ATE366807T1 - Fehlpaarungsendonukleasen und verwendungsverfahren - Google Patents

Fehlpaarungsendonukleasen und verwendungsverfahren

Info

Publication number
ATE366807T1
ATE366807T1 AT03706015T AT03706015T ATE366807T1 AT E366807 T1 ATE366807 T1 AT E366807T1 AT 03706015 T AT03706015 T AT 03706015T AT 03706015 T AT03706015 T AT 03706015T AT E366807 T1 ATE366807 T1 AT E366807T1
Authority
AT
Austria
Prior art keywords
heteroduplex
complementarity
polynucleotide
mismatches
increased
Prior art date
Application number
AT03706015T
Other languages
English (en)
Inventor
Hal Padgett
Andrew Vaewhongs
Fakhrieh Vojdani
Mark Smith
John Lindbo
Wayne Fitzmaurice
Original Assignee
Large Scale Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/098,155 external-priority patent/US20030157495A1/en
Application filed by Large Scale Biology Corp filed Critical Large Scale Biology Corp
Application granted granted Critical
Publication of ATE366807T1 publication Critical patent/ATE366807T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Disintegrating Or Milling (AREA)
AT03706015T 2002-02-01 2003-01-31 Fehlpaarungsendonukleasen und verwendungsverfahren ATE366807T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35372202P 2002-02-01 2002-02-01
US10/098,155 US20030157495A1 (en) 2002-02-01 2002-03-14 Nucleic acid molecules encoding CEL I endonuclease and methods of use thereof
US10/211,079 US7078211B2 (en) 2002-02-01 2002-08-01 Nucleic acid molecules encoding endonucleases and methods of use thereof

Publications (1)

Publication Number Publication Date
ATE366807T1 true ATE366807T1 (de) 2007-08-15

Family

ID=27739010

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03706015T ATE366807T1 (de) 2002-02-01 2003-01-31 Fehlpaarungsendonukleasen und verwendungsverfahren

Country Status (8)

Country Link
US (4) US7078211B2 (de)
EP (1) EP1565557B1 (de)
JP (1) JP4395632B2 (de)
AT (1) ATE366807T1 (de)
AU (1) AU2003207776B2 (de)
CA (1) CA2473187C (de)
DE (1) DE60314902T2 (de)
WO (1) WO2003066809A2 (de)

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US20110111413A1 (en) * 2001-02-02 2011-05-12 Padgett Hal S Method of optimizing codon usage through dna shuffling
ATE447019T1 (de) * 2001-02-02 2009-11-15 Large Scale Biology Corp Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
EP1574570A1 (de) * 2004-03-12 2005-09-14 Universität Regensburg Verfahren zur Verringerung der Anzahl von fehlgepaarten Nukleotiden in doppelsträngigen Polynukleotiden
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
US20060029612A1 (en) * 2004-04-15 2006-02-09 Large Scale Biology Corporation Prevention and treatment of recurrent respiratory papillomatosis
US7729761B2 (en) * 2004-07-14 2010-06-01 Cardiac Pacemakers, Inc. Method and apparatus for controlled gene or protein delivery
ATE495243T1 (de) * 2004-07-30 2011-01-15 Genoplante Valor Verfahren zur herstellung von hochempfindlichen endonukleasen, neuartige zubereitungen von nukleasen und ihre verwendung
EP2484768A3 (de) * 2005-07-18 2012-11-21 Protalix Ltd. Mukosale oder enterale Verabreichung biologisch aktiver Makromoleküle
EP1934335A4 (de) * 2005-09-08 2010-05-05 Large Scale Biology Corp Modifizierte tabak-mosaikvirus-teilchen als gerüste zur anzeige von protein-antigenen für impfstoff-anwendungen
WO2007101028A2 (en) * 2006-02-24 2007-09-07 Transgenomic, Inc. Method to produce enzymatically active recombinant endonucleases
US7888475B2 (en) * 2008-09-15 2011-02-15 Padgett Hal S Interferon alpha and interferon kappa fusion protein
WO2011102802A1 (en) * 2010-02-18 2011-08-25 Agency For Science, Technology And Research Method for reducing mismatches in double-stranded dna molecules
EP2768607B1 (de) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell-platte für die hocheffiziente kleinvolumige nukleinsäuresynthese
WO2013116771A1 (en) * 2012-02-01 2013-08-08 Synthetic Genomics, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
US20130274129A1 (en) 2012-04-04 2013-10-17 Geneart Ag Tal-effector assembly platform, customized services, kits and assays
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20150119260A1 (en) * 2013-10-18 2015-04-30 National Taiwan University Circulating cancer biomarker and its use
EP3169781B1 (de) 2014-07-15 2020-04-08 Life Technologies Corporation Zusammensetzungen und verfahren zur nukleinsäureassemblierung
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
WO2017007925A1 (en) 2015-07-07 2017-01-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
EP3763818A1 (de) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Vorrichtungen und verfahren zur herstellung von proteinen
WO2017125893A1 (en) * 2016-01-22 2017-07-27 Vyome Biosciences Pvt. Ltd. Method of detecting and treating p. acnes and kit thereof
WO2020001783A1 (en) 2018-06-29 2020-01-02 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
JP2023516827A (ja) 2020-03-06 2023-04-20 ライフ テクノロジーズ コーポレイション 高配列忠実度の核酸合成およびアセンブリ
US11667968B2 (en) 2021-05-27 2023-06-06 New England Biolabs, Inc. Fragmentation of DNA
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

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US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
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Also Published As

Publication number Publication date
US7056740B2 (en) 2006-06-06
US20080145913A1 (en) 2008-06-19
US7078211B2 (en) 2006-07-18
DE60314902D1 (de) 2007-08-23
US7273739B2 (en) 2007-09-25
AU2003207776B2 (en) 2008-12-11
AU2003207776A1 (en) 2003-09-02
DE60314902T2 (de) 2008-04-10
EP1565557A4 (de) 2005-11-30
JP2005529586A (ja) 2005-10-06
WO2003066809A2 (en) 2003-08-14
US20030157682A1 (en) 2003-08-21
JP4395632B2 (ja) 2010-01-13
CA2473187A1 (en) 2003-08-14
US20060194288A1 (en) 2006-08-31
CA2473187C (en) 2012-07-31
WO2003066809A3 (en) 2005-06-30
US20030148315A1 (en) 2003-08-07
EP1565557B1 (de) 2007-07-11
EP1565557A2 (de) 2005-08-24

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