JP4700805B2 - 多様性の高いライブラリーを製造する方法 - Google Patents
多様性の高いライブラリーを製造する方法 Download PDFInfo
- Publication number
- JP4700805B2 JP4700805B2 JP2000557385A JP2000557385A JP4700805B2 JP 4700805 B2 JP4700805 B2 JP 4700805B2 JP 2000557385 A JP2000557385 A JP 2000557385A JP 2000557385 A JP2000557385 A JP 2000557385A JP 4700805 B2 JP4700805 B2 JP 4700805B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- stranded nucleic
- population
- strand
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Compounds Of Unknown Constitution (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9097098P | 1998-06-29 | 1998-06-29 | |
| US60/090,970 | 1998-06-29 | ||
| PCT/US1999/014776 WO2000000632A1 (en) | 1998-06-29 | 1999-06-29 | Methods for generating highly diverse libraries |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002519038A JP2002519038A (ja) | 2002-07-02 |
| JP2002519038A5 JP2002519038A5 (enExample) | 2006-08-31 |
| JP4700805B2 true JP4700805B2 (ja) | 2011-06-15 |
Family
ID=22225171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000557385A Expired - Lifetime JP4700805B2 (ja) | 1998-06-29 | 1999-06-29 | 多様性の高いライブラリーを製造する方法 |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1092039B1 (enExample) |
| JP (1) | JP4700805B2 (enExample) |
| KR (1) | KR20010071613A (enExample) |
| CN (1) | CN1308683A (enExample) |
| AT (1) | ATE547532T1 (enExample) |
| AU (1) | AU761570B2 (enExample) |
| CA (1) | CA2331926C (enExample) |
| ES (1) | ES2381824T3 (enExample) |
| IL (1) | IL140100A0 (enExample) |
| NO (1) | NO20006675D0 (enExample) |
| NZ (1) | NZ508287A (enExample) |
| WO (1) | WO2000000632A1 (enExample) |
| ZA (1) | ZA200007261B (enExample) |
Families Citing this family (103)
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| US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
| CN1230546C (zh) | 1998-10-06 | 2005-12-07 | 马克·阿龙·埃马尔法尔布 | 丝状真菌宿主领域的转化系统 |
| US7488590B2 (en) | 1998-10-23 | 2009-02-10 | Amgen Inc. | Modified peptides as therapeutic agents |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US8457903B1 (en) | 1999-01-19 | 2013-06-04 | Codexis Mayflower Holdings, Llc | Method and/or apparatus for determining codons |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US7873477B1 (en) | 2001-08-21 | 2011-01-18 | Codexis Mayflower Holdings, Llc | Method and system using systematically varied data libraries |
| AU3210100A (en) | 1999-01-19 | 2000-08-01 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| WO2001032712A2 (en) | 1999-11-03 | 2001-05-10 | Maxygen, Inc. | Antibody diversity generation |
| US7115403B1 (en) | 2000-05-16 | 2006-10-03 | The California Institute Of Technology | Directed evolution of galactose oxidase enzymes |
| US6539102B1 (en) | 2000-09-01 | 2003-03-25 | Large Scale Proteomics | Reference database |
| US6980674B2 (en) | 2000-09-01 | 2005-12-27 | Large Scale Proteomics Corp. | Reference database |
| US6783941B2 (en) | 2000-12-06 | 2004-08-31 | Novozymes A/S | Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes |
| AU2002220529A1 (en) * | 2000-12-06 | 2002-06-18 | Novozymes A/S | Method for producing a polynucleotide library |
| US6689568B2 (en) | 2001-02-01 | 2004-02-10 | Agilent Technologies, Inc. | Capture arrays using polypeptide capture agents |
| CA2436214C (en) * | 2001-02-02 | 2012-12-04 | Large Scale Biology Corporation | A method of increasing complementarity in a heteroduplex polynucleotide |
| WO2002083868A2 (en) | 2001-04-16 | 2002-10-24 | California Institute Of Technology | Peroxide-driven cytochrome p450 oxygenase variants |
| US20030032028A1 (en) * | 2001-06-12 | 2003-02-13 | Gayle Dace | In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads |
| WO2003008563A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Improved cytochrome p450 oxygenases |
| WO2003062417A1 (fr) * | 2002-01-22 | 2003-07-31 | Mitsubishi Chemical Corporation | Produit de ligation arn-adn, et utilisation correspondante |
| EP1493027B1 (en) | 2002-03-01 | 2014-10-15 | Codexis Mayflower Holdings, LLC | Methods, systems, and software for identifying functional bio-molecules |
| JP4851687B2 (ja) | 2002-03-09 | 2012-01-11 | マキシジェン, インコーポレイテッド | 定向進化のための交叉点の最適化 |
| CN102174546A (zh) | 2002-04-19 | 2011-09-07 | 维莱尼姆公司 | 磷脂酶,编码磷脂酶的核酸以及制备和应用磷脂酶的方法 |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| EP2275536A1 (en) | 2002-08-06 | 2011-01-19 | Verdia, Inc. | AP1 amine oxidase variants |
| CN102618564B (zh) | 2003-03-06 | 2014-10-29 | 维莱尼姆公司 | 淀粉酶、编码它们的核酸及其制备和应用方法 |
