MX351174B - Microorganismos que producen acido eicosapentaenoico, composiciones de acidos grasos,y metodos para prepararlos y usos de los mismos. - Google Patents
Microorganismos que producen acido eicosapentaenoico, composiciones de acidos grasos,y metodos para prepararlos y usos de los mismos.Info
- Publication number
- MX351174B MX351174B MX2014000759A MX2014000759A MX351174B MX 351174 B MX351174 B MX 351174B MX 2014000759 A MX2014000759 A MX 2014000759A MX 2014000759 A MX2014000759 A MX 2014000759A MX 351174 B MX351174 B MX 351174B
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- microorganisms
- prepare
- fatty acid
- compositions
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 title 1
- 235000014113 dietary fatty acids Nutrition 0.000 title 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 title 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 title 1
- 235000020673 eicosapentaenoic acid Nutrition 0.000 title 1
- 229930195729 fatty acid Natural products 0.000 title 1
- 239000000194 fatty acid Substances 0.000 title 1
- 150000004665 fatty acids Chemical class 0.000 title 1
- 230000000813 microbial effect Effects 0.000 abstract 3
- 239000003921 oil Substances 0.000 abstract 3
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Birds (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Marine Sciences & Fisheries (AREA)
- Insects & Arthropods (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161510464P | 2011-07-21 | 2011-07-21 | |
| PCT/US2012/047728 WO2013013208A2 (en) | 2011-07-21 | 2012-07-20 | Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014000759A MX2014000759A (es) | 2014-05-07 |
| MX351174B true MX351174B (es) | 2017-10-04 |
Family
ID=47558749
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017012687A MX385408B (es) | 2011-07-21 | 2012-07-20 | Microorganismos que producen ácido eicosapentaenoico, composiciones de ácidos grasos, y métodos para prepararlos y usos de los mismos. |
| MX2014000759A MX351174B (es) | 2011-07-21 | 2012-07-20 | Microorganismos que producen acido eicosapentaenoico, composiciones de acidos grasos,y metodos para prepararlos y usos de los mismos. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017012687A MX385408B (es) | 2011-07-21 | 2012-07-20 | Microorganismos que producen ácido eicosapentaenoico, composiciones de ácidos grasos, y métodos para prepararlos y usos de los mismos. |
Country Status (20)
| Country | Link |
|---|---|
| US (5) | US9045785B2 (enExample) |
| EP (4) | EP4585049A2 (enExample) |
| JP (3) | JP6338285B2 (enExample) |
| KR (2) | KR102038715B1 (enExample) |
| CN (3) | CN104011217B (enExample) |
| AR (1) | AR087944A1 (enExample) |
| AU (2) | AU2012285803B2 (enExample) |
| BR (2) | BR122015020130A2 (enExample) |
| CA (1) | CA2842374C (enExample) |
| CL (2) | CL2014000156A1 (enExample) |
| DK (1) | DK3050972T3 (enExample) |
| EA (1) | EA034980B1 (enExample) |
| ES (2) | ES2774778T3 (enExample) |
| IL (1) | IL230500B (enExample) |
| MX (2) | MX385408B (enExample) |
| MY (1) | MY169454A (enExample) |
| PH (1) | PH12014500169B1 (enExample) |
| SG (1) | SG10201605966TA (enExample) |
| WO (1) | WO2013013208A2 (enExample) |
| ZA (2) | ZA201400620B (enExample) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3196326B2 (ja) | 1992-06-22 | 2001-08-06 | 石川島播磨重工業株式会社 | 偏平ブロー成形装置 |
