapiens Steroid 21-hydroxylase Proteins 0.000 description 2
- 101100062121 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cyc-1 gene Proteins 0.000 description 2
- 101150102573 PCR1 gene Proteins 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- OILXMJHPFNGGTO-ZRUUVFCLSA-N UNPD197407 Natural products C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)C=C[C@H](C)C(C)C)[C@@]1(C)CC2 OILXMJHPFNGGTO-ZRUUVFCLSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
- OILXMJHPFNGGTO-ZAUYPBDWSA-N brassicasterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)/C=C/[C@H](C)C(C)C)[C@@]1(C)CC2 OILXMJHPFNGGTO-ZAUYPBDWSA-N 0.000 description 2
- 235000004420 brassicasterol Nutrition 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000005515 coenzyme Substances 0.000 description 2
- OMFXVFTZEKFJBZ-HJTSIMOOSA-N corticosterone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@H](CC4)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OMFXVFTZEKFJBZ-HJTSIMOOSA-N 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 101150050623 erg-6 gene Proteins 0.000 description 2
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 229960002885 histidine Drugs 0.000 description 2
- 102000053342 human STK36 Human genes 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 235000011056 potassium acetate Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 108010065742 rat 3 beta-hydroxy-delta 5-steroid dehydrogenase Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- NLQLSVXGSXCXFE-UHFFFAOYSA-N sitosterol Natural products CC=C(/CCC(C)C1CC2C3=CCC4C(C)C(O)CCC4(C)C3CCC2(C)C1)C(C)C NLQLSVXGSXCXFE-UHFFFAOYSA-N 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- 229960004799 tryptophan Drugs 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- ISBSSBGEYIBVTO-TYKWNDPBSA-N (20R,22R)-20,22-dihydroxycholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@@](C)(O)[C@H](O)CCC(C)C)[C@@]1(C)CC2 ISBSSBGEYIBVTO-TYKWNDPBSA-N 0.000 description 1
- FUFLCEKSBBHCMO-UHFFFAOYSA-N 11-dehydrocorticosterone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 FUFLCEKSBBHCMO-UHFFFAOYSA-N 0.000 description 1
- MASCESDECGBIBB-JAKCRWNRSA-N 17,20-dihydroxypregn-4-en-3-one Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(O)C)(O)[C@@]1(C)CC2 MASCESDECGBIBB-JAKCRWNRSA-N 0.000 description 1
- DBPWSSGDRRHUNT-UHFFFAOYSA-N 17alpha-hydroxy progesterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(=O)C)(O)C1(C)CC2 DBPWSSGDRRHUNT-UHFFFAOYSA-N 0.000 description 1
- 108091006112 ATPases Proteins 0.000 description 1
- 101150091866 ATR12 gene Proteins 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241001244729 Apalis Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100163849 Arabidopsis thaliana ARS1 gene Proteins 0.000 description 1
- 101100519160 Arabidopsis thaliana PCR4 gene Proteins 0.000 description 1
- 101100519162 Arabidopsis thaliana PCR6 gene Proteins 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 101000928262 Bos taurus NADPH:adrenodoxin oxidoreductase, mitochondrial Proteins 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 101100497948 Caenorhabditis elegans cyn-1 gene Proteins 0.000 description 1
- 101100448223 Caenorhabditis elegans gcy-1 gene Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 108010036781 Fumarate Hydratase Proteins 0.000 description 1
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 101710162684 Glyceraldehyde-3-phosphate dehydrogenase 3 Proteins 0.000 description 1
- 101150091750 HMG1 gene Proteins 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101001017818 Homo sapiens ATP-dependent translocase ABCB1 Proteins 0.000 description 1
- 101001023837 Homo sapiens Cysteine desulfurase, mitochondrial Proteins 0.000 description 1