| DK2853593T3 (en) | 2003-03-07 | 2018-01-08 | Dsm Ip Assets Bv | Hydrolases, nucleic acids encoding them, and processes for their preparation and use |
| PL1613733T3 (pl) | 2003-04-04 | 2015-11-30 | Basf Enzymes Llc | Liazy pektynianowe, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i zastosowania |
| EP1620557A2 (en) | 2003-04-29 | 2006-02-01 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
| EP1639091B1 (en) | 2003-06-17 | 2012-12-05 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| CN101370819A (zh) | 2003-07-02 | 2009-02-18 | 维莱尼姆公司 | 葡聚糖酶,编码它们的核酸以及制备和使用它们的方法 |
| US7741089B2 (en) | 2003-08-11 | 2010-06-22 | Verenium Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
| US7435570B2 (en) | 2003-08-11 | 2008-10-14 | California Institute Of Technology | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
| JP2005065575A (ja) * | 2003-08-22 | 2005-03-17 | Japan Science & Technology Agency | フレームシャッフリングによるタンパク質分子多様性集団の作製 |
| AU2005264938B2 (en) | 2004-06-16 | 2011-11-24 | Verenium Corporation | Compositions and methods for enzymatic decolorization of chlorophyll |
| US20090038023A1 (en) | 2005-03-10 | 2009-02-05 | Verenium Corporation | Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them |
| DK1861506T3 (en) | 2005-03-15 | 2015-07-20 | Bp Corp North America Inc | Cellulases, nucleic acids encoding them, and methods of making and using them |
| US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US20090324574A1 (en) | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
| DK1989301T3 (en) | 2006-02-10 | 2015-01-05 | Bp Corp North America Inc | Cellulolytic enzymes, nucleic acids encoding them, and methods for making and using them |
| DK1989302T3 (en) | 2006-02-14 | 2018-07-23 | Bp Corp North America Inc | XYLANASES, NUCLEIC ACID CODING THEM AND PROCEDURES FOR THEIR PREPARATION AND USE |
| ES2416104T3 (es) | 2006-03-07 | 2013-07-30 | Cargill, Incorporated | Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas |
| AU2007223086B2 (en) | 2006-03-07 | 2013-09-26 | Basf Enzymes Llc | Aldolases, nucleic acids encoding them and methods for making and using them |
| EP2069389B1 (en) | 2006-08-04 | 2014-10-08 | BP Corporation North America Inc. | Glucanases, nucleic acids encoding them and methods for making and using them |
| US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| WO2008016709A2 (en) | 2006-08-04 | 2008-02-07 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| DK2057178T3 (da) | 2006-09-21 | 2014-02-03 | Verenium Corp | Phytaser, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og benytte dem |
| EA015591B1 (ru) | 2006-09-21 | 2011-10-31 | Верениум Корпорейшн | Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения |
| EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
| HUE033455T2 (en) | 2006-12-21 | 2017-12-28 | Basf Enzymes Llc | Amylases and glucoamylases, nucleic acids encoding them, and methods for their preparation and use |
| AU2008210495B2 (en) | 2007-01-30 | 2014-02-27 | Bp Corporation North America, Inc. | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
| WO2009033071A2 (en) | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
| USRE46733E1 (en) | 2007-10-03 | 2018-02-27 | Bp Corporation North America Inc. | Xylanases, nucleic acids encoding them and methods for making and using them |
| CA2710683A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
| CA2710922A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Isomerases, nucleic acids encoding them and methods for making and using them |
| WO2009143397A2 (en) | 2008-05-23 | 2009-11-26 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
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| UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
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| US9187762B2 (en) | 2010-08-13 | 2015-11-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity |
| WO2013016207A2 (en) | 2011-07-22 | 2013-01-31 | California Institute Of Technology | Stable fungal cel6 enzyme variants |
| BR112014027468A2 (pt) | 2012-05-04 | 2017-06-27 | Du Pont | polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo. |
| JP6004423B2 (ja) * | 2012-05-07 | 2016-10-05 | 国立研究開発法人産業技術総合研究所 | 遺伝子連結法およびそれを用いた単鎖抗体作製方法 |
| EP2970995A1 (en) | 2013-03-14 | 2016-01-20 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
| BR112015023286A2 (pt) | 2013-03-14 | 2018-03-06 | Arzeda Corp | polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase |
| CA2901316A1 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
