DE60331321D1 - Verfahren zur wirksamen rna-interferenz in säugerzellen - Google Patents
Verfahren zur wirksamen rna-interferenz in säugerzellenInfo
- Publication number
- DE60331321D1 DE60331321D1 DE60331321T DE60331321T DE60331321D1 DE 60331321 D1 DE60331321 D1 DE 60331321D1 DE 60331321 T DE60331321 T DE 60331321T DE 60331321 T DE60331321 T DE 60331321T DE 60331321 D1 DE60331321 D1 DE 60331321D1
- Authority
- DE
- Germany
- Prior art keywords
- mammalian cells
- rna interference
- effective rna
- effective
- inhibition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
- C12Y301/26003—Ribonuclease III (3.1.26.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38501102P | 2002-05-31 | 2002-05-31 | |
PCT/US2003/017178 WO2003102214A2 (en) | 2002-05-31 | 2003-05-29 | Method for efficient rna interference in mammalian cells |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60331321D1 true DE60331321D1 (de) | 2010-04-01 |
Family
ID=29712121
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60331321T Expired - Lifetime DE60331321D1 (de) | 2002-05-31 | 2003-05-29 | Verfahren zur wirksamen rna-interferenz in säugerzellen |
Country Status (8)
Country | Link |
---|---|
US (1) | US8119610B2 (de) |
EP (1) | EP1537227B1 (de) |
JP (1) | JP2005536195A (de) |
AT (1) | ATE458064T1 (de) |
AU (1) | AU2003253618B2 (de) |
CA (1) | CA2486658C (de) |
DE (1) | DE60331321D1 (de) |
WO (1) | WO2003102214A2 (de) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030166282A1 (en) | 2002-02-01 | 2003-09-04 | David Brown | High potency siRNAS for reducing the expression of target genes |
US20060009409A1 (en) | 2002-02-01 | 2006-01-12 | Woolf Tod M | Double-stranded oligonucleotides |
CA2474910A1 (en) | 2002-02-01 | 2003-08-07 | Sequitur, Inc. | Oligonucleotide compositions with enhanced efficiency |
EP1572923A4 (de) * | 2002-03-06 | 2007-10-31 | Rigel Pharmaceuticals Inc | Neues verfahren zur zuführung und intrazellulären synthese von sirna-molekülen |
US20100075423A1 (en) * | 2002-06-12 | 2010-03-25 | Life Technologies Corporation | Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference |
US20040248094A1 (en) * | 2002-06-12 | 2004-12-09 | Ford Lance P. | Methods and compositions relating to labeled RNA molecules that reduce gene expression |
EP1532271A4 (de) * | 2002-06-12 | 2006-10-18 | Ambion Inc | Verfahren und zusammensetzungen im zusammenhang mit polypeptiden mit rnase-iii-domänen, die rna-interferenz vermitteln |
US7700758B2 (en) * | 2002-08-12 | 2010-04-20 | New England Biolabs, Inc. | Methods and compositions relating to gene silencing |
EP2295604B1 (de) | 2004-02-09 | 2015-04-08 | Thomas Jefferson University | Krebsdiagnose und Behandlung durch microRNA, die sich in der Nähe von Krebs-verbindenen Chromosom-Merkmale befinden |
AU2005222965B8 (en) * | 2004-03-15 | 2010-07-01 | City Of Hope | Methods and compositions for the specific inhibition of gene expression by double-stranded RNA |
EP1737878A2 (de) | 2004-04-05 | 2007-01-03 | Alnylam Pharmaceuticals Inc. | Verfahren und reagentien für die synthese und aufreinigung von oligonukleotiden |
WO2006078278A2 (en) | 2004-04-27 | 2006-07-27 | Alnylam Pharmaceuticals, Inc. | Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety |
US7674778B2 (en) * | 2004-04-30 | 2010-03-09 | Alnylam Pharmaceuticals | Oligonucleotides comprising a conjugate group linked through a C5-modified pyrimidine |
JP2008504840A (ja) * | 2004-06-30 | 2008-02-21 | アルニラム ファーマスーティカルズ インコーポレイテッド | 非リン酸骨格結合を含むオリゴヌクレオチド |
CA2574088C (en) | 2004-07-21 | 2013-09-17 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a modified or non-natural nucleobase |
AU2005330637B2 (en) | 2004-08-04 | 2012-09-20 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase |
EA037929B1 (ru) | 2005-03-23 | 2021-06-08 | Генмаб А/С | Антитела к cd38 человека и их применение |
US8148069B2 (en) | 2006-01-05 | 2012-04-03 | The Ohio State University | MicroRNA-based methods and compositions for the diagnosis, prognosis and treatment of solid cancers |
