ATE486134T1 - Hefe stämme die autonom steroide produzieren - Google Patents

Hefe stämme die autonom steroide produzieren

Info

Publication number
ATE486134T1
ATE486134T1 AT02708406T AT02708406T ATE486134T1 AT E486134 T1 ATE486134 T1 AT E486134T1 AT 02708406 T AT02708406 T AT 02708406T AT 02708406 T AT02708406 T AT 02708406T AT E486134 T1 ATE486134 T1 AT E486134T1
Authority
AT
Austria
Prior art keywords
yeast strains
produce steroids
autonomously produce
autonomously
steroids
Prior art date
Application number
AT02708406T
Other languages
English (en)
Inventor
Roberto Spagnoli
Tilman Achstetter
Gilles Cauet
Eric Degryse
Bruno Dumas
Denis Pompon
Jacques Winter
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Application granted granted Critical
Publication of ATE486134T1 publication Critical patent/ATE486134T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15004—Steroid 11-beta-monooxygenase (1.14.15.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/005—Degradation of the lateral chains at position 17
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
    • C12P33/16—Acting at 17 position
    • C12P33/18—Hydroxylating at 17 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15006—Cholesterol monooxygenase (side-chain-cleaving) (1.14.15.6), i.e. cytochrome P450scc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/9901—Steroid 21-monooxygenase (1.14.99.10), i.e. steroid cytochrome P450 21-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
    • C12Y118/01002—Ferredoxin-NADP+ reductase (1.18.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Vascular Medicine (AREA)
  • Transplantation (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Psychology (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT02708406T 2001-01-31 2002-01-29 Hefe stämme die autonom steroide produzieren ATE486134T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0101294A FR2820145B1 (fr) 2001-01-31 2001-01-31 Souche de levure produisant des steroides de facon autonome
PCT/FR2002/000348 WO2002061109A2 (fr) 2001-01-31 2002-01-29 Souches de levures produisant des steroides de facon autonome

Publications (1)

Publication Number Publication Date
ATE486134T1 true ATE486134T1 (de) 2010-11-15

Family

ID=8859443

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02708406T ATE486134T1 (de) 2001-01-31 2002-01-29 Hefe stämme die autonom steroide produzieren

Country Status (20)

