MX369109B - Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. - Google Patents
Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas.Info
- Publication number
- MX369109B MX369109B MX2016001396A MX2016001396A MX369109B MX 369109 B MX369109 B MX 369109B MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 369109 B MX369109 B MX 369109B
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- host cells
- eukaryotic host
- selection vectors
- folate receptor
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000013598 vector Substances 0.000 title 1
- 239000013604 expression vector Substances 0.000 abstract 4
- 102000006815 folate receptor Human genes 0.000 abstract 3
- 108020005243 folate receptor Proteins 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 229940014144 folate Drugs 0.000 abstract 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract 1
- 235000019152 folic acid Nutrition 0.000 abstract 1
- 239000011724 folic acid Substances 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 238000010187 selection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361860439P | 2013-07-31 | 2013-07-31 | |
| PCT/IB2014/063517 WO2015015419A1 (en) | 2013-07-31 | 2014-07-29 | Novel selection vectors and methods of selecting eukaryotic host cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016001396A MX2016001396A (es) | 2016-05-05 |
| MX369109B true MX369109B (es) | 2019-10-29 |
Family
ID=51383909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016001396A MX369109B (es) | 2013-07-31 | 2014-07-29 | Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US11807855B2 (enExample) |
| EP (3) | EP3412684B1 (enExample) |
| JP (1) | JP6576924B2 (enExample) |
| KR (1) | KR102288233B1 (enExample) |
| CN (2) | CN105593239B (enExample) |
| AU (1) | AU2014297966B2 (enExample) |
| BR (1) | BR112016001182A2 (enExample) |
| CA (1) | CA2918117C (enExample) |
| DK (2) | DK3412684T3 (enExample) |
| ES (2) | ES2920510T3 (enExample) |
| HU (1) | HUE058885T2 (enExample) |
| IL (1) | IL243352B (enExample) |
| LT (2) | LT3027646T (enExample) |
| MX (1) | MX369109B (enExample) |
| PL (2) | PL3027646T3 (enExample) |
| PT (2) | PT3412684T (enExample) |
| RU (1) | RU2716977C2 (enExample) |
| SG (1) | SG11201510625TA (enExample) |
| WO (1) | WO2015015419A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9534226B2 (en) | 2009-02-27 | 2017-01-03 | Novartis Ag | Expression vector system comprising two selection markers |
| PL3027646T3 (pl) | 2013-07-31 | 2019-03-29 | Novartis Ag | Nowe wektory selekcyjne i sposoby selekcji eukariotycznych komórek gospodarza |
| EP4530350A3 (en) * | 2015-10-09 | 2025-06-25 | Genzyme Corporation | Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting |
| EP3521435A1 (en) | 2018-02-06 | 2019-08-07 | Bayer AG | A high stringency selection system for protein expression in proline-auxotrophic cells |
| JP2021536263A (ja) * | 2018-09-07 | 2021-12-27 | メディミューン，エルエルシー | 細胞選択の方法 |
| PE20221416A1 (es) | 2019-09-18 | 2022-09-20 | Novartis Ag | Proteinas de fusion nkg2d y sus usos |
| WO2022107090A1 (en) | 2020-11-23 | 2022-05-27 | Novartis Ag | Expression technology for antibody constructs |
| WO2022229854A1 (en) | 2021-04-29 | 2022-11-03 | Novartis Ag | Hypersialylating cells |
| US20250051758A1 (en) * | 2021-12-21 | 2025-02-13 | Absci Corporation | FolA Selection Assay to Identify Strains with Increased Soluble Target Protein Expression |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4771036A (en) | 1986-02-10 | 1988-09-13 | Trustees Of Columbia University In The City Of New York | Method and ophthalmic composition for the prevention and reversal of cataracts |
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| CA2096222C (en) | 1990-11-13 | 1998-12-29 | Stephen D. Lupton | Bifunctional selectable fusion genes |
| EP0724639B1 (en) | 1991-03-29 | 2001-01-31 | Genentech, Inc. | Methods for selection of recombinant host cells expressing high levels of a desired protein |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| US6106825A (en) | 1997-05-07 | 2000-08-22 | University Of Florida | Entomopoxvirus-vertebrate gene delivery vector and method |
| FR2784030B1 (fr) | 1998-10-02 | 2002-12-20 | Inst Nat Sante Rech Med | Utilisation de bloqueurs des canaux calciques et/ou cgmp-dependants pour le traitement de pathologies de la retine |
| FR2823221B1 (fr) | 2001-04-06 | 2004-04-02 | Univ Pasteur | Sequences associees a la degenerescence retinienne et applications |
| DE10256083A1 (de) | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| ES2309508T3 (es) * | 2003-03-11 | 2008-12-16 | Laboratoires Serono Sa | Vectores de expresion que comprenden el promotor de mcmv-ie2. |
| DK1716233T3 (da) | 2004-01-30 | 2009-10-26 | Maxygen Holdings Ltd | Reguleret stopkodongennemlæsning |
| US20060275794A1 (en) | 2005-03-07 | 2006-12-07 | Invitrogen Corporation | Collections of matched biological reagents and methods for identifying matched reagents |
| EP1987150B1 (en) | 2006-02-21 | 2011-06-01 | ChromaGenics B.V. | Selection of host cells expressing protein at high levels |
| BRPI0712073B8 (pt) | 2006-05-17 | 2021-05-25 | Hoffmann La Roche | ácido nucléico para produção de um polipeptídio ou proteína heteróloga |
| CN103805633B (zh) * | 2007-12-21 | 2016-09-07 | 诺华股份有限公司 | 有机化合物 |
| EP3075858B1 (en) | 2007-12-21 | 2020-01-29 | Novartis Ag | Mammalian expression vector |
| ES2413379T3 (es) | 2008-08-28 | 2013-07-16 | Novartis Ag | Presentación en la superficie celular de isoformas polipeptídicas mediante ultralectura de codón de terminación |
| WO2010029130A1 (en) | 2008-09-10 | 2010-03-18 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Neuronal viability factor and use thereof |
| CN102333875B (zh) | 2009-02-27 | 2014-04-16 | 诺华股份有限公司 | 选择表达异源蛋白质的真核细胞的方法 |
| US9534226B2 (en) | 2009-02-27 | 2017-01-03 | Novartis Ag | Expression vector system comprising two selection markers |
| CN102030754B (zh) * | 2009-09-28 | 2013-07-10 | 中国科学院微生物研究所 | 非病毒载体及其制备方法与应用 |
| PL3027646T3 (pl) | 2013-07-31 | 2019-03-29 | Novartis Ag | Nowe wektory selekcyjne i sposoby selekcji eukariotycznych komórek gospodarza |
-
2014
- 2014-07-29 PL PL14753158T patent/PL3027646T3/pl unknown
- 2014-07-29 CN CN201480054263.9A patent/CN105593239B/zh active Active
- 2014-07-29 ES ES18179678T patent/ES2920510T3/es active Active
- 2014-07-29 MX MX2016001396A patent/MX369109B/es active IP Right Grant
- 2014-07-29 AU AU2014297966A patent/AU2014297966B2/en active Active
- 2014-07-29 DK DK18179678.0T patent/DK3412684T3/da active
- 2014-07-29 PT PT181796780T patent/PT3412684T/pt unknown
- 2014-07-29 US US14/907,866 patent/US11807855B2/en active Active
- 2014-07-29 PT PT14753158T patent/PT3027646T/pt unknown
- 2014-07-29 RU RU2016106270A patent/RU2716977C2/ru active
- 2014-07-29 LT LTEP14753158.6T patent/LT3027646T/lt unknown
- 2014-07-29 CN CN202010947233.8A patent/CN112029791A/zh active Pending
- 2014-07-29 HU HUE18179678A patent/HUE058885T2/hu unknown
- 2014-07-29 LT LTEP18179678.0T patent/LT3412684T/lt unknown
- 2014-07-29 KR KR1020167002307A patent/KR102288233B1/ko active Active
- 2014-07-29 BR BR112016001182A patent/BR112016001182A2/pt not_active Application Discontinuation
- 2014-07-29 EP EP18179678.0A patent/EP3412684B1/en active Active
- 2014-07-29 WO PCT/IB2014/063517 patent/WO2015015419A1/en not_active Ceased
- 2014-07-29 EP EP14753158.6A patent/EP3027646B1/en active Active
- 2014-07-29 SG SG11201510625TA patent/SG11201510625TA/en unknown
- 2014-07-29 ES ES14753158.6T patent/ES2689136T3/es active Active
- 2014-07-29 EP EP22166577.1A patent/EP4095153A1/en not_active Withdrawn
- 2014-07-29 JP JP2016530647A patent/JP6576924B2/ja active Active
- 2014-07-29 DK DK14753158.6T patent/DK3027646T3/en active
- 2014-07-29 CA CA2918117A patent/CA2918117C/en active Active
- 2014-07-29 PL PL18179678.0T patent/PL3412684T3/pl unknown
-
2015
- 2015-12-24 IL IL243352A patent/IL243352B/en active IP Right Grant
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |