ATE513911T1 - Verfahren und zusammensetzungen für rna- interferenz - Google Patents
Verfahren und zusammensetzungen für rna- interferenzInfo
- Publication number
- ATE513911T1 ATE513911T1 AT03732052T AT03732052T ATE513911T1 AT E513911 T1 ATE513911 T1 AT E513911T1 AT 03732052 T AT03732052 T AT 03732052T AT 03732052 T AT03732052 T AT 03732052T AT E513911 T1 ATE513911 T1 AT E513911T1
- Authority
- AT
- Austria
- Prior art keywords
- gene
- compositions
- methods
- rna interference
- cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 title 1
- 230000009368 gene silencing by RNA Effects 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
- C12Y301/26003—Ribonuclease III (3.1.26.3)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Oncology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Communicable Diseases (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/055,797 US20030084471A1 (en) | 2000-03-16 | 2002-01-22 | Methods and compositions for RNA interference |
PCT/US2003/001963 WO2003062394A2 (en) | 2002-01-22 | 2003-01-22 | Methods and compositions for rna interference |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE513911T1 true ATE513911T1 (de) | 2011-07-15 |
Family
ID=27609226
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03732052T ATE513911T1 (de) | 2002-01-22 | 2003-01-22 | Verfahren und zusammensetzungen für rna- interferenz |
Country Status (10)
Country | Link |
---|---|
US (1) | US20030084471A1 (de) |
EP (1) | EP1546174B1 (de) |
JP (2) | JP4758067B2 (de) |
AT (1) | ATE513911T1 (de) |
CA (1) | CA2473944A1 (de) |
DK (1) | DK1546174T3 (de) |
ES (1) | ES2368039T3 (de) |
HK (1) | HK1073660A1 (de) |
SI (1) | SI1546174T1 (de) |
WO (1) | WO2003062394A2 (de) |
Families Citing this family (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6939712B1 (en) * | 1998-12-29 | 2005-09-06 | Impedagen, Llc | Muting gene activity using a transgenic nucleic acid |
EP1147204A1 (de) * | 1999-01-28 | 2001-10-24 | Medical College Of Georgia Research Institute, Inc. | Zusammensetzung und verfahren zur in vivo und in vitro abschwächung der genexpression mittels dobbelsträngigem rna |
WO2000063364A2 (en) * | 1999-04-21 | 2000-10-26 | American Home Products Corporation | Methods and compositions for inhibiting the function of polynucleotide sequences |
US20040138168A1 (en) * | 1999-04-21 | 2004-07-15 | Wyeth | Methods and compositions for inhibiting the function of polynucleotide sequences |
GB9925459D0 (en) * | 1999-10-27 | 1999-12-29 | Plant Bioscience Ltd | Gene silencing |
US20030181412A1 (en) * | 1999-12-21 | 2003-09-25 | Ingeneus Corporation | Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses |
US8202846B2 (en) * | 2000-03-16 | 2012-06-19 | Cold Spring Harbor Laboratory | Methods and compositions for RNA interference |
US20030084471A1 (en) * | 2000-03-16 | 2003-05-01 | David Beach | Methods and compositions for RNA interference |
EP1272630A2 (de) * | 2000-03-16 | 2003-01-08 | Genetica, Inc. | Methoden und zusammensetzungen zur interferenz durch rna |
AU2001249622B2 (en) | 2000-03-30 | 2007-06-07 | Massachusetts Institute Of Technology | RNA sequence-specific mediators of RNA interference |
HU230458B1 (hu) | 2000-12-01 | 2016-07-28 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Az RNS interferenciát közvetítő kis RNS molekulák |
ES2566561T3 (es) * | 2001-07-12 | 2016-04-13 | University Of Massachusetts | Producción in vivo de ARN pequeños de interferencia que median el silenciamiento génico |
PT2280070E (pt) | 2001-07-23 | 2015-10-29 | Univ Leland Stanford Junior | Métodos e composições para inibição mediada por iarn da expressão génica em mamíferos |
US10590418B2 (en) * | 2001-07-23 | 2020-03-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for RNAi mediated inhibition of gene expression in mammals |
