ATE458064T1 - Verfahren zur wirksamen rna-interferenz in säugerzellen - Google Patents

Verfahren zur wirksamen rna-interferenz in säugerzellen

Info

Publication number
ATE458064T1
ATE458064T1 AT03756319T AT03756319T ATE458064T1 AT E458064 T1 ATE458064 T1 AT E458064T1 AT 03756319 T AT03756319 T AT 03756319T AT 03756319 T AT03756319 T AT 03756319T AT E458064 T1 ATE458064 T1 AT E458064T1
Authority
AT
Austria
Prior art keywords
rna interference
mammal cells
effective rna
effective
mammal
Prior art date
Application number
AT03756319T
Other languages
English (en)
Inventor
Dun Yang
J Bishop
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE458064T1 publication Critical patent/ATE458064T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26003—Ribonuclease III (3.1.26.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT03756319T 2002-05-31 2003-05-29 Verfahren zur wirksamen rna-interferenz in säugerzellen ATE458064T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38501102P 2002-05-31 2002-05-31
PCT/US2003/017178 WO2003102214A2 (en) 2002-05-31 2003-05-29 Method for efficient rna interference in mammalian cells

Publications (1)

Publication Number Publication Date
ATE458064T1 true ATE458064T1 (de) 2010-03-15

Family

ID=29712121

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03756319T ATE458064T1 (de) 2002-05-31 2003-05-29 Verfahren zur wirksamen rna-interferenz in säugerzellen

Country Status (8)

Country Link
US (1) US8119610B2 (de)
EP (1) EP1537227B1 (de)
JP (1) JP2005536195A (de)
AT (1) ATE458064T1 (de)
AU (1) AU2003253618B2 (de)
CA (1) CA2486658C (de)
DE (1) DE60331321D1 (de)
WO (1) WO2003102214A2 (de)

Families Citing this family (39)

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US20030166282A1 (en) * 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
WO2003064625A2 (en) 2002-02-01 2003-08-07 Sequitur, Inc. Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP1572923A4 (de) * 2002-03-06 2007-10-31 Rigel Pharmaceuticals Inc Neues verfahren zur zuführung und intrazellulären synthese von sirna-molekülen
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
US20100075423A1 (en) * 2002-06-12 2010-03-25 Life Technologies Corporation Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
US20040033602A1 (en) * 2002-06-12 2004-02-19 Ambion, Inc. Methods and compositions relating to polypeptides with RNase III domains that mediate RNA interference
WO2004015062A2 (en) * 2002-08-12 2004-02-19 New England Biolabs, Inc. Methods and compositions relating to gene silencing
EP2295604B1 (de) 2004-02-09 2015-04-08 Thomas Jefferson University Krebsdiagnose und Behandlung durch microRNA, die sich in der Nähe von Krebs-verbindenen Chromosom-Merkmale befinden
CA2559955C (en) * 2004-03-15 2016-02-16 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
CA2561741C (en) 2004-04-05 2016-09-27 Alnylam Pharmaceuticals, Inc. Processes and reagents for oligonucleotide synthesis and purification
AU2005325262B2 (en) 2004-04-27 2011-08-11 Alnylam Pharmaceuticals, Inc. Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
WO2006073458A2 (en) * 2004-04-30 2006-07-13 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a c5-modified pyrimidine
CA2572151A1 (en) * 2004-06-30 2006-08-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
US7579451B2 (en) 2004-07-21 2009-08-25 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
US7632932B2 (en) 2004-08-04 2009-12-15 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
CN112480257A (zh) 2005-03-23 2021-03-12 根马布股份公司 用于治疗多发性骨髓瘤的cd38抗体
EP1968622B1 (de) 2006-01-05 2014-08-27 The Ohio State University Research Foundation Mikro-rna-expressions-anomalien in pankreas- und azinus-tumoren
ES2465600T3 (es) 2006-01-05 2014-06-06 The Ohio State University Research Foundation Métodos basados en microARN y composiciones para el diagnóstico y tratamiento de cánceres sólidos del páncreas
CA2633674A1 (en) 2006-01-05 2007-07-19 The Ohio State University Research Foundation Microrna-based methods and compositions for the diagnosis, prognosis and treatment of lung cancer
US20100286232A1 (en) 2006-03-02 2010-11-11 The Ohio State University Microrna expression profile associated with pancreatic cancer
EP1996731A2 (de) 2006-03-20 2008-12-03 The Ohio State University Research Foundation Mikrorna-fingerabdrücke während humaner megakaryozytopoiese
ES2442890T3 (es) 2006-07-13 2014-02-14 The Ohio State University Research Foundation Métodos y composiciones basados en micro-ARN para el diagnóstico y el tratamiento de enfermedades relacionadas con el colon
WO2008115387A2 (en) * 2007-03-15 2008-09-25 University Hospitals Of Cleveland Screening, diagnosing, treating and prognosis of pathophysiologic states by rna regulation
WO2009012263A2 (en) * 2007-07-18 2009-01-22 The Trustees Of Columbia University In The City Of New York Tissue-specific micrornas and compositions and uses thereof
US8367632B2 (en) 2007-07-31 2013-02-05 Ohio State University Research Foundation Methods for reverting methylation by targeting methyltransferases
CN101835902B (zh) 2007-08-03 2014-03-26 俄亥俄州立大学研究基金会 编码ncrna的超保守区域
AU2008288806B2 (en) 2007-08-22 2014-11-27 The Ohio State University Research Foundation Methods and compositions for inducing deregulation of EphA7 and Erk phosphorylation in human acute leukemias
CN101939446B (zh) 2007-09-06 2015-02-11 俄亥俄州立大学研究基金会 人类卵巢癌中的微小rna特征
US20100285471A1 (en) 2007-10-11 2010-11-11 The Ohio State University Research Foundation Methods and Compositions for the Diagnosis and Treatment of Esphageal Adenocarcinomas
US20140134728A1 (en) 2011-06-01 2014-05-15 Inserm (Institut National De La Sante Et De La Recherche Medicale) Methods for adjusting expression of mitochondrial genome by microrna
HK1197273A1 (en) 2011-10-14 2015-01-09 The Ohio State University Methods and materials related to ovarian cancer
WO2013060894A1 (en) 2011-10-27 2013-05-02 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for the treatment and diagnosis of atherosclerosis
EP2836219A1 (de) 2012-04-10 2015-02-18 INSERM - Institut National de la Santé et de la Recherche Médicale Verfahren zur behandlung nichtalkoholischer steatohepatitis
WO2013153139A1 (en) 2012-04-11 2013-10-17 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for the treatment and diagnosis of acute leukemia
EP3079727A1 (de) 2013-12-12 2016-10-19 INSERM - Institut National de la Santé et de la Recherche Médicale Verfahren zur vorbeugung und behandlung von diabetischer kardiomyopathie mithilfe von mir-424/322
US20220211741A1 (en) 2019-04-18 2022-07-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for the treatment and prognosis of cancer
WO2024189064A1 (en) 2023-03-14 2024-09-19 Institut National de la Santé et de la Recherche Médicale The circular rna circltbp2 as a biomarker and biotarget in intrahepatic cholangiocarcinomas
WO2025061810A1 (en) 2023-09-20 2025-03-27 Institut National de la Santé et de la Recherche Médicale Use of a mirna inhibitor for the treatment of osteogenesis imperfecta

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6737512B2 (en) * 1996-06-06 2004-05-18 Isis Pharmaceuticals, Inc. Human RNase III and compositions and uses thereof
WO2001068836A2 (en) * 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
US20030114410A1 (en) * 2000-08-08 2003-06-19 Technion Research And Development Foundation Ltd. Pharmaceutical compositions and methods useful for modulating angiogenesis and inhibiting metastasis and tumor fibrosis
US6329203B1 (en) * 2000-09-08 2001-12-11 Isis Pharmaceuticals, Inc. Antisense modulation of glioma-associated oncogene-1 expression
US7067322B2 (en) * 2001-07-10 2006-06-27 Wisconsin Alumni Research Foundation Fusion protein arrays on metal substrates for surface plasmon resonance imaging
US20040033602A1 (en) 2002-06-12 2004-02-19 Ambion, Inc. Methods and compositions relating to polypeptides with RNase III domains that mediate RNA interference
WO2004015062A2 (en) 2002-08-12 2004-02-19 New England Biolabs, Inc. Methods and compositions relating to gene silencing
EP1706511A4 (de) 2004-01-23 2010-04-07 New England Biolabs Inc Zusammensetzungen und verfahren zur erzeugung kurzer doppelsträngiger rna unter verwendung von mutierter rnase iii

Also Published As

Publication number Publication date
EP1537227B1 (de) 2010-02-17
US8119610B2 (en) 2012-02-21
CA2486658A1 (en) 2003-12-11
EP1537227A4 (de) 2007-09-26
AU2003253618B2 (en) 2007-11-15
WO2003102214A2 (en) 2003-12-11
WO2003102214A3 (en) 2005-02-17
US20040014113A1 (en) 2004-01-22
DE60331321D1 (de) 2010-04-01
EP1537227A2 (de) 2005-06-08
JP2005536195A (ja) 2005-12-02
AU2003253618A1 (en) 2003-12-19
CA2486658C (en) 2014-07-29

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