| JP3118954B2 (ja) | 1992-05-14 | 2000-12-18 | 石川島播磨重工業株式会社 | 偏平ブロー成形装置 |
| JP3125443B2 (ja) | 1992-07-02 | 2001-01-15 | 石川島播磨重工業株式会社 | 偏平ブロー成形装置 |
| JP3196316B2 (ja) | 1992-05-20 | 2001-08-06 | 石川島播磨重工業株式会社 | ブロー成形装置 |
| JP3196323B2 (ja) | 1992-06-09 | 2001-08-06 | 石川島播磨重工業株式会社 | 偏平ブロー成形機のパリソン皺矯正装置 |
| EP4056708A1 (en) | 2010-01-19 | 2022-09-14 | DSM IP Assets B.V. | Isolated biomass from schizochytrium or thraustochytrium comprising epa and dha and its use in animal feed |
| AU2012285806B2 (en) | 2011-07-21 | 2017-09-14 | Dsm Ip Assets B.V. | Fatty acid compositions |
| US9587215B2 (en) | 2014-08-07 | 2017-03-07 | General Electric Company | Devices, systems and methods for automated transfer of a sample |
| BR112017006833B1 (pt) | 2014-10-02 | 2022-09-13 | Evonik Operations Gmbh | Alimento para animais contendo ácido graxo poli-insaturado e processo para produzir o mesmo |
| US20170295824A1 (en) | 2014-10-02 | 2017-10-19 | Evonik Degussa Gmbh | Process for producing a pufa-containing biomass which has high cell stability |
| ES2873094T3 (es) * | 2014-10-02 | 2021-11-03 | Evonik Operations Gmbh | Procedimiento para la producción de un pienso que contiene PUFAs mediante extrusión de una biomasa que contiene PUFAs de tipo Labyrinthulomycetes |
| DK179843B1 (en) | 2014-10-02 | 2019-07-31 | Evonik Degussa Gmbh | Method for raising animals |
| CN106433944A (zh) * | 2015-08-05 | 2017-02-22 | 嘉必优生物技术（武汉）股份有限公司 | 一种富含磷脂型多不饱和脂肪酸的微生物油脂的制备方法 |
| US11946017B2 (en) | 2016-07-13 | 2024-04-02 | Evonik Operations Gmbh | Method of separating lipids from a lysed lipids containing biomass |
| EP3485026A1 (en) * | 2016-07-13 | 2019-05-22 | Evonik Degussa GmbH | Method for isolating lipids from lipid-containing cells |
| BR112019003361A2 (pt) | 2016-08-19 | 2019-05-28 | Noblegen Inc | métodos e usos de frações de material orgânico dissolvido para ligação a íons metálicos |
| US11352651B2 (en) | 2016-12-27 | 2022-06-07 | Evonik Operations Gmbh | Method of isolating lipids from a lipids containing biomass |
| CA3059271A1 (en) | 2017-04-07 | 2018-10-11 | Noblegen Inc. | Methods and uses of encapsulated exudates and dried euglena biomass for binding metal |
| EP3470502A1 (en) | 2017-10-13 | 2019-04-17 | Evonik Degussa GmbH | Method of separating lipids from a lysed lipids containing biomass |
| EP3527664A1 (en) | 2018-02-15 | 2019-08-21 | Evonik Degussa GmbH | Method of isolating lipids from a lipids containing biomass |
| BR112020023222A2 (pt) | 2018-05-15 | 2021-03-23 | Evonik Operations Gmbh | método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão |
| CN112166176B (zh) | 2018-05-15 | 2024-06-25 | 赢创运营有限公司 | 借助疏水性二氧化硅从含有脂质的生物质中分离脂质的方法 |
| US20210277430A1 (en) * | 2020-03-09 | 2021-09-09 | Lanzatech, Inc. | Fermentation process for the production of lipids |
| EP4469588A1 (en) | 2022-01-25 | 2024-12-04 | DSM IP Assets B.V. | Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production |
| CN115927005B (zh) * | 2022-10-14 | 2024-11-22 | 广西中医药大学 | 两面针内生真菌y118的固体发酵培养基及其制备和培养方法 |
| WO2025019424A1 (en) * | 2023-07-14 | 2025-01-23 | Dsm Ip Assets B.V. | Microbial oils having high epa content and methods of making the same |
