MX2013004509A - Mutantes de o-fosfoserina sulfhidrilasa y metodo para la produccion de cisteina usando los mismos. - Google Patents

Mutantes de o-fosfoserina sulfhidrilasa y metodo para la produccion de cisteina usando los mismos.

Info

Publication number
MX2013004509A
MX2013004509A MX2013004509A MX2013004509A MX2013004509A MX 2013004509 A MX2013004509 A MX 2013004509A MX 2013004509 A MX2013004509 A MX 2013004509A MX 2013004509 A MX2013004509 A MX 2013004509A MX 2013004509 A MX2013004509 A MX 2013004509A
Authority
MX
Mexico
Prior art keywords
cysteine
opss
mutant
production
mutants
Prior art date
Application number
MX2013004509A
Other languages
English (en)
Other versions
MX343755B (es
Inventor
An Shin Soo
Won Um Hye
Sook Chang Jin
Hyun Jo Jae
Min Lee Kyoung
Cheol Song Byeong
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of MX2013004509A publication Critical patent/MX2013004509A/es
Publication of MX343755B publication Critical patent/MX343755B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01065—O-Phosphoserine sulfhydrylase (2.5.1.65)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describe un mutante de sulfhidrilasa 0-fosfoserina (OPSS) el cual tiene una secuencia de aminoácidos derivada de Mycobacterium smegmatis correspondiente a la de SEQ ID NO: 1 que carece de tres a siete residuos de aminoácidos del terminal C. También, una molécula de ácido nucleico codificando el mutante OPSS, un vector de expresión transportando la molécula de ácido nucleico, y un transformante transformado con el vector de expresión se describen. Además, se proporciona un método para producir cisteína en el cual la O-fosfo-L-serina (OPS) reacciona con un sulfuro en la presencia del mutante de OPSS. El mutante de OPSS ha mejorado su actividad enzimática y puede aplicarse a la producción amigablemente ambiental de L-cisteína a través de una reacción simple de conversión enzimática.
MX2013004509A 2010-10-20 2011-10-14 Mutantes de o-fosfoserina sulfhidrilasa y método para producción de cisteína usando los mismos. MX343755B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100102665A KR101208267B1 (ko) 2010-10-20 2010-10-20 Ｏ-포스포세린 설피드릴라제 변이체
PCT/KR2011/007678 WO2012053777A2 (en) 2010-10-20 2011-10-14 O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same

Publications (2)

Publication Number Publication Date
MX2013004509A true MX2013004509A (es) 2013-05-30
MX343755B MX343755B (es) 2016-11-18

Family

ID=44789375

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013004509A MX343755B (es) 2010-10-20 2011-10-14 Mutantes de o-fosfoserina sulfhidrilasa y método para producción de cisteína usando los mismos.

Country Status (15)