| EA030896B1 (ru) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| CA3223359A1 (en) | 2013-09-13 | 2015-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3087088B1 (en) | 2013-12-23 | 2022-05-25 | University Of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
| RU2021113662A (ru) | 2014-02-07 | 2021-05-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| CA2938979C (en) | 2014-02-07 | 2024-02-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| MX381370B (es) | 2014-10-16 | 2025-03-12 | Corteva Agriscience Llc | Proteinas insecticidas y metodos de uso. |
| RU2740312C2 (ru) | 2015-01-15 | 2021-01-13 | Пайониэ Хай-Бред Интернешнл, Инк. | Инсектицидные белки и способы их применения |
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| CA2977026A1 (en) | 2015-03-11 | 2016-09-15 | E.I. Du Pont De Nemours And Company | Insecticidal combinations of pip-72 and methods of use |
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| WO2017023486A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
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| US11339427B2 (en) | 2016-02-12 | 2022-05-24 | Jumpcode Genomics, Inc. | Method for target specific RNA transcription of DNA sequences |
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| EP3535285B1 (en) | 2016-11-01 | 2022-04-06 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| BR112019012339A2 (pt) | 2016-12-14 | 2019-11-26 | Pioneer Hi Bred Int | polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão |
| MX2019007491A (es) | 2016-12-22 | 2019-09-06 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
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| CN110158157B (zh) * | 2018-02-13 | 2021-02-02 | 浙江大学 | 基于模板材料合成长度固定和特定末端序列dna文库的方法 |
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| US20230051453A1 (en) | 2020-01-10 | 2023-02-16 | The Regents Of The University Of California | Biosynthetic platform for the production of olivetolic acid and analogues of olivetolic acid |
| WO2022015619A2 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
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| US4994379A (en) * | 1983-03-09 | 1991-02-19 | Cetus Corporation | Modified signal peptides |
| US5447839A (en) * | 1988-09-09 | 1995-09-05 | Hoffmann-La Roche Inc. | Detection of human papillomavirus by the polymerase chain reaction |
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| US5922539A (en) * | 1995-12-15 | 1999-07-13 | Duke University | Methods for use of mismatch repair systems for the detection and removal of mutant sequences that arise during enzymatic amplification |
| DE69839668D1 (de) * | 1997-03-18 | 2008-08-14 | Novozymes As | Ein in-vitro-verfahren zur herstellung einer dna-bibliothek |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
-
1999
- 1999-06-29 KR KR1020007014838A patent/KR20010071613A/ko not_active Withdrawn
- 1999-06-29 WO PCT/US1999/014776 patent/WO2000000632A1/en not_active Ceased
- 1999-06-29 AU AU48470/99A patent/AU761570B2/en not_active Expired
- 1999-06-29 CN CN99807977A patent/CN1308683A/zh active Pending
- 1999-06-29 AT AT99932081T patent/ATE547532T1/de active
- 1999-06-29 JP JP2000557385A patent/JP4700805B2/ja not_active Expired - Lifetime
- 1999-06-29 ES ES99932081T patent/ES2381824T3/es not_active Expired - Lifetime
- 1999-06-29 CA CA2331926A patent/CA2331926C/en not_active Expired - Lifetime
- 1999-06-29 IL IL14010099A patent/IL140100A0/xx unknown
- 1999-06-29 NZ NZ508287A patent/NZ508287A/en not_active IP Right Cessation
- 1999-06-29 EP EP99932081A patent/EP1092039B1/en not_active Expired - Lifetime
-
2000
- 2000-12-07 ZA ZA200007261A patent/ZA200007261B/en unknown
- 2000-12-28 NO NO20006675A patent/NO20006675D0/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU761570B2 (en) | 2003-06-05 |
| NZ508287A (en) | 2003-08-29 |
| CA2331926A1 (en) | 2000-01-06 |
| WO2000000632A1 (en) | 2000-01-06 |
| WO2000000632A9 (en) | 2000-10-26 |
| EP1092039A1 (en) | 2001-04-18 |
| ES2381824T3 (es) | 2012-05-31 |
| CA2331926C (en) | 2013-09-10 |
| IL140100A0 (en) | 2002-02-10 |
| ATE547532T1 (de) | 2012-03-15 |
| EP1092039B1 (en) | 2012-02-29 |
| NO20006675L (no) | 2000-12-28 |
| JP2002519038A (ja) | 2002-07-02 |
| KR20010071613A (ko) | 2001-07-28 |
| EP1092039A4 (en) | 2003-07-02 |
| ZA200007261B (en) | 2002-05-15 |
| AU4847099A (en) | 2000-01-17 |
| NO20006675D0 (no) | 2000-12-28 |
| CN1308683A (zh) | 2001-08-15 |
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