ES2553442T3 (es) | 2006-01-05 | 2015-12-09 | The Ohio State University Research Foundation | Procedimientos basados en los microARN para el diagnóstico, pronóstico y tratamiento del cáncer de pulmón |
CN101384273B (zh) | 2006-01-05 | 2013-07-10 | 俄亥俄州立大学研究基金会 | 胰腺内分泌和腺泡肿瘤中的微小rna表达异常 |
EP1991706B1 (de) | 2006-03-02 | 2014-01-15 | The Ohio State University Research Foundation | Mikro-rna-expressionsprofil im zusammenhang mit bauchspeicheldrüsenkrebs |
ES2446362T3 (es) | 2006-03-20 | 2014-03-07 | The Ohio State University Research Foundation | Huellas de microARN durante megacariocipoyesis humana |
ES2434075T3 (es) | 2006-07-13 | 2013-12-13 | The Ohio State University Research Foundation | MIR-203 para el diagnóstico de adenocarcinoma de colon con mal pronóstico de supervivencia |
US8841436B2 (en) * | 2007-03-15 | 2014-09-23 | University Hospitals Cleveland Medical Center | Screening, diagnosing, treating and prognosis of pathophysiologic status by RNA regulation |
WO2009012263A2 (en) * | 2007-07-18 | 2009-01-22 | The Trustees Of Columbia University In The City Of New York | Tissue-specific micrornas and compositions and uses thereof |
ES2496172T3 (es) | 2007-07-31 | 2014-09-18 | The Ohio State University Research Foundation | Métodos para invertir la metilación por selección dirigida de DNMT3A y DNMT3B |
US8465918B2 (en) | 2007-08-03 | 2013-06-18 | The Ohio State University Research Foundation | Ultraconserved regions encoding ncRNAs |
US8466119B2 (en) | 2007-08-22 | 2013-06-18 | The Ohio State University Research Foundation | Methods and compositions for inducing deregulation of EPHA7 and ERK phosphorylation in human acute leukemias |
WO2009033140A1 (en) | 2007-09-06 | 2009-03-12 | The Ohio State University Research Foundation | Microrna signatures in human ovarian cancer |
EP2212440A4 (de) | 2007-10-11 | 2011-04-06 | Univ Ohio State Res Found | Verfahren und zusammensetzungen zur diagnose und behandlung von adenokarzinomen der speiseröhre |
WO2012164058A1 (en) | 2011-06-01 | 2012-12-06 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for adjusting expression of mitochondrial genome by microrna |
EP2766500A4 (de) | 2011-10-14 | 2015-10-14 | Univ Ohio State | Verfahren und materialien in verbindung mit eierstockkrebs |
WO2013060894A1 (en) | 2011-10-27 | 2013-05-02 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for the treatment and diagnosis of atherosclerosis |
JP2015518475A (ja) | 2012-04-10 | 2015-07-02 | インサーム(インスティテュ ナシオナル ドゥ ラ サンテ エ ドゥ ラルシェルシェ メディカル)Inserm(Institut National Dela Sante Etde La Recherche Medicale) | 非アルコール性脂肪性肝炎の治療方法 |
WO2013153139A1 (en) | 2012-04-11 | 2013-10-17 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for the treatment and diagnosis of acute leukemia |
WO2015086828A1 (en) | 2013-12-12 | 2015-06-18 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for the prevention and treatment of diabetic cardiomyopathy using mir-424/322 |
EP3956474A1 (de) | 2019-04-18 | 2022-02-23 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Verfahren zur behandlung und prognose von krebs |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6737512B2 (en) * | 1996-06-06 | 2004-05-18 | Isis Pharmaceuticals, Inc. | Human RNase III and compositions and uses thereof |
EP1272630A2 (de) * | 2000-03-16 | 2003-01-08 | Genetica, Inc. | Methoden und zusammensetzungen zur interferenz durch rna |
US20030114410A1 (en) * | 2000-08-08 | 2003-06-19 | Technion Research And Development Foundation Ltd. | Pharmaceutical compositions and methods useful for modulating angiogenesis and inhibiting metastasis and tumor fibrosis |
US6329203B1 (en) * | 2000-09-08 | 2001-12-11 | Isis Pharmaceuticals, Inc. | Antisense modulation of glioma-associated oncogene-1 expression |
US7067322B2 (en) * | 2001-07-10 | 2006-06-27 | Wisconsin Alumni Research Foundation | Fusion protein arrays on metal substrates for surface plasmon resonance imaging |
EP1532271A4 (de) * | 2002-06-12 | 2006-10-18 | Ambion Inc | Verfahren und zusammensetzungen im zusammenhang mit polypeptiden mit rnase-iii-domänen, die rna-interferenz vermitteln |