Country Link
US (2) US7670829B2 (de)
EP (1) EP1385980B1 (de)
JP (1) JP4620932B2 (de)
KR (1) KR100882150B1 (de)
AR (1) AR033857A1 (de)
AT (1) ATE486134T1 (de)
BR (1) BRPI0206809B8 (de)
CA (1) CA2435096C (de)
CY (1) CY1111099T1 (de)
DE (1) DE60238114D1 (de)
DK (1) DK1385980T3 (de)
ES (1) ES2354603T3 (de)
FR (1) FR2820145B1 (de)
IL (2) IL157118A0 (de)
MX (1) MXPA03006031A (de)
NO (1) NO333769B1 (de)
NZ (1) NZ526381A (de)
PT (1) PT1385980E (de)
WO (1) WO2002061109A2 (de)
ZA (1) ZA200305772B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2812884B1 (fr) 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
US20060177416A1 (en) 2003-10-14 2006-08-10 Medivas, Llc Polymer particle delivery compositions and methods of use
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
US20070160622A1 (en) * 2005-12-07 2007-07-12 Medivas, Llc Method for assembling a polymer-biologic delivery composition
FR2869914B1 (fr) * 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
EP1926780B1 (de) 2005-09-22 2013-08-14 Medivas, LLC Bis-( -amino)-diol-diester-haltige poly(esteramid) und poly(esterurethan)-zusammensetzungen und verwendungsverfahren
EP1933881B1 (de) * 2005-09-22 2019-03-13 Medivas, LLC Feste polymer-abgabezusammensetzungen und anwendungsverfahren dafür
JP5196498B2 (ja) * 2006-05-09 2013-05-15 メディバス エルエルシー 生分解性水溶性ポリマー
EP2178944A1 (de) * 2007-07-24 2010-04-28 Medivas, LLC Biologisch abbaubare polymere genübertragungszusammensetzungen und anwendungsverfahren dafür
CA2733686A1 (en) * 2008-08-13 2010-02-18 Medivas, Llc Aabb-poly(depsipeptide) biodegradable polymers and methods of use
JPWO2010079594A1 (ja) 2009-01-07 2012-06-21 三菱化学株式会社 ステロール側鎖切断酵素蛋白質およびその利用
FR2961218A1 (fr) 2010-06-10 2011-12-16 Sanofi Aventis Methode de detection de composes modulateurs du metabolisme du cholesterol
KR200458070Y1 (ko) * 2010-06-21 2012-01-19 주광진 양치 기능이 부가된 젖꼭지
WO2012095816A1 (en) 2011-01-14 2012-07-19 Sanofi Method for detecting the biological activity of alpha-synuclein
FR2976949B1 (fr) * 2011-06-21 2015-08-28 Sanofi Sa Procede de preparation de levures genetiquement transformees capables de produire une molecule d'interet a haut titre
US9873765B2 (en) 2011-06-23 2018-01-23 Dsm Ip Assets, B.V. Biodegradable polyesteramide copolymers for drug delivery
JP6045575B2 (ja) 2011-06-23 2016-12-14 ディーエスエム アイピー アセッツ ビー．ブイ． 薬物送達のための新規な生分解性ポリエステルアミドコポリマー
AU2014283301A1 (en) * 2013-06-17 2016-01-07 Sanofi Whole-cell system for cytochrome P450 monooxygenases biocatalysis
JP6720447B2 (ja) 2014-12-18 2020-07-08 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 酸感受性薬剤の送達のための薬剤送達系
EP3130680A1 (de) * 2015-08-11 2017-02-15 Universitat de Girona Verfahren zur detektion, nachverfolgung und/oder klassifikation von darmerkrankungen
US11693017B2 (en) 2015-09-18 2023-07-04 President And Fellows Of Harvard College Small molecule biosensors
CN108866142B (zh) * 2017-05-10 2022-08-16 中国科学院分子植物科学卓越创新中心 细胞色素p450、烟酰胺腺嘌呤二核苷酸-细胞色素p450还原酶及其应用
CN110903993A (zh) * 2019-12-20 2020-03-24 河北兰升生物科技有限公司 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用
CN114456964B (zh) * 2022-03-15 2023-02-03 陕西海斯夫生物工程有限公司 一种高产豆甾醇的重组解脂亚罗酵母、其构建方法、用于产豆甾醇的发酵培养基及应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6447380A (en) * 1987-08-19 1989-02-21 Agency Ind Science Techn Steroid-oxidizing yeast strain
IL90207A (en) * 1988-05-06 1994-07-31 Roussel Uclaf Biochemical oxidation of steroids and genetically engineered cells to be used therefor
EP0340878B1 (de) 1988-05-06 2001-05-16 Aventis Pharma S.A. Verfahren zur biochemischen Oxidation von Steroiden sowie dafür verwendbare, genetisch manipulierte Zellen
JPH0231680A (ja) 1988-07-22 1990-02-01 Agency Of Ind Science & Technol チトクロムＰ４５０ｃ↓２↓１産生酵母菌株
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
JPH02249488A (ja) 1989-03-22 1990-10-05 Sumitomo Chem Co Ltd チトクロムＰ４５０↓ｃ↓２↓１と酵母ＮＡＤＰＨ‐チトクロムＰ４５０還元酵素の融合酸化酵素、該酵素をコードする遺伝子および該酵素の製造方法
JP2645130B2 (ja) 1989-03-31 1997-08-25 日清製粉株式会社 ステロイド誘導体
FR2678611B1 (fr) 1991-07-04 1995-01-20 Roussel Uclaf Nouveaux esters pyrethrinouides de l'alcool 1,3,4,5,6,7-hexahydro 1,3-dioxo-2h-isoindol-2-yl-methylique, leur procede de preparation et leur application comme pesticides.
JP3363197B2 (ja) 1992-06-18 2003-01-08 麒麟麦酒株式会社 アルコールアセチルトランスフェラーゼ遺伝子およびその利用
US5547868A (en) 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes
IL116872A (en) 1995-02-15 2004-12-15 Aventis Pharma Sa Delta 5,7-Strol Reductase from Arbidopsis Thaliana
DE19505578A1 (de) 1995-02-18 1996-08-22 Sel Alcatel Ag Optisches Übertragungssystem für Kabelfernsehsignale und Video- und Telekommunikationssignale
JP4093598B2 (ja) 1995-03-14 2008-06-04 大関株式会社 新規なアルコールアセチルトランスフェラーゼ遺伝子
JP3171560B2 (ja) * 1996-05-21 2001-05-28 仁 苅野 ヘテロポリ酸触媒
DE19744212B4 (de) 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
US6303302B1 (en) 1997-11-19 2001-10-16 The Whitehead Institute For Biomedical Research Regulation of fungal gene expression
FR2774393B1 (fr) * 1998-02-05 2000-03-24 Hoechst Marion Roussel Inc Souches de levure ayant le gene atf2 interrompu et leurs applications
FR2799206A1 (fr) 1999-10-05 2001-04-06 Transgene Sa Procede de production de steroides modifies par fermentation de levures
FR2812884B1 (fr) 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome

Also Published As

Publication number Publication date
FR2820145A1 (fr) 2002-08-02
FR2820145B1 (fr) 2004-01-23
KR20030082580A (ko) 2003-10-22
ZA200305772B (en) 2004-07-26
US7670829B2 (en) 2010-03-02
IL157118A0 (en) 2004-02-08
US20100112634A1 (en) 2010-05-06
JP4620932B2 (ja) 2011-01-26
CY1111099T1 (el) 2015-06-11
CA2435096A1 (en) 2002-08-08
AR033857A1 (es) 2004-01-07
DK1385980T3 (da) 2011-02-21
US7977065B2 (en) 2011-07-12
US20040137556A1 (en) 2004-07-15
BRPI0206809B1 (pt) 2020-04-22
NO20033242D0 (no) 2003-07-17
WO2002061109A2 (fr) 2002-08-08
EP1385980B1 (de) 2010-10-27
NZ526381A (en) 2005-07-29
JP2004528827A (ja) 2004-09-24
CA2435096C (en) 2012-11-13
BRPI0206809B8 (pt) 2021-05-25
ES2354603T3 (es) 2011-03-16
BR0206809A (pt) 2004-02-03
WO2002061109A3 (fr) 2003-11-27
NO20033242L (no) 2003-09-04
PT1385980E (pt) 2011-01-14
MXPA03006031A (es) 2005-02-14
KR100882150B1 (ko) 2009-02-06
NO333769B1 (no) 2013-09-16
EP1385980A2 (de) 2004-02-04
DE60238114D1 (de) 2010-12-09
IL157118A (en) 2010-11-30

Similar Documents

Publication Publication Date Title
ATE486134T1 (de) Hefe stämme die autonom steroide produzieren
EP1627052A4 (de) Pyruvat produzierender hefestamm
DE502004001700D1 (de) Servolenkung
DE602004015206D1 (de) Elektrowerkzeug
DE60313103D1 (de) Kraftwerkzeug
EP1785235A4 (de) Schreitroboter
DE60301001D1 (de) Wässerige Siliciumdioxiddispersion
DE60302036D1 (de) Werkzeugvorrichtung
DE112004002444D2 (de) Servolenkung
ATA16952002A (de) Gebäude
DE60230225D1 (de) Elektrowerkzeuge die an feste oder abnehmbare Stromleitungen angeschlossen sind
DE50201303D1 (de) Werkzeug zum nietartigen verbinden
DE50311673D1 (de) Bohrhammer
DE60328579D1 (de) Hammermühle
GB2411162B (en) Vehicle lift
GB2399612B (en) Structural building assembly
FR2832480B1 (fr) Reducteur avec excentriques
HK1042927A1 (zh) 生產赤蘚糖醇的酵母菌株
AU2002242774B2 (en) Yeast strains autonomously producing steroids
GB0204357D0 (en) Mark-up language conversion
GB2399611B (en) Structural building assembly
DK200100324U3 (da) Dobbeltdækker bus
GB0303003D0 (en) Building structures
AU2003254420A1 (en) Building construction method
AU2003238095A1 (en) Panel used to produce formwork

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1385980

Country of ref document: EP