| CN118755584B (zh) * | 2024-08-06 | 2025-04-29 | 杭州合碳创物科技有限公司 | 一种高产epa的裂殖壶菌及其应用 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5130242A (en) | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| US6451567B1 (en) * | 1988-09-07 | 2002-09-17 | Omegatech, Inc. | Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms |
| DE69435246D1 (de) * | 1993-06-09 | 2009-12-03 | Martek Biosciences Corp | Verwendung von Docosahexaensäure zur Herstellung eines Arzneimittels zur Behandlung der senilen Demenz und Alzheimer-Erkrankung |
| DE69637953D1 (de) | 1995-04-17 | 2009-07-30 | Nat Inst Of Advanced Ind Scien | Hoch ungesättigte fettsäurenproduzierende mikroorganismen und verfahren zur herstellung von hoch ungesättigten fettsäuren durch verwendung dieser mikroorganismen |
| US6255505B1 (en) * | 1996-03-28 | 2001-07-03 | Gist-Brocades, B.V. | Microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
| PT996740E (pt) | 1997-08-01 | 2005-11-30 | Martek Biosciences Corp | Composicoes nutritivas contendo dha e metodos para a sua producao |
| ES2735987T3 (es) | 2000-01-19 | 2019-12-23 | Dsm Ip Assets Bv | Procedimiento de extracción sin disolvente |
| ES2653545T3 (es) | 2000-01-28 | 2018-02-07 | Dsm Ip Assets B.V. | Producción mejorada de lípidos que contienen ácidos grasos polienoicos por cultivos de alta densidad de microorganismos del orden Thraustochytriales en fermentadores |
| US6410282B1 (en) * | 2000-03-30 | 2002-06-25 | Council Of Scientific And Industrial Research | Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid fungi |
| DE10102337A1 (de) * | 2001-01-19 | 2002-07-25 | Basf Plant Science Gmbh | Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte |
| US20040203120A1 (en) * | 2002-03-22 | 2004-10-14 | Council Of Scientific And Industrial Research | Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists |
| JP4280158B2 (ja) | 2002-12-27 | 2009-06-17 | 富士フイルム株式会社 | ドコサヘキサエン酸生産能を有する微生物及びその利用 |
| US20050019880A1 (en) * | 2003-03-31 | 2005-01-27 | Council Of Scientific And Industrial Research | Method of enhancing levels of polyunsaturated fatty acids in thraustochytrid protists |
| EP1706500B1 (en) * | 2003-12-30 | 2010-09-08 | DSM IP Assets B.V. | Deaeration process |
| US7807849B2 (en) * | 2004-04-22 | 2010-10-05 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
| AU2007351658B2 (en) * | 2006-08-01 | 2013-05-30 | Dsm Nutritional Products Ag | Oil producing microbes and methods of modification thereof |
| CN103168860A (zh) * | 2007-08-31 | 2013-06-26 | 马太克生物科学公司 | 含多不饱和脂肪酸的固体脂肪组合物及其制备与应用 |
| CN101889068A (zh) * | 2007-09-11 | 2010-11-17 | 蓝宝石能源公司 | 用光合生物生产有机产物的方法及其产物和组合物 |
| CA2699406C (en) * | 2007-09-12 | 2019-09-03 | Martek Biosciences Corporation | Biological oils and production and uses thereof |
| EP2195415A1 (en) * | 2007-10-03 | 2010-06-16 | E. I. du Pont de Nemours and Company | Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
| CN109609562A (zh) * | 2009-03-19 | 2019-04-12 | 帝斯曼知识产权资产管理有限公司 | 破囊壶菌、脂肪酸组合物和其制备方法及用途 |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| US20110195449A1 (en) * | 2009-12-28 | 2011-08-11 | Martek Biosciences Corporation | Recombinant Thraustochytrids that Grow on Sucrose, and Compositions, Methods of Making, and Uses Thereof |
