NO320428B1 - Rensing av en trippelheliksformasjon med et immobilisert oligonukleotid - Google Patents
Rensing av en trippelheliksformasjon med et immobilisert oligonukleotid Download PDFInfo
- Publication number
- NO320428B1 NO320428B1 NO19972710A NO972710A NO320428B1 NO 320428 B1 NO320428 B1 NO 320428B1 NO 19972710 A NO19972710 A NO 19972710A NO 972710 A NO972710 A NO 972710A NO 320428 B1 NO320428 B1 NO 320428B1
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- Prior art keywords
- dna
- sequence
- oligonucleotide
- plasmid
- seq
- Prior art date
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Classifications
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/902—Specified use of nanostructure
- Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
- Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9415162A FR2728264B1 (fr) | 1994-12-16 | 1994-12-16 | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
PCT/FR1995/001468 WO1996018744A2 (fr) | 1994-12-16 | 1995-11-08 | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
Publications (3)
Publication Number | Publication Date |
---|---|
NO972710L NO972710L (no) | 1997-06-12 |
NO972710D0 NO972710D0 (no) | 1997-06-12 |
NO320428B1 true NO320428B1 (no) | 2005-12-05 |
Family
ID=9469865
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO19972710A NO320428B1 (no) | 1994-12-16 | 1997-06-12 | Rensing av en trippelheliksformasjon med et immobilisert oligonukleotid |
Country Status (31)
Country | Link |
---|---|
US (2) | US6287762B1 (ko) |
EP (2) | EP0797682B1 (ko) |
JP (2) | JPH10510427A (ko) |
KR (1) | KR100431163B1 (ko) |
CN (1) | CN100591776C (ko) |
AT (1) | ATE233825T1 (ko) |
AU (1) | AU715630B2 (ko) |
BG (1) | BG64198B1 (ko) |
BR (1) | BR9510089A (ko) |
CA (1) | CA2208245C (ko) |
CY (1) | CY1110372T1 (ko) |
CZ (1) | CZ295474B6 (ko) |
DE (1) | DE69529842T2 (ko) |
DK (1) | DK0797682T3 (ko) |
ES (2) | ES2197928T3 (ko) |
FI (1) | FI120836B (ko) |
FR (1) | FR2728264B1 (ko) |
HU (1) | HU221062B1 (ko) |
IL (2) | IL171488A (ko) |
MX (1) | MX9703670A (ko) |
NO (1) | NO320428B1 (ko) |
NZ (1) | NZ297023A (ko) |
PL (1) | PL184430B1 (ko) |
PT (1) | PT797682E (ko) |
RO (1) | RO116970B1 (ko) |
RU (1) | RU2174125C2 (ko) |
SK (2) | SK288366B6 (ko) |
TW (1) | TW459043B (ko) |
UA (1) | UA72421C2 (ko) |
WO (1) | WO1996018744A2 (ko) |
ZA (1) | ZA9510756B (ko) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7038026B2 (en) | 2000-05-26 | 2006-05-02 | Centelion | Purification of a triple heli formation with an immobilized oligonucleotide |
WO1998037231A2 (de) * | 1997-02-22 | 1998-08-27 | Ruprecht-Karls-Universität Heidelberg | Markierung von nukleinsäuren mit speziellen probengemischen |
US20030105040A1 (en) * | 2001-11-13 | 2003-06-05 | Isis Pharmaceuticals Inc. | Antisense modulation of inhibitor-kappa B-R expression |
EP1151093A4 (en) * | 1999-02-04 | 2003-01-08 | Invitrogen Corp | ISOLATION OF NUCLEIC ACID MOLECULES |
US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
AU6345901A (en) | 2000-05-26 | 2001-12-11 | Aventis Pharma Sa | Purification of a triple heli formation with an immobilized oligonucleotide |
US20060008817A1 (en) | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
DE10104025B4 (de) * | 2001-01-31 | 2008-07-10 | Qiagen North American Holdings, Inc. | Verfahren zur Aufreinigung und anschließenden Amplifikation von Doppelstrang-DNA |
JP4444564B2 (ja) * | 2001-03-23 | 2010-03-31 | サントリオン | 三重螺旋相互作用によるターゲット二本鎖dna配列の精製及び検出方法 |
FR2822476B1 (fr) * | 2001-03-23 | 2004-04-02 | Aventis Pharma Sa | Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice |
KR100790764B1 (ko) | 2002-11-12 | 2008-01-03 | 인디애나 유니버시티 리서치 앤드 테크놀로지 코포레이션 | 조혈세포 집단에서 정지세포를 증대시키는 방법 |
AU2004272748C1 (en) * | 2003-09-17 | 2010-07-08 | Centelion | Method of preparation of pharmaceutically grade plasmid DNA |
DE602005018166D1 (de) | 2004-02-12 | 2010-01-21 | Population Genetics Technologi | Genetische analyse mittels sequenzspezifischem sortieren |
NZ549835A (en) * | 2004-04-19 | 2008-12-24 | Centelion | Method for purifying plasmid DNA |
EP1737945B1 (en) | 2004-04-19 | 2011-01-26 | Aventis Pharma S.A. | Method for purifying plasmid dna |
US8748168B2 (en) | 2004-08-16 | 2014-06-10 | Nature Technology Corp. | Strains of E. coli for plasmid DNA production |
US7407757B2 (en) * | 2005-02-10 | 2008-08-05 | Population Genetics Technologies | Genetic analysis by sequence-specific sorting |
CN105316314B (zh) * | 2014-07-29 | 2018-07-27 | 深圳新诺微环生物科技有限公司 | 一种高纯度的微环dna及其制备方法和应用 |
US10844425B2 (en) | 2017-03-24 | 2020-11-24 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying parainfluenza virus |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2540122B1 (fr) * | 1983-01-27 | 1985-11-29 | Centre Nat Rech Scient | Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application |
US5166315A (en) * | 1989-12-20 | 1992-11-24 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
US4766072A (en) * | 1985-07-17 | 1988-08-23 | Promega Corporation | Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence |
US5665541A (en) | 1986-10-28 | 1997-09-09 | The Johns Hopkins University | Formation of triple helix complexes for the detection of double stranded DNA sequences using oligomers which comprise an 8-modified purine base |
WO1989002436A1 (fr) | 1987-09-17 | 1989-03-23 | Appligene | Procede de fixation et de separation de molecules comportant au moins un fragment d'adn naturel ou synthetique |
US5352578A (en) | 1989-02-15 | 1994-10-04 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
AU5269590A (en) | 1989-03-10 | 1990-10-09 | Gene-Trak Systems | Immobilized oligonucleotide probes and uses therefor |
CA2015515C (en) * | 1990-01-03 | 1999-12-07 | Jean-Marie Saint-Remy | Pharmaceutical compositions containing antigen-antibody complexes and uses therefor |
AU9094991A (en) * | 1990-11-23 | 1992-06-25 | Gilead Sciences, Inc. | Triplex-forming oligomers containing modified bases |
EP0566670A4 (en) * | 1990-12-17 | 1993-12-08 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
WO1992013963A1 (en) | 1991-01-30 | 1992-08-20 | Hyman Edward D | Method for preparation of closed circular dna |
EP0533952A4 (en) | 1991-04-15 | 1993-07-07 | Sumitomo Electric Industries, Ltd. | Process for separating or extracting nucleic acid from specimen containing the same |
RU2186852C2 (ru) | 1991-12-24 | 2002-08-10 | Тепнел Медикал Лимитед | Способ выполнения манипуляции с последовательностью нуклеиновой кислоты, устройство для проведения манипуляций на последовательности нуклеиновой кислоты, проточный сосуд, твердый носитель для иммобилизации последовательности нуклеиновой кислоты, твердый носитель, способ получения носителя для иммобилизации последовательности нуклеиновой кислоты |
AU4544193A (en) | 1992-06-25 | 1994-01-24 | Microprobe Corporation | Solid supports for nucleic acid hybridization assays |
US5401632A (en) | 1992-07-16 | 1995-03-28 | Wisconsin Alumni Research Foundation | Triple helix purification and sequencing |
WO1994017086A1 (en) * | 1993-01-25 | 1994-08-04 | Apollon, Inc. | Gene regulation by targeting putative intramolecular triple helix |
-
1994
- 1994-12-16 FR FR9415162A patent/FR2728264B1/fr not_active Expired - Lifetime
-
1995
- 1995-08-11 UA UA97062835A patent/UA72421C2/uk unknown
- 1995-11-08 CZ CZ19971858A patent/CZ295474B6/cs not_active IP Right Cessation
- 1995-11-08 EP EP95940285A patent/EP0797682B1/fr not_active Expired - Lifetime
- 1995-11-08 CA CA2208245A patent/CA2208245C/fr not_active Expired - Lifetime
- 1995-11-08 DE DE69529842T patent/DE69529842T2/de not_active Expired - Lifetime
- 1995-11-08 EP EP02012081.2A patent/EP1281774B1/fr not_active Expired - Lifetime
- 1995-11-08 SK SK5104-2008A patent/SK288366B6/sk not_active IP Right Cessation
- 1995-11-08 US US08/860,038 patent/US6287762B1/en not_active Expired - Lifetime
- 1995-11-08 SK SK756-97A patent/SK286956B6/sk not_active IP Right Cessation
- 1995-11-08 DK DK95940285T patent/DK0797682T3/da active
- 1995-11-08 PT PT95940285T patent/PT797682E/pt unknown
- 1995-11-08 HU HU9701886A patent/HU221062B1/hu unknown
- 1995-11-08 NZ NZ297023A patent/NZ297023A/xx not_active IP Right Cessation
- 1995-11-08 KR KR1019970703997A patent/KR100431163B1/ko not_active IP Right Cessation
- 1995-11-08 CN CN95196817A patent/CN100591776C/zh not_active Expired - Lifetime
- 1995-11-08 JP JP8518319A patent/JPH10510427A/ja active Pending
- 1995-11-08 MX MX9703670A patent/MX9703670A/es unknown
- 1995-11-08 RU RU97111878/1497111878/14A patent/RU2174125C2/ru active
- 1995-11-08 AT AT95940285T patent/ATE233825T1/de active
- 1995-11-08 BR BR9510089A patent/BR9510089A/pt not_active Application Discontinuation
- 1995-11-08 RO RO97-01102A patent/RO116970B1/ro unknown
- 1995-11-08 ES ES95940285T patent/ES2197928T3/es not_active Expired - Lifetime
- 1995-11-08 PL PL95320697A patent/PL184430B1/pl unknown
- 1995-11-08 WO PCT/FR1995/001468 patent/WO1996018744A2/fr active Application Filing
- 1995-11-08 AU AU41789/96A patent/AU715630B2/en not_active Expired
- 1995-11-08 ES ES02012081.2T patent/ES2446983T3/es not_active Expired - Lifetime
- 1995-12-14 IL IL171488A patent/IL171488A/en not_active IP Right Cessation
- 1995-12-14 IL IL116398A patent/IL116398A/en not_active IP Right Cessation
- 1995-12-16 TW TW084113451A patent/TW459043B/zh not_active IP Right Cessation
- 1995-12-18 ZA ZA9510756A patent/ZA9510756B/xx unknown
-
1997
- 1997-06-12 NO NO19972710A patent/NO320428B1/no not_active IP Right Cessation
- 1997-06-13 FI FI972526A patent/FI120836B/fi not_active IP Right Cessation
- 1997-06-16 BG BG101620A patent/BG64198B1/bg unknown
-
2000
- 2000-05-26 US US09/580,923 patent/US6319672B1/en not_active Expired - Lifetime
-
2008
- 2008-03-07 CY CY20081100271T patent/CY1110372T1/el unknown
- 2008-10-16 JP JP2008267538A patent/JP2009045070A/ja active Pending
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