NO310884B1 - Par av oligonukleotidprimere, oligonukleotidprobe, anvendelse av dem for påvisning, samt analysesett som omfatter par avoligonukleotidprimere og valgfritt minst ±n oligonukleotidprobe - Google Patents
Par av oligonukleotidprimere, oligonukleotidprobe, anvendelse av dem for påvisning, samt analysesett som omfatter par avoligonukleotidprimere og valgfritt minst ±n oligonukleotidprobe Download PDFInfo
- Publication number
- NO310884B1 NO310884B1 NO19923201A NO923201A NO310884B1 NO 310884 B1 NO310884 B1 NO 310884B1 NO 19923201 A NO19923201 A NO 19923201A NO 923201 A NO923201 A NO 923201A NO 310884 B1 NO310884 B1 NO 310884B1
- Authority
- NO
- Norway
- Prior art keywords
- seq
- sequence
- probes
- nucleic acid
- probe
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title claims description 41
- 108020005187 Oligonucleotide Probes Proteins 0.000 title claims description 20
- 239000002751 oligonucleotide probe Substances 0.000 title claims description 20
- 239000003155 DNA primer Substances 0.000 title claims description 8
- 238000003149 assay kit Methods 0.000 title description 10
- 239000000523 sample Substances 0.000 claims abstract description 221
- 241000894007 species Species 0.000 claims abstract description 104
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 68
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 55
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 55
- 108020004465 16S ribosomal RNA Proteins 0.000 claims abstract description 32
- 241000186359 Mycobacterium Species 0.000 claims abstract description 18
- 102000042567 non-coding RNA Human genes 0.000 claims abstract description 13
- 239000013615 primer Substances 0.000 claims description 107
- 238000009396 hybridization Methods 0.000 claims description 66
- 230000003321 amplification Effects 0.000 claims description 65
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 65
- 238000003752 polymerase chain reaction Methods 0.000 claims description 49
- 108091034117 Oligonucleotide Proteins 0.000 claims description 27
- 230000000295 complement effect Effects 0.000 claims description 24
- 239000013641 positive control Substances 0.000 claims description 23
- 238000004458 analytical method Methods 0.000 claims description 18
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 13
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 5
- 238000011144 upstream manufacturing Methods 0.000 claims description 4
- 108020004414 DNA Proteins 0.000 description 67
- 238000000034 method Methods 0.000 description 49
- 239000000047 product Substances 0.000 description 39
- 238000006243 chemical reaction Methods 0.000 description 36
- 238000003556 assay Methods 0.000 description 32
- 239000012528 membrane Substances 0.000 description 25
- 239000000243 solution Substances 0.000 description 22
- 239000000758 substrate Substances 0.000 description 20
- 239000000872 buffer Substances 0.000 description 19
- 239000003153 chemical reaction reagent Substances 0.000 description 17
- 108090000623 proteins and genes Proteins 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 239000013612 plasmid Substances 0.000 description 14
- 239000002987 primer (paints) Substances 0.000 description 14
- 230000002441 reversible effect Effects 0.000 description 14
- 102100031780 Endonuclease Human genes 0.000 description 13
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- 239000002585 base Substances 0.000 description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
- 239000004677 Nylon Substances 0.000 description 10
- 238000012408 PCR amplification Methods 0.000 description 10
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 10
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 10
- 238000004925 denaturation Methods 0.000 description 10
- 230000036425 denaturation Effects 0.000 description 10
- 229920001778 nylon Polymers 0.000 description 10
- 238000003786 synthesis reaction Methods 0.000 description 10
- 238000010839 reverse transcription Methods 0.000 description 9
- 201000008827 tuberculosis Diseases 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 241000187494 Mycobacterium xenopi Species 0.000 description 8
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000005406 washing Methods 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 238000006116 polymerization reaction Methods 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- -1 polymerase Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000001226 triphosphate Substances 0.000 description 6
- 235000011178 triphosphate Nutrition 0.000 description 6
- 230000006820 DNA synthesis Effects 0.000 description 5
- 108060004795 Methyltransferase Proteins 0.000 description 5
- 108010006785 Taq Polymerase Proteins 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 241000187489 Mycobacterium simiae Species 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 238000007834 ligase chain reaction Methods 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- 229940035893 uracil Drugs 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000186367 Mycobacterium avium Species 0.000 description 3
- 241000187484 Mycobacterium gordonae Species 0.000 description 3
- 241000186364 Mycobacterium intracellulare Species 0.000 description 3
- 241000186363 Mycobacterium kansasii Species 0.000 description 3
- 241000186362 Mycobacterium leprae Species 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 108010057517 Strep-avidin conjugated horseradish peroxidase Proteins 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 3
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 3
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 3
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 2
- QYYMDNHUJFIDDQ-UHFFFAOYSA-N 5-chloro-2-methyl-1,2-thiazol-3-one;2-methyl-1,2-thiazol-3-one Chemical compound CN1SC=CC1=O.CN1SC(Cl)=CC1=O QYYMDNHUJFIDDQ-UHFFFAOYSA-N 0.000 description 2
- 101710122462 65 kDa protein Proteins 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- 241000713838 Avian myeloblastosis virus Species 0.000 description 2
- 101100148606 Caenorhabditis elegans pst-1 gene Proteins 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000186366 Mycobacterium bovis Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 108010085671 Thermus thermophilus DNA polymerase Proteins 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 108010030694 avidin-horseradish peroxidase complex Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 239000005549 deoxyribonucleoside Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 206010013023 diphtheria Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000000155 isotopic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 2
- VADKRMSMGWJZCF-UHFFFAOYSA-N 2-bromophenol Chemical compound OC1=CC=CC=C1Br VADKRMSMGWJZCF-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000002430 Multiple chemical sensitivity Diseases 0.000 description 1
- 241000187472 Mycobacterium chitae Species 0.000 description 1
- 241000187495 Mycobacterium terrae Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 102000001218 Rec A Recombinases Human genes 0.000 description 1
- 108010055016 Rec A Recombinases Proteins 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108010020713 Tth polymerase Proteins 0.000 description 1
- SWPYNTWPIAZGLT-UHFFFAOYSA-N [amino(ethoxy)phosphanyl]oxyethane Chemical compound CCOP(N)OCC SWPYNTWPIAZGLT-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 230000007515 enzymatic degradation Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 229940094991 herring sperm dna Drugs 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011850 initial investigation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- PRZSXZWFJHEZBJ-UHFFFAOYSA-N thymol blue Chemical compound C1=C(O)C(C(C)C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C(=CC(O)=C(C(C)C)C=2)C)=C1C PRZSXZWFJHEZBJ-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- NLIVDORGVGAOOJ-MAHBNPEESA-M xylene cyanol Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(\C=1C(=CC(OS([O-])=O)=CC=1)OS([O-])=O)=C\1C=C(C)\C(=[NH+]/CC)\C=C/1 NLIVDORGVGAOOJ-MAHBNPEESA-M 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US74670491A | 1991-08-15 | 1991-08-15 | |
US07/915,922 US5422242A (en) | 1991-08-15 | 1992-07-17 | Mycobacterium primers and probes |
Publications (3)
Publication Number | Publication Date |
---|---|
NO923201D0 NO923201D0 (no) | 1992-08-14 |
NO923201L NO923201L (no) | 1993-02-16 |
NO310884B1 true NO310884B1 (no) | 2001-09-10 |
Family
ID=27114638
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO19923201A NO310884B1 (no) | 1991-08-15 | 1992-08-14 | Par av oligonukleotidprimere, oligonukleotidprobe, anvendelse av dem for påvisning, samt analysesett som omfatter par avoligonukleotidprimere og valgfritt minst ±n oligonukleotidprobe |
Country Status (15)
Country | Link |
---|---|
EP (1) | EP0528306B1 (de) |
JP (1) | JP2675723B2 (de) |
CN (1) | CN1076397C (de) |
AT (1) | ATE186749T1 (de) |
AU (1) | AU659657B2 (de) |
CA (1) | CA2075847C (de) |
DE (1) | DE69230305T2 (de) |
DK (1) | DK0528306T3 (de) |
ES (1) | ES2140400T3 (de) |
FI (1) | FI106213B (de) |
GR (1) | GR3032597T3 (de) |
IL (1) | IL102765A (de) |
NO (1) | NO310884B1 (de) |
NZ (1) | NZ243921A (de) |
PT (1) | PT528306E (de) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0586112A3 (en) * | 1992-08-14 | 1994-09-14 | Pharma Gen S A | Control of pcr mediated detection of micro-organisms |
CA2121659C (en) * | 1993-05-11 | 2000-11-28 | Michael C. Little | Sample processing method for mycobacteria |
SG63589A1 (en) * | 1993-05-14 | 1999-03-30 | Johnson & Johnson Clin Diag | Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures |
US6136529A (en) * | 1993-09-03 | 2000-10-24 | Gen-Probe Incorporated | Nucleic acid probes to Mycobacterium avium complex |
JPH10500567A (ja) * | 1994-05-13 | 1998-01-20 | アボツト・ラボラトリーズ | マイコバクテリアの検出用材料及び検出方法 |
US5712095A (en) * | 1994-06-16 | 1998-01-27 | Becton Dickinson And Company | Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors |
FR2721617B1 (fr) * | 1994-06-24 | 1996-09-06 | Pasteur Institut | Fragments d'acides nucléiques, dérivés du génome de Mycobacterium xenopi et leurs applications. |
US5656427A (en) * | 1994-08-29 | 1997-08-12 | Gen-Probe Incorporated | Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid |
CA2163393C (en) * | 1994-11-30 | 2003-04-22 | Colleen Marie Nycz | Amplification and detection of mycobacteria nucleic acids |
US5925518A (en) * | 1995-05-19 | 1999-07-20 | Akzo Nobel N.V. | Nucleic acid primers for amplification of a mycobacteria RNA template |
US5795722A (en) * | 1997-03-18 | 1998-08-18 | Visible Genetics Inc. | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
DE19616750A1 (de) * | 1996-04-26 | 1997-11-06 | Newlab Diagnostic Systems Gmbh | Verfahren zum Nachweis von Mikroorganismen in Gemischen |
US5846718A (en) * | 1996-05-31 | 1998-12-08 | The Johns Hopkins University | Identification of pyrazinamide-resistant mycobacteria and methods for treating mycobacterial infections |
ES2129365B1 (es) * | 1997-07-18 | 2000-04-01 | Pharmagen S A | Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. |
US5985569A (en) * | 1997-09-26 | 1999-11-16 | Becton, Dickinson And Company | Primers for amplification of a genus specific sequence of the mycobacterium 16S rRNA gene |
PL202844B1 (pl) * | 1998-04-07 | 2009-07-31 | Corixa Corp | Immunogeniczny polipeptyd, polinukleotyd, polipeptyd, kompozycja farmaceutyczna, wektor ekspresji, kompozycja szczepionki, polipeptyd do stosowania w leczeniu lub zapobieganiu infekcjom Mycobacterium tuberculosis, polinukleotyd do stosowania w leczeniu lub zapobieganiu infekcjom Mycobacterium tuberculosis i sposób wytwarzania polipeptydu |
JP2003516765A (ja) | 1999-12-15 | 2003-05-20 | ジェン−プローブ・インコーポレーテッド | 鳥型結核菌(Mycobacteriumavium)複合体種の検出のための方法および組成物 |
US6664081B2 (en) | 1999-12-17 | 2003-12-16 | Gen-Probe Incorporated | Nucleic acid amplification and detection of mycobacterium species |
EP1242633B1 (de) | 1999-12-17 | 2011-01-12 | Gen-Probe Incorporated | Nukleinsäureamplifizierung und detektion von mykobacterium-spezies |
KR100433260B1 (ko) * | 2000-09-15 | 2004-05-24 | 주식회사 에스제이하이테크 | 멀티플렉스 pcr 방법 및 이를 이용한 마이코박테리아동정용 키트 및 올리고 뉴클레오티드 |
US7101663B2 (en) * | 2001-03-02 | 2006-09-05 | University of Pittsburgh—of the Commonwealth System of Higher Education | PCR method |
DE10215238C1 (de) * | 2002-04-06 | 2003-08-14 | Cytonet Gmbh & Co Kg | Nachweis von Mykobakterien in klinischem Material |
JP4304976B2 (ja) | 2002-12-19 | 2009-07-29 | 東ソー株式会社 | リボゾームrnaを標的とした抗酸菌の検出法 |
JP4769041B2 (ja) * | 2004-07-28 | 2011-09-07 | 株式会社ビー・エム・エル | 抗酸菌属細菌同定キット |
CN1311085C (zh) * | 2004-10-19 | 2007-04-18 | 中国人民解放军第三○九医院 | 分枝杆菌分子菌种鉴定试剂盒的制备及其应用 |
JP2011062088A (ja) * | 2009-09-15 | 2011-03-31 | Ihi Corp | レジオネラ菌検出方法 |
WO2011052586A1 (ja) * | 2009-10-29 | 2011-05-05 | 日本碍子株式会社 | 標的核酸の検出方法 |
CN102618625B (zh) * | 2011-01-27 | 2013-09-11 | 博奥生物有限公司 | 一种检测结核分枝杆菌耐药性的方法和试剂盒 |
JP6299660B2 (ja) * | 2014-05-12 | 2018-03-28 | 三菱ケミカル株式会社 | 菌叢解析方法と菌叢解析用デバイス |
CN110095598A (zh) * | 2019-04-08 | 2019-08-06 | 北京大学 | 一种基于磁性微球快速检测化学物质内分泌干扰活性的试剂盒及方法 |
WO2021124960A1 (ja) | 2019-12-18 | 2021-06-24 | 富士フイルム和光純薬株式会社 | マイコバクテリウム・ツベルクローシス、マイコバクテリウム・アビウム及びマイコバクテリウム・イントラセルラーを検出するためのプライマーセット及びこれを用いた方法、並びにそのための試薬キット |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE81145B1 (en) * | 1989-04-20 | 2000-05-03 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
CA2016553A1 (en) * | 1989-05-16 | 1990-11-16 | Dyann F. Wirth | Dna hybridization probes for identification of mycobacteria |
JPH03164199A (ja) * | 1989-08-09 | 1991-07-16 | Shima Kenkyusho:Kk | 結核菌の迅速同定方法及び同定用試薬キット |
-
1992
- 1992-08-08 ES ES92113540T patent/ES2140400T3/es not_active Expired - Lifetime
- 1992-08-08 EP EP92113540A patent/EP0528306B1/de not_active Expired - Lifetime
- 1992-08-08 AT AT92113540T patent/ATE186749T1/de active
- 1992-08-08 PT PT92113540T patent/PT528306E/pt unknown
- 1992-08-08 DK DK92113540T patent/DK0528306T3/da active
- 1992-08-08 DE DE69230305T patent/DE69230305T2/de not_active Expired - Lifetime
- 1992-08-10 IL IL102765A patent/IL102765A/xx not_active IP Right Cessation
- 1992-08-11 AU AU20961/92A patent/AU659657B2/en not_active Expired
- 1992-08-12 NZ NZ243921A patent/NZ243921A/en unknown
- 1992-08-12 CA CA002075847A patent/CA2075847C/en not_active Expired - Lifetime
- 1992-08-14 JP JP4258819A patent/JP2675723B2/ja not_active Expired - Lifetime
- 1992-08-14 NO NO19923201A patent/NO310884B1/no not_active IP Right Cessation
- 1992-08-14 FI FI923660A patent/FI106213B/fi not_active IP Right Cessation
- 1992-08-14 CN CN92110638A patent/CN1076397C/zh not_active Expired - Lifetime
-
2000
- 2000-02-09 GR GR20000400295T patent/GR3032597T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
ES2140400T3 (es) | 2000-03-01 |
EP0528306A3 (en) | 1993-09-15 |
GR3032597T3 (en) | 2000-05-31 |
JP2675723B2 (ja) | 1997-11-12 |
DK0528306T3 (da) | 2000-04-25 |
CA2075847A1 (en) | 1993-02-16 |
CA2075847C (en) | 2002-04-23 |
NO923201L (no) | 1993-02-16 |
AU2096192A (en) | 1993-04-08 |
CN1071955A (zh) | 1993-05-12 |
IL102765A (en) | 1997-03-18 |
DE69230305T2 (de) | 2000-07-20 |
PT528306E (pt) | 2000-05-31 |
IL102765A0 (en) | 1993-01-31 |
JPH06261757A (ja) | 1994-09-20 |
AU659657B2 (en) | 1995-05-25 |
DE69230305D1 (de) | 1999-12-23 |
FI106213B (fi) | 2000-12-15 |
EP0528306B1 (de) | 1999-11-17 |
NZ243921A (en) | 1994-01-26 |
CN1076397C (zh) | 2001-12-19 |
FI923660A (fi) | 1993-02-16 |
NO923201D0 (no) | 1992-08-14 |
ATE186749T1 (de) | 1999-12-15 |
FI923660A0 (fi) | 1992-08-14 |
EP0528306A2 (de) | 1993-02-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
NO310884B1 (no) | Par av oligonukleotidprimere, oligonukleotidprobe, anvendelse av dem for påvisning, samt analysesett som omfatter par avoligonukleotidprimere og valgfritt minst ±n oligonukleotidprobe | |
US5422242A (en) | Mycobacterium primers and probes | |
JP2709256B2 (ja) | マイコバクテリアプローブ | |
US5593836A (en) | Primers and probes for detecting Pneumocystis carinii | |
CA2126250C (en) | Method, reagents and kits for the detection of neisseria gonorrhoeae | |
EP0716714A1 (de) | Oligonukleotide und verfahren zum nachweis von chlamydian trachomatis | |
JPH10500023A (ja) | 結核菌検出用材料及び検出方法 | |
EP0687737B1 (de) | Nachweis von Treponema Pallidum und Haemophilus Ducreyi | |
EP0592894B1 (de) | Von der SOD Familie abgeleitete Oligonukleotide | |
US5453355A (en) | Oligonucleotides and methods for the detection of Neisseria gonorrhoeae | |
WO2011091330A1 (en) | Probes for detecting the presence of trichomonas vaginalis in a sample | |
US7879581B2 (en) | Nucleic acid amplification and detection of mycobacterium species | |
KR100388548B1 (ko) | 알이피 13 이 12 반복서열의 피시알 증폭을 이용한결핵균의 검출방법 | |
WO1997008340A1 (en) | Amplification and detection of mycobacterium avium complex species | |
EP0479117A1 (de) | Verfahren und Reagentien für die Identifikation von Bakterien | |
CA2389523C (en) | Nucleic acid amplification and detection of mycobacterium xenopi 16s ribosomal rna (rrna) or dna encoding 16s rrna in a biological sample | |
WO1991014002A2 (en) | Method for diagnosis of lyme disease | |
AU2007266857B2 (en) | Mycoplasma genitalium detection assay based on the MG219 gene |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK1K | Patent expired |