ATE447019T1 - Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid - Google Patents

Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid

Info

Publication number
ATE447019T1
ATE447019T1 AT02741635T AT02741635T ATE447019T1 AT E447019 T1 ATE447019 T1 AT E447019T1 AT 02741635 T AT02741635 T AT 02741635T AT 02741635 T AT02741635 T AT 02741635T AT E447019 T1 ATE447019 T1 AT E447019T1
Authority
AT
Austria
Prior art keywords
heteroduplex
complementarity
polynucleotide
polynucleotides
mismatches
Prior art date
Application number
AT02741635T
Other languages
English (en)
Inventor
Hal Padgett
Wayne Fitzmaurice
John Lindbo
Original Assignee
Large Scale Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp filed Critical Large Scale Biology Corp
Application granted granted Critical
Publication of ATE447019T1 publication Critical patent/ATE447019T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT02741635T 2001-02-02 2002-02-01 Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid ATE447019T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26638601P 2001-02-02 2001-02-02
US26878501P 2001-02-14 2001-02-14
PCT/US2002/003055 WO2002079468A2 (en) 2001-02-02 2002-02-01 A method of increasing complementarity in a heteroduplex polynucleotide

Publications (1)

Publication Number Publication Date
ATE447019T1 true ATE447019T1 (de) 2009-11-15

Family

ID=26951797

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02741635T ATE447019T1 (de) 2001-02-02 2002-02-01 Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid

Country Status (8)

Country Link
US (4) US20020146732A1 (de)
EP (1) EP1358322B1 (de)
JP (1) JP4689940B2 (de)
AT (1) ATE447019T1 (de)
AU (1) AU2002314712C1 (de)
CA (1) CA2436214C (de)
DE (1) DE60234159D1 (de)
WO (1) WO2002079468A2 (de)

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WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
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EP3359669B1 (de) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Vorrichtungen und verfahren zur herstellung von proteinen
EP3814494B1 (de) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH Hochdurchsatzanordnung von nukleinsäuremolekülen
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2021178809A1 (en) 2020-03-06 2021-09-10 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
EP4638776A1 (de) 2022-12-19 2025-10-29 Thermo Fisher Scientific GENEART GmbH Rückgewinnung von sequenzverifizierten nukleinsäuremolekülen

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US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
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Also Published As

Publication number Publication date
WO2002079468A8 (en) 2003-12-04
WO2002079468A3 (en) 2003-03-27
US7217514B2 (en) 2007-05-15
US20020177160A1 (en) 2002-11-28
US7235386B2 (en) 2007-06-26
CA2436214C (en) 2012-12-04
WO2002079468A2 (en) 2002-10-10
JP2005507641A (ja) 2005-03-24
EP1358322B1 (de) 2009-10-28
AU2002314712B2 (en) 2008-03-06
EP1358322A2 (de) 2003-11-05
US20080032346A1 (en) 2008-02-07
DE60234159D1 (de) 2009-12-10
US7833759B2 (en) 2010-11-16
AU2002314712C1 (en) 2008-10-02
US20020146732A1 (en) 2002-10-10
JP4689940B2 (ja) 2011-06-01
US20030186261A1 (en) 2003-10-02
CA2436214A1 (en) 2002-10-10

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