US7737124B2 (en) | 2001-09-13 | 2010-06-15 | California Institute Of Technology | Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell |
US7919309B2 (en) * | 2001-09-13 | 2011-04-05 | California Institute Of Technology | Method for expression of small antiviral RNA molecules within a cell |
WO2003046173A1 (fr) * | 2001-11-28 | 2003-06-05 | Center For Advanced Science And Technology Incubation, Ltd. | Systeme d'expression d'arn si et procede de production de cellules d'inactivation de genes fonctionnelles et analogues au moyen de ce systeme |
WO2003064625A2 (en) | 2002-02-01 | 2003-08-07 | Sequitur, Inc. | Oligonucleotide compositions with enhanced efficiency |
US20030166282A1 (en) | 2002-02-01 | 2003-09-04 | David Brown | High potency siRNAS for reducing the expression of target genes |
US20060009409A1 (en) | 2002-02-01 | 2006-01-12 | Woolf Tod M | Double-stranded oligonucleotides |
EP1483281B2 (de) * | 2002-02-14 | 2012-09-05 | City of Hope | Verfahren zur herstellung interferierender rna-moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle |
EP1572923A4 (de) * | 2002-03-06 | 2007-10-31 | Rigel Pharmaceuticals Inc | Neues verfahren zur zuführung und intrazellulären synthese von sirna-molekülen |
US7274703B2 (en) * | 2002-03-11 | 2007-09-25 | 3Com Corporation | Stackable network units with resiliency facility |
US20030180756A1 (en) * | 2002-03-21 | 2003-09-25 | Yang Shi | Compositions and methods for suppressing eukaryotic gene expression |
WO2003093430A2 (en) * | 2002-05-03 | 2003-11-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in preparing sirnas |
US20040106566A1 (en) * | 2002-05-17 | 2004-06-03 | Shi-Lung Lin | RNA-splicing and processing-directed gene silencing and the relative applications thereof |
US7399586B2 (en) | 2002-05-23 | 2008-07-15 | Ceptyr, Inc. | Modulation of biological signal transduction by RNA interference |
WO2004001013A2 (en) * | 2002-06-24 | 2003-12-31 | Baylor College Of Medicine | Inhibition of gene expression in vertebrates using double-stranded rna (rnai) |
WO2004014933A1 (en) | 2002-08-07 | 2004-02-19 | University Of Massachusetts | Compositions for rna interference and methods of use thereof |
US20050196862A1 (en) * | 2002-08-30 | 2005-09-08 | Wooddell Christine I. | DNA cassette for cellular expression of small RNA |
CA2497913C (en) | 2002-09-05 | 2014-06-03 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
CA2881743A1 (en) * | 2002-09-25 | 2004-04-08 | University Of Massachusetts | In vivo gene silencing by chemically modified and stable sirna |
AU2003272147A1 (en) * | 2002-10-15 | 2004-05-04 | Phytovation B.V. | Expressional enhancers from viruses |
US20050071893A1 (en) * | 2002-10-17 | 2005-03-31 | Jost Seibler | SiRNA mediated gene silencing in transgenic animals |
EP1551971B1 (de) * | 2002-10-17 | 2008-12-24 | TaconicArtemis GmbH | Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren |
US9719094B2 (en) | 2002-11-14 | 2017-08-01 | Thermo Fisher Scientific Inc. | RNAi targeting SEC61G |
US9771586B2 (en) | 2002-11-14 | 2017-09-26 | Thermo Fisher Scientific Inc. | RNAi targeting ZNF205 |
US9879266B2 (en) | 2002-11-14 | 2018-01-30 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
WO2006006948A2 (en) | 2002-11-14 | 2006-01-19 | Dharmacon, Inc. | METHODS AND COMPOSITIONS FOR SELECTING siRNA OF IMPROVED FUNCTIONALITY |
US10011836B2 (en) | 2002-11-14 | 2018-07-03 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US9839649B2 (en) | 2002-11-14 | 2017-12-12 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US9719092B2 (en) | 2002-11-14 | 2017-08-01 | Thermo Fisher Scientific Inc. | RNAi targeting CNTD2 |
US9228186B2 (en) | 2002-11-14 | 2016-01-05 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
