MX343755B - Mutantes de o-fosfoserina sulfhidrilasa y método para producción de cisteína usando los mismos. - Google Patents
Mutantes de o-fosfoserina sulfhidrilasa y método para producción de cisteína usando los mismos.Info
- Publication number
- MX343755B MX343755B MX2013004509A MX2013004509A MX343755B MX 343755 B MX343755 B MX 343755B MX 2013004509 A MX2013004509 A MX 2013004509A MX 2013004509 A MX2013004509 A MX 2013004509A MX 343755 B MX343755 B MX 343755B
- Authority
- MX
- Mexico
- Prior art keywords
- cysteine
- opss
- mutant
- production
- mutants
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01065—O-Phosphoserine sulfhydrylase (2.5.1.65)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a un mutante de sulfhidrilasa 0-fosfoserina (OPSS) el cual tiene una secuencia de aminoácidos derivada de Mycobacterium smegmatis correspondiente a la de SEQ ID NO: 1 que carece de tres a siete residuos de aminoácidos del terminal C. También, una molécula de ácido nucleico codificando el mutante OPSS, un vector de expresión transportando la molécula de ácido nucleico, y un transformante transformado con el vector de expresión se describen. Además, se proporciona un método para producir cisteína en el cual la O-fosfo-L-serina (OPS) reacciona con un sulfuro en la presencia del mutante de OPSS. El mutante de OPSS ha mejorado su actividad enzimática y puede aplicarse a la producción amigablemente ambiental de L-cisteína a través de una reacción simple de conversión enzimática.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020100102665A KR101208267B1 (ko) | 2010-10-20 | 2010-10-20 | Ｏ-포스포세린 설피드릴라제 변이체 |
| PCT/KR2011/007678 WO2012053777A2 (en) | 2010-10-20 | 2011-10-14 | O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013004509A MX2013004509A (es) | 2013-05-30 |
| MX343755B true MX343755B (es) | 2016-11-18 |
Family
ID=44789375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013004509A MX343755B (es) | 2010-10-20 | 2011-10-14 | Mutantes de o-fosfoserina sulfhidrilasa y método para producción de cisteína usando los mismos. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9127324B2 (es) |
| EP (2) | EP2444486B1 (es) |
| JP (1) | JP5789670B2 (es) |
| KR (1) | KR101208267B1 (es) |
| CN (2) | CN104762273A (es) |
| AU (1) | AU2011318793B2 (es) |
| BR (1) | BR112013009633B1 (es) |
| DK (2) | DK2796549T3 (es) |
| ES (2) | ES2533561T3 (es) |
| LT (1) | LT2796549T (es) |
| MX (1) | MX343755B (es) |
| PL (2) | PL2444486T3 (es) |
| RU (1) | RU2541782C2 (es) |
| TR (1) | TR201807729T4 (es) |
| WO (1) | WO2012053777A2 (es) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101525663B1 (ko) * | 2013-05-10 | 2015-06-04 | 씨제이제일제당 (주) | 신규 ｏ-포스포세린 배출 단백질 및 이를 이용한 ｏ-포스포세린의 생산방법 |
| KR101493154B1 (ko) | 2013-05-10 | 2015-02-13 | 씨제이제일제당 (주) | 신규 ＲｈｔＢ 단백질 변이체 및 이를 이용한 Ｏ-포스포세린의 생산방법 |
| US9587159B2 (en) * | 2014-12-04 | 2017-03-07 | Baker Hughes Incorporated | Enzymes for removing sulfurous compounds in downhole fluids |
| KR101694632B1 (ko) * | 2015-09-11 | 2017-01-10 | 씨제이제일제당 (주) | 신규 ｏ-포스포세린 배출 단백질 변이체 및 이를 이용한 ｏ-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
| CN106434854A (zh) * | 2016-09-29 | 2017-02-22 | 北京世纪沃德生物科技有限公司 | 一种检测同型半胱氨酸的试剂盒 |
| KR101825310B1 (ko) | 2016-12-29 | 2018-03-15 | 씨제이제일제당 (주) | O-포스포세린을 생산하는 에스케리키아 속 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인을 생산하는 방법 |
| KR102025867B1 (ko) | 2017-07-13 | 2019-09-27 | 씨제이제일제당 주식회사 | 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법 |
| KR102016050B1 (ko) | 2018-03-20 | 2019-08-29 | 씨제이제일제당 주식회사 | 신규한 프로모터 및 이의 용도 |
| AR122565A1 (es) | 2020-06-09 | 2022-09-21 | Cj Cheiljedang Corp | Variante de proteína de exportación de o-fosfoserina y método de producción de o-fosfoserina, cisteína y sus derivados usando las mismas |
| FR3112549A1 (fr) | 2020-07-20 | 2022-01-21 | Arkema France | Procede ameliore de synthese de mercaptans fonctionnalises |
| KR102414743B1 (ko) | 2020-09-09 | 2022-06-29 | 씨제이제일제당 주식회사 | 신규 o-포스포세린 배출 단백질 및 이를 이용한 o-포스포세린, 시스테인 및 이의 유도체의 생산 방법 |
| FR3117115B1 (fr) | 2020-12-04 | 2023-05-05 | Arkema France | Procede de synthese de mercaptans fonctionnalises sous pression d’h2s |
| KR20220163754A (ko) | 2021-06-03 | 2022-12-12 | 씨제이제일제당 (주) | 신규한 YhhS 변이체 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
| KR20220166947A (ko) | 2021-06-11 | 2022-12-20 | 씨제이제일제당 (주) | 신규한 MdtH 변이체 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
| KR102654301B1 (ko) | 2021-06-23 | 2024-04-04 | 씨제이제일제당 주식회사 | NADH:quinone 산화환원효소의 발현이 조절된 재조합 미생물 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19726083A1 (de) | 1997-06-19 | 1998-12-24 | Consortium Elektrochem Ind | Mikroorganismen und Verfahren zur fermentativen Herstellung von L-Cystein, L-Cystin, N-Acetyl-Serin oder Thiazolidinderivaten |
| US20050255568A1 (en) | 2003-05-30 | 2005-11-17 | Bailey Richard B | Methods and compositions for amino acid production |
| RU2279477C2 (ru) * | 2003-12-05 | 2006-07-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Мутантная серинацетилтрансфераза, фрагмент днк, кодирующий мутантную серинацетилтрансферазу (варианты), бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина, и способ продукции l-цистеина |
| RU2275425C2 (ru) * | 2003-11-03 | 2006-04-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина и способ получения l-цистеина |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| KR20080028940A (ko) * | 2005-06-17 | 2008-04-02 | 마이크로비아 인코포레이티드 | 아미노산 및 대사산물의 개선된 생합성 방법 |
| EP2314710B1 (en) | 2006-01-04 | 2016-03-30 | Metabolic Explorer | Method for the production of methionine by culturing a microorganism modified to enhance production of cysteine |
-
2010
- 2010-10-20 KR KR1020100102665A patent/KR101208267B1/ko active IP Right Grant
-
2011
- 2011-10-14 CN CN201510105512.9A patent/CN104762273A/zh active Pending
- 2011-10-14 RU RU2013122808/10A patent/RU2541782C2/ru active
- 2011-10-14 JP JP2013534803A patent/JP5789670B2/ja active Active
- 2011-10-14 WO PCT/KR2011/007678 patent/WO2012053777A2/en active Application Filing
- 2011-10-14 AU AU2011318793A patent/AU2011318793B2/en active Active
- 2011-10-14 MX MX2013004509A patent/MX343755B/es active IP Right Grant
- 2011-10-14 CN CN201180002041.9A patent/CN102741400B/zh active Active
- 2011-10-14 BR BR112013009633-0A patent/BR112013009633B1/pt active IP Right Grant
- 2011-10-19 EP EP20110185853 patent/EP2444486B1/en active Active
- 2011-10-19 DK DK14171034.3T patent/DK2796549T3/en active
- 2011-10-19 ES ES11185853.6T patent/ES2533561T3/es active Active
- 2011-10-19 PL PL11185853T patent/PL2444486T3/pl unknown
- 2011-10-19 ES ES14171034.3T patent/ES2671642T3/es active Active
- 2011-10-19 PL PL14171034T patent/PL2796549T3/pl unknown
- 2011-10-19 LT LTEP14171034.3T patent/LT2796549T/lt unknown
- 2011-10-19 DK DK11185853T patent/DK2444486T3/en active
- 2011-10-19 EP EP14171034.3A patent/EP2796549B1/en active Active
- 2011-10-19 TR TR2018/07729T patent/TR201807729T4/tr unknown
- 2011-10-20 US US13/278,100 patent/US9127324B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2011318793B2 (en) | 2016-01-14 |
| PL2444486T3 (pl) | 2015-06-30 |
| DK2444486T3 (en) | 2015-01-19 |
| ES2533561T3 (es) | 2015-04-13 |
| DK2796549T3 (en) | 2018-06-14 |
| AU2011318793A1 (en) | 2013-05-02 |
| JP2014503184A (ja) | 2014-02-13 |
| US20120190080A1 (en) | 2012-07-26 |
| KR20120041073A (ko) | 2012-04-30 |
| MX2013004509A (es) | 2013-05-30 |
| US9127324B2 (en) | 2015-09-08 |
| CN104762273A (zh) | 2015-07-08 |
| KR101208267B1 (ko) | 2012-12-04 |
| PL2796549T3 (pl) | 2018-08-31 |
| EP2796549B1 (en) | 2018-03-07 |
| RU2013122808A (ru) | 2014-11-27 |
| EP2796549A1 (en) | 2014-10-29 |
| BR112013009633B1 (pt) | 2022-02-08 |
| WO2012053777A3 (en) | 2012-06-21 |
| CN102741400B (zh) | 2015-03-18 |
| CN102741400A (zh) | 2012-10-17 |
| BR112013009633A2 (pt) | 2016-07-19 |
| TR201807729T4 (tr) | 2018-06-21 |
| ES2671642T3 (es) | 2018-06-07 |
| JP5789670B2 (ja) | 2015-10-07 |
| LT2796549T (lt) | 2018-07-10 |
| EP2444486A1 (en) | 2012-04-25 |
| WO2012053777A2 (en) | 2012-04-26 |
| RU2541782C2 (ru) | 2015-02-20 |
| EP2444486B1 (en) | 2015-01-07 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant or registration |