| EP4056708A1 (en) * | 2010-01-19 | 2022-09-14 | DSM IP Assets B.V. | Isolated biomass from schizochytrium or thraustochytrium comprising epa and dha and its use in animal feed |
| US20110263709A1 (en) * | 2010-04-22 | 2011-10-27 | E. I. Du Pont De Nemours And Company | Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass |
| US8769868B2 (en) * | 2010-06-09 | 2014-07-08 | Photonz Corporation Limited | Compositions comprising eicosapentaenoic acid suitable for high purification |
| ES2857173T3 (es) * | 2011-07-21 | 2021-09-28 | Dsm Ip Assets Bv | Aceites microbianos enriquecidos en ácidos grasos poliinsaturados |
-
2012
- 2012-07-20 WO PCT/US2012/047728 patent/WO2013013208A2/en not_active Ceased
- 2012-07-20 MY MYPI2014700130A patent/MY169454A/en unknown
- 2012-07-20 KR KR1020147004428A patent/KR102038715B1/ko active Active
- 2012-07-20 SG SG10201605966TA patent/SG10201605966TA/en unknown
- 2012-07-20 CN CN201280035935.2A patent/CN104011217B/zh active Active
- 2012-07-20 BR BR122015020130A patent/BR122015020130A2/pt not_active Application Discontinuation
- 2012-07-20 CA CA2842374A patent/CA2842374C/en active Active
- 2012-07-20 KR KR1020197019037A patent/KR102158437B1/ko active Active
- 2012-07-20 MX MX2017012687A patent/MX385408B/es unknown
- 2012-07-20 CN CN202411870122.6A patent/CN119899748A/zh active Pending
- 2012-07-20 AU AU2012285803A patent/AU2012285803B2/en active Active
- 2012-07-20 BR BR112014001443-4A patent/BR112014001443B1/pt active IP Right Grant
- 2012-07-20 CN CN201510526715.5A patent/CN105255738B/zh active Active
- 2012-07-20 EP EP25170782.4A patent/EP4585049A2/en active Pending
- 2012-07-20 PH PH1/2014/500169A patent/PH12014500169B1/en unknown
- 2012-07-20 EP EP12815192.5A patent/EP2734630B1/en active Active
- 2012-07-20 EP EP20207686.5A patent/EP3831952A1/en active Pending
- 2012-07-20 ES ES12815192T patent/ES2774778T3/es active Active
- 2012-07-20 ES ES15171724T patent/ES2859752T3/es active Active
- 2012-07-20 DK DK15171724.6T patent/DK3050972T3/da active
- 2012-07-20 AR ARP120102657A patent/AR087944A1/es unknown
- 2012-07-20 EP EP15171724.6A patent/EP3050972B1/en active Active
- 2012-07-20 EA EA201400151A patent/EA034980B1/ru not_active IP Right Cessation
- 2012-07-20 JP JP2014521849A patent/JP6338285B2/ja active Active
- 2012-07-20 MX MX2014000759A patent/MX351174B/es active IP Right Grant
- 2012-07-20 US US13/554,975 patent/US9045785B2/en active Active
-
2014
- 2014-01-16 IL IL230500A patent/IL230500B/en active IP Right Grant
- 2014-01-21 CL CL2014000156A patent/CL2014000156A1/es unknown
- 2014-01-24 ZA ZA2014/00620A patent/ZA201400620B/en unknown
- 2014-12-10 US US14/566,500 patent/US20150094382A1/en not_active Abandoned
-
2015
- 2015-04-23 ZA ZA2015/02764A patent/ZA201502764B/en unknown
- 2015-04-28 US US14/698,378 patent/US20150223492A1/en not_active Abandoned
- 2015-05-07 CL CL2015001217A patent/CL2015001217A1/es unknown
-
2016
- 2016-11-10 AU AU2016256769A patent/AU2016256769B2/en active Active
-
2017
- 2017-05-18 US US15/598,812 patent/US20170245523A1/en not_active Abandoned
- 2017-05-22 JP JP2017100780A patent/JP2017200480A/ja active Pending
-
2019
- 2019-11-07 JP JP2019202514A patent/JP7134938B2/ja active Active
-
2021
- 2021-11-05 US US17/519,961 patent/US20220322705A1/en active Pending
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