JP2006509504A (ja) * | 2002-12-11 | 2006-03-23 | ユニバーシティー オブ マサチューセッツ | 脂肪細胞へのsiRNAの導入方法 |
AU2004228684A1 (en) * | 2003-04-10 | 2004-10-21 | Intellectual Property Bank Corp. | Biological information monitoring system |
EP1628628A4 (de) * | 2003-05-05 | 2007-03-21 | Genzyme Corp | Verfahren zur reduzierung einer immunantwort |
US20040224405A1 (en) * | 2003-05-06 | 2004-11-11 | Dharmacon Inc. | siRNA induced systemic gene silencing in mammalian systems |
CN1780920B (zh) * | 2003-05-12 | 2012-03-28 | 波多玛克制药有限公司 | 基因表达抑制剂 |
US20060178327A1 (en) * | 2003-05-30 | 2006-08-10 | Yeung Wah Hin A | Inhibition of gene expression by delivery of specially selected double stranded or other forms of small interfering RNA precursors enabling the formation and function of small interfering RNA in vivo and in vitro |
CN101961497A (zh) | 2003-07-03 | 2011-02-02 | 宾夕法尼亚大学理事会 | 对Syk激酶表达的抑制 |
US20070202505A1 (en) * | 2003-09-08 | 2007-08-30 | Alex Chenchik | Methods for gene function analysis |
US20050053971A1 (en) * | 2003-09-08 | 2005-03-10 | Alex Chenchik | Methods and constructs for developing cell lines for biological assays |
US20050059019A1 (en) * | 2003-09-11 | 2005-03-17 | Sven Bulow | Gene-related RNAi transfection method |
DE10351149A1 (de) * | 2003-11-03 | 2005-06-30 | Beiersdorf Ag | Oligoribonukleotide zur Behandlung von unerwünschter Pigmentierung der Haut und der Haare durch RNA-Interferenz |
EP1734811A4 (de) * | 2003-11-21 | 2009-03-25 | Revivicor Inc | Verwendung von interferierender rna bei der erzeugung transgener tiere |
WO2005059120A1 (en) * | 2003-12-19 | 2005-06-30 | Stem Cell Australia Pty Lyd | Methods of preventing transplantation rejection by creating immunologically neutral stem cells using gene silencing technology |
SG188122A1 (en) * | 2004-01-23 | 2013-03-28 | Advanced Cell Tech Inc | Improved modalities for the treatment of degenerative diseases of the retina |
WO2005097817A2 (en) | 2004-04-05 | 2005-10-20 | Alnylam Pharmaceuticals, Inc. | Process and reagents for oligonucleotide synthesis and purification |
EP1586654A1 (de) * | 2004-04-15 | 2005-10-19 | Vereniging voor christelijk hoger onderwijs, wetenschappelijk onderzoek en patiëntenzorg | Replikationskompetente Viren für Genabschaltung von Virusinaktivierungsfaktor |
US20050277139A1 (en) * | 2004-04-26 | 2005-12-15 | Itzhak Bentwich | Methods and apparatus for the detection and validation of microRNAs |
JP4584986B2 (ja) * | 2004-04-27 | 2010-11-24 | アルニラム ファーマスーティカルズ インコーポレイテッド | 2−アリールプロピル部分を含む1本鎖及び2本鎖オリゴヌクレオチド |
EP1750776A2 (de) * | 2004-04-30 | 2007-02-14 | Alnylam Pharmaceuticals Inc. | Oligonukleotide mit c5-modifiziertem pyrimidin |
WO2005108572A1 (de) * | 2004-05-06 | 2005-11-17 | Medizinische Hochschule Hannover | Verbindungen und verfahren zur immunsuppression |
US8236771B2 (en) * | 2004-05-18 | 2012-08-07 | National Institute Of Transplantation Foundation | Vectors and methods for long-term immune evasion to prolong transplant viability |
EP2471922A1 (de) | 2004-05-28 | 2012-07-04 | Asuragen, Inc. | Verfahren und Zusammensetzungen mit microRNA |
US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
WO2006088490A2 (en) * | 2004-06-30 | 2006-08-24 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a non-phosphate backbone linkage |
WO2006093526A2 (en) | 2004-07-21 | 2006-09-08 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a modified or non-natural nucleobase |
AU2005330637B2 (en) | 2004-08-04 | 2012-09-20 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase |
CN101389755B (zh) | 2004-09-24 | 2014-06-11 | J.R.西姆普罗特公司 | 基因沉默 |
JP4991547B2 (ja) | 2004-09-28 | 2012-08-01 | クアーク・ファーマスーティカルス、インコーポレイテッド | 脱毛症、急性腎不全および他の疾患の治療のためのオリゴリボヌクレオチドおよびその使用の方法 |
WO2008073922A2 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | Functions and targets of let-7 micro rnas |
US8173611B2 (en) | 2004-11-12 | 2012-05-08 | Asuragen Inc. | Methods and compositions involving miRNA and miRNA inhibitor molecules |
AU2006277679B2 (en) * | 2005-03-31 | 2012-08-02 | Ronen Kahana | Creating poultry and other animals resistant to viral disease |
US20060234973A1 (en) * | 2005-04-14 | 2006-10-19 | Kevin Fitzgerald | Transcription factor RNA interference reagents and methods of use thereof |
US20070087985A1 (en) * | 2005-04-19 | 2007-04-19 | Gundersen Gregg G | MRCK-related compositions and methods |
WO2006130560A2 (en) * | 2005-05-31 | 2006-12-07 | The Trustees Of The University Of Pennsylvania | Manipulation of pten in t cells as a strategy to modulate immune responses |
US9121008B2 (en) * | 2005-08-31 | 2015-09-01 | University Of Utah Research Foundation | Development of natural killer cells and functional natural killer cell lines |
JP2007104969A (ja) * | 2005-10-13 | 2007-04-26 | Bio Think Tank Co Ltd | ショートヘアピン型RNA(shRNA)前駆体を産生するための核酸及びその利用 |
US7825099B2 (en) | 2006-01-20 | 2010-11-02 | Quark Pharmaceuticals, Inc. | Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes |
NL2000439C2 (nl) | 2006-01-20 | 2009-03-16 | Quark Biotech | Therapeutische toepassingen van inhibitoren van RTP801. |
WO2007091269A2 (en) * | 2006-02-08 | 2007-08-16 | Quark Pharmaceuticals, Inc. | NOVEL TANDEM siRNAS |
CA2663962A1 (en) * | 2006-09-19 | 2008-03-27 | Asuragen, Inc. | Mir-15, mir-26, mir-31,mir-145, mir-147, mir-188, mir-215, mir-216, mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention |
AU2007333107A1 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | miR-21 regulated genes and pathways as targets for therapeutic intervention |
CA2671270A1 (en) * | 2006-12-29 | 2008-07-17 | Asuragen, Inc. | Mir-16 regulated genes and pathways as targets for therapeutic intervention |
US20090131354A1 (en) * | 2007-05-22 | 2009-05-21 | Bader Andreas G | miR-126 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION |
PT2170403E (pt) | 2007-06-27 | 2014-07-17 | Quark Pharmaceuticals Inc | Composições e métodos para inibição da expressão de genes pró-apoptóticos |
US8361714B2 (en) | 2007-09-14 | 2013-01-29 | Asuragen, Inc. | Micrornas differentially expressed in cervical cancer and uses thereof |
CN104946591A (zh) | 2007-10-12 | 2015-09-30 | 奥卡塔治疗公司 | 制备rpe 细胞和rpe 细胞的组合物的改良方法 |
WO2009070805A2 (en) * | 2007-12-01 | 2009-06-04 | Asuragen, Inc. | Mir-124 regulated genes and pathways as targets for therapeutic intervention |
WO2009086156A2 (en) * | 2007-12-21 | 2009-07-09 | Asuragen, Inc. | Mir-10 regulated genes and pathways as targets for therapeutic intervention |
US20090263803A1 (en) * | 2008-02-08 | 2009-10-22 | Sylvie Beaudenon | Mirnas differentially expressed in lymph nodes from cancer patients |
WO2009111643A2 (en) * | 2008-03-06 | 2009-09-11 | Asuragen, Inc. | Microrna markers for recurrence of colorectal cancer |
US20090253780A1 (en) * | 2008-03-26 | 2009-10-08 | Fumitaka Takeshita | COMPOSITIONS AND METHODS RELATED TO miR-16 AND THERAPY OF PROSTATE CANCER |
WO2009126726A1 (en) * | 2008-04-08 | 2009-10-15 | Asuragen, Inc | Methods and compositions for diagnosing and modulating human papillomavirus (hpv) |
WO2009137807A2 (en) | 2008-05-08 | 2009-11-12 | Asuragen, Inc. | Compositions and methods related to mirna modulation of neovascularization or angiogenesis |
US20110190375A1 (en) * | 2008-06-11 | 2011-08-04 | Intradigm Corporation | Compositions comprising cmyc sirna and methods of use thereof |
WO2010056737A2 (en) * | 2008-11-11 | 2010-05-20 | Mirna Therapeutics, Inc. | Methods and compositions involving mirnas in cancer stem cells |
CN101525388B (zh) * | 2009-02-20 | 2012-02-01 | 中国人民解放军第四军医大学 | 特异性双链rna结合蛋白嵌合体及其在病毒感染性疾病中的应用 |
US20100257634A1 (en) * | 2009-04-03 | 2010-10-07 | Venganza Inc. | Bioassay for gene silencing constructs |
WO2013040251A2 (en) | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease |
JP2015112062A (ja) * | 2013-12-11 | 2015-06-22 | 国立大学法人豊橋技術科学大学 | Rna分子にヌクレアーゼ耐性能を付与する方法、ヌクレアーゼ耐性能を有するキメラrna分子 |
CN110229839B (zh) * | 2019-06-04 | 2021-06-08 | 中国农业大学 | 一种提升大肠杆菌dsRNA表达产率的方法 |
EP3760718A1 (de) | 2019-07-04 | 2021-01-06 | Medizinische Hochschule Hannover | Gewebe zur verwendung als allogenes oder xenogenes transplantat und verfahren zu dessen herstellung |
AU2020329166A1 (en) | 2019-08-09 | 2022-03-03 | Nutcracker Therapeutics, Inc. | Microfluidic apparatus and methods of use thereof |
WO2023118546A2 (en) | 2021-12-23 | 2023-06-29 | Boehringer Ingelheim International Gmbh | METHODS AND MOLECULES FOR RNA INTERFERENCE (RNAi) |
CN115011584A (zh) * | 2022-06-23 | 2022-09-06 | 中国水产科学研究院淡水渔业研究中心 | 青虾Cathepsin D基因及其dsRNA的应用 |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5614395A (en) | 1988-03-08 | 1997-03-25 | Ciba-Geigy Corporation | Chemically regulatable and anti-pathogenic DNA sequences and uses thereof |
US5789214A (en) | 1988-03-08 | 1998-08-04 | Novartis Finance Corporation | Method of inducing gene transcription in a plant |
US5639949A (en) | 1990-08-20 | 1997-06-17 | Ciba-Geigy Corporation | Genes for the synthesis of antipathogenic substances |
WO1992009696A1 (en) | 1990-11-23 | 1992-06-11 | Plant Genetic Systems, N.V. | Process for transforming monocotyledonous plants |
US6270989B1 (en) | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
US5641670A (en) | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
NZ245015A (en) | 1991-11-05 | 1995-12-21 | Transkaryotic Therapies Inc | Delivery of human growth hormone through the administration of transfected cell lines encoding human growth hormone, which are physically protected from host immune response; the transfected cells and their production |
US5593874A (en) | 1992-03-19 | 1997-01-14 | Monsanto Company | Enhanced expression in plants |
TW402639B (en) | 1992-12-03 | 2000-08-21 | Transkaryotic Therapies Inc | Protein production and protein delivery |
US6130092A (en) * | 1994-07-04 | 2000-10-10 | Max-Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. | Ribozyme gene library and method for making |
US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
US6025192A (en) | 1996-09-20 | 2000-02-15 | Cold Spring Harbor Laboratory | Modified retroviral vectors |
US6255071B1 (en) | 1996-09-20 | 2001-07-03 | Cold Spring Harbor Laboratory | Mammalian viral vectors and their uses |
CA2278734A1 (en) * | 1997-01-23 | 1998-07-30 | Immusol Incorporated | Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries |
US6506559B1 (en) * | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
AUPP249298A0 (en) * | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
EP1147204A1 (de) * | 1999-01-28 | 2001-10-24 | Medical College Of Georgia Research Institute, Inc. | Zusammensetzung und verfahren zur in vivo und in vitro abschwächung der genexpression mittels dobbelsträngigem rna |
US5998148A (en) * | 1999-04-08 | 1999-12-07 | Isis Pharmaceuticals Inc. | Antisense modulation of microtubule-associated protein 4 expression |
US6326193B1 (en) * | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
US20030084471A1 (en) * | 2000-03-16 | 2003-05-01 | David Beach | Methods and compositions for RNA interference |
EP1272630A2 (de) * | 2000-03-16 | 2003-01-08 | Genetica, Inc. | Methoden und zusammensetzungen zur interferenz durch rna |
AU2001249622B2 (en) * | 2000-03-30 | 2007-06-07 | Massachusetts Institute Of Technology | RNA sequence-specific mediators of RNA interference |
HU230458B1 (hu) * | 2000-12-01 | 2016-07-28 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Az RNS interferenciát közvetítő kis RNS molekulák |
