MX9705279A - Produccion de carboxipeptidasa b recombinante enzimaticamente activa. - Google Patents

Produccion de carboxipeptidasa b recombinante enzimaticamente activa.

Info

Publication number
MX9705279A
MX9705279A MX9705279A MX9705279A MX9705279A MX 9705279 A MX9705279 A MX 9705279A MX 9705279 A MX9705279 A MX 9705279A MX 9705279 A MX9705279 A MX 9705279A MX 9705279 A MX9705279 A MX 9705279A
Authority
MX
Mexico
Prior art keywords
procpb
enzymatically active
production
active recombinant
active cpb
Prior art date
Application number
MX9705279A
Other languages
English (en)
Inventor
Jacob R Hartman
Netta Fulga
Simona Mendelovitch
Marian Gorecki
Original Assignee
Bio Technology General Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Technology General Corp filed Critical Bio Technology General Corp
Publication of MX9705279A publication Critical patent/MX9705279A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invencion proporciona un método para producir CPB enzimáticamente activo el cual comprende el tratar una célula recombinante DNA codificando ProCPB, de manera que el DNA dirige la expresion del ProCPB, recuperar de la célula el ProCPB así expresado, tratar el ProCPB recuperado bajo condiciones que permitan el doblado del ProCPB, someter el ProCPB doblado a segmentado enzimático para producir CPB enzimáticamente activo y purificar el CPB enzimáticamente activo.
MX9705279A 1995-01-25 1997-07-11 Produccion de carboxipeptidasa b recombinante enzimaticamente activa. MX9705279A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37823395A 1995-01-25 1995-01-25

Publications (1)

Publication Number Publication Date
MX9705279A true MX9705279A (es) 1998-06-30

Family

ID=23492292

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9705279A MX9705279A (es) 1995-01-25 1997-07-11 Produccion de carboxipeptidasa b recombinante enzimaticamente activa.

Country Status (22)

Country Link
US (1) US5948668A (es)
EP (1) EP0871718B1 (es)
JP (1) JP4250716B2 (es)
KR (1) KR100566136B1 (es)
CN (1) CN1144874C (es)
AT (1) ATE358717T1 (es)
AU (1) AU698889B2 (es)
BR (1) BR9606795A (es)
CA (1) CA2210242C (es)
CZ (1) CZ292541B6 (es)
DE (1) DE69637011T2 (es)
DK (1) DK0871718T3 (es)
ES (1) ES2284167T3 (es)
HK (1) HK1014986A1 (es)
HU (1) HU225673B1 (es)
IL (1) IL116696A (es)
MX (1) MX9705279A (es)
NZ (1) NZ302874A (es)
PL (1) PL183228B1 (es)
PT (1) PT871718E (es)
WO (1) WO1996023064A1 (es)
ZA (1) ZA96515B (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU707689B2 (en) 1995-08-16 1999-07-15 Astrazeneca Ab Mutated Carboxypeptidase B enzyme conjugated to a tumour targeted antibody for use in Antibody Directed Enzyme Prodrug Therapy
ATE498409T1 (de) 1998-08-06 2011-03-15 Mountain View Pharmaceuticals Peg-uricase konjugate und verwendung davon
US20040117863A1 (en) * 1998-09-18 2004-06-17 Edge Michael D. Transgenically produced fusion proteins
DE19915938A1 (de) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
HUP0500423A2 (hu) * 1999-09-17 2005-08-29 Gtc Biotherapeutics, Inc. Transzgenikus úton előállított fúziós fehérjék
DE60023928T2 (de) 1999-09-29 2006-07-27 Lexicon Genetics Inc., The Woodlands Humane carboxypeptidasen und diese kodierende polynukleotide
EP1632575B1 (en) * 1999-09-29 2009-01-28 Lexicon Pharmaceuticals, Inc. Human carboxypeptidases and polynucleotides encoding the same
WO2001051624A2 (en) * 2000-01-12 2001-07-19 Eli Lilly And Company Carboxypeptidase b free of animal products and contaminating enyzme activity
EP1538203B1 (en) * 2003-12-05 2010-01-20 Roche Diagnostics GmbH Recombinantly expressed carboxypeptidase B and purification thereof
ES2337684T3 (es) * 2003-12-05 2010-04-28 F. Hoffmann-La Roche Ag Carboxipeptidasa b recombinante y su purificacion.
JP5114201B2 (ja) * 2004-09-27 2013-01-09 サノフイ−アベンテイス・ドイチユラント・ゲー・エム・ベー・ハー 組み換えカルボキシペプチダーゼｂ
SG10201706384VA (en) 2005-04-11 2017-09-28 Crealta Pharmaceuticals Llc A variant form of urate oxidase and use thereof
US20080159976A1 (en) * 2005-04-11 2008-07-03 Jacob Hartman Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase
WO2006110819A2 (en) 2005-04-11 2006-10-19 Savient Pharmaceuticals, Inc. Variant forms of urate oxidase and use thereof
US8148123B2 (en) 2005-04-11 2012-04-03 Savient Pharmaceuticals, Inc. Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase
US20090081122A1 (en) * 2005-05-23 2009-03-26 Universite De Geneve Injectable superparamagnetic nanoparticles for treatment by hyperthermia and use for forming an hyperthermic implant
ES2532804T3 (es) 2006-04-12 2015-03-31 Crealta Pharmaceuticals Llc Purificación de proteínas con tensioactivo catiónico
CN101058805B (zh) * 2007-03-21 2011-09-07 北京贯虹科技有限公司 突变羧肽酶原b及突变羧肽酶b的生产方法
US8993714B2 (en) * 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
WO2010132363A1 (en) 2009-05-11 2010-11-18 Imiplex Llc Method of protein nanostructure fabrication
BRPI1010069A2 (pt) 2009-06-25 2016-03-15 Savient Pharmaceuticals Inc "método para prevenir reações à infusão durante terapia por uricase peguilada em pacientes; e método para diagnosticar se um paciente tratado com uricase peguilada desenvolverá reações à infusão ou desenvolverá liberação de uricase peguilada mediada por anticorpo sem a medição de títulos de anticorpos anti-peg e anti-uricase peguilada"
CN101967467B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组羧肽酶b的生产及应用
US11918624B2 (en) * 2020-06-10 2024-03-05 Kelsius Laboratories LLC Therapeutic composition for use in the treatment of COVID-19 and other cytokine storm associated disorders

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK124483B (da) * 1967-12-04 1972-10-23 Bayer Ag Fremgangsmåde til rensning af carboxypeptidase B.
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
HU190129B (en) * 1983-07-11 1986-08-28 Reanal Finomvegyszergyar,Hu Process for the isolation of carboxypeptidase b enzyme from mammal pancreas
US5206161A (en) * 1991-02-01 1993-04-27 Genentech, Inc. Human plasma carboxypeptidase B
FR2692907B1 (fr) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
JP3472587B2 (ja) * 1992-08-28 2003-12-02 アベンティス ファーマ株式会社 骨関連カルボキシペプチダーゼ様タンパク質およびその製造法
CA2175834A1 (en) * 1993-11-16 1995-05-26 Jeffrey Tobin Fayerman Dna sequences encoding porcine pancreatic carboxypeptidase b

Also Published As

Publication number Publication date
DE69637011D1 (de) 2007-05-16
ZA96515B (en) 1996-08-15
NZ302874A (en) 1998-11-25
CN1177377A (zh) 1998-03-25
AU4903496A (en) 1996-08-14
PL321499A1 (en) 1997-12-08
CN1144874C (zh) 2004-04-07
PT871718E (pt) 2007-06-26
HU225673B1 (en) 2007-06-28
KR19980701652A (ko) 1998-06-25
WO1996023064A1 (en) 1996-08-01
JP4250716B2 (ja) 2009-04-08
CZ225897A3 (cs) 1998-10-14
US5948668A (en) 1999-09-07
IL116696A (en) 1999-08-17
EP0871718A1 (en) 1998-10-21
EP0871718B1 (en) 2007-04-04
DK0871718T3 (da) 2007-07-02
HK1014986A1 (en) 1999-10-08
CA2210242A1 (en) 1996-08-01
DE69637011T2 (de) 2007-12-13
PL183228B1 (pl) 2002-06-28
KR100566136B1 (ko) 2006-11-10
IL116696A0 (en) 1996-05-14
EP0871718A4 (en) 2000-06-07
CZ292541B6 (cs) 2003-10-15
CA2210242C (en) 2010-04-27
ES2284167T3 (es) 2007-11-01
BR9606795A (pt) 1997-12-30
AU698889B2 (en) 1998-11-12
ATE358717T1 (de) 2007-04-15
HUP9800091A2 (hu) 1998-05-28
HUP9800091A3 (en) 2004-03-29
JPH11503002A (ja) 1999-03-23

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