NO20006675D0 - Fremgangsmåte for fremstilling av höyt varierte bibliotek - Google Patents

Fremgangsmåte for fremstilling av höyt varierte bibliotek

Info

Publication number
NO20006675D0
NO20006675D0 NO20006675A NO20006675A NO20006675D0 NO 20006675 D0 NO20006675 D0 NO 20006675D0 NO 20006675 A NO20006675 A NO 20006675A NO 20006675 A NO20006675 A NO 20006675A NO 20006675 D0 NO20006675 D0 NO 20006675D0
Authority
NO
Norway
Prior art keywords
nucleic acid
library
fragments
templates
strand
Prior art date
Application number
NO20006675A
Other languages
English (en)
Norwegian (no)
Other versions
NO20006675L (no
Inventor
Richard Wagner
Martin C Wright
Brent Kreider
Original Assignee
Phylos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phylos Inc filed Critical Phylos Inc
Publication of NO20006675L publication Critical patent/NO20006675L/no
Publication of NO20006675D0 publication Critical patent/NO20006675D0/no

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
NO20006675A 1998-06-29 2000-12-28 Fremgangsmåte for fremstilling av höyt varierte bibliotek NO20006675D0 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9097098P 1998-06-29 1998-06-29
PCT/US1999/014776 WO2000000632A1 (en) 1998-06-29 1999-06-29 Methods for generating highly diverse libraries

Publications (2)

Publication Number Publication Date
NO20006675L NO20006675L (no) 2000-12-28
NO20006675D0 true NO20006675D0 (no) 2000-12-28

Family

ID=22225171

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20006675A NO20006675D0 (no) 1998-06-29 2000-12-28 Fremgangsmåte for fremstilling av höyt varierte bibliotek

Country Status (13)