US20030198627A1 (en) * | 2001-09-01 | 2003-10-23 | Gert-Jan Arts | siRNA knockout assay method and constructs |
JP3642573B2 (ja) * | 2001-11-28 | 2005-04-27 | 株式会社東京大学Tlo | siRNA発現システムおよびこれを用いた機能遺伝子ノックダウン細胞等の生産方法 |
US20050164210A1 (en) * | 2004-01-23 | 2005-07-28 | Vivek Mittal | Regulated polymerase III expression systems and related methods |
-
2002
- 2002-01-22 US US10/055,797 patent/US20030084471A1/en not_active Abandoned
-
2003
- 2003-01-22 WO PCT/US2003/001963 patent/WO2003062394A2/en active Search and Examination
- 2003-01-22 ES ES03732052T patent/ES2368039T3/es not_active Expired - Lifetime
- 2003-01-22 AT AT03732052T patent/ATE513911T1/de active
- 2003-01-22 SI SI200332042T patent/SI1546174T1/sl unknown
- 2003-01-22 CA CA002473944A patent/CA2473944A1/en not_active Abandoned
- 2003-01-22 JP JP2003562262A patent/JP4758067B2/ja not_active Expired - Lifetime
- 2003-01-22 EP EP03732052A patent/EP1546174B1/de not_active Expired - Lifetime
- 2003-01-22 DK DK03732052.0T patent/DK1546174T3/da active
-
2005
- 2005-08-08 HK HK05106782.4A patent/HK1073660A1/xx not_active IP Right Cessation
-
2011
- 2011-02-03 JP JP2011021889A patent/JP2011135882A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
SI1546174T1 (sl) | 2011-12-30 |
EP1546174A4 (de) | 2007-05-09 |
EP1546174A2 (de) | 2005-06-29 |
HK1073660A1 (en) | 2005-10-14 |
CA2473944A1 (en) | 2003-07-31 |
EP1546174B1 (de) | 2011-06-22 |
WO2003062394A2 (en) | 2003-07-31 |
ES2368039T3 (es) | 2011-11-11 |
WO2003062394A3 (en) | 2005-05-06 |
JP2005533484A (ja) | 2005-11-10 |
JP4758067B2 (ja) | 2011-08-24 |
JP2011135882A (ja) | 2011-07-14 |
US20030084471A1 (en) | 2003-05-01 |
WO2003062394A9 (en) | 2003-10-02 |
DK1546174T3 (da) | 2011-10-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE513911T1 (de) | Verfahren und zusammensetzungen für rna- interferenz | |
WO2001068836A3 (en) | Methods and compositions for rna interference | |
WO2006073601A3 (en) | Methods and compositions for rna interference | |
ATE458064T1 (de) | Verfahren zur wirksamen rna-interferenz in säugerzellen | |
GB0325653D0 (en) | CST emulsions | |
WO2006078414A3 (en) | METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE | |
WO2003064625A3 (en) | Oligonucleotide compositions with enhanced efficiency | |
WO2006119266A3 (en) | Human micrornas and methods for inhibiting same | |
EP1589118A3 (de) | Verfahren und Zusammensetzungen zur Ermittlung der Methylierung von CpG | |
MXPA05001355A (es) | Formas nuevas adicionales de moleculas de arn de interferencia. | |
WO2004013280A3 (en) | ALLELE-SPECIFIC siRNA-MEDIATED GENE SILENCING | |
WO2004011624A3 (en) | Double stranded rna structures and constructs, and methods for generating and using the same | |
GB2406169A (en) | Methods and compositions relating to labeled rna molecules that reduce gene expression | |
ATE531796T1 (de) | Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination | |
DE112005001317A5 (de) | Polymorphismen im NOD2/Card15 Gen | |
TNSN08252A1 (en) | Sirna anti myosine va et depigmentation de la peau | |
WO2007147067A3 (en) | Methods and compositions for regulating cell cycle progression | |
EP1987165A4 (de) | Gene mit einfluss auf die menschliche gedächtnisleistung | |
HK1159988A1 (en) | Compositions and methods for inhibiting expression of factor v leiden mutant gene | |
WO2005017127A3 (en) | Rna interference compositions and methods | |
DE602004032114D1 (de) | Rna-interferenz für das gen znfn3a1 als verfahren zur hemmung des wachstums von krebszellen | |
DE60323267D1 (en) | Rna-bioassay | |
WO2006128084A3 (en) | Trps1-mediated modulation of hair growth | |
ATE381615T1 (de) | Nukleinsäure-enzym zur spaltung von rna | |
ES2190866A1 (es) | Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 1546174 Country of ref document: EP |