Country Link
US (1) US9127324B2 (es)
EP (2) EP2444486B1 (es)
JP (1) JP5789670B2 (es)
KR (1) KR101208267B1 (es)
CN (2) CN104762273A (es)
AU (1) AU2011318793B2 (es)
BR (1) BR112013009633B1 (es)
DK (2) DK2444486T3 (es)
ES (2) ES2671642T3 (es)
LT (1) LT2796549T (es)
MX (1) MX343755B (es)
PL (2) PL2444486T3 (es)
RU (1) RU2541782C2 (es)
TR (1) TR201807729T4 (es)
WO (1) WO2012053777A2 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101525663B1 (ko) * 2013-05-10 2015-06-04 씨제이제일제당 (주) 신규 ｏ-포스포세린 배출 단백질 및 이를 이용한 ｏ-포스포세린의 생산방법
KR101493154B1 (ko) 2013-05-10 2015-02-13 씨제이제일제당 (주) 신규 ＲｈｔＢ 단백질 변이체 및 이를 이용한 Ｏ-포스포세린의 생산방법
US9587159B2 (en) * 2014-12-04 2017-03-07 Baker Hughes Incorporated Enzymes for removing sulfurous compounds in downhole fluids
KR101694632B1 (ko) * 2015-09-11 2017-01-10 씨제이제일제당 (주) 신규 ｏ-포스포세린 배출 단백질 변이체 및 이를 이용한 ｏ-포스포세린, 시스테인 및 이의 유도체의 생산방법
CN106434854A (zh) * 2016-09-29 2017-02-22 北京世纪沃德生物科技有限公司 一种检测同型半胱氨酸的试剂盒
KR101825310B1 (ko) * 2016-12-29 2018-03-15 씨제이제일제당 (주) O-포스포세린을 생산하는 에스케리키아 속 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인을 생산하는 방법
KR102025867B1 (ko) 2017-07-13 2019-09-27 씨제이제일제당 주식회사 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법
KR102016050B1 (ko) 2018-03-20 2019-08-29 씨제이제일제당 주식회사 신규한 프로모터 및 이의 용도
WO2021251734A1 (ko) 2020-06-09 2021-12-16 씨제이제일제당 (주) O-포스포세린 배출 단백질 변이체 및 이를 이용한 o-포스포세린, 시스테인 및 이의 유도체의 생산방법
FR3112549A1 (fr) 2020-07-20 2022-01-21 Arkema France Procede ameliore de synthese de mercaptans fonctionnalises
KR102414743B1 (ko) 2020-09-09 2022-06-29 씨제이제일제당 주식회사 신규 o-포스포세린 배출 단백질 및 이를 이용한 o-포스포세린, 시스테인 및 이의 유도체의 생산 방법
FR3117115B1 (fr) 2020-12-04 2023-05-05 Arkema France Procede de synthese de mercaptans fonctionnalises sous pression d’h2s
KR20220163754A (ko) 2021-06-03 2022-12-12 씨제이제일제당 (주) 신규한 YhhS 변이체 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법
KR102707457B1 (ko) 2021-06-11 2024-09-20 씨제이제일제당 주식회사 신규한 MdtH 변이체 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법
KR102654301B1 (ko) 2021-06-23 2024-04-04 씨제이제일제당 주식회사 NADH:quinone 산화환원효소의 발현이 조절된 재조합 미생물 및 이를 이용한 O-포스포세린, 시스테인 및 이의 유도체의 생산방법
KR102688937B1 (ko) 2021-12-31 2024-07-29 씨제이제일제당 주식회사 O-포스포세린 생산 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인 생산 방법
KR20230138803A (ko) 2022-03-24 2023-10-05 씨제이제일제당 (주) 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법

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DE19726083A1 (de) 1997-06-19 1998-12-24 Consortium Elektrochem Ind Mikroorganismen und Verfahren zur fermentativen Herstellung von L-Cystein, L-Cystin, N-Acetyl-Serin oder Thiazolidinderivaten
US20050255568A1 (en) 2003-05-30 2005-11-17 Bailey Richard B Methods and compositions for amino acid production
RU2279477C2 (ru) * 2003-12-05 2006-07-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Мутантная серинацетилтрансфераза, фрагмент днк, кодирующий мутантную серинацетилтрансферазу (варианты), бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина, и способ продукции l-цистеина
RU2275425C2 (ru) * 2003-11-03 2006-04-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина и способ получения l-цистеина
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
AU2006261356A1 (en) * 2005-06-17 2006-12-28 Microbia, Inc. Improved amino acid and metabolite biosynthesis
WO2007077041A1 (en) 2006-01-04 2007-07-12 Metabolic Explorer Process for the preparation of methionine and its precursors homoserine or succinylhomoserine employing a microorganism with enhanced sulfate permease expression

Also Published As

Publication number Publication date
JP2014503184A (ja) 2014-02-13
ES2671642T3 (es) 2018-06-07
EP2796549B1 (en) 2018-03-07
WO2012053777A3 (en) 2012-06-21
DK2444486T3 (en) 2015-01-19
BR112013009633A2 (pt) 2016-07-19
EP2444486B1 (en) 2015-01-07
AU2011318793B2 (en) 2016-01-14
WO2012053777A2 (en) 2012-04-26
CN104762273A (zh) 2015-07-08
BR112013009633B1 (pt) 2022-02-08
US20120190080A1 (en) 2012-07-26
ES2533561T3 (es) 2015-04-13
JP5789670B2 (ja) 2015-10-07
TR201807729T4 (tr) 2018-06-21
EP2444486A1 (en) 2012-04-25
US9127324B2 (en) 2015-09-08
MX343755B (es) 2016-11-18
DK2796549T3 (en) 2018-06-14
KR101208267B1 (ko) 2012-12-04
CN102741400A (zh) 2012-10-17
LT2796549T (lt) 2018-07-10
PL2796549T3 (pl) 2018-08-31
RU2013122808A (ru) 2014-11-27
KR20120041073A (ko) 2012-04-30
EP2796549A1 (en) 2014-10-29
CN102741400B (zh) 2015-03-18
RU2541782C2 (ru) 2015-02-20
AU2011318793A1 (en) 2013-05-02
PL2444486T3 (pl) 2015-06-30

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