US7700758B2 (en) * | 2002-08-12 | 2010-04-20 | New England Biolabs, Inc. | Methods and compositions relating to gene silencing |
EP1706511A4 (de) * | 2004-01-23 | 2010-04-07 | New England Biolabs Inc | Zusammensetzungen und verfahren zur erzeugung kurzer doppelsträngiger rna unter verwendung von mutierter rnase iii |
-
2003
- 2003-05-29 JP JP2004510450A patent/JP2005536195A/ja active Pending
- 2003-05-29 US US10/448,612 patent/US8119610B2/en active Active
- 2003-05-29 CA CA2486658A patent/CA2486658C/en not_active Expired - Lifetime
- 2003-05-29 EP EP03756319A patent/EP1537227B1/de not_active Expired - Lifetime
- 2003-05-29 AT AT03756319T patent/ATE458064T1/de not_active IP Right Cessation
- 2003-05-29 WO PCT/US2003/017178 patent/WO2003102214A2/en active Search and Examination
- 2003-05-29 AU AU2003253618A patent/AU2003253618B2/en not_active Expired
- 2003-05-29 DE DE60331321T patent/DE60331321D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2486658A1 (en) | 2003-12-11 |
US8119610B2 (en) | 2012-02-21 |
CA2486658C (en) | 2014-07-29 |
EP1537227B1 (de) | 2010-02-17 |
EP1537227A4 (de) | 2007-09-26 |
WO2003102214A2 (en) | 2003-12-11 |
WO2003102214A3 (en) | 2005-02-17 |
ATE458064T1 (de) | 2010-03-15 |
AU2003253618A1 (en) | 2003-12-19 |
AU2003253618B2 (en) | 2007-11-15 |
EP1537227A2 (de) | 2005-06-08 |
US20040014113A1 (en) | 2004-01-22 |
JP2005536195A (ja) | 2005-12-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60331321D1 (de) | Verfahren zur wirksamen rna-interferenz in säugerzellen | |
ATE513911T1 (de) | Verfahren und zusammensetzungen für rna- interferenz | |
ATE513910T1 (de) | Methoden zur gensuppression mithilfe von rnai verstärkenden faktoren | |
ATE330032T1 (de) | Verfahren zur erhaltung der dns-integrität | |
HK1086036A1 (en) | Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (sina) | |
ATE507305T1 (de) | Verfahren zur nukleinsäureimmobilisierung | |
ATE533838T1 (de) | Verfahren zur verbesserten einführung von dna in bakterienzellen | |
DE602006017192D1 (de) | Verfahren zur herstellung von oligonukleotiden | |
MX2007006610A (es) | Metodo para simplificar acidos nucleicos microbianos por modificacion quimica de citosinas. | |
AU2003297474A8 (en) | Methods of inhibiting gene expression by rna interference | |
EP1546173A4 (de) | Verfahren zur herunterregulierung der expression von targetgenen in vivo durch einschleusen von interferierender rna | |
WO2007048628A3 (en) | Structures of active guide rna molecules and method of selection | |
MX2009002462A (es) | Siarn y metodos de manufactura del mismo. | |
AU2003213054A8 (en) | Rna interference mediated inhibition of polycomb group protein ezh2 gene expression using short interfering nucleic acid (sina) | |
WO2005042705A3 (en) | Short interfering rna and micro-rna compounds and methods of designing, making and using the same | |
WO2004061081A3 (en) | Sirna compounds and methods for the downregulation of gene expression | |
SG158739A1 (en) | Aggrecanase molecules | |
ATE504648T1 (de) | Rna-interferenz für das gen znfn3a1 als verfahren zur hemmung des wachstums von krebszellen | |
AU2003213057A8 (en) | Rna interference mediated inhibition of checkpoint kinase-1 (chk-1) gene expression using short interfering nucleic acid | |
WO2005045032A3 (en) | RNA INTERFERENCE MEDIATED INHIBITION OF EARLY GROWTH RESPONSE GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) | |
WO2005014811A3 (en) | RNA INTERFERENCE MEDIATED INHIBITION OF XIAP GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) | |
GB0212302D0 (en) | Method of selecting targets for gene silencing by RNA interference | |
EP1828415A4 (de) | Verfahren zur hemmung der expression von ziel-mrna unter verwendung von sirna mit einer zur ziel-mrna komplementären nukleotidsequenz | |
ATE491035T1 (de) | Verfahren zur abtrennung selektionierbarer markergensequenzen | |
DE602004030408D1 (de) | Verfahren zur Verringerung des Abblätterns von Ablagerungen in Brennkammern |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |