NO20053385L - IL-21-produksjon i prokaryote verter. - Google Patents

IL-21-produksjon i prokaryote verter.

Info

Publication number
NO20053385L
NO20053385L NO20053385A NO20053385A NO20053385L NO 20053385 L NO20053385 L NO 20053385L NO 20053385 A NO20053385 A NO 20053385A NO 20053385 A NO20053385 A NO 20053385A NO 20053385 L NO20053385 L NO 20053385L
Authority
NO
Norway
Prior art keywords
coli
production
expression
prokaryotic hosts
vectors
Prior art date
Application number
NO20053385A
Other languages
English (en)
Other versions
NO334561B1 (no
Inventor
Susan D Holderman
Chung Chan
Bruce L Zamost
Douglas C Covert
Hong Y Liu
Karen S De Jongh
Jeffrey D Meyer
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of NO20053385L publication Critical patent/NO20053385L/no
Publication of NO334561B1 publication Critical patent/NO334561B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Ekspresjonsvektorene og fremgangsmåtene som benytter et E.coli-uttrykkingssystem for storskalaproduksjon av IL-21 er beskrevet. Vektorene benytter den IL-21-kodende sekvensen med spesifikke endringer i nukleotider for å optimalisere kodons og mRNA-sekundærstruktur for translatering i E.coli. Ved å benytte ekspresjonsvektorene ble IL-21-genet produsert i E.coli til et nivå på større enn I g/I i tilsatt ladningsfermentering. Også inkludert er OmpTmanglende E.coli-stamme fransformert med en IL-21 -ekspresj onsvektor.
NO20053385A 2002-12-13 2005-07-12 Ekspresjonsvektor for fremstilling av IL-21-protein, prokaryot vertcelle for IL-21-produksjon og fremgangsmåte for fremstilling av IL-21-proteiner. NO334561B1 (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43344802P 2002-12-13 2002-12-13
US43345202P 2002-12-13 2002-12-13
PCT/US2003/039764 WO2004055168A2 (en) 2002-12-13 2003-12-12 Il-21 production in prokaryotic hosts

Publications (2)

Publication Number Publication Date
NO20053385L true NO20053385L (no) 2005-07-12
NO334561B1 NO334561B1 (no) 2014-04-07

Family

ID=32600150

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20053385A NO334561B1 (no) 2002-12-13 2005-07-12 Ekspresjonsvektor for fremstilling av IL-21-protein, prokaryot vertcelle for IL-21-produksjon og fremgangsmåte for fremstilling av IL-21-proteiner.

Country Status (17)

Country Link
US (4) US7250274B2 (no)
EP (1) EP1573006B1 (no)
JP (1) JP4664684B2 (no)
KR (1) KR101098897B1 (no)
AT (1) ATE448298T1 (no)
AU (1) AU2003302250B2 (no)
BR (1) BR0317243A (no)
CA (1) CA2507817C (no)
DE (1) DE60330044D1 (no)
DK (1) DK1573006T3 (no)
ES (1) ES2334127T3 (no)
IL (2) IL168697A (no)
MX (1) MXPA05006117A (no)
NO (1) NO334561B1 (no)
PL (1) PL209009B1 (no)
RU (1) RU2354703C2 (no)
WO (1) WO2004055168A2 (no)

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US6307024B1 (en) 1999-03-09 2001-10-23 Zymogenetics, Inc. Cytokine zalpha11 Ligand
US20050124044A1 (en) * 2003-06-19 2005-06-09 Cunningham Mark R. Interleukin-21 analogs
EP2263684A1 (en) 2003-10-10 2010-12-22 Novo Nordisk A/S IL-21 derivatives
US20060228331A1 (en) 2003-10-10 2006-10-12 Novo Nordisk A/S IL-21 Derivatives and variants
EP2633866A3 (en) * 2003-10-17 2013-12-18 Novo Nordisk A/S Combination therapy
JP4982373B2 (ja) * 2004-05-20 2012-07-25 ザイモジェネティクス， インコーポレイテッド Ｉｌ−２１およびモノクローナル抗体治療を用いる癌を処置する方法
US8034326B2 (en) * 2005-04-18 2011-10-11 Novo Nordisk A/S IL-21 variants
ATE515566T1 (de) * 2005-05-26 2011-07-15 Cytos Biotechnology Ag Skalierbares fermentationsverfahren
WO2006131515A2 (en) * 2005-06-06 2006-12-14 Novo Nordisk A/S Stabilised il-21 compositions
US7858747B2 (en) 2005-08-30 2010-12-28 Novo Nordisk A/S Expression of proteins in E.coli
ES2360728T3 (es) 2005-09-22 2011-06-08 Inserm (Institut National De La Santé Et De La Recherche Medicale) Métodos de utilización de il-21.
DK1931704T3 (da) * 2005-10-04 2011-03-14 Zymogenetics L L C Frembringelse og rensning af IL-29
US20070122413A1 (en) * 2005-11-28 2007-05-31 Sivakumar Pallavur V Il-21 antagonists
JP5401097B2 (ja) 2005-12-23 2014-01-29 ノボ・ノルデイスク・エー／エス 分取逆相クロマトグラフィー（ｒｐｃ）を使用するタンパク質精製
SG174013A1 (en) * 2006-07-27 2011-09-29 Wyeth Corp High-cell density fed-batch fermentation process for producing recombinant protein
CA2666426A1 (en) 2006-10-26 2008-05-02 Novo Nordisk A/S Il-21 variants
WO2008074863A1 (en) 2006-12-21 2008-06-26 Novo Nordisk A/S Interleukin-21 variants with altered binding to the il-21 receptor
EP2118121A1 (en) * 2007-03-01 2009-11-18 Novo Nordisk A/S Expression of proteins in e. coli
JP5358840B2 (ja) * 2007-11-24 2013-12-04 独立行政法人産業技術総合研究所 Ｇｆｐ（緑色蛍光蛋白質）の機能賦活・回復方法
RU2504552C2 (ru) 2007-12-07 2014-01-20 Займодженетикс, Инк. Моноклональные антитела против il-21 человека
US9927440B2 (en) * 2009-11-25 2018-03-27 Duke University Protein engineering
WO2011149917A1 (en) * 2010-05-25 2011-12-01 Adlyfe, Inc. STABILIZED AMYLOID- β OLIGOMERS AND USES THEREOF
WO2013169693A1 (en) 2012-05-09 2013-11-14 Bristol-Myers Squibb Company Methods of treating cancer using an il-21 polypeptide and an anti-pd-1 antibody
JP6086378B2 (ja) * 2012-11-30 2017-03-01 国立大学法人お茶の水女子大学 組み換えヒト膵臓リパーゼの調製方法
WO2015178466A1 (ja) * 2014-05-21 2015-11-26 味の素株式会社 フィブロイン様タンパク質の製造法
RU2620073C2 (ru) * 2015-06-02 2017-05-22 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России Оптимизированный ген, кодирующий рекомбинантный белок - аналог интерферона альфа-17 человека
RU2614264C2 (ru) * 2015-06-19 2017-03-24 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России Оптимизированный ген, кодирующий рекомбинантный белок - аналог интерферона бета человека
RU2614124C9 (ru) * 2015-10-01 2020-04-22 Федеральное государственное бюджетное учреждение "Государственный научный центр "Институт иммунологии" Федерального медико-биологического агентства России (ФГБУ "ГНЦ Институт иммунологии" ФМБА России) Оптимизированный ген, кодирующий рекомбинантный белок ипфiii
JP2017110001A (ja) * 2015-12-16 2017-06-22 株式会社 資生堂 タブレット型凍結乾燥化粧料
JP6404405B1 (ja) * 2017-06-14 2018-10-10 株式会社 資生堂 タブレット型凍結乾燥化粧料
US20210145705A1 (en) * 2017-06-19 2021-05-20 Shiseido Company, Ltd. Tablet-type freeze-dried cosmetic
KR20220080178A (ko) * 2019-10-14 2022-06-14 인바이오스 엔.브이. 숙주 세포에서 바이오산물의 생산
AU2021286574A1 (en) * 2020-06-09 2023-01-19 Icosavax, Inc. Method of making virus-like particle

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100408229B1 (ko) * 1996-10-04 2003-12-01 암겐 인코포레이티드 Ｍｐｌ 리간드를 함유하는 제약학적 조성물
US6057128A (en) 1998-03-17 2000-05-02 Genetics Institute, Inc. MU-1, member of the cytokine receptor family
CN1927883B (zh) * 1999-03-09 2011-09-21 津莫吉尼蒂克斯公司 新的细胞因子zalpha11配体
US6307024B1 (en) 1999-03-09 2001-10-23 Zymogenetics, Inc. Cytokine zalpha11 Ligand
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
CA2481304A1 (en) 2002-03-27 2003-10-09 Patrick Hwu Method for treating cancer in humans
AU2003230834A1 (en) 2002-04-09 2003-10-27 Beth Israel Deaconess Medical Center, Inc. Antagonists of il-21 and modulation of il-21-mediated t cell responses
BR0315134A (pt) 2002-10-11 2005-08-16 Novo Nordisk As Usos de il-21 e de um polipeptìdeo de il-21
CN1513993A (zh) 2002-12-31 2004-07-21 北京博泰迪生物工程科技开发有限公司 中国人基因组cDNA文库白细胞介素21的编码基因序列及其蛋白质的氨基酸序列
US20050124044A1 (en) 2003-06-19 2005-06-09 Cunningham Mark R. Interleukin-21 analogs
JP2007512008A (ja) 2003-11-24 2007-05-17 ノボ　ノルディスク　アクティーゼルスカブ 融合タンパク質及び該タンパク質の切断方法

Also Published As

Publication number Publication date
US7745176B2 (en) 2010-06-29
CA2507817A1 (en) 2004-07-01
DK1573006T3 (da) 2010-03-22
PL377291A1 (pl) 2006-01-23
KR101098897B1 (ko) 2011-12-26
AU2003302250B2 (en) 2009-08-27
WO2004055168A3 (en) 2005-06-02
ATE448298T1 (de) 2009-11-15
US20070048798A1 (en) 2007-03-01
MXPA05006117A (es) 2005-09-30
AU2003302250A1 (en) 2004-07-09
JP2006509525A (ja) 2006-03-23
US20070048842A1 (en) 2007-03-01
EP1573006B1 (en) 2009-11-11
US7622561B2 (en) 2009-11-24
BR0317243A (pt) 2005-11-01
WO2004055168A2 (en) 2004-07-01
US7767199B2 (en) 2010-08-03
ES2334127T3 (es) 2010-03-05
EP1573006A4 (en) 2006-06-14
US7250274B2 (en) 2007-07-31
EP1573006A2 (en) 2005-09-14
NO334561B1 (no) 2014-04-07
RU2354703C2 (ru) 2009-05-10
PL209009B1 (pl) 2011-07-29
CA2507817C (en) 2014-04-22
DE60330044D1 (de) 2009-12-24
IL168697A (en) 2011-10-31
US20070048263A1 (en) 2007-03-01
US20060134754A1 (en) 2006-06-22
KR20050090393A (ko) 2005-09-13
IL198074A0 (en) 2011-08-01
RU2005117338A (ru) 2006-03-20
JP4664684B2 (ja) 2011-04-06

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