Country Link
EP (1) EP1092039B1 (enExample)
JP (1) JP4700805B2 (enExample)
KR (1) KR20010071613A (enExample)
CN (1) CN1308683A (enExample)
AT (1) ATE547532T1 (enExample)
AU (1) AU761570B2 (enExample)
CA (1) CA2331926C (enExample)
ES (1) ES2381824T3 (enExample)
IL (1) IL140100A0 (enExample)
NO (1) NO20006675D0 (enExample)
NZ (1) NZ508287A (enExample)
WO (1) WO2000000632A1 (enExample)
ZA (1) ZA200007261B (enExample)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
CN1230546C (zh) 1998-10-06 2005-12-07 马克·阿龙·埃马尔法尔布 丝状真菌宿主领域的转化系统
US7488590B2 (en) 1998-10-23 2009-02-10 Amgen Inc. Modified peptides as therapeutic agents
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
IL137868A0 (en) 1999-01-19 2001-10-31 Maxygen Inc Oligonucleotide mediated nucleic acid recombination
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6539102B1 (en) 2000-09-01 2003-03-25 Large Scale Proteomics Reference database
US6980674B2 (en) 2000-09-01 2005-12-27 Large Scale Proteomics Corp. Reference database
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
EP1341911A1 (en) * 2000-12-06 2003-09-10 Novozymes A/S Method for producing a polynucleotide library
US6689568B2 (en) 2001-02-01 2004-02-10 Agilent Technologies, Inc. Capture arrays using polypeptide capture agents
ATE447019T1 (de) * 2001-02-02 2009-11-15 Large Scale Biology Corp Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid
EP1470219A4 (en) 2001-04-16 2005-10-05 California Inst Of Techn PEROXIDE MOLDED CYTOCHROME OXYGENASE P450 OXYGENASE VARIANTS
US20030032028A1 (en) * 2001-06-12 2003-02-13 Gayle Dace In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads
WO2003008563A2 (en) 2001-07-20 2003-01-30 California Institute Of Technology Improved cytochrome p450 oxygenases
WO2003062417A1 (fr) * 2002-01-22 2003-07-31 Mitsubishi Chemical Corporation Produit de ligation arn-adn, et utilisation correspondante
ES2564570T3 (es) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Métodos, sistemas y software para la identificación de biomoléculas funcionales
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
BRPI0309391B1 (pt) 2002-04-19 2018-10-30 Dsm Food Specialties B V vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
MXPA05001287A (es) 2002-08-06 2005-08-03 Verdia Llc Variantes de amino oxidasa ap1.
PL3023498T3 (pl) 2003-03-06 2019-05-31 Basf Enzymes Llc Amylazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i zastosowania
AU2004277368B2 (en) 2003-03-07 2011-01-20 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and mehods for making and using them
WO2004090099A2 (en) 2003-04-04 2004-10-21 Diversa Corporation Pectate lyases, nucleic acids encoding them and methods for making and using them
CA2521284C (en) 2003-04-29 2014-07-08 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
DK1641910T3 (da) 2003-07-02 2013-05-21 Verenium Corp Glucanaser, nucleinsyrer, som koder for disse, og fremgangsmåder til at fremstille og benytte disse
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
DK1660646T3 (en) 2003-08-11 2015-03-09 California Inst Of Techn Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
JP2005065575A (ja) * 2003-08-22 2005-03-17 Japan Science & Technology Agency フレームシャッフリングによるタンパク質分子多様性集団の作製
CA2570872A1 (en) 2004-06-16 2006-01-26 Diversa Corporation Compositions and methods for enzymatic decolorization of chlorophyll
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
KR101393679B1 (ko) 2005-03-15 2014-05-21 비피 코포레이션 노쓰 아메리카 인코포레이티드 셀룰라제, 이것을 암호화하는 핵산 및 이들을 제조 및사용하는 방법
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
AU2006338208B2 (en) 2006-02-10 2013-10-24 Bp Corporation North America Inc. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP2548954A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
CN101437954B (zh) 2006-03-07 2015-09-09 嘉吉公司 醛缩酶和编码醛缩酶的核酸以及它们的制备和使用方法
KR101744157B1 (ko) 2006-03-07 2017-06-08 바스프 엔자임스 엘엘씨 알돌라제, 이것을 코딩하는 핵산과 그 제조 방법 및 사용 방법
DK2069389T3 (en) 2006-08-04 2015-01-12 Bp Corp North America Inc Glucanases, nucleic acids encoding them, and processes for their preparation and use
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2617815B1 (en) 2006-09-21 2015-11-18 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
ES2451266T3 (es) 2006-09-21 2014-03-26 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
EP2505651A3 (en) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
ES2531167T5 (es) 2006-12-21 2018-05-21 Basf Enzymes Llc Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas
EP2074136A4 (en) 2007-01-30 2012-11-07 Verenium Corp ENZYMES FOR TREATING LIGNOCELLULOSE, FOR SUCH CODING NUCLEIC ACIDS, AND METHOD FOR THE PRODUCTION AND USE THEREOF
EP2197893B1 (en) 2007-09-07 2013-07-24 Dyadic International, Inc. Novel fungal enzymes
MX2010003600A (es) 2007-10-03 2010-12-14 Verenium Corp Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
DK2238242T5 (en) 2008-01-03 2015-08-03 Basf Enzymes Llc -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF
DK2238261T3 (da) 2008-01-03 2014-02-10 Verenium Corp Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf
EP2297310A2 (en) 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
EP3502256A3 (en) 2008-09-26 2019-09-25 Tocagen Inc. Recombinant vectors
WO2010135588A2 (en) 2009-05-21 2010-11-25 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
BR112013003224A2 (pt) 2010-08-13 2016-06-07 Pioneer Hi Bred Int "construto de promotor quimérico, cassete de expressão, vetor de expressão, método de obtenção de uma planta, método para regular a expressão de um polinucleotídeo de interesse, polinucleotídeo, método para expressar um polinucleotídeo de interesse"
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
JP6004423B2 (ja) * 2012-05-07 2016-10-05 国立研究開発法人産業技術総合研究所 遺伝子連結法およびそれを用いた単鎖抗体作製方法
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
US10023877B2 (en) 2013-03-15 2018-07-17 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
CN106232820A (zh) 2013-08-16 2016-12-14 先锋国际良种公司 杀昆虫蛋白及其使用方法
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
CN114763376A (zh) 2014-02-07 2022-07-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
PH12016501534B1 (en) 2014-02-07 2024-06-05 Du Pont Insecticidal proteins and methods for their use
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2016138292A1 (en) * 2015-02-25 2016-09-01 Igenomx International Genomics Corporation Methods and compositions for in silico long read sequencing
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX387506B (es) 2016-07-01 2025-03-18 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
WO2018208882A1 (en) 2017-05-11 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110158157B (zh) * 2018-02-13 2021-02-02 浙江大学 基于模板材料合成长度固定和特定末端序列dna文库的方法
EP3764798B1 (en) 2018-03-14 2025-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11820791B2 (en) 2018-03-14 2023-11-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2020198731A2 (en) 2019-03-28 2020-10-01 Danisco Us Inc Engineered antibodies
WO2021142393A1 (en) 2020-01-10 2021-07-15 The Regents Of The University Of California Biosynthetic platform for the production of olivetolic avid and analogues of olivetolic acid
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) * 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US5447839A (en) * 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5580730A (en) * 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
CA2240346C (en) * 1995-12-15 2007-04-24 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
NZ337900A (en) * 1997-03-18 2001-05-25 Novo Nordisk As DNA shuffling for randomised heterologous and homogenous 3' and 5' primers for construction of DNA libraries for generation of more desirable mutants
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers

Also Published As

Publication number Publication date
ZA200007261B (en) 2002-05-15
CN1308683A (zh) 2001-08-15
AU761570B2 (en) 2003-06-05
NZ508287A (en) 2003-08-29
ES2381824T3 (es) 2012-05-31
WO2000000632A1 (en) 2000-01-06
EP1092039B1 (en) 2012-02-29
EP1092039A1 (en) 2001-04-18
WO2000000632A9 (en) 2000-10-26
JP2002519038A (ja) 2002-07-02
ATE547532T1 (de) 2012-03-15
IL140100A0 (en) 2002-02-10
JP4700805B2 (ja) 2011-06-15
AU4847099A (en) 2000-01-17
KR20010071613A (ko) 2001-07-28
NO20006675L (no) 2000-12-28
EP1092039A4 (en) 2003-07-02
CA2331926C (en) 2013-09-10
CA2331926A1 (en) 2000-01-06

Similar Documents

Publication Publication Date Title
NO20006675L (no) Fremgangsmåte for fremstilling av höyt varierte bibliotek
JP6894501B2 (ja) 等温核酸自己増幅法
AU672969B2 (en) Exchangeable template reaction
JP2002519038A5 (enExample)
Cukras et al. Chess games: a model for RNA based computation
WO1997000330A3 (de) Verfahren zur transkriptionsfreien amplifizierung von nukleinsäuren
DE50114507D1 (de) Nukleinsäure-Linker und deren Verwendung in der Gensynthese
JP2005503802A5 (enExample)
HUT52823A (en) Process for producing transcription-based, nucleinic acid multiplying/detecting systems
DE10081549D2 (de) Verfahren zur Synthese von DNA-Fragmenten
EP3322712A1 (en) Azidomethyl ether deprotection method
PT1056884E (pt) Processo para a amplificacao nao especifica de acido nucleico
EP0359545A3 (en) Method of making double-stranded dna sequences
ATE431407T1 (de) Verfahren zur herstellung von dna
WO2003072798A3 (en) Method for generating amplified rna
ATE307901T1 (de) Amplifikation und sequenzierung von primer-paaren und deren verwendung
CA2264967A1 (en) Method of dna sequencing
EP3704248B1 (en) Methods and kits for targeted enrichment of target dna with high gc content
Lindström et al. An orthogonal oligonucleotide protecting group strategy that enables assembly of repetitive or highly structured DNAs
EP1333089A4 (en) SYNTHESIS METHOD FOR SINGLE STRANDED NUCLEIC ACID
ATE223499T1 (de) Von kreisförmiger matrize abhängige kettenreaktion
Greenwood et al. Molecular characterization of U3 small nucleolar RNA from the early diverging protist, Euglena gracilis
CN109306376B (zh) 检验核酸扩增均一性的质量控制标准品及其制备方法
JP2000353156A (ja) Np完全問題の解を得る方法
WO2002050094A3 (en) Nucleic acid synthesis by primer extension on successive templates

Legal Events

Date Code Title Description
FC2A Withdrawal, rejection or dismissal of laid open patent application