- 101001077418 Homo sapiens Potassium voltage-gated channel subfamily H member 6 Proteins 0.000 description 1
- 241001138401 Kluyveromyces lactis Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101150090765 NCP1 gene Proteins 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 101100390566 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) dnr-8 gene Proteins 0.000 description 1
- 101150080712 PDR12 gene Proteins 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 101150096292 Ppme1 gene Proteins 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 101150089878 RAD2 gene Proteins 0.000 description 1
- 108091007187 Reductases Proteins 0.000 description 1
- 101100497944 Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) cyp11 gene Proteins 0.000 description 1
- 101100459905 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NCP1 gene Proteins 0.000 description 1
- 101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108010015330 Steroid 17-alpha-Hydroxylase Proteins 0.000 description 1
- 102000001854 Steroid 17-alpha-Hydroxylase Human genes 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101150015037 TGL1 gene Proteins 0.000 description 1
- 101150006914 TRP1 gene Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- PBCJIPOGFJYBJE-UHFFFAOYSA-N acetonitrile;hydrate Chemical compound O.CC#N PBCJIPOGFJYBJE-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 210000004100 adrenal gland Anatomy 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- AEMFNILZOJDQLW-QAGGRKNESA-N androst-4-ene-3,17-dione Chemical class O=C1CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 AEMFNILZOJDQLW-QAGGRKNESA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000002989 correction material Substances 0.000 description 1
- 150000001886 cortisols Chemical class 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
- 101150046305 cpr-1 gene Proteins 0.000 description 1
- 238000007257 deesterification reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000008686 ergosterol biosynthesis Effects 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 101150035621 fen1 gene Proteins 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 229960002899 hydroxyprogesterone Drugs 0.000 description 1
- 229950000801 hydroxyprogesterone caproate Drugs 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 150000002496 iodine Chemical class 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 229960003136 leucine Drugs 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229940037128 systemic glucocorticoids Drugs 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 108010082737 zymolyase Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
- C12Y114/15004—Steroid 11-beta-monooxygenase (1.14.15.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/005—Degradation of the lateral chains at position 17
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/06—Hydroxylating
- C12P33/08—Hydroxylating at 11 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/12—Acting on D ring
- C12P33/16—Acting at 17 position
- C12P33/18—Hydroxylating at 17 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
- C12Y114/15006—Cholesterol monooxygenase (side-chain-cleaving) (1.14.15.6), i.e. cytochrome P450scc
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/99—Miscellaneous (1.14.99)
- C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/99—Miscellaneous (1.14.99)
- C12Y114/9901—Steroid 21-monooxygenase (1.14.99.10), i.e. steroid cytochrome P450 21-hydroxylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
- C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
- C12Y118/01002—Ferredoxin-NADP+ reductase (1.18.1.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Vascular Medicine (AREA)
- Transplantation (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Psychology (AREA)
- Urology & Nephrology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0101294A FR2820145B1 (fr) | 2001-01-31 | 2001-01-31 | Souche de levure produisant des steroides de facon autonome |
PCT/FR2002/000348 WO2002061109A2 (fr) | 2001-01-31 | 2002-01-29 | Souches de levures produisant des steroides de facon autonome |
Publications (3)
Publication Number | Publication Date |
---|---|
NO20033242D0 NO20033242D0 (no) | 2003-07-17 |
NO20033242L NO20033242L (no) | 2003-09-04 |
NO333769B1 true NO333769B1 (no) | 2013-09-16 |
Family
ID=8859443
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20033242A NO333769B1 (no) | 2001-01-31 | 2003-07-17 | Genetisk modifisert gjaerstamme som autonomt produserer steroider, fremgangsmate for fremstilling samt farmasoytisk preparat. |
Country Status (20)
Country | Link |
---|---|
US (2) | US7670829B2 (el) |
EP (1) | EP1385980B1 (el) |
JP (1) | JP4620932B2 (el) |
KR (1) | KR100882150B1 (el) |
AR (1) | AR033857A1 (el) |
AT (1) | ATE486134T1 (el) |
BR (1) | BRPI0206809B8 (el) |
CA (1) | CA2435096C (el) |
CY (1) | CY1111099T1 (el) |
DE (1) | DE60238114D1 (el) |
DK (1) | DK1385980T3 (el) |
ES (1) | ES2354603T3 (el) |
FR (1) | FR2820145B1 (el) |
IL (2) | IL157118A0 (el) |
MX (1) | MXPA03006031A (el) |
NO (1) | NO333769B1 (el) |
NZ (1) | NZ526381A (el) |
PT (1) | PT1385980E (el) |
WO (1) | WO2002061109A2 (el) |
ZA (1) | ZA200305772B (el) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2812884B1 (fr) | 2000-08-08 | 2002-11-08 | Aventis Pharma Sa | Levures modifiees et utilisations, notamment pour la production de derives steroidiens |
US20060177416A1 (en) | 2003-10-14 | 2006-08-10 | Medivas, Llc | Polymer particle delivery compositions and methods of use |
FR2820145B1 (fr) | 2001-01-31 | 2004-01-23 | Aventis Pharma Sa | Souche de levure produisant des steroides de facon autonome |
US20070160622A1 (en) * | 2005-12-07 | 2007-07-12 | Medivas, Llc | Method for assembling a polymer-biologic delivery composition |
FR2869914B1 (fr) * | 2004-05-06 | 2012-11-09 | Aventis Pharma Sa | Souches de levure produisant du cholesterol et leurs applications |
GB0512707D0 (en) * | 2005-06-22 | 2005-07-27 | Delta Biotechnology Ltd | Gene expression technique |
EP1926780B1 (en) | 2005-09-22 | 2013-08-14 | Medivas, LLC | Bis-( -amino)-diol-diester-containing poly(ester amide) and poly(ester urethane) compositions and methods of use |
EP1933881B1 (en) * | 2005-09-22 | 2019-03-13 | Medivas, LLC | Solid polymer delivery compositions and methods for use thereof |
JP5196498B2 (ja) * | 2006-05-09 | 2013-05-15 | メディバス エルエルシー | 生分解性水溶性ポリマー |
EP2178944A1 (en) * | 2007-07-24 | 2010-04-28 | Medivas, LLC | Biodegradable cationic polymer gene transfer compositions and methods of use |
CA2733686A1 (en) * | 2008-08-13 | 2010-02-18 | Medivas, Llc | Aabb-poly(depsipeptide) biodegradable polymers and methods of use |
JPWO2010079594A1 (ja) | 2009-01-07 | 2012-06-21 | 三菱化学株式会社 | ステロール側鎖切断酵素蛋白質およびその利用 |
FR2961218A1 (fr) | 2010-06-10 | 2011-12-16 | Sanofi Aventis | Methode de detection de composes modulateurs du metabolisme du cholesterol |
KR200458070Y1 (ko) * | 2010-06-21 | 2012-01-19 | 주광진 | 양치 기능이 부가된 젖꼭지 |
WO2012095816A1 (en) | 2011-01-14 | 2012-07-19 | Sanofi | Method for detecting the biological activity of alpha-synuclein |
FR2976949B1 (fr) * | 2011-06-21 | 2015-08-28 | Sanofi Sa | Procede de preparation de levures genetiquement transformees capables de produire une molecule d'interet a haut titre |
US9873765B2 (en) | 2011-06-23 | 2018-01-23 | Dsm Ip Assets, B.V. | Biodegradable polyesteramide copolymers for drug delivery |
JP6045575B2 (ja) | 2011-06-23 | 2016-12-14 | ディーエスエム アイピー アセッツ ビー.ブイ. | 薬物送達のための新規な生分解性ポリエステルアミドコポリマー |
AU2014283301A1 (en) * | 2013-06-17 | 2016-01-07 | Sanofi | Whole-cell system for cytochrome P450 monooxygenases biocatalysis |
JP6720447B2 (ja) | 2014-12-18 | 2020-07-08 | ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. | 酸感受性薬剤の送達のための薬剤送達系 |
EP3130680A1 (en) * | 2015-08-11 | 2017-02-15 | Universitat de Girona | Method for the detection, follow up and/or classification of intestinal diseases |
US11693017B2 (en) | 2015-09-18 | 2023-07-04 | President And Fellows Of Harvard College | Small molecule biosensors |
CN108866142B (zh) * | 2017-05-10 | 2022-08-16 | 中国科学院分子植物科学卓越创新中心 | 细胞色素p450、烟酰胺腺嘌呤二核苷酸-细胞色素p450还原酶及其应用 |
CN110903993A (zh) * | 2019-12-20 | 2020-03-24 | 河北兰升生物科技有限公司 | 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用 |
CN114456964B (zh) * | 2022-03-15 | 2023-02-03 | 陕西海斯夫生物工程有限公司 | 一种高产豆甾醇的重组解脂亚罗酵母、其构建方法、用于产豆甾醇的发酵培养基及应用 |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6447380A (en) * | 1987-08-19 | 1989-02-21 | Agency Ind Science Techn | Steroid-oxidizing yeast strain |
IL90207A (en) * | 1988-05-06 | 1994-07-31 | Roussel Uclaf | Biochemical oxidation of steroids and genetically engineered cells to be used therefor |
EP0340878B1 (en) | 1988-05-06 | 2001-05-16 | Aventis Pharma S.A. | Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor |
JPH0231680A (ja) | 1988-07-22 | 1990-02-01 | Agency Of Ind Science & Technol | チトクロムP450c↓2↓1産生酵母菌株 |
CN1042567A (zh) * | 1988-09-23 | 1990-05-30 | 吉斯特-布罗卡迪斯公司 | 类固醇多步氧化方法和所用的遗传工程细胞 |
JPH02249488A (ja) | 1989-03-22 | 1990-10-05 | Sumitomo Chem Co Ltd | チトクロムP450↓c↓2↓1と酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素、該酵素をコードする遺伝子および該酵素の製造方法 |
JP2645130B2 (ja) | 1989-03-31 | 1997-08-25 | 日清製粉株式会社 | ステロイド誘導体 |
FR2678611B1 (fr) | 1991-07-04 | 1995-01-20 | Roussel Uclaf | Nouveaux esters pyrethrinouides de l'alcool 1,3,4,5,6,7-hexahydro 1,3-dioxo-2h-isoindol-2-yl-methylique, leur procede de preparation et leur application comme pesticides. |
JP3363197B2 (ja) | 1992-06-18 | 2003-01-08 | 麒麟麦酒株式会社 | アルコールアセチルトランスフェラーゼ遺伝子およびその利用 |
US5547868A (en) | 1993-06-09 | 1996-08-20 | Regents Of The University Of California | Cholesterol disposal fusion enzymes |
IL116872A (en) | 1995-02-15 | 2004-12-15 | Aventis Pharma Sa | Delta 5,7-Strol Reductase from Arbidopsis Thaliana |
DE19505578A1 (de) | 1995-02-18 | 1996-08-22 | Sel Alcatel Ag | Optisches Übertragungssystem für Kabelfernsehsignale und Video- und Telekommunikationssignale |
JP4093598B2 (ja) | 1995-03-14 | 2008-06-04 | 大関株式会社 | 新規なアルコールアセチルトランスフェラーゼ遺伝子 |
JP3171560B2 (ja) * | 1996-05-21 | 2001-05-28 | 仁 苅野 | ヘテロポリ酸触媒 |
DE19744212B4 (de) | 1997-09-30 | 2006-01-19 | Schering Ag | Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen |
US6303302B1 (en) | 1997-11-19 | 2001-10-16 | The Whitehead Institute For Biomedical Research | Regulation of fungal gene expression |
FR2774393B1 (fr) * | 1998-02-05 | 2000-03-24 | Hoechst Marion Roussel Inc | Souches de levure ayant le gene atf2 interrompu et leurs applications |
FR2799206A1 (fr) | 1999-10-05 | 2001-04-06 | Transgene Sa | Procede de production de steroides modifies par fermentation de levures |
FR2812884B1 (fr) | 2000-08-08 | 2002-11-08 | Aventis Pharma Sa | Levures modifiees et utilisations, notamment pour la production de derives steroidiens |
FR2820145B1 (fr) | 2001-01-31 | 2004-01-23 | Aventis Pharma Sa | Souche de levure produisant des steroides de facon autonome |
-
2001
- 2001-01-31 FR FR0101294A patent/FR2820145B1/fr not_active Expired - Lifetime
-
2002
- 2002-01-29 EP EP02708406A patent/EP1385980B1/fr not_active Expired - Lifetime
- 2002-01-29 IL IL15711802A patent/IL157118A0/xx unknown
- 2002-01-29 AT AT02708406T patent/ATE486134T1/de active
- 2002-01-29 KR KR1020037010173A patent/KR100882150B1/ko active IP Right Grant
- 2002-01-29 NZ NZ526381A patent/NZ526381A/en not_active IP Right Cessation
- 2002-01-29 DK DK02708406.0T patent/DK1385980T3/da active
- 2002-01-29 CA CA2435096A patent/CA2435096C/en not_active Expired - Lifetime
- 2002-01-29 WO PCT/FR2002/000348 patent/WO2002061109A2/fr active IP Right Grant
- 2002-01-29 BR BR0206809-5 patent/BRPI0206809B8/pt not_active IP Right Cessation
- 2002-01-29 MX MXPA03006031A patent/MXPA03006031A/es active IP Right Grant
- 2002-01-29 PT PT02708406T patent/PT1385980E/pt unknown
- 2002-01-29 DE DE60238114T patent/DE60238114D1/de not_active Expired - Lifetime
- 2002-01-29 ES ES02708406T patent/ES2354603T3/es not_active Expired - Lifetime
- 2002-01-29 US US10/470,673 patent/US7670829B2/en not_active Expired - Lifetime
- 2002-01-29 JP JP2002561663A patent/JP4620932B2/ja not_active Expired - Lifetime
- 2002-01-30 AR ARP020100328A patent/AR033857A1/es active IP Right Grant
-
2003
- 2003-07-17 NO NO20033242A patent/NO333769B1/no not_active IP Right Cessation
- 2003-07-25 ZA ZA200305772A patent/ZA200305772B/en unknown
- 2003-07-27 IL IL157118A patent/IL157118A/en unknown
-
2010
- 2010-01-07 US US12/683,642 patent/US7977065B2/en not_active Expired - Fee Related
-
2011
- 2011-01-12 CY CY20111100036T patent/CY1111099T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
FR2820145A1 (fr) | 2002-08-02 |
FR2820145B1 (fr) | 2004-01-23 |
KR20030082580A (ko) | 2003-10-22 |
ZA200305772B (en) | 2004-07-26 |
US7670829B2 (en) | 2010-03-02 |
IL157118A0 (en) | 2004-02-08 |
US20100112634A1 (en) | 2010-05-06 |
JP4620932B2 (ja) | 2011-01-26 |
CY1111099T1 (el) | 2015-06-11 |
CA2435096A1 (en) | 2002-08-08 |
AR033857A1 (es) | 2004-01-07 |
DK1385980T3 (da) | 2011-02-21 |
ATE486134T1 (de) | 2010-11-15 |
US7977065B2 (en) | 2011-07-12 |
US20040137556A1 (en) | 2004-07-15 |
BRPI0206809B1 (pt) | 2020-04-22 |
NO20033242D0 (no) | 2003-07-17 |
WO2002061109A2 (fr) | 2002-08-08 |
EP1385980B1 (fr) | 2010-10-27 |
NZ526381A (en) | 2005-07-29 |
JP2004528827A (ja) | 2004-09-24 |
CA2435096C (en) | 2012-11-13 |
BRPI0206809B8 (pt) | 2021-05-25 |
ES2354603T3 (es) | 2011-03-16 |
BR0206809A (pt) | 2004-02-03 |
WO2002061109A3 (fr) | 2003-11-27 |
NO20033242L (no) | 2003-09-04 |
PT1385980E (pt) | 2011-01-14 |
MXPA03006031A (es) | 2005-02-14 |
KR100882150B1 (ko) | 2009-02-06 |
EP1385980A2 (fr) | 2004-02-04 |
DE60238114D1 (de) | 2010-12-09 |
IL157118A (en) | 2010-11-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7977065B2 (en) | Yeast strains autonomously producing steroids | |
RU2242517C2 (ru) | Последовательность нуклеиновой кислоты, кодирующая белок, обладающий дельта-5,7 стерин, дельта-7 редуктазной активностью (варианты), белок из arabidopsis thaliana, обладающий указанной активностью (варианты), вектор экспрессии, способ клонирования нуклеиновой кислоты, диагностический зонд, способ получения белка, способы восстановления стерина (варианты), способ получения прегненолона (варианты), трансформированный дрожжевой штамм и способ выявления недостаточности дельта-5,7 стерин, дельта-7 редуктазы | |
DK175573B1 (da) | Anvendelse af en ekspressionskassette förende til biokemisk oxidation af steroider, sådanne ekspressionskassetter, | |
JP4443636B2 (ja) | 中断されたatf2遺伝子を有する酵母株及びそれらの利用 | |
IL154294A (en) | Modified yeast, their methods and uses, in particular for the production of steroid histories and non-cellular preparations | |
AU707957B2 (en) | DNA sequence coding for a protein of A. thaliana having a delta-5,7sterol,delta-7 reductase activity, delta7-red protein, production process, strains of transformed yeasts, uses |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